Fig. 3.

A Maximum likelihood analysis of the available sequences of the subfamily Fasciolinae using the GTR + G (= 0.25) model on 814 bp of the COI marker. Fasciolopsis buski (“GenBank: NC_030528”), from the subfamily Fasciolopsinae, was used as an outgroup. B The Fasciola nyanzae COI haplotype network of 11 sequences based on 412 positions, visualized using the TCS model in PopArt®. One hatch mark represents one mutation. The legend indicates the used color-code and haplotype abundance through circle size. C Maximum likelihood analysis of the available sequences of the subfamily Fasciolinae using the GTR + G (= 0.05) model on 2771 bp of the rDNA region. Fasciolopsis buski (“GenBank: MN970005”), from the subfamily Fasciolopsinae, was used as an outgroup. Nodal support is indicated as bootstrap percentages (1000 bootstraps) and posterior probabilities, respectively before and after the “/” separator. Specimens from this study are indicated in blue. GenBank accession numbers are provided after the “|” separator