Arthropods and inherited bacteria: from counting the symbionts to understanding how symbionts count

In our paper in 2008, we started from the premise that the bacteria we were studying were parasites of arthropod reproductive systems that spread using sex ratio distortion or cytoplasmic incompatibility as drive mechanisms. However, we noted that, in most cases, the nature of the interactions between these inherited bacteria (including Wolbachia) and their hosts was not known, and thus remained to be determined.

It is now clear that the heritable microorganisms we studied are not simply reproductive parasites. Wolbachia in arthropods has emerged as a conditional mutualist conferring advantages under certain environmental conditions. For instance,

Wolbachia increases fecundity of Drosophila melanogaster reared on iron-restricted or -overloaded diets, and can thus confer a direct fitness benefit during periods of nutritional stress [2]. The most dramatic findings are that Wolbachia can protect their hosts against attack by natural enemies. Wolbachia infection interferes with the replication and transmission of a wide range of pathogens and parasites (including RNA viruses, bacteria, protozoa and nematodes), and protects its host from parasite-induced mortality [3]. These properties have led to Wolbachia being developed as an agent to limit transmission of human pathogens by arthropod vectors [4].

Wolbachia is not alone in being a defensive symbiont. Diverse symbionts in aphids provide protection against parasitic wasp and fungal attack, and include members of the Rickettsia and Spiroplasma genera [5, 6]. Drosophila, an organism intensively studied with respect to determinants of resistance to parasites, was recently revealed to have defensive Spiroplasma [7]. Paederus rove beetles carry heritable Pseudomonas that produces a toxin that deters predators [8]. Apart from protective effects, symbionts mediate variation in heat tolerance, plant use and body color [5, 9]. For instance, the symbiont Rickettsiella changes the body color of the aphid host from red to green, and is thus likely to influence relative susceptibility to predators [9].

It is also now clear that individual symbionts have multiple properties. For instance, the Wolbachia strain in D. melanogaster was characterized initially as one producing only weak reproductive manipulation but is now also known to confer nutritional and protective benefits. Similarly, Himler et al. [10] found that Rickettsia infection in whiteflies both increased host survival and reproductive success, and biased the sex ratio towards production of daughters, a classical feature of reproductive parasites. The further observation that some symbiont effects are revealed only in novel hosts suggests multiple potencies may be common [11]. Multiple effects on the host are also very important in the application of heritable microbes in disease control. The reproductive parasitism of Wolbachia allows it to invade and be maintained at high frequency in a population, such that the effect it has on the competence of individuals to act as disease-carrying vectors is then observed at a population scale [4].

Phylogenetic evidence indicates that most symbioses originate following horizontal transfer of an existing symbiont from one host species to another [12, 13]. It was always presumed that horizontal transfer events were rare, occurring on evolutionary rather than ecological timescales. However, heritable symbionts have been shown to combine inheritance with infectious transmission within, and sometimes between, species in a number of cases (Table 1). Ecological connections, such as feeding on a shared plant host, are major drivers for these rapid movements of symbionts across insect communities. Symbiont transfer between individuals of different host species will be an important determinant of the global incidence of infection. Further, the rate of horizontal transfer in some of these systems is such that a single host/single symbiont framework may be insufficient for understanding the population and evolutionary dynamics in some symbiotic systems.

Movement of symbionts and the traits they encode are now known to be very common through both inheritance and horizontal transfer. It is also beginning to emerge that other genetic connections are possible. Transfer of symbiont genetic information to the host's nuclear genome is known to occur frequently, although the functional significance of transferred material is less clear [14]. Symbionts can also exchange genetic information with other symbionts. Bacteria are, of course, promiscuous with respect to DNA, and different symbionts commonly reside within the same host cell, providing the opportunity for gene transfer. There is strong evidence Wolbachia exchange phage when two strains co-infect a host [15]. What is yet to be established is the extent of gene exchange between different heritable symbionts, and whether this leads to the transfer of traits such as natural enemy resistance. Comparisons of the genomes of Cardinium and Wolbachia strains inducing cytoplasmic incompatibility suggested many common mechanisms inherent in intracellular symbiosis between these very divergent bacteria, but no evidence that this was associated with gene exchange [16]. Nevertheless, phage can shuttle genes from one heritable bacterium species to another. Arsenophonus and Hamiltonella share a common phage, implying either direct transfer of the phage, or indirect transfer of genes within the phage through recombination of different phage elements [17]. Given that phage presence can determine the capacity of Hamiltonella to protect its aphid host against parasitoid wasp attack [18], phage transfers like this may move important traits between symbiont taxa.

Our understanding of the nature of host-inherited symbiont interactions has advanced since the advent of PCR led to the widespread discovery of Wolbachia and its domination of the literature. The case studies above demonstrate the importance of a diversity of symbionts as a source of evolutionary innovation in insects: symbionts alter host phenotype, and because they are heritable form part of host adaptation. Perhaps the most remarkable observation of recent time is the speed of symbiont-associated adaptation. Within 30 years, a Spiroplasma strain has invaded many North American populations of D. neotestacea, driven by the protection it provides against a parasitic nematode [7]. In whiteflies, an inherited Rickettsia strain that enhances offspring number and survival has spread from less than 1% of individuals infected to 97% in only 6 years [10]. Rapid spread of beneficial symbiont-encoded traits may be commonplace in insects. In this context, horizontal transfer through ecological interactions can serve as an immediate and powerful mechanism of rapid adaptation. The mutational source of adaptation - a symbiont in other members of the ecological community rather than a mutation of existing genetic material - is likely to change our understanding of arthropod evolution.

This article is part of the BMC Biology tenth anniversary series. Other articles in this series can be found at http://www.biomedcentral.com/bmcbiol/series/tenthanniversary.

Affiliations

1. Institut des Sciences de l'Evolution, Centre National de la Recherche Scientifique (CNRS), Université Montpellier 2, 34095, Montpellier Cedex 05, France

   • Olivier Duron

2. Institute of Integrative Biology, University of Liverpool, Liverpool, L69 7ZB, UK

   • Gregory DD Hurst

Corresponding author

Correspondence to Olivier Duron.

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