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Table 1 Staging criteria for the specimens utilised in this study

From: The dorsoanterior brain of adult amphioxus shares similarities in expression profile and neuronal composition with the vertebrate telencephalon

Nomenclature

HPF @ 20C

Morphological criteria

8s

24h

8 somite embryos

1 GS

48h

Larvae with a well-formed first primary gill slit

2.5 GS

60h

Larvae with a well-formed first primary gill slit and the primordia of the second primary gill slit

5GS

3 months

Larvae with 5 primary gill slits

9-10 GS

6 months

Pre-metamorphic larvae with 10 primary gill slits**

  1. HPF hours post-fertilisation
  2. **B. lanceolatum larvae initiate metamorphosis when they have between 11 and 12 primary gill slits