Fig. 2

Classification of chronological aging mutants and Gene Ontology (GO) enrichment analysis. A, B Area distribution of the control strains used in the screening. Density plot showing the survival distribution of the three control strains: 666 (WT), sty1Δ (short-lived strain), and pka1Δ (long-lived strain). These distributions were considered to estimate the probability for every analyzed mutant to belong to each one of these groups. Once these posterior probabilities were calculated, each mutant was assigned to the category with the highest probability. The density plot (B) shows the resulting classification of mutants based on this approach. C, D Gene Ontology term enrichment. The long-lived (309) and short-lived (163) mutants identified in the screening were selected for GO enrichment analysis. They were classified into Cellular Component (C) and Biological Process (D) GO terms using Metascape [64]. Each bar represents a GO term ordered by statistical significance (-log10 p value)