Fig. 8

Altered GPLα expression results in modified Pi levels in Arabidopsis leaves. Total phosphorus (P) and inorganic phosphorus (Pi) content was measured in leaves of miR827- and GPLα-modified plants grown hydroponically. The Pi and total P contents were measured in 28-day-old plants. a Left panel: total P measured in miR827-overexpressing lines (miR827–OE1 and 2), wild type (Col-0, WT), and miR827-suppressed lines (miR827–STTM1 and 2). Right panel: Pi measured in these same miR827-modified lines. b Left panel: total P measured in GPLα-overexpressing lines (GPLα–OE1 and 2), WT (Col-0), GPLα-suppressed lines (GPLα–SI1 and 2), and GPLα-mutant line (GPLα–KO1). Right panel: Pi measured in these same GPLα-modified lines. c Left panel: total P measured in GPLα-overexpressing lines (GPLα–OE3 and 4), WT (Ler), GPLα-suppressed lines (GPLα–SI3 and 4), and GPLα-mutant line (GPLα–KO2). Right panel: Pi measured in these same GPLα-modified lines. Three independent transgenic lines were examined. Different letters above the columns indicate significant differences (P < 0.05, ±SD)