The comprehensive analysis of DEG/ENaC subunits in Hydrareveals a large variety of peptide-gated channels, potentially involved in neuromuscular transmission

Assmann, Marc; Kuhn, Anne; Dürrnagel, Stefan; Holstein, Thomas W; Gründer, Stefan

doi:10.1186/s12915-014-0084-2

Research article
Open Access
Published: 14 October 2014

The comprehensive analysis of DEG/ENaC subunits in Hydrareveals a large variety of peptide-gated channels, potentially involved in neuromuscular transmission

Marc Assmann¹,
Anne Kuhn²,
Stefan Dürrnagel¹,
Thomas W Holstein² &
Stefan Gründer¹

BMC Biology volume 12, Article number: 84 (2014) Cite this article

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Abstract

Background

It is generally the case that fast transmission at neural synapses is mediated by small molecule neurotransmitters. The simple nervous system of the cnidarian Hydra, however, contains a large repertoire of neuropeptides and it has been suggested that neuropeptides are the principal transmitters of Hydra. An ion channel directly gated by Hydra-RFamide neuropeptides has indeed been identified in Hydra - the Hydra Na⁺ channel (HyNaC) 2/3/5, which is expressed at the oral side of the tentacle base. Hydra-RFamides are more widely expressed, however, being found in neurons of the head and peduncle region. Here, we explore whether further peptide-gated HyNaCs exist, where in the animal they are expressed, and whether they are all gated by Hydra-RFamides.

Results

We report molecular cloning of seven new HyNaC subunits - HyNaC6 to HyNaC12, all of which are members of the DEG/ENaC gene family. In Xenopus oocytes, these subunits assemble together with the four already known subunits into thirteen different ion channels that are directly gated by Hydra-RFamide neuropeptides with high affinity (up to 40 nM). In situ hybridization suggests that HyNaCs are expressed in epitheliomuscular cells at the oral and the aboral side of the tentacle base and at the peduncle. Moreover, diminazene, an inhibitor of HyNaCs, delayed tentacle movement in live Hydra.

Conclusions

Our results show that Hydra has a large variety of peptide-gated ion channels that are activated by a restricted number of related neuropeptides. The existence and expression pattern of these channels, and behavioral effects induced by channel blockers, suggests that Hydra co-opted neuropeptides for fast neuromuscular transmission.

Background

In general, fast neurotransmission is mediated by small molecule neurotransmitters acting on ionotropic receptors at the post-synaptic membrane, while neuropeptides bind G-protein-coupled receptors (GPCRs) and mediate slow neuromodulatory transmission. So far, there are only two exceptions to this general pattern: the Phe-Met-Arg-Phe-amide (FMRFamide) activated Na⁺ channel (FaNaC) in snails [1],[2] and the RF-amide activated Hydra Na⁺ channel (HyNaC) [3],[4]. Both are ionotropic receptors, but their ligands are neuropeptides belonging to a family of FMRFamide-like peptides (FLPs) conserved from invertebrates to vertebrates [5]. Furthermore, FaNaC mediates fast responses of snail neurons to Phe-Met-Arg-Phe-amide (FMRFamide) [1],[6]-[8], a common neuropeptide in many nervous systems [9].

FaNaC and HyNaC both belong to the degenerin/epithelial Na⁺ channel (DEG/ENaC) gene family [10] but are not species orthologs. They rather belong to different branches of the DEG/ENaC family [3],[4]. DEG/ENaCs are cation channels that are blocked by the diuretic amiloride and share sequence homology and a secondary structure characterized by two transmembrane spanning domains (TMDs) and a large extracellular domain (ECD). They have various functions. Degenerins from Caenorhabditis elegans are mechanosensitive channels that mediate touch transduction [11]. In contrast, ENaC is a constitutively open channel that mediates Na⁺ reabsorption in the mammalian kidney [12]. Other family members from mammals are the acid-sensing ion channels (ASICs), ionotropic receptors for protons [13], and the bile-acid sensitive ion channel BASIC [14]. ASICs and BASIC are closely related to HyNaCs [3] and, interestingly, ASICs are modulated by direct binding of FLPs [15]. Pickpocket genes (PPKs) encode DEG/ENaCs in Drosophila and mediate diverse functions such as salt and water taste [16]-[18], mechanical nociception [19] and pheromone-sensing [20]-[22].

So far, four HyNaC subunits have been characterized: HyNaC2 to HyNaC5 [3],[4]; hynac1 probably is a pseudogene because it lacks an initiator methionine [3]. It is now accepted that DEG/ENaCs are trimers [23]-[25]; while FaNaC is a homotrimer [2], HyNaC is a heterotrimer consisting of three different subunits, HyNaC2, HyNaC3 and HyNaC5 [3],[4]. hynac2, hynac3 and hynac5 are co-expressed at the base of the tentacles, most likely in epitheliomuscular cells. Hydra-RFamides are expressed in neurons from the hypostomal and the peduncle region of Hydra[26],[27], where they are contained within large dense core vesicles in axon terminals of neurons contacting epitheliomuscular cells, suggesting that they contribute to neuromuscular transmission in Hydra[28],[29]. Since tentacle curling is delayed by amiloride [4], it has been proposed that HyNaC is involved in movement of the tentacles [4]. While hynac2 and hynac3 are uniformly expressed at the tentacle base [3],[4], hynac5 is restricted to the oral side [4], suggesting that HyNaC2/3/5 is active only in epitheliomuscular cells at the oral side of the tentacle base. In contrast, hynac4 localizes to the aboral side of the tentacles [3],[4]. HyNaC4 does not assemble with HyNaC2 and HyNaC3 in a functional channel [3], suggesting the existence of further HyNaC subunits at the aboral side of the tentacles.

Since Hydra belongs to the phylum Cnidaria, which is a sister phylum to all bilaterians, the distant relation of FaNaC and HyNaC suggests that the common ancestor to all DEG/ENaCs might have been a peptide-gated ion channel. Alternatively, peptide-gating might have evolved twice independently in the DEG/ENaC gene family. A more definite answer to this question needs a survey of the full repertoire of DEG/ENaCs in Hydra to establish whether all DEG/ENaCs in Hydra are peptide-gated channels. Moreover, the presence of many more neuropeptides in the Hydra nervous system [30] and the presence of the precursors that encode Hydra-RFamides I and II [27] at the peduncle region [31] raises the questions of the existence of HyNaCs that are gated by peptides other than Hydra-RFamides I and II or that are expressed at the peduncle and activated by Hydra-RFamides I and II.

Here, we report a comprehensive analysis of the DEG/ENaC gene family in Hydra. We show that there is a surprising variety of HyNaCs and that, with one possible exception, HyNaCs are heterotrimers and are activated by Hydra-RFamides I and II. Some HyNaCs are expressed at the peduncle where they could mediate fast responses to RFamides I and II. Moreover, we establish the rules by which the DEG/ENaC subunits in Hydra assemble into functional peptide-gated channels.

Results

The comprehensive analysis of DEG/ENaCs in Hydra

We cloned seven new cDNAs coding for proteins with high homology to the known HyNaCs (see Methods). We named these proteins HyNaC6 to HyNaC12. They have a size of 454 to 507 amino acids and a predicted molecular mass of 52.9 to 58.4 kDa. In general, the amino acid sequence identity of all HyNaCs varies between 29% and 65%. The exception is HyNaC12, which is only 14% to 17% identical to other HyNaCs. In addition, HyNaC3 and HyNaC11 are 85% identical to each other. Phylogenetic analyses revealed that HyNaC2 to HyNaC11 form a group of closely related channels within the DEG/ENaC gene family, suggesting that they derived from a single ancestor. HyNaC6 and HyNaC7 are more closely related to HyNaC2 and HyNaC5, whereas HyNaC8 to HyNaC11 are more closely related to HyNaC3 and HyNaC4, respectively, defining two subgroups within the HyNaC branch (highlighted by light and dark blue in Figure 1 and Additional file 1: Figure S1). Supporting previous results [3], HyNaC2 to HyNaC11 form a monophyletic group with ASIC and BASIC (see Figure 1 and Additional file 1: Figure S1). The grouping of HyNaCs and their relationship to ASICs and BASIC is apparent in the maximum likelihood analysis [see Additional file 1: Figure S1] and confirmed at a higher level of support by Bayesian analysis (Figure 1). The striking exception is HyNaC12, which is isolated from other HyNaCs or ASICs and BASIC (Figure 1). While the genes coding for HyNaC3 to HyNaC11 have introns, the sequence coding for HyNaC12 is present as a single uninterrupted open reading frame in the Hydra magnipapillata genome, showing that hynac12 is intronless, and suggesting that it derived by retrotransposition early in cnidarian evolution. Retrotransposition is relatively common in Hydra[32].

The database contains the first 150 amino acids of another predicted protein that shows high homology to HyNaCs but like HyNaC1 [3], HyNaC13 lacked an initiator methionine and we were not able to clone it from Hydra cDNA. Therefore, we conclude that hynac13 is an inactive pseudogene. Since extensive screening of the genomic database revealed no further DEG/ENaC homologs, we conclude that HyNaC2 to HyNaC12 constitute the whole DEG/ENaC gene family in Hydra magnipapillata.

All HyNaCs share conserved structures that are typical for DEG/ENaCs: they have short N- and C-termini, two TMDs and a large ECD between the TMDs [10]. Sequence motifs that are highly conserved in DEG/ENaCs are also at least partially present in HyNaCs; examples are the N-terminal HG motif [33], a di-arginine motif right before TMD1, a highly conserved tryptophane in TMD1 and the selectivity filter motif `GAS' in TMD2 [34],[35]. Conserved cysteine residues in the ECD [36] indicate a conserved tertiary structure (Figure 2).

New HyNaCs contribute to a variety of functional ion channels

We tested the function of the new HyNaCs by co-expression of different HyNaC subunit combinations in Xenopus oocytes. We screened for functional subunit combinations by application of 5 μM Hydra-RFamide I, a potent agonist of HyNaC2/3/5 [4]. When we expressed an individual HyNaC subunit we could never elicit currents. Similarly, the simultaneous expression of several cRNA never resulted in functional ion channels when HyNaC2 was absent. When HyNaC2 was part of the injected cRNA pool, however, Hydra-RFamide I elicited currents. Simultaneous expression of HyNaC2 with a single other HyNaC resulted only in a functional channel when we co-expressed HyNaC2 and HyNaC3; it was already known that HyNaC2 and HyNaC3 form a low-affinity peptide-gated channel [3]. Expression of HyNaC2 with two other HyNaCs, however, resulted in several functional ion channels (Figure 3).

Assembly of functional channels followed two simple rules. First, HyNaC2 had to be present. Second, one of the other two subunits had to be from subgroup 1 (dark blue in Figure 1) containing HyNaC5 to HyNaC7 and the other one from subgroup 2 (light blue in Figure 1) containing HyNaC3, HyNaC4 and HyNaC8 to HyNaC11 as defined by phylogenetic relationship (Figure 1). Specifically, HyNaC2 and HyNaC5 formed functional heteromers with HyNaC3 or HyNaC11, while HyNaC2 and HyNaC6 formed functional heteromers with all HyNaCs of the second subgroup. HyNaC2 and HyNaC7 formed functional heteromers with all members of the second subgroup except HyNaC8 (Figure 3).

The contribution of the distantly related HyNaC12 to peptide-gated channels was assessed by co-expression with HyNaC2 and one or several other HyNaCs (HyNaC3 to HyNaC11). In addition, HyNaC12 was co-expressed with pools of different HyNaCs without HyNaC2. In no case did this result in channels that could be activated by Hydra-RFamide I; in the case of HyNaC2/3, current amplitude and apparent peptide affinity were not increased by co-expression of HyNaC12. We conclude that HyNaC12 does not co-assemble with other HyNaCs into channels activated by Hydra-RFamide I. Since the closely related BASIC can efficiently be opened by lowering the concentration of extracellular divalent cations [37], reduction of extracellular divalent cation concentrations provides a potential means to open HyNaCs independently of specific agonists. In fact, all HyNaCs that were activated by Hydra-RFamide I were opened by lowering the extracellular Ca²⁺ concentration ([Ca²⁺]_e) to 10 nM (Figure 4). Moreover, HyNaC2/9/5, which was insensitive to Hydra-RFamide I, was also opened by lowering [Ca²⁺]_e, revealing that this combination of subunits assembles into a plasma membrane expressed ion channel, which was not gated by Hydra-RFamide I. In contrast, HyNaC12, whether expressed alone or in combination with other HyNaCs, was not opened by lowering [Ca²⁺]_e. Some DEG/ENaCs are constitutively opened by an amino acid substitution at the so-called DEG-position [38]. To further investigate whether HyNaC12 forms a functional homomeric channel, we substituted a glycine at the DEG-position by threonine (G436T), which has a large side chain. This substitution did, however, not constitutively open HyNaC12 (data not shown). This applies also for the co-expression of HyNaC12-G436T together with pools of different wild-type HyNaC subunits, leaving the function of HyNaC12 unknown.

Since Hydra-RFamide II had been shown previously to activate HyNaC2/3/5 [4], we tested Hydra-RFamides II to V for channel activation. All channels that were activated by Hydra-RFamide I were also activated by Hydra-RFamide II (data not shown). Instead Hydra-RFamide III to V (5 μM) failed to activate any functional HyNaC heterotrimer as well as HyNaCs containing HyNaC12 (Figure 4).

Expression pattern of HyNaCs reveals putative physiological subunit combinations

The expression patterns of hynacs were studied by in situ hybridization (ISH). It had already been shown that hynac2 to hynac5 are expressed at the base of the tentacles of adult animals [3],[4]. While hynac2 and hynac3 are uniformly expressed at the tentacle base, hynac4 and hynac5 are asymmetrically expressed. hynac5 is strongly expressed at the oral side of each tentacle base with a gradient toward the aboral side [4], and hynac4 expression is restricted to the aboral side of the tentacle base [3]. Similar to hynac4 and hynac5, hynac6 and hynac11 were asymmetrically expressed at the base of the tentacles (Figures 5 and 6). While expression of hynac11 was restricted to the oral side, similar to hynac5, expression of hynac6 was restricted to the aboral side (Figure 6), similar to hynac4. These findings, together with the formation of functional heteromeric channels in oocytes, suggest the presence of HyNaC2/3/5 and HyNaC2/11/5 heterotrimers at the oral side and of HyNaC2/3/6 and HyNaC2/4/6 heterotrimers at the aboral side of the tentacle base (Figure 3). The expression pattern is typical for expression in epitheliomuscular cells (for an example of hynac4 see Additional file 2: Figure S2).

The remaining hynacs were not expressed at the base of the tentacles (Figure 5). hynac7 and hynac10 were expressed at the peduncle (Figures 5 and 6), a region slightly above the basal disk. hynac10 was also expressed at the hypostome (oral region) (Figure 6). hynac8 and hynac9 were expressed over the whole body column but hynac9 had an increased expression level in the peduncle region (Figure 5). Since HyNaC2 was indispensable for formation of functional HyNaCs in the oocyte expression system, we re-evaluated its expression pattern and found slight expression of hynac2 over the whole body column including the peduncle (Figure 5). These findings suggest the presence of HyNaC2/9/7 and HyNaC2/10/7 heterotrimers at the peduncle region. Since neurons expressing the preprohormone A gene that encodes the Hydra-RFamides I to IV are present at the tentacle bases, hypostome, upper gastric region and peduncle [27],[31],[39], it is possible that cells expressing HyNaCs in the peduncle make synaptic junctions with neurons expressing Hydra-RFamides.

We were not able to detect hynac12 by in situ hybridization (Figure 5), suggesting that this subunit may be expressed in a restricted location or in low abundance. Moreover, hynac8 expression was faint and rather diffuse and we, therefore, could not assign a putative subunit combination containing HyNaC8. Figure 3 summarizes the putative physiological subunit combinations of HyNaCs.

HyNaCs have high-affinity for Hydra-RFamide I and II

We determined for HyNaCs with an overlapping expression pattern, and hence a putative occurrence in vivo, apparent affinity for Hydra-RFamide I. HyNaC2/3/5 has a high Ca²⁺ permeability, which leads to activation of Ca²⁺-activated chloride channels (CaCCs) that are endogenously expressed in Xenopus oocytes [40]. A biphasic current with an initial peak and a slowly developing sustained phase is indicative for activation of CaCCs. All new HyNaCs had the same biphasic current as HyNaC2/3/5 (Figure 7B), suggesting high Ca²⁺ permeability. Therefore, we determined apparent affinity for Hydra-RFamide I with oocytes that had been injected with the Ca²⁺-chelator ethylene glycol tetraacetic acid (EGTA). In the presence of intracellular EGTA, Hydra-RFamide I induced simple on- and off-responses, demonstrating that under these conditions currents were not strongly contaminated by currents through CaCCs. The EC₅₀ for Hydra-RFamide I varied over more than two orders of magnitude from 0.04 ± 0.01 μM (n = 9) for HyNaC2/9/7 to 13.8 ± 1.9 μM (n = 12) for HyNaC2/11/5; for comparison, HyNaC2/3/5 has an EC₅₀ of approximately 5 μM [4]. For HyNaC2/10/7, the apparent affinity for Hydra-RFamide I was too low to calculate the EC₅₀ precisely, since a saturating ligand concentration could not be reached (Figure 7C, Table 1).

Table 1 Summary of the biophysical properties of putatively physiological HyNaCs

Full size table

Previously, it had been shown that HyNaC2/3/5 has a ten times higher apparent affinity for Hydra-RFamide II than for Hydra-RFamide I [4]. We re-assessed apparent affinity of HyNaC2/3/5 for Hydra-RFamide II with EGTA-injected oocytes and found it to be 1.61 ± 0.27 μM (n = 14), slightly lower than reported for oocytes not injected with EGTA (0.34 ± 0.08 μM) [4]. Regarding the other putative physiological HyNaC heterotrimers, the EC₅₀ for Hydra-RFamide II varied between 0.04 ± 0.01 μM (n = 8) for HyNaC2/9/7 and 0.95 ± 0.16 (n = 7) for HyNaC2/11/5 (Figure 8), which is in the same range as for Hydra-RFamide I. Only for HyNaC2/11/5 was the apparent affinity for Hydra-RFamide II about 15-fold higher than for Hydra-RFamide I (0.95 μM compared with 13.8 μM).

Similar to HyNaC2/3/5 [4], all HyNaCs had a slightly positive reversal potential E_rev in standard bath (E_rev varied between 7.4 ± 0.5 mV, n = 10, for HyNaC2/9/7 and 17.3 ± 1.1 mV, n = 8, for HyNaC2/11/5; Figure 7D, Table 1), suggesting that they are unselective cation channels. HyNaC 2/3/5 is characterized by a high Ca²⁺ permeability with a permeability ratio P_Ca/P_Na = 3.85 [40]. We investigated the Ca²⁺ permeability of the new HyNaCs by replacing extracellular Na⁺ by Ca²⁺. An increase in extracellular Ca²⁺ from 1 to 10 mM resulted in a strong rightward shift of E_rev (Figure 9), confirming Ca²⁺ permeability of HyNaCs. The shift was in the same range as for HyNaC2/3/5 [40], suggesting a comparable Ca²⁺ permeability. We used E_rev at 10 mM Ca²⁺ and at 140 mM Na⁺ as the only permeant cations (see Methods) to calculate the relative permeability P_Ca/P_Na for the new HyNaCs. P_Ca/P_Na ranged from 2.66 for HyNaC2/4/6 (n = 9) to 3.72 for HyNaC2/3/6 (n = 9) (Table 1), showing that all HyNaCs are highly Ca²⁺ permeable.

Diminazene is a potent inhibitor of HyNaCs and inhibits the feeding reaction

One of the hallmarks of DEG/ENaCs is the block by the diuretic amiloride [10]. Since the apparent amiloride affinity of HyNaC2/3/5 is quite low with an IC₅₀ of approximately 120 μM [4], amiloride is of limited utility for the study of HyNaCs. Recently, diminazene and related compounds have been reported to potently inhibit ASICs and BASIC but not ENaC [41],[42]. Since ASICs and BASIC are closely related to HyNaCs, we asked whether diminazene also inhibits HyNaCs. For most HyNaCs, 10 μM of diminazene indeed almost completely blocked HyNaC currents. The IC₅₀ for diminazene varied between 0.05 ± 0.02 μM (n =9) for HyNaC2/4/6 and 1.70 ± 0.58 μM (n =9) for HyNaC2/9/7. Only HyNaC2/11/5 was blocked by diminazene with a low apparent affinity and an IC₅₀ of 31.4 ± 9.4 μM (n =8). Although we did not determine apparent amiloride affinity for all HyNaCs, based on a 20% to 70% inhibition of currents by 100 μM amiloride (Figure 10, Table 1) one can estimate that diminazene has a >100-fold higher potency than amiloride for most HyNaCs.

It has previously been shown that 100 μM amiloride delays the feeding reaction of living Hydra[4], supporting the hypothesis that amiloride-sensitive channels are involved in the feeding reaction. To find further evidence that amiloride inhibited the feeding reaction via inhibition of HyNaCs, we repeated these experiments with diminazene. Addition of glutathione (GSH; 10 μM final concentration) induced tentacle contractions and animals moved the tentacles to their mouth. After about two minutes, 100% of animals had their tentacles completely curled. When animals were held in a medium containing 100 μM diminazene, tentacle curling was slightly delayed. Increasing the concentration of diminazene to 200 μM, more strongly and significantly (P ≤0.01) delayed the initiation of tentacles movement such that tentacles were completely curled only after three minutes. When the concentration of diminazene was further increased to 300 μM, it strongly inhibited the feeding reaction such that even after four minutes only 20% of the animals had their tentacles completely curled (P ≤0.001; Figure 11). Although the potency of diminazene in vivo was low, it should be taken into account that septate junctions provide a significant paracellular permeability barrier to the mesogleal space of Hydra[43]. Moreover, it appears that diminazene requires active transport mechanisms for cellular uptake [44],[45], probably strongly limiting its transcellular transport; amiloride in contrast is, in part, uncharged at neutral pH and has a significant membrane permeability [46]. It is, therefore, conceivable that diminazene reached significantly lower concentrations in the mesogleal space of Hydra than in the bath. Therefore, these results support the idea that HyNaCs are involved in the feeding reaction of Hydra.

Discussion

DEG/ENaCs in Hydra are peptide-gated

In this study, we characterized the complete gene set of ten DEG/ENaC genes in the model cnidarian Hydra. We found genomic evidence for two further transcribed genes, that is, hynac1 and hynac12. Our data indicate, however, that these are pseudogenes that probably arose by retrotransposition from a common precursor with other HyNaCs [32]. The remaining ten genes give rise to ten HyNaC subunits that assemble in a variety of combinations into heteromeric ion channels.

HyNaCs are more closely related to each other than to any other DEG/ENaC, forming a sub-branch on the phylogenetic tree in Figure 1. Closest relatives are ASICs [13] and BASIC [14]. HyNaCs form two lower order subgroups within the HyNaC branch (Figure 1). Subgroup 1 (dark blue in Figure 1) contains HyNaC2, HyNaC5, HyNaC6 and HyNaC7, while subgroup 2 (light blue in Figure 1) contains HyNaC3, HyNaC4 and HyNaC8 to HyNaC11. Combinatorial expression of individual HyNaCs revealed the rules for subunit assembly. HyNaC2 was present in all functional channels. The second subunit had to be from subgroup 2 and the third was another subunit from subgroup 1, closely related to HyNaC2. HyNaC2/3 is the only HyNaC that contains only two different subunits yet is functional [3]. The presence of a HyNaC2-like subunit in all other channels makes 2/2/3 (and not 2/3/3) the likely composition of HyNaC2/3.

According to the rules described above, HyNaCs could theoretically assemble in 18 different combinations (Figure 3). We found that 13 of these combinations are activated by Hydra-RFamides I and II (Figure 3). One other combination (HyNaC2/9/5) was not activated by Hydra-RFamides I and II but by removing extracellular divalent cations, indicating correctly assembled plasma membrane expressed channels. It is therefore possible that HyNaC2/9/5 is activated by another neuropeptide than RFamides. Since the other four theoretical subunit combinations could neither be activated by RFamide neuropeptides nor by removal of divalent cations, it is likely that these combinations do not assemble in functional ion channels; at least not in the oocyte expression system. With the exception of HyNaC2/10/7, the functional peptide-activated channels all had high apparent ligand affinity; for some, such as HyNaC2/9/7, nanomolar concentrations of Hydra-RFamides I and II elicited robust currents (Figure 7). The high affinity for RFamides I and II renders it highly unlikely that other neuropeptides also activate HyNaCs with similar affinity. We, therefore, conclude that the physiological ligands of HyNaCs are Hydra-RFamides I and II, with the possible exceptions of HyNaC2/9/5 and HyNaC2/10/7. This conclusion lends support to the hypothesis that the common ancestor of DEG/ENaCs was a peptide-gated channel. A definite answer to this question, however, requires the analysis of DEG/ENaCs from more non-bilaterian animals.

HyNaCs may contribute to neuromuscular transmission in Hydra

For six of the thirteen heterotrimers, which are activated by RFamides in the oocyte expression system, the three subunits are co-expressed (Figure 5). It is therefore likely that these six heterotrimers form peptide-gated channels in situ. Due to the limited sensitivity of ISH, we cannot exclude that further combinations exist in situ. In particular, we could not assign the locus of expression of HyNaC8. In summary, our results suggest that two different HyNaCs are present at the oral (HyNaC2/3/5 and HyNaC2/11/5) and two at the aboral side of the tentacle base (HyNaC2/3/6 and HyNaC2/4/6), respectively. Two other HyNaCs are expressed at the peduncle (HyNaC2/9/7 and HyNaC2/10/7). Since these channels are all activated by Hydra-RFamides I and II, this variety is unexpected. We speculate that the variety of HyNaCs helps to fine tune ligand affinity and perhaps subcellular location of RFamide-gated channels.

What is the function of HyNaCs? ISH from this and previous [3],[4] studies suggests that HyNaCs are expressed in epitheliomuscular cells, which function as muscle cells. Since Hydra-RFamides are contained within large dense core vesicles in axon terminals of neurons contacting epitheliomuscular cells [28],[29], our results identify HyNaCs as a candidate post-synaptic receptor for Hydra-RFamides, which could depolarize muscle cells to induce contractions. In agreement with this speculation, amiloride [4] and diminazene, blockers of HyNaCs, significantly delayed the feeding reaction of Hydra (Figure 11). In vertebrates and arthropods, however, neuromuscular transmission uses the small molecule transmitters, acetylcholine and glutamate, respectively. Sequencing of the Hydra genome indeed revealed seventeen genes coding for nicotinic acetylcholine receptor subunits but other key components of the vertebrate neuromuscular junction are not present in Hydra and the authors concluded that a canonical bilaterian neuromuscular junction was not present in the last common ancestor of cnidarians and bilaterians [32]. Moreover, it has recently been suggested that striated muscles evolved convergently in cnidarians and bilaterians from cells with ancient contractile machinery [47]. Assuming that the junction with neurons of striated muscle cells evolved after or at the same time as the evolution of striated muscles, this finding would explain why cnidarian and bilaterian muscles might use different transmitters at their junction with neurons. In summary, our results suggest that Hydra-RFamides I and II and HyNaCs should be considered as a transmitter-receptor pair mediating fast neuromuscular transmission in Hydra.

One feature of HyNaCs that is unique for DEG/ENaCs is their high Ca²⁺ permeability. If HyNaCs indeed carried an excitatory post-synaptic current in muscle cells then Ca²⁺ flux through HyNaCs could directly induce muscle contractions independent of release from intracellular stores. A high Ca²⁺ permeability might also confer an advantage in a freshwater animal like Hydra with a low extracellular Na⁺ concentration. In Hydra littoralis (a member of the H. vulgaris group, [48]) an extracellular osmolality of 16.8 mM/kg has been measured [49], significantly higher than in the surrounding freshwater but much lower than in most other animals. Thus, Ca²⁺ may carry a significant fraction of the excitatory current in the freshwater animal Hydra.

Conclusions

Our study identifies in Hydra a variety of ion channels gated by Hydra-RFamide neuropeptides. Their expression in epithelial cells at the base of the tentacles and in the peduncle suggests a role for these channels in neuromuscular transmission. Thus, our results support the hypothesis that Hydra uses neuropeptides for fast neuromuscular transmission.

Methods

Cloning of new HyNaCs

Using the DNA sequences of HyNaC2 to HyNaC5, we performed a BLAST search against the genomic database of Hydra magnipapillata at the National Center for Biotechnology Information and identified the full-length protein sequence for three new HyNaCs; these HyNaCs were later named HyNaC6, HyNaC9 and HyNaC10, respectively. The complete coding sequences of these three HyNaCs were directly amplified by PCR with specific primers using cDNA extracted from adult one-day starved budding stage animals of Hydra magnipapillata strain 105. Other genomic sequences with homology to HyNaCs were used to design primers for 3'- and 5'-RACE. Sequences obtained by RACE-PCRs were assembled to full-length sequences, which were then amplified by PCR with specific primers using Hydra cDNA. The new HyNaC sequences were submitted to the EMBL-EBI-database with accession numbers HyNaC6 [EMBL:HG422725], HyNaC7 [EMBL:HG422726], HyNaC8 [EMBL:HG422727], HyNaC9 [EMBL:HG422728], HyNaC10 [EMBL:HG422729], HyNaC11 [EMBL:HG422730], HyNaC12 [EMBL:HG422731].

Analysis of phylogenetic relationship of HyNaCs

To analyze the phyologenetic relationship of HyNaC subunits, their sequences were aligned with the sequences of other DEG/ENaC channels using ClustalX (v.2.1); highly divergent sequences at the N- and C-terminus as well as in the extracellular loop were deleted to improve the alignment by automated 1 method of trimAl (v.1.3). The best model for amino acid substitution was determined with ProtTest 3 [50]. Phylogenetic trees were established by neighbor-joining analysis with ClustalX (v.2.1), maximum likelihood analysis with PhyML (v.3.0) [51] and Bayesian analysis with MrBayes (v.3.2). For maximum likelihood analysis we used the WAG model of protein evolution and the NNI tree topology search option; bootstrap support values were obtained from 100 bootstrap samples. During Bayesian analysis an average standard deviation ≤0.0001 was estimated. Therefore we ran 10⁷ generations with one chain without Metropolis coupling. After a burn-in of 10⁵ generations every 1,000th tree was sampled. Regarding the phylogenetic relations of HyNaCs, ASICs and BASICs, all three phylogenetic analyses revealed comparable results. Relations of other DEG/ENaCs were slightly different. A ClustalW analysis of all HyNaC subunits, rASIC1a, and rBASIC was performed with the software DNASTAR (v.10.0.1; Madison, WI, USA) to draw the alignment of Figure 2.

In situ hybridization

Expression patterns of HyNaCs were analyzed by whole mount in situ hybridization [52]. For all new HyNaCs, probes for in situ hybridization were subcloned into pBluescript KS; they had lengths between 600 and 1,400 bp, depending on the staining efficiency. Probes were detected with an antibody conjugated to an alkaline phosphatase and BMP Purple as substrate.

Electrophysiology

To study the biophysical properties of the new HyNaCs, cRNA of HyNaCs was synthesized in vitro (mMessage mMachine kit, Ambion, Austin, Texas, USA) and injected into Xenopus laevis oocytes of stage V and VI. After injection, oocytes were incubated for one to two days at 19°C in oocyte Ringer's solution 2 (OR-2), which contained (in mM): 82.5 NaCl, 2.5 KCl, 1.0 Na₂HPO₄, 1.0 MgCl₂, 1.0 CaCl₂, 5.0 HEPES, 0.5 g/l PVP, 1,000 U/l penicillin and 10 mg/l streptomycin, NaOH was used to adjust pH to 7.3. Whole cell currents were recorded from defolliculated oocytes by two-electrode voltage-clamp (TEVC) using the amplifier TurboTec 03X (npi electronic GmbH, Tamm, Germany). Standard bath solution (in mM: 140 NaCl, 10 HEPES, 1.8 CaCl₂ and 1.0 MgCl₂. NaOH was used to adjust pH to 7.4.) was exchanged by a pump driven system combined with the oocyte testing carousel (OTC), which was controlled by the interface OTC-20 [53]. To control the OTC-20 and to record the data to the hard drive, the software CellWorks (version 5.1.1; npi electronic GmbH, Tamm, Germany) was used. Data were sampled at 0.1 to 1 kHz and filtered at 20 Hz. HyNaCs are permeable for Ca²⁺, activating an endogenous CaCC [40]. Therefore, unless otherwise indicated, oocytes were injected with 50 nl of 20 mM EGTA 30 to 180 minutes before measurements. Reversal potentials and Ca²⁺ permeabilities of HyNaCs were studied as described previously [40].

Data analysis

EC₅₀ values for Hydra-RFamides I and II as well as IC₅₀ values for diminazene were determined by fits to the Hill equation using IGOR Pro (version 6.06, WaveMetrics, Inc.). Reversal potentials were defined as the first recorded data point, where currents reversed signs from negative to positive. For I/V relationships, voltage ramps in the absence of the agonist were used to subtract background conductances. Results are reported as mean ± standard error.

Authors' contributions

MA and SD carried out the molecular cloning of new HyNaC subunits. MA made the phylogenetic analyses, characterized HyNaCs electrophysiologically in Xenopus oocytes, performed the statistical analysis and participated in drafting the manuscript. AK carried out the in situ hybridizations and the pharmacological studies with live Hydra. TWH and SG conceived the study, and participated in its design and coordination and drafted the manuscript. All authors read and approved the final manuscript.

Additional files

Abbreviations

ASIC:: acid-sensing ion channel
BASIC:: bile acid-sensitive ion channel
CaCCs:: Ca⁺-activated chloride channels
DEG:: degenerin
ECD:: extracellular domain
EGTA:: ethylene glycol tetraacetic acid
ENaC:: epithelial Na⁺ channel
FaNaC:: FMRFamide-gated Na⁺ channel
HyNaC:: Hydra Na⁺ channel
ISH:: in situ hybridization
PPK:: pickpocket
TMD:: transmembrane domain

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Acknowledgements

We thank S. Lenz for expert technical assistance and Y. Moran (Hebrew University, Jerusalem) for advice on phylogenetic analysis. This project was supported by grants # GR 1771/7-1 and HO 1088/7-1 from the Deutsche Forschungsgemeinschaft to SG and TWH, respectively.

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Department of Physiology, RWTH Aachen University, Aachen, D-52074, Germany
Marc Assmann, Stefan Dürrnagel & Stefan Gründer
Molecular Evolution and Genomics, Center for Organismal Studies, Heidelberg, D-69120, Germany
Anne Kuhn & Thomas W Holstein

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Marc Assmann
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Stefan Dürrnagel
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Thomas W Holstein
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Figure S1.

Additional file 1: Phylogenetic tree for the DEG/ENaC family generated by maximum likelihood analysis. The phylogenetic tree was generated by maximum likelihood analysis with PhyML (see Methods). Support values are indicated. Scale bar indicates amino acid exchanges per site. Color code, abbrevations of species names, and accession numbers are as in Figure 1. (JPEG 785 KB)

Figure S2.

Additional file 2: High magnification of whole mount in situ hybridization for hynac4 revealing expression in epitheliomuscular cells at the base of the tentacles. (JPEG 82 KB)

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Assmann, M., Kuhn, A., Dürrnagel, S. et al. The comprehensive analysis of DEG/ENaC subunits in Hydrareveals a large variety of peptide-gated channels, potentially involved in neuromuscular transmission. BMC Biol 12, 84 (2014). https://doi.org/10.1186/s12915-014-0084-2

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Received: 09 July 2014
Accepted: 03 October 2014
Published: 14 October 2014
DOI: https://doi.org/10.1186/s12915-014-0084-2

Keywords

ASIC
DEG/ENaC
Evolution
Ligand-gated ion channel
Nervous system
Neuropeptide

The comprehensive analysis of DEG/ENaC subunits in Hydrareveals a large variety of peptide-gated channels, potentially involved in neuromuscular transmission

Abstract

Background

Results

Conclusions

Background

Results

The comprehensive analysis of DEG/ENaCs in Hydra

New HyNaCs contribute to a variety of functional ion channels

Expression pattern of HyNaCs reveals putative physiological subunit combinations

HyNaCs have high-affinity for Hydra-RFamide I and II

Diminazene is a potent inhibitor of HyNaCs and inhibits the feeding reaction

Discussion

DEG/ENaCs in Hydra are peptide-gated

HyNaCs may contribute to neuromuscular transmission in Hydra

Conclusions

Methods

Cloning of new HyNaCs

Analysis of phylogenetic relationship of HyNaCs

In situ hybridization

Electrophysiology

Data analysis

Authors' contributions

Additional files

Abbreviations

References

Acknowledgements

Author information

Affiliations

Corresponding author

Additional information

Competing interests

Electronic supplementary material

Authors’ original submitted files for images

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Keywords

BMC Biology

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