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  • Research article
  • Open Access

Nuclear fallout provides a new link between aPKC and polarized cell trafficking

  • 1,
  • 1,
  • 2,
  • 2,
  • 2 and
  • 1Email author
BMC Biology201614:32

https://doi.org/10.1186/s12915-016-0253-6

©  Calero-Cuenca et al. 2016

  • Received: 16 March 2016
  • Accepted: 31 March 2016
  • Published:

Abstract

Background

Cell polarity, essential for cell physiology and tissue coherence, emerges as a consequence of asymmetric localization of protein complexes and directional trafficking of cellular components. Although molecules required in both processes are well known their relationship is still poorly understood.

Results

Here we show a molecular link between Nuclear Fallout (Nuf), an adaptor of Rab11-GTPase to the microtubule motor proteins during Recycling Endosome (RE) trafficking, and aPKC, a pivotal kinase in the regulation of cell polarity. We demonstrate that aPKC phosphorylates Nuf modifying its subcellular distribution. Accordingly, in aPKC mutants Nuf and Rab11 accumulate apically indicating altered RE delivery. We show that aPKC localization in the apico-lateral cortex is dynamic. When we block exocytosis, by means of exocyst-sec mutants, aPKC accumulates inside the cells. Moreover, apical aPKC concentration is reduced in nuf mutants, suggesting aPKC levels are maintained by recycling.

Conclusions

We demonstrate that active aPKC interacts with Nuf, phosphorylating it and, as a result, modifying its subcellular distribution. We propose a regulatory loop by which Nuf promotes aPKC apical recycling until sufficient levels of active aPKC are reached. Thus, we provide a novel link between cell polarity regulation and traffic control in epithelia.

Keywords

  • aPKC
  • Cell polarity
  • Cell trafficking
  • Nuclear fallout

Background

Cells must be polarized to exert their functions. Establishment of cell polarity involves the trafficking machinery, which controls sorting of the molecules to their final destination, polarizing the organelles, the cortex and the extracellular membrane. The Par-6/Par-3/aPKC, the Crumbs/PATJ/PALS1 and the Scrib/Dlg/Lgl complexes control cell polarity and are conserved from invertebrates to vertebrates [1, 2]. Depending on the tissue, these complexes interact with different proteins. As a result, the mechanisms maintaining polarity vary from one tissue to another. These complexes also regulate cell signalling [3] and trafficking [2]. Thus, members of the polarity complexes modulate endocytosis [4] recycling and exocytosis [5–7]. However, how polarity complexes control trafficking routes and molecule delivery is mostly unknown.

We show that Nuclear Fallout (Nuf) and the atypical PKC (aPKC) are part of a new mechanism linking cell polarity with intracellular trafficking. Nuf belongs to the Rab11-family interacting proteins (FIPs), which bind and control the movement of the recycling endosome (RE) GTPase Rab11 through the microtubule cytoskeleton [8]. aPKC regulates cell polarity through the phosphorylation of various proteins modifying their behaviour or subcellular distribution. We demonstrate that aPKC phosphorylates Nuf affecting Nuf’s distribution and Rab11 and RE delivery. We show aPKC apico-lateral cortex levels are maintained by aPKC recycling in a Nuf-RE dependent manner. Thus, Nuf-aPKC act in a regulatory loop controlling Nuf-RE delivery and aPKC cortical levels.

Results

aPKC interacts and phosphorylates nuclear fallout

aPKC is a Ser/Thr kinase regulated by its interaction with other proteins [9]. In a Tandem Affinity Purification biochemical screen [8] to identify new aPKC partners/substrates we isolated Nuf (Fig. 1a and Additional file 1: Figure S1). GST pull-down assays using embryonic extracts and GST-Nuf recombinant protein confirm a strong interaction with endogenous aPKC (Fig. 1b). Pull-down assays of GST-Nuf and aPKC recombinant proteins indicate a direct interaction (Fig. 1c).
Fig. 1
Fig. 1

Nuf directly interacts with and is phosphorylated by aPKC. a aPKC full-length, regulatory and kinase domain Tetra-Tag (TT) constructs used in pull-down assays. b Pull-down (PD) assay of wild-type embryonic extracts with GST or GST-Nuf. Immunoblotting of bound proteins with anti-aPKC show aPKC presence only in the Nuf-containing sample. c In vitro assay of aPKC binding to Nuf. Recombinant aPKC was incubated in vitro with recombinant purified GST or GST-Nuf. Immunoblotting with anti-aPKC shows direct binding of aPKC to Nuf. d The kinase domain of aPKC interacts with Nuf. Embryonic extracts were incubated with aPKC kinase (Flag-aPKCKD) or regulatory domain (Flag-aPKCRD) bound to magnetic beads. Immunoblotting with anti-Nuf shows Nuf only binds to the kinase domain. The observed higher molecular weight in Nuf bound to the kinase domain of aPKC is consistent with Nuf being phosphorylated by aPKC. Asterisks point to aPKC versions used in the pull-downs cross-reacting with anti-Nuf. e Nuf only binds active aPKC. Wild type kinase domain (Flag-aPKCKD) and inactive kinase version (Flag-aPKCKD-mut) were purified with magnetic beads from embryonic extracts before incubation with purified recombinant Nuf-GST. Immunoblotting of bound proteins with anti-Nuf shows interaction with the wild type kinase domain. f Amino acid sequence and schematic representation of Nuf full-length and variants fused to GST. Asterisk marks Nuf N terminal fragment end. Orange: coiled-coil domain (CBD). Pink: Rab11 binding domain (RBD). Ser155 is highlighted in red. g Autoradiography of Nuf-GST variants subjected to aPKCz phosphorylation in vitro. Only full-length and N-terminal Nuf were phosphorylated (red asterisks). Black asterisk marks auto-phosphorylated aPKC. Preference towards substrate phosphorylation caused a reduction in autophosphorylation activity of aPKC. h Nuf phosphorylation sites. In vitro aPKC kinase was unable to phosphorylate S155A. i Recombinant GST-wild-type (WT), non-phosphorylatable (S155A) or phosphomimetic (S155D) Nuf pull-down assays from embryonic extracts. Immunoblotting probed with anti-aPKC shows Nuf S155D cannot interact with aPKC. “Input” contains 10 % (b, d, i) or 5 % (c) of the extracts. b-e, g-i. Lower panels are loading controls. Blots were probed with the indicated antibodies. aPKC atypical PKC, Nuf nuclear fallout, GST glutathione S-transferase

aPKC has an auto-regulatory pseudosubstrate domain (RD) and a kinase domain (KD) (Fig. 1a, [9]). The aPKC KD interacts with Nuf (Fig. 1d) only when active, as a kinase inactive version of aPKC [10] was unable to bind recombinant Nuf (Fig. 1e). This prompted us to investigated whether Nuf was phosphorylated by aPKC. Nuf belongs to the Arfophilin family of proteins, which are the targets of different kinases, IKK among them [11]. Nuf can be subdivided into an amino-terminal domain and a carboxy-terminal domain composed of a coiled-coil domain and the Rab11 binding domain (Fig. 1f). In vitro kinase assays showed the full-length Nuf and the amino-terminal domain (1–241) were phosphorylated by aPKC (Fig. 1g) correlating with the amino-terminal region of Nuf interacting with aPKC (Additional file 1: Figure S1). Interestingly, the human homolog Rab11-FIP3 is also phosphorylated by aPKC ζ and λ/ι, the two aPKC homologs in vertebrates (Additional file 1: Figure S1), indicating the conservation of the interaction and phosphorylation.

Nuf’s phosphorylation sites by aPKC were mapped in an in vitro kinase assay coupled to MS/MS detection of phosphopeptides. Phosphorylation sites at residues Ser155, Thr159, Ser175 and Ser180 were found (Additional file 1: Figure S1). We generated Nuf S155A, T159A, S175A or S180A single mutants and subjected them to aPKC in vitro phosphorylation. S155A abolished Nuf phosphorylation signal revealing S155 as the main phosphorylation target of aPKC in vitro (Fig. 1h).

Next we tested whether mutation of this serine residue modified Nuf interaction with aPKC. A phospho-mimetic mutation changing Ser155 to Aspartic (Nuf FL-S155D) was compared in pull-down assays with the full length and non-phosphorylatable versions of Nuf (Nuf FL-WT and Nuf FL-S155A, respectively). As with other aPKC substrates that lose affinity for the kinase once phosphorylated [12, 13], the phospho-mimetic version of Nuf did not bind to aPKC (Fig. 1i). These results identify Nuf as a novel aPKC substrate.

aPKC controls Nuf’s sub-cellular localization

Nuf is an adaptor of Rab11 to the dynein and kinesin microtubule motor complexes affecting RE distribution [8, 14]. To find out if aPKC regulated Nuf and Rab11 subcellular distribution we studied the imaginal wing disc pseudo-stratified epithelium (Fig. 2a-d). Nuf is found in a uniform punctate distribution in the wild-type disc (Fig. 2e). However, in mutant aPKC clones Nuf accumulates in the subapical region (Fig. 2e”). This accumulation is accompanied by Rab11 accumulation (Fig. 2e’).
Fig. 2
Fig. 2

Nuf subcellular distribution is modified by aPKC phosphorylation. a-b Third instar imaginal wing disc (a) and a section is shown in (b). c-d Representation of an apical (1) and a transversal view (2) showing in d the position of apical-lateral markers. e aPKC clones in wing discs (marked by the absence of aPKC, green) accumulate Rab11 (e’ and red in e) and Nuf (e” and blue in e) apically. f-h Subcellular distribution of wild-type (f), non-phosphorylatable (g) and phosphomimetic (h) Nuf in wing discs. Nuf driven by hh-Gal4 accumulates apically. NufS155A (g, magenta) reaches the apico-lateral membrane, marked by aPKC (green, co-localization in white). NufS155D is excluded from the apico-lateral membrane (h). In (f-h), upper panels show apical views, medial panels show sagittal views and lower panels are close-ups of the above. Yellow asterisks mark the apical distribution of the respective Myc-Nuf versions. Scale bar 10 μm. i Quantification of myc (red) compared to DPATJ (blue) levels in epithelia of nuf 1 homozygous wing disc expressing Myc-NufWT, Myc-NufS155A or Myc-NufS155D. Picks of DPATJ mark cell membranes. j Apico-cortical accumulation of NufS155A (red) is lost in aPKC-depleted cells marked by the absence of GFP (green, delimited by the yellow dotted line). Par3 (green) was used as membrane marker. k Quantification of Myc (red) and GFP/Par3 (blue) at the boundary between two clone cells (1) or two wild-type cells (2). Nuf nuclear fallout, aPKC atypical PKC, GFP green fluorescent protein.

In embryonic epithelia, loss of aPKC is related with loss of apico-basal polarity. However, the accumulation of Nuf and Rab11 in the sub-apical region suggests aPKC mutant wing disc cells conserve certain polarity. We confirmed this by looking at other cell polarity markers. At the moment when we analysed Nuf localization in aPKC clones, the adherens junctions markers were still localized at the membrane as shown by E-Cadherin (Additional file 2: Figure S2) or Par-3 (Additional file 2: Figure S2). The sub-apical membrane determinant Crb is lost from the membrane of most of the cells in the clone, however some of them still retain Crb in the membrane (Additional file 2: Figure S2). It is noteworthy that in null mutant clones for other polarity determinants, such as Crb or Par-3, aPKC localization was not severely disrupted and Nuf was unaffected (Additional file 2: Figure S2). These results point to aPKC loss as the direct cause for Nuf’s subapical accumulation.

To examine whether the phosphorylation state of Nuf affected its subcellular localization we compared the distribution of the non-phosphorylatable and phospho-mimetic Nuf mutants. When over-expressed in wing discs, all Nuf proteins accumulated apically (Fig. 2f-h). However, whilst non-phosphorylatable Nuf accumulated close to the apico-lateral membrane, partly co-localizing with aPKC (Fig. 2g), phospho-mimetic Nuf was excluded from the lateral membrane (Fig. 2h). This localization is independent of the endogenous Nuf as the same distribution is detected in a nuf homozygous background (Fig. 2i).

Thus, the difference in Nuf distribution relies on its aPKC-phosphorylation state. In some polarity processes, phosphorylation induces binding to the cytoplasmic protein 14-3-3 [15] resulting in cytoplasmic protein retention [16–19]. This was not the case for Nuf, as although NufWT and, to a lesser extent, NufS155A and NufS155D can bind 14-3-3 (Additional file 3: Figure S3) this binding was not modified by aPKC phosphorylation (Additional file 3: Figure S3).

We tested if the loss of affinity of phosphorylated Nuf for aPKC could be the cause for Nuf’s apical distribution by inducing aPKC null clones in cells expressing Myc-NufS155A. As shown in Fig. 2j-k, the cortical distribution of NufS155A becomes cytosolic in aPKC mutant cells. This and the biochemical data (Fig. 1i) indicate that aPKC controls the apical distribution of Nuf by inhibiting via phosphorylation its apico-lateral accumulation.

Non-phosphorylatable Nuf modifies aPKC apical levels

We examined whether aPKC affects Nuf localization by modifying its affinity for its known binding partners: Rab11 and the microtubule cytoskeleton motors dynein and kinesin [14, 20]. In pull-down assays we found Rab11 bound to wild type, non-phosphorylatable and phospho-mimetic Nuf with similar affinities. Similarly, Nuf phosphorylation did not affect its binding to kinesin and DLIC and dynactin, subunits of the dynein-dynactin complex. Moreover, these bindings were not competed by aPKC (Additional file 3: Figure S3).

To find if general delivery to the apico-lateral membrane was affected in Nuf mutants, we compared the distribution and levels of DE-Cad (a known RE cargo [21, 22]) and Par-3 markers of adherens junctions, and Crb and aPKC markers of the sub-apical region, in wing discs expressing UAS-Myc-NufS155A or UAS-Myc-NufS155D. While neither of these proteins modified the levels or localization of DE-Cad, Par3 or Crb, expression of the non-phosphorylatable NufS155A induced aPKC membrane accumulation (Fig. 3a). In contrast phosphomimetic NufS155D caused no aPKC accumulation (Fig. 3b). To discard a possible interference of endogenous Nuf protein in the phenotypes, we repeated the experiment in nuf hetero- and homozygous mutants. In both cases the increase in aPKC levels in cells over-expressing NufS155A was maintained (Fig. 3c-d and Additional file 4: Figure S4), but we did not observe any effect in the abundance or localization of the other markers (Fig. 3e-f and Additional file 4: Figure S4). These data indicate that the aPKC-dependent phosphorylation of Nuf exclusively prevents Nuf membrane localization without affecting its binding to the RE machinery and the RE delivery to the apico-lateral membrane and points to aPKC as a cargo of the RE.
Fig. 3
Fig. 3

NufS155A increases aPKC apico-lateral membrane levels. a-b Wing discs of wild-type larvae expressing in the posterior compartment Myc-NufS155A (a) or Myc-NufS155D (b) stained for aPKC (green) or Nuf (anti-Myc, magenta). Upper panels apical views, medial panels sagittal views and lower panels close-up of the above. Arrowheads and arrows point to cortical aPKC in cells located in anterior or posterior compartment of disc. c-f Quantification of aPKC (c-d) and DE-Cad (e-f) levels in epithelia of nuf 1 /+ heterozygous background wing disc expressing in the posterior Myc-NufS155A (c, e) or Myc-NufS155D (d, f). Fluorescence levels of aPKC or DE-Cad are shown comparing control anterior cells (orange and red) with posterior cells expressing Myc-NufS155A or Myc-NufS155D (blue). Nuf nuclear fallout, aPKC atypical PKC

aPKC is recycled through the RE

The higher levels of aPKC observed in epithelia expressing Nuf-S155A suggest an active transport of aPKC via RE. In fact, mammalian aPKC transport in Rab11 positive RE has been described during the first steps of polarization of the mammary gland and in 3D MDCK cultures where it is necessary for cysts formation [5, 23]. However, active transport of aPKC has not been described in established epithelia. Moreover, a truncated form of nuf (nuf BRW [24]) lacking the amino terminal region, therefore lacking the domain required for binding to and being phosphorylated by aPKC, overexpressed in a nuf mutant background accumulates apically colocalising with Rab11 (Additional file 5: Figure S5). In this condition aPKC can be detected in some of these Rab11 positive vesicles (Additional file 5: Figure S5). This data reinforces the transport of aPKC to the membrane via RE.

If aPKC transport to the apical membrane depends on RE, blocking exocytosis in mutants for sec6 or sec5 should increase cytosolic aPKC. Although sec6 mutant cells have very low viability, the few clones recovered accumulated aPKC and Nuf (Fig. 4a, red) and other recycling cargoes, such as Delta [25] and not shown). We obtained similar results with sec5 (Fig. 4b, blue). Accordingly, when eliminating Rab11 with a specific RNAi we observed aPKC was lost from the cell junctions and accumulated sub-apically inside the cell (Fig. 4c). Moreover, we found vesicles co-stained with aPKC and Rab5, an endosomes marker, supporting an active endocytosis of aPKC and reinforcing the idea of aPKC recycling (Fig. 4d).
Fig. 4
Fig. 4

aPKC is recycled via Nuf-RE. a-b Confocal images of wing discs containing sec6 (a) or sec5 (b) clones. In the absence of sec6 (a) aPKC accumulates in the mutant cells (red, right panel). In sec5 clones (b) aPKC (blue, right panel) and Nuf (red, middle panel) accumulate. c When Rab11 is silenced, aPKC (red) is disrupted from the apico-lateral cortex (arrows) accumulating in the cytoplasm (right). d Wing disc stained with aPKC (green) and the early recycling endosome marker Rab5 (red). Arrows point to co-stained endosomes. e-f aPKC (red) is reduced in nuf KG00314 (e) or nuf 1 (f) mutant clones. g-h Fluorescence levels of aPKC comparing wild-type (red) with clone cells (blue) for nuf KG00314 (g, left) or nuf 1 (h, right). Clones are marked by the absence of GFP (a and b) or β-gal (e-f) or expression of GFP (c) in green. (a-c, e-f) Lower panels show sagittal views of the clones and are marked by arrows. aPKC atypical PKC, RE recycling endosome, GFP green fluorescent protein

Considering that blocking exocytosis, interfering with sec6 or sec5, induces accumulation of aPKC, Rab11 and Nuf (Fig. 4 and [26]), that blocking the traffic of the RE by elimination of Rab11 generates aPKC accumulation in the cell and that aPKC accumulates in the apico-lateral cortex when a non-phosphorylatable version of Nuf is overexpressed, our data suggest an active movement of aPKC to the membrane through Nuf-positive RE. To confirm this hypothesis, we induced nuf 1 or nuf KG00314 mutant clones and observed a reduction of aPKC levels at the apical cortex (Fig. 4e-h), whilst other apical determinants as DPATJ or the adherens junctions markers Par3 and DE-Cad are unaffected (Additional file 6: Figure S6). Together, our data strongly indicate that aPKC is recycled to the membrane via Nuf-Rab11-RE.

Discussion

Recent research has shown the relationship between apico-basal cell polarity and polarized cellular trafficking. Here, we uncovered a novel direct link between apico-basal polarity and trafficking at the level of the RE, mediated by aPKC and Nuf. Nuf is phosphorylated by aPKC and this phosphorylation controls the trafficking of the RE to the apico-lateral domain. We also show an aPKC recycling mechanism dependent on Nuf-Rab11-RE exists in the mature epithelia of the imaginal wing disc. Nuf is a hyperphosphorylated protein. Recently Otani et al. demonstrated that the phosphorylation state of Nuf by IKKε regulates the subcellular distribution of Nuf and RE in the chaeta [11]. Here we unravel a new mechanism to control Nuf localization by aPKC-dependent phosphorylation.

aPKC function is mediated by phosphorylation of different substrates. In apico-basal polarity, aPKC refines the distribution of target proteins as Par-3/Baz or Crb [10, 18, 27] or locally excludes target proteins as Par-1, Pins, Lgl, Yurt or Numb [17, 19, 28–33]. Nuf distribution is also determined by aPKC-dependent phosphorylation. When aPKC is absent Nuf accumulates apically. Moreover, while the non-phosphorylatable Nuf accumulates adjacent to the lateral membrane the phosphomimetic version of Nuf is displaced from the apico-lateral cortex. This distribution is due to the binding affinity of Nuf to aPKC, as phosphomimetic NufS155D in pull-down assays cannot bind aPKC and avoids the apico-lateral cortex when expressed in imaginal discs whilst the non-phosphorylatable version NufS155A conserves the capacity to bind aPKC, accumulates in the apical cortex when expressed in epithelia and loses this distribution in the absence of aPKC. In fact, endogenous Nuf accumulates apically in aPKC mutant cells but avoids the apico-lateral cortex (Additional file 2: Figure S2) supporting the requirement of aPKC for apico-lateral distribution. However, in the wild type situation this interaction must be very transient as no membrane co-localization of aPKC and Nuf in the membrane can be detected. It has been described that aPKC phosphorylation can drive the interaction and retention in the cytoplasm of aPKC substrates by the protein 14-3-3/par5 [17, 19, 34, 35]. This is not the case with Nuf since, although Nuf binds 14-3-3 in vitro, aPKC dependent phosphorylation does not modify the binding. However, considering Nuf is a hyper-phosphorylated protein, 14-3-3 could modulate its function in other processes where Nuf could be involved. Interestingly, a function of 14-3-3 regulating Rab11 recycling endosomes has been described in Caenorhabditis elegans [36] and in Drosophila [37].

Considering that the main function of Nuf is to control the traffic of Rab11-associated recycling endosomes and aPKC phosphorylation or binding does not modify Nuf’s affinity for Rab11, dynein or kinesin complexes, by controlling Nuf’s subcellular distribution aPKC may affect the trafficking of Nuf-RE directed vesicles. Over-expression of different phosphorylated states of Nuf did not affect the distribution of DE-Cad, pointing to a DE-Cad-Rab11 recycling independent of Nuf in accordance with observations in the tracheal system where DE-Cad recycling depends on Rip11, the other arfophilin in Drosophila [22]. Par-3 distribution remains unaltered when NufS155A is overexpressed, suggesting that recycling of Par-3 is also Nuf independent. Our experiments did not detect any influence of Nuf on Crb localization. However, Crb membrane recycling has been shown to depend on the retromer and only in the newly synthetized membranes depends on Rab11 [2].

In contrast, the levels of aPKC depend on aPKC-Nuf interaction. Expression of NufWT or NufS115D do not alter the levels of aPKC, but expression of non-phosphorylatable NufS155A increases aPKC levels in the apico-lateral cortex. This suggests an active transport of aPKC to the apical membrane in Nuf-Rab11 RE. We obtained additional evidence in this direction by interfering with the general RE pathway overexpressing a truncated form of Nuf (NufBRW). We observed a significant increase in the number of Rab11 positive vesicles and the induction of strong phenotypes similar to Rab11-RNAi expression and suggesting a blockage of the recycling pathway. Under this condition, we observed a partial accumulation of aPKC in apical vesicles that are also Rab11 positive, further supporting a Nuf-dependent aPKC recycling mechanism (Additional file 5: Figure S5). However, unlike NufS155A, NufBRW does not accumulate in the membrane, neither aPKC membrane levels are modified, indicating a failure at some level in the fusion of the vesicles, maybe due to the inability of NufBRW to interact with the active aPKC in the membrane. The role of Nuf in the recycling of aPKC is consistent with the cytoplasmic accumulation of aPKC when we block exocytosis in exocyst mutants or RE-vesicle delivery by eliminating Rab11 (Fig. 4), and with the presence of vesicles co-stained with the endocytic endosome GTPase Rab5 and aPKC. Nuf mutant cells show lower levels of apical aPKC suggesting a Nuf-Rab11-RE-mediated transport of aPKC to the apical membrane and aPKC inhibits its own recycling by phosphorylation of Nuf displacing Nuf and Nuf associated-RE from the apico-lateral membrane (Fig. 5).
Fig. 5
Fig. 5

aPKC recycling in the epithelial cells. Schematic model of aPKC transport to the membrane in Rab11-Nuf RE vesicles (left): (1) Vesicles derived from the RE (REdv) containing aPKC move to the apico-lateral site via Rab11-Nuf. (2) aPKC interacts with the apical complexes and becomes active. (3) Active aPKC is able to interact with and phosphorylate Nuf. This phosphorylation release aPKC-Nuf interaction (red dash-line box on the right) (4) inhibiting its apico-lateral localization. aPKC atypical PKC, RE recycling endosome, Nuf nuclear fallout

Thus, although other scenarios are possible (i.e. a passive transport of aPKC due to interaction with other apical determinant actively transported via Rab11-RE or forming complex of the RE as a recycling regulator), all our data strongly point to an apical recycling of aPKC via Nuf-Rab11 in Drosophila. Moreover, we suggest that aPKC inhibits its own recycling by phosphorylation of Nuf, displacing Nuf and Nuf-associated RE from the apico-lateral membrane (Fig. 5).

Among the most common apical sorting signal is the presence of N-glycans attached to the protein. Interestingly, Drosophila aPKC has two N-glycosylation sites (position 18–21 and 226–229 respectively) as in vertebrates (aPKC λ position 18–21 and 241–244) that could be responsible for apical delivery. Also, our data suggest aPKC is a regulator of ARE-Rab11 delivery by controlling Nuf apical cortex interaction. It is not clear how Rab11 docks to and fuses with the membrane. aPKC could help via Nuf to Rab11 membrane docking. Recently, the SNARE interactor protein Munc13-4 has been involved in Rab11 docking to the membrane [38]. It would be interesting to investigate if the Drosophila homolog unc 13-4A also controls Rab11 docking and if a relationship with aPKC exists.

This aPKC-dependent mechanism for membrane recycling may be evolutionarily conserved since FIP3, the human ortholog of Nuf, interacts with, and is phosphorylated by, aPKCλ (Additional file 1: Figure S1). Also, the aPKC Rab11-FIP3-dependent recycling in epithelial cells may be preserved in mature epithelia and not only adscript to lumen formation.

Conclusions

Cells must be organized in different functional domains to maintain cell homeostasis and exert their functions. This is achieved through conserved proteins that polarize the cell or regulate directional trafficking of cellular vesicles. Both mechanisms must be coordinated but there is little information about their relationship. Here, we describe a new connexion between Nuf, an adaptor of Rab11-GTPase to the microtubule motor proteins in the recycling endosomes (RE) process, and aPKC, one of the main regulators of cell polarity. We demonstrate that aPKC phosphorylates Nuf, modifying Nuf subcellular distribution and, thus, RE delivery. We also show that aPKC’s apical recycling is maintained through Nuf-Rab11-RE. Thus, our results provide a novel link between cell polarity regulation and cellular traffic control.

Methods

Fly strains

We used the following stocks: Oregon R (wild-type), nuf 1 [39], nuf KG00314 (flybase), nuf BRW [24], FRTG13::aPKC K06403 , baz 4 ::FRT19a, FRT82B::crb 1 , sec6 ∆20 ::FRTG13,/+ [40], sec5 E15 ::FRT40A [26]. UAS-Rab11-RNAi (v22198) from the Vienna Drosophila RNAi Centre.

We used 69B-Gal4, 24B-Gal4, en-Gal4 and Hh-Gal4 as driver lines.

Immunohistochemistry

Imaginal discs were fixed for 20 minutes in paraformaldehyde 20 % followed by a second fixation of 20 minutes in paraformaldehyde 20 %–0.1 % TritonX-100.

The following primary antibodies were used: anti-Nuf (1:500, generated in this work as described in [11]), mouse and rabbit anti-aPKC (1:100 and 1:500, respectively) and rabbit anti-Myc (1:250) from Santa Cruz Biotechnology; mouse anti-Myc (Cell Signalling 1:500), rabbit anti-GFP (Molecular Probes, 1:300); anti-Dlg (1:100), anti-Crb (1:100) and anti-DECad (1:20) from Developmental Studies Hybridoma Bank, mouse anti-Myc (Cell Signalling, 1:500); anti-Rab11 (BD Biosciences, 1:100); anti-ßgal (Promega, 1:10.000), anti-Baz (1:500, this work), anti-DHC (1:500, [41]), anti-Rab5 (1:25, a gift from M. González-Gaitán) and anti-DPATJ (1:500, a gift from H. Bellen). Secondary antibodies were coupled to Alexa488, Alexa555 or Alexa647 (Molecular Probes).

Affinity-purified guinea pig antibody for Nuf was generated in this study by conventional methods. Briefly, E. coli BL21(DES)pLyS was transformed with pGEX-6P-1 Nuf FL and the protein overexpression was performed at 20 °C with 0.1 mM IPTG for 20 hours. The recovered cell pellets were lysed by sonication in PBS, 1 mM DTT and 1X complete protease inhibitors cocktail (Roche) buffer. After sonication, the lysate was incubated on ice with 1 % Triton-X100 for 30 minutes. The lysate was centrifuged (14,000 rpm, 4 °C, 30 minutes) and incubated with glutathione-Sepharose beads (Sigma) for 4 hours at 4 °C, the beads were washed three times with PBS and 1 mM DTT and twice with PBS, 1 mM DTT and 1 M NaCl. The protein bound to the beads was eluted with elution buffer (50mM Tris HCl pH 8.5, 0.1 %, Triton X-100, 100 mM NaCl, 1 mM DTT, 20 mM glutathione). The eluted material was lyophilised and sent to Genosphere Biotech to generate the antibody.

Affinity-purified rabbit antibody for Baz/Par-3 was generated in this study by transformation of BL21(DES)pLyS with pGEX-6P-1-Baz N1 [42] and following the same protocol as above with anti-Nuf.

Images were taken on a SP2-AOBS or a SPE Leica confocal microscopes and processed using FIJI and Adobe Photoshop programs.

Quantification of antibody staining and statistics

A total of fifteen confocal images of 0.16 μm thickness of a wing imaginal disc were projected using the average intensity algorithm (ImageJ) and five cells were randomly selected in the anterior and posterior compartments. The fluorescence intensity (pixel grey intensity shown in arbitrary units) was measured along a line 3 μm long. Different samples (n = 5) were measured for each genotype (n = 25).

In Additional file 4: Figure S4d the differences between fluorescence at the membrane and the cytoplasm were calculated for each cell analysed (n = 25) in anterior or posterior and in the different backgrounds as indicated. Data are displayed as a box plot and the statistical significance was calculated using the non-parametric Wilcoxon test.

To analyse Nuf distribution (Fig. 2i) 15 confocal images of 0.16 μm thickness of a wing imaginal disc expressing the different Nuf transgenes were projected using the average intensity algorithm (ImageJ). A total of five cells were randomly selected and five different samples were measured for each genotype (n = 25 each). The myc fluorescence intensity (pixel grey intensity shown in arbitrary units) was measured along a line 3 μm long that encompassed the two opposite membrane cells. Myc fluorescence was compared to PATJ, an apico-lateral protein of the Crb complex.

Figure 2k shows the fluorescence levels of myc-NufSA, measured as described above, in the border between two aPKC mutant cells (1) or two sibling wild type cells (2). The border cell was defined and measured by Par-3 levels. Keep in mind that Par-3 and GFP (marker of wild type cells) are stained in green indicating the higher basal levels of Par3 in wild type cells.

Pull-down assays and Western blots

To perform pull down experiments Nuf-GST fusion proteins were obtained from subclones in pGEX-6P-1 (Nuf FL) or pGEX2T (Nuf-NH and Nuf-CO). Purification was done as described in [11]. A total of 300 μg of protein extract from Drosophila embryos in lysis buffer (50 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EGTA, 2 mM EDTA, 1 % Triton X-100, 5 mM DTT, 1 mM PMSF, 1 mM sodium orthovanadate, 1X complete protease inhibitor cocktail (Roche) and 10 mM β-glycerol phosphate) were incubated for 4 hours at 4 °C with 30 μg of glutathione-S-transferase (GST) or of the different GST-fusion proteins bound to glutathione beads as described in [10]. Complexes were washed three times using 50 mM Tris HCl pH 7.5, 50 mM NaCl and 0.1 % Triton X-100 and once with 50 mM Tris HCl pH 6.8. In vitro pulldown was performed incubating 20 μg of GST or Nuf-GST proteins bound to glutathione beads with 0.25 μg of PKCz (PRKCz Recombinant Human Protein, Life Technologies) in lysis buffer for 2 hours at 4 °C. Complexes were washed three times using PBS 1X supplemented with 0.5 M NaCl and once with 50 mM Tris HCl pH 6.8. The GST-tag of proteins expressed from pGEX-6P-1 plasmids was removed by PreScission protease (GE Healthcare) following the manufacturer’s protocol and used in vitro for the Rab11-GST pull-down assay as described in [10].

Expression and purification of the recombinant proteins GST-14-3-3ε and GST-DLIC was performed as described above for Nuf from pGEX-6P-1-14-3-3 and pGEX-6P-1-DLIC respectively.

Competitive pull-down assays

Kinesin and dynactin: 20 μg of GST, GST-NufNH wt, GST-NufNH S155A and GST-NufNH S155D fusion proteins were incubated with 300 μg of OR extract for 3 hours at 4 °C in lysis buffer (50 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EGTA, 2 mM EDTA, 1 % Triton X-100, 5 mM DTT, 1 mM PMSF, 1 mM sodium orthovanadate, 1X complete protease inhibitor cocktail (Roche) and 10 mM β-glycerolphosphate). Beads were washed three times with 50 mM Tris HCl pH 7.5, 50 mM NaCl and 0.1 % Triton X-100 and incubated with 0, 50 or 200 μg of PKCz (PRKCz recombinant human protein, Life Technologies) for 1 hour at 4 °C. Beads were washed three times with IP15OO buffer and once with Tris HCl 50 mM pH 6.8.

GST-Rab11 and GST-DLIC: 20 μg of GST and GST-fusion proteins were incubated with 200 ng of NufFL wt, NufFL S155A and NufFL S155D proteins (GST was removed with Prescission protease, GE Healthcare) for 2 hours at 4 °C in Rab11 interaction buffer (PBS, 2 mM DTT, 1 mM sodium orthovanadate, 1 mM PMSF, 1X complete protease inhibitor cocktail) and DLIC interaction buffer (50 mM Tris HCl pH 7.5, 0.5 % Triton X-100, 150 mM NaCl, 10 % glycerol and 1 mM EDTA) respectively. Beads were washed three times with Rab11 interaction buffer supplemented with 50 mM NaCl for Rab11 assay and DLIC interaction buffer for DLIC assay. After that, beads were incubated with 0, 50 or 200 ng of PKCz in Rab11 interaction buffer or DLIC interaction buffer for 2 hours at 4 °C. Beads were washed three times with Rab11 interaction buffer supplemented with 50 mM NaCl or DLIC interaction buffer. In both cases, beads were finally washed with Tris HCl 50 mM pH 6.8.

Western blotting was done according to standard procedures using guinea pig anti-Nuf (1:5000), rabbit anti-aPKC (Santa Cruz Biotechnologies, 1:5000), anti-GST (Santa Cruz Biotechnologies, 1:3000), mouse anti-Flag (Sigma, 1:10000), rabbit anti-Kinesin (Cytoskeleton, 1:3000), anti-Glued (Dynactin, 1:1000 [43]), rabbit anti 14-3-3 (Santa Cruz Biotechnologies, 1:500) and mouse anti-α-Tubulin (Sigma, 1:10000).

aPKC phosphorylation assay

This method has been adapted from [44]. For each reaction, 150 ng of recombinant substrate (GST-fusion proteins) was incubated with 140 ng of human recombinant PKCiota or PKCz produced in Sf9 cells (Moscat laboratory) in phosphorylation buffer {(35 mM Tris HCl pH 7.5, 10 mM MgCl2, 0.1 mM CaCl2, 0.5 mM EGTA, 1 mM DTT and 0.1 mM ATPγS (Biolog)}) for 1 hour at 30 °C. The reaction was stopped by adding 20 mM EDTA and samples were incubated with 2.5 mM PNMB (Abcam) for 1 hour at room temperature. The samples were analysed by SDS-PAGE and western blotting using anti-thiophosphate ester antibody (Abcam, 1:5000). For MS/MS detection of Nuf phosphopeptides, an in-vitro kinase assay was performed using 10 μg of GST-Nuf FL and 10 μg of PKCiota in 400uM ATP and 1x phosphorylation buffer. Phosphorylation products were digested and processed using LS-MS/MS analysis by the SBMRI Proteomic Service.

Phosphorylated Nuf −14-3-3ε pull-down assay

A total of 20 μg of GST and GST-NufFL wt were incubated for 1 hour at room temperature with phosphorylation buffer adding ATP and 500 μg of PKCz only to the Nuf phosphorylated experiment. Beads were washed three times with 14-3-3 interaction buffer (25 mM Hepes pH 7.5, 12.5 mM MgCl2, 20 % glycerol, 0.1 % NP40, 150 mM KCl, 1 mM DTT) and were incubated with 500 ng of eluted 14-3-3ε (removing GST with Prescission protease) in 14-3-3 interaction buffer adding 30 μg of BSA for 3 hours at 4 °C. After that, beads were washed three times with 20 mM Tris HCl pH 8, 100 mM NaCl, 1 mM EDTA and 0.5 % NP40, and once with Tris HCl 50 mM pH 6.8.

Additional Methods, including primers used, are given in Additional file 7.

Abbreviations

aPKC: 

Atypical protein kinase C

ARE: 

Apical recycling endosomes

Crb: 

Crumbs

DPATJ: 

Drosophila Pals1 associated tight junctions

E-Cad: 

E-cadherin

FIPs: 

Family interacting proteins

KD: 

Kinase domain

Nuf: 

Nuclear fallout

RD: 

Regulatory domain

RE: 

Recycling endosomes

REdv: 

Recycling endosomes derived vesicles

Declarations

Acknowledgements

We thank B. A. Eaton, T. Hays, T. Schwarz, W. Sullivan, T. Otani, S. Hayashi, M. González-Gaitán for reagents; J. C-G Hombría, J.R. Martínez, J. Culí and S. Campuzano for suggestions to the manuscript. S.S. and, JM. E-V. are supported through grants of the Ministerio de Educación y Ciencia to J. C-G Hombría. This work was supported by grants of the MICINN/FEDER

to J. C-G Hombría and S.S (BFU2010-15020, BFU2010-15851 and BFU2013-45866), grant of the Junta de Andalucía (P11-CVI7256) and by NIH Grants R01CA172025 to J.M., and R01CA192642 to M.T.D.-M. and 5P30CA030199 to M.T.D-M. and J.M.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
CABD, CSIC/JA/UPO, Campus Universidad Pablo de Olavide, Ctra. De Utrera Km. 1, Seville, 41013, Spain
(2)
Sanford-Burnham Medical Research Institute, La Jolla, CA 92037, USA

References

  1. Bryant DM, Mostov KE. From cells to organs: building polarized tissue. Nat Rev Mol Cell Biol. 2008;9(11):887–901.View ArticlePubMedPubMed CentralGoogle Scholar
  2. Rodriguez-Boulan E, Macara IG. Organization and execution of the epithelial polarity programme. Nat Rev Mol Cell Biol. 2014;15(4):225–42.View ArticlePubMedPubMed CentralGoogle Scholar
  3. Boggiano JC, Fehon RG. Growth control by committee: intercellular junctions, cell polarity, and the cytoskeleton regulate Hippo signaling. Dev Cell. 2012;22(4):695–702.View ArticlePubMedPubMed CentralGoogle Scholar
  4. Harris KP, Tepass U. Cdc42 and vesicle trafficking in polarized cells. Traffic. 2010;11(10):1272–9.View ArticlePubMedGoogle Scholar
  5. Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, Martin-Belmonte F, Mostov KE. A molecular network for de novo generation of the apical surface and lumen. Nat Cell Biol. 2010;12(11):1035–45.View ArticlePubMedPubMed CentralGoogle Scholar
  6. de Vreede G, Schoenfeld JD, Windler SL, Morrison H, Lu H, Bilder D. The Scribble module regulates retromer-dependent endocytic trafficking during epithelial polarization. Development. 2014;141(14):2796–802.View ArticlePubMedPubMed CentralGoogle Scholar
  7. Yoshihama Y, Sasaki K, Horikoshi Y, Suzuki A, Ohtsuka T, Hakuno F, et al. KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity in epithelial cells. Curr Biol. 2011;21(8):705–11.View ArticlePubMedGoogle Scholar
  8. Riggs B, Rothwell W, Mische S, Hickson GR, Matheson J, Hays TS, et al. Actin cytoskeleton remodeling during early Drosophila furrow formation requires recycling endosomal components Nuclear-fallout and Rab11. J Cell Biol. 2003;163(1):143–54.View ArticlePubMedPubMed CentralGoogle Scholar
  9. Rosse C, Linch M, Kermorgant S, Cameron AJ, Boeckeler K, Parker PJ. PKC and the control of localized signal dynamics. Nat Rev Mol Cell Biol. 2010;11(2):103–12.View ArticlePubMedGoogle Scholar
  10. Sotillos S, Diaz-Meco MT, Caminero E, Moscat J, Campuzano S. DaPKC-dependent phosphorylation of Crumbs is required for epithelial cell polarity in Drosophila. J Cell Biol. 2004;166(4):549–57.View ArticlePubMedPubMed CentralGoogle Scholar
  11. Otani T, Oshima K, Onishi S, Takeda M, Shinmyozu K, Yonemura S, et al. IKKepsilon regulates cell elongation through recycling endosome shuttling. Dev Cell. 2011;20(2):219–32.View ArticlePubMedGoogle Scholar
  12. Fujise A, Mizuno K, Ueda Y, Osada S, Hirai S, Takayanagi A, et al. Specificity of the high affinity interaction of protein kinase C with a physiological substrate, myristoylated alanine-rich protein kinase C substrate. J Biol Chem. 1994;269(50):31642–8.PubMedGoogle Scholar
  13. Nagai-Tamai Y, Mizuno K, Hirose T, Suzuki A, Ohno S. Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells. Genes Cells. 2002;7(11):1161–71.View ArticlePubMedGoogle Scholar
  14. Riggs B, Fasulo B, Royou A, Mische S, Cao J, Hays TS, et al. The concentration of Nuf, a Rab11 effector, at the microtubule-organizing center is cell cycle regulated, dynein-dependent, and coincides with furrow formation. Mol Biol Cell. 2007;18(9):3313–22.View ArticlePubMedPubMed CentralGoogle Scholar
  15. Tzivion G, Avruch J. 14-3-3 proteins: active cofactors in cellular regulation by serine/threonine phosphorylation. J Biol Chem. 2002;277(5):3061–4.View ArticlePubMedGoogle Scholar
  16. Benton R, St Johnston D. Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells. Cell. 2003;115(6):691–704.View ArticlePubMedGoogle Scholar
  17. Hao Y, Du Q, Chen X, Zheng Z, Balsbaugh JL, Maitra S, et al. Par3 controls epithelial spindle orientation by aPKC-mediated phosphorylation of apical Pins. Curr Biol. 2010;20(20):1809–18.View ArticlePubMedPubMed CentralGoogle Scholar
  18. Hurd TW, Fan S, Liu CJ, Kweon HK, Hakansson K, Margolis B. Phosphorylation-dependent binding of 14-3-3 to the polarity protein Par3 regulates cell polarity in mammalian epithelia. Curr Biol. 2003;13(23):2082–90.View ArticlePubMedGoogle Scholar
  19. Suzuki A, Hirata M, Kamimura K, Maniwa R, Yamanaka T, Mizuno K, et al. aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity. Curr Biol. 2004;14(16):1425–35.View ArticlePubMedGoogle Scholar
  20. Simon GC, Prekeris R. Mechanisms regulating targeting of recycling endosomes to the cleavage furrow during cytokinesis. Biochem Soc Trans. 2008;36(Pt 3):391–4.View ArticlePubMedPubMed CentralGoogle Scholar
  21. Desclozeaux M, Venturato J, Wylie FG, Kay JG, Joseph SR, Le HT, et al. Active Rab11 and functional recycling endosome are required for E-cadherin trafficking and lumen formation during epithelial morphogenesis. Am J Physiol Cell Physiol. 2008;295(2):C545–56.View ArticlePubMedGoogle Scholar
  22. Shaye DD, Casanova J, Llimargas M. Modulation of intracellular trafficking regulates cell intercalation in the Drosophila trachea. Nat Cell Biol. 2008;10(8):964–70.View ArticlePubMedGoogle Scholar
  23. Elias S, McGuire JR, Yu H, Humbert S. Huntingtin Is required for epithelial polarity through RAB11A-mediated apical trafficking of PAR3-aPKC. PLoS Biol. 2015;13(5):e1002142.View ArticlePubMedPubMed CentralGoogle Scholar
  24. Singari S, Javeed N, Tardi NJ, Marada S, Carlson JC, Kirk S, et al. Inducible protein traps with dominant phenotypes for functional analysis of the Drosophila genome. Genetics. 2014;196(1):91–105.View ArticlePubMedPubMed CentralGoogle Scholar
  25. Emery G, Hutterer A, Berdnik D, Mayer B, Wirtz-Peitz F, Gaitan MG, et al. Asymmetric Rab 11 endosomes regulate delta recycling and specify cell fate in the Drosophila nervous system. Cell. 2005;122(5):763–73.View ArticlePubMedGoogle Scholar
  26. Langevin J, Morgan MJ, Sibarita JB, Aresta S, Murthy M, Schwarz T, et al. Drosophila exocyst components Sec5, Sec6, and Sec15 regulate DE-Cadherin trafficking from recycling endosomes to the plasma membrane. Dev Cell. 2005;9(3):365–76.View ArticlePubMedGoogle Scholar
  27. Morais-de-Sa E, Mirouse V, St Johnston D. aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. Cell. 2010;141(3):509–23.View ArticlePubMedPubMed CentralGoogle Scholar
  28. Betschinger J, Mechtler K, Knoblich JA. The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl. Nature. 2003;422(6929):326–30.View ArticlePubMedGoogle Scholar
  29. Gamblin CL, Hardy EJ, Chartier FJ, Bisson N, Laprise P. A bidirectional antagonism between aPKC and Yurt regulates epithelial cell polarity. J Cell Biol. 2014;204(4):487–95.View ArticlePubMedPubMed CentralGoogle Scholar
  30. Hurov JB, Watkins JL, Piwnica-Worms H. Atypical PKC phosphorylates PAR-1 kinases to regulate localization and activity. Curr Biol. 2004;14(8):736–41.View ArticlePubMedGoogle Scholar
  31. Nishimura T, Kaibuchi K. Numb controls integrin endocytosis for directional cell migration with aPKC and PAR-3. Dev Cell. 2007;13(1):15–28.View ArticlePubMedGoogle Scholar
  32. Plant PJ, Fawcett JP, Lin DC, Holdorf AD, Binns K, Kulkarni S, et al. A polarity complex of mPar-6 and atypical PKC binds, phosphorylates and regulates mammalian Lgl. Nat Cell Biol. 2003;5(4):301–8.View ArticlePubMedGoogle Scholar
  33. Smith CA, Lau KM, Rahmani Z, Dho SE, Brothers G, She YM, et al. aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb. EMBO J. 2007;26(2):468–80.View ArticlePubMedPubMed CentralGoogle Scholar
  34. Hyodo-Miura J, Yamamoto TS, Hyodo AC, Iemura S, Kusakabe M, Nishida E, et al. XGAP, an ArfGAP, is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation. Dev Cell. 2006;11(1):69–79.View ArticlePubMedGoogle Scholar
  35. Kusakabe M, Nishida E. The polarity-inducing kinase Par-1 controls Xenopus gastrulation in cooperation with 14-3-3 and aPKC. EMBO J. 2004;23(21):4190–201.View ArticlePubMedPubMed CentralGoogle Scholar
  36. Winter JF, Hopfner S, Korn K, Farnung BO, Bradshaw CR, Marsico G, et al. Caenorhabditis elegans screen reveals role of PAR-5 in RAB-11-recycling endosome positioning and apicobasal cell polarity. Nat Cell Biol. 2012;14(7):666–76.View ArticlePubMedGoogle Scholar
  37. Shandala T, Woodcock JM, Ng Y, Biggs L, Skoulakis EM, Brooks DA, et al. Drosophila 14-3-3epsilon has a crucial role in anti-microbial peptide secretion and innate immunity. J Cell Sci. 2011;124(Pt 13):2165–74.View ArticlePubMedGoogle Scholar
  38. Johnson JL, He J, Ramadass M, Pestonjamasp K, Kiosses WB, Zhang J, et al. Munc13-4 Is a Rab11-binding protein that regulates Rab11-positive vesicle trafficking and docking at the plasma membrane. J Biol Chem. 2016;291(7):3423–38.View ArticlePubMedGoogle Scholar
  39. Rothwell WF, Fogarty P, Field CM, Sullivan W. Nuclear-fallout, a Drosophila protein that cycles from the cytoplasm to the centrosomes, regulates cortical microfilament organization. Development. 1998;125(7):1295–303.PubMedGoogle Scholar
  40. Beronja S, Laprise P, Papoulas O, Pellikka M, Sisson J, Tepass U. Essential function of Drosophila Sec6 in apical exocytosis of epithelial photoreceptor cells. J Cell Biol. 2005;169(4):635–46.View ArticlePubMedPubMed CentralGoogle Scholar
  41. McGrail M, Gepner J, Silvanovich A, Ludmann S, Serr M, Hays TS. Regulation of cytoplasmic dynein function in vivo by the Drosophila Glued complex. J Cell Biol. 1995;131(2):411–25.View ArticlePubMedGoogle Scholar
  42. Simoes Sde M, Blankenship JT, Weitz O, Farrell DL, Tamada M, Fernandez-Gonzalez R, et al. Rho-kinase directs Bazooka/Par-3 planar polarity during Drosophila axis elongation. Dev Cell. 2010;19(3):377–88.View ArticlePubMedGoogle Scholar
  43. Chang L, Kreko T, Davison H, Cusmano T, Wu Y, Rothenfluh A, et al. Normal dynactin complex function during synapse growth in Drosophila requires membrane binding by Arfaptin. Mol Biol Cell. 2013;24(11):1749–64. S1741-1745.View ArticlePubMedPubMed CentralGoogle Scholar
  44. Allen JJ, Li M, Brinkworth CS, Paulson JL, Wang D, Hubner A, et al. A semisynthetic epitope for kinase substrates. Nat Methods. 2007;4(6):511–6.View ArticlePubMedPubMed CentralGoogle Scholar

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