Fig. 4.

Regulation of PTEN/DAF-18 and AKT. a Mean percentage and SD of hermaphrodite and female F1 offspring from hermaphrodites treated with chemicals that activate or inhibit PTEN/DAF-18. (+) CM represents undiluted conditioned medium. The DMSO control and indole-3-carbinol (I3C) incubations were performed with diluted (1:10) CM. b Quantification of antibody staining with PTEN/DAF-18 in the maternal gonads. c Effect of pharmacological inhibition or activation of AKT on sexual morph ratios in the F1s. d Quantification of antibody staining for AKT pThr308 in the meiotic portion of the germline. e Quantification of premeiotic germline cells with staining with an antibody against AKT pThr308. Graphical representation in b, d as in Fig. 2b, and in e as in Fig. 3e. One-way analysis of variance (ANOVA) followed by a post hoc Holm-Sidak test for multiple comparisons (a inhibition of PTEN; c inhibition of AKT), Student’s t test (a activation of PTEN with I3C; c inhibition of AKT with perifosine), Mann-Whitney test (c activation of AKT with SC-79). *p ≤ 0.05, **p ≤ 0.01; ***p ≤ 0.001