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Table 1 Staging criteria for the specimens utilised in this study

From: The dorsoanterior brain of adult amphioxus shares similarities in expression profile and neuronal composition with the vertebrate telencephalon

Nomenclature HPF @ 20C Morphological criteria
8s 24h 8 somite embryos
1 GS 48h Larvae with a well-formed first primary gill slit
2.5 GS 60h Larvae with a well-formed first primary gill slit and the primordia of the second primary gill slit
5GS 3 months Larvae with 5 primary gill slits
9-10 GS 6 months Pre-metamorphic larvae with 10 primary gill slits**
  1. HPF hours post-fertilisation
  2. **B. lanceolatum larvae initiate metamorphosis when they have between 11 and 12 primary gill slits