Fig. 7
Antennal and behavioral responses of A. segetum to medium-chain fatty acids. A Representative electrophysiological responses of male and female A. segetum antennae to medium-chain fatty acids. Top: GC trace of the saturated C6-C10 acids abbreviated as 6:0, 7:0, 8:0, 9:0, and 10:0 (FID). Bottom: antennal responses to corresponding compounds (EAD). B GC-EAD responses of male and female A. segetum to C6-C10 acids. Values are amplitude of EAD responses (mV, mean ± SE) after being normalized by corresponding FID peak abundance. Individual data values (the yellow and blue dots) were shown along with the chart. Statistically significant differences between stimuli within each sex are indicated by letters (one-way ANOVA followed by an LSD test at 0.05 level). Significant differences between males and females responding to the same stimulus are indicated by asterisks after Student’s t test at p < 0.05 level (two-sided). N = 6 for female, N = 4 for male. C Behavioral responses of female and male turnip moths in a Y-tube olfactometer to saturated fatty acids (with 6–10 carbon atoms) at the 100 μg dose, and D to octanoic acid at a series of doses from 100 ng to 100 μg. N value for each dual choice is shown on the right. Error bars indicate 95% confidence interval. P values are based on chi-square tests: N.S., P > 0.05; *, P < 0.05; **, P < 0.01