Fig. 2.
From: Decreasing dorsal cochlear nucleus activity ameliorates noise-induced tinnitus perception in mice
Inhibition of DCN CaMKII α-hM4Di-positive cells after noise exposure decreases tinnitus-like behavior. A Experimental timeline highlighting the GPIAS recordings before and after noise exposure. B Schematic drawing of the Startle and Gap-startle protocols. C Representative GPIAS recording of a mouse showing 68.69% suppression of acoustic startle before and 10.67% suppression after noise exposure when comparing Startle (red) and Gap-startle (black) responses, indicating tinnitus-like behavior for the tested frequency (9–11kHz). D, E GPIAS group performance before (blue) and after (orange) noise exposure for hM4Di (D) and eYFP (E) injected mice. F, G Left, histogram showing the number of hM4Di (F) and eYFP (G) injected animals in function of the frequency with the greatest decrease in GPIAS index. Right, GPIAS group index showed that hM4Di+ mice (F) decreased startle suppression after noise exposure and regained startle suppression under the effect of CNO, while eYFP-injected mice shows that, although presenting tinnitus-like responses after noise exposure, no difference was observed between NaCl and CNO treatments (p = 0.696). n = 11 and 7 for NE hM4Di+ and NE eYFP, respectively; *p >0.05; **p = 1.3e −10