Fig. 10

Endosome-like organelle models in Giardia, Spironucleus and Tritrichomonas and proposed evolution of CLC. A Simplified cartoons of the endocytic and secretory pathway in Giardia lamblia (Giardiinae), Spironucleus sp. (Hexamitidae) and Tritrichomonas foetus (Parabasalia). PV/PECs are a hallmark of the Giardia lineage, while more canonical vesicular endosomes are present in both the Spironucleus lineage and Parabasalia. B Simplified evolutionary model for bona fide CLC. The last eukaryotic common ancestor possessed a bona fide CLC which was lost at the last Fornicata common ancestor. In at least two derived lineages—Giardinae and Spironucleus spp.—de novo protein analogue to CLC was acquired independently