Role of PCNA and RFC in promoting Mus81-complex activity
- Alexandra Sisakova†1, 2, 3,
- Veronika Altmannova†1, 3,
- Marek Sebesta1, 2, 4 and
- Lumir Krejci1, 2, 3Email authorView ORCID ID profile
© Krejci et al. 2017
Received: 11 June 2017
Accepted: 15 September 2017
Published: 2 October 2017
Proper DNA replication is essential for faithful transmission of the genome. However, replication stress has serious impact on the integrity of the cell, leading to stalling or collapse of replication forks, and has been determined as a driving force of carcinogenesis. Mus81-Mms4 complex is a structure-specific endonuclease previously shown to be involved in processing of aberrant replication intermediates and promotes POLD3-dependent DNA synthesis via break-induced replication. However, how replication components might be involved in this process is not known.
Herein, we show the interaction and robust stimulation of Mus81-Mms4 nuclease activity by heteropentameric replication factor C (RFC) complex, the processivity factor of replicative DNA polymerases that is responsible for loading of proliferating cell nuclear antigen (PCNA) during DNA replication and repair. This stimulation is enhanced by RFC-dependent ATP hydrolysis and by PCNA loading on the DNA. Moreover, this stimulation is not specific to Rfc1, the largest of subunit of this complex, thus indicating that alternative clamp loaders may also play a role in the stimulation. We also observed a targeting of Mus81 by RFC to the nick-containing DNA substrate and we provide further evidence that indicates cooperation between Mus81 and the RFC complex in the repair of DNA lesions generated by various DNA-damaging agents.
Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
KeywordsReplication factor C Proliferating cell nuclear antigen Mus81 complex Replication Recombination
During DNA replication, endogenous and exogenous factors can cause replication forks (RFs) to stall and/or collapse, a condition commonly known as replication stress [1, 2]. Common fragile sites are particularly unstable under replication stress; they are DNA sequences, scattered throughout the genome, that are intrinsically difficult to replicate due to their inherent propensity to form secondary structures, causing RF stalling. These events may be associated with occurrence of ssDNA gaps and/or DNA breaks [2, 3]. Correct progression and termination of DNA replication is essential to maintain genome integrity of the cell , since genomic instability resulting from replication stress has been recognized as a driver in carcinogenesis . Cells have evolved multiple pathways to restart stalled RFs, with one of the mechanisms involved being homologous recombination through stabilization of RFs and invasion into homologous sequences found within the sister chromatid, resulting in a transient formation of a sister-chromatid junction . However, prolonged accumulation of these intermediate structures is toxic for the cell. Therefore, homologous recombination requires the action of the Sgs1-Top3-Rmi1 complex, which catalyzes dissolution of DNA intermediates, yielding exclusively non-crossover products and thereby suppressing crossover formation . Alternatively, a battery of structure-specific endonucleases (Yen1, Slx1-Slx4, Mus81-Mms4) can cleave the joint DNA structures .
The Mus81-Mms4 complex (henceforth named the Mus81 complex) is a highly conserved, structure-specific endonuclease. It is a member of the XPF family of proteins that plays a crucial role in maintaining genome integrity in various DNA processing pathways . This complex is implicated in resolution of recombination intermediates, in inter-strand DNA cross-link repair, and in the recovery of stalled or blocked RFs . In vitro, the Mus81 complex shows preference for substrates mimicking RFs, namely 3′ flap structures, nicked Holliday junctions, and D-loop structures (mimicking sister-chromatid junctions occurring in vivo) . Recent studies have demonstrated that the activity of the Mus81 complex is regulated during the cell cycle by Cdk1 and Cdc5 kinases, as well as via protein-mediated interactions [10–12]. Interestingly, a recent study in yeast revealed another cell cycle kinase, Cdc7-Dbf4, which binds and phosphorylates the Mus81 complex in mitosis, and in association with Cdc5 is required for full Mus81 complex activation . The activation of the complex occurs by phosphorylation of the Mms4 subunit at G2/M transition to assure the resolution of joint DNA structures that persist after the bulk DNA synthesis has occurred but prior to chromosome segregation. At the same time, post-replicative activation of the Mus81 complex limits potentially undesirable cleavage of normal RFs during S phase that may lead to double-strand break (DSB) formation [10, 14]. Accordingly, WEE1 in human cells was shown to restrain CDK1-mediated assembly of MUS81-SLX4 in S phase, which is required for efficient substrate targeting . In addition, several factors involved in DNA replication and repair (in yeast: Rad27, Rad54, Srs2, Esc2, Rad52; in mammalian cells: FEN1, BLM, RAD54, RAD52) have been shown to stimulate nuclease activity of the Mus81 complex [16–20]. All these data clearly indicate the need for tight control and regulation of Mus81 complex activity.
Human MUS81-EME1 promotes replication restart by converting collapsed RFs into DNA DSBs upon replication inhibition [21, 22]. Moreover, a recent study revealed that the human MUS81 complex plays an important role in common fragile site expression, occurring in the early mitotic phase of the cell cycle. Under these circumstances, activity of the MUS81 complex promotes POLD3 (human orthologue of yeast Pol32)-dependent DNA synthesis via the break-induced replication (BIR) mechanism . Fittingly, yeast cells defective in either subunit of the nuclease complex exhibit sensitivity to agents causing RF stalling such as camptothecin (CPT), hydroxyurea (HU), and methyl methanesulfonate (MMS) . Furthermore, Mus81 plays an essential role in limiting mutagenicity during BIR, which can be highly inaccurate and prone to switching templates , by cleavage of unstable D-loop structure and thus stabilizing the fork structure . An active role of the MUS81 complex in timely replication has also been suggested, as MUS81-deficient cells fail to recover from replication inhibitors, and viability of MUS81-defiecient cells depends on XPF nuclease . Yeast MUS81 and MMS4 have been shown to genetically interact with several DNA replication factors. In S. pombe, the Mus81 complex collaborates with Rad2, a FEN1 and Rad27 ortholog, during Okazaki fragment maturation , further corroborating the notion that the Mus81 complex is required for completion of DNA replication.
Proliferating cell nuclear antigen (PCNA) is the key component of replication machinery. It functions as a trimeric complex that encircles DNA, serving as a processivity factor of DNA polymerases, historically known as the sliding clamp. For its proper activity, it must be actively loaded onto DNA. This process requires a clamp loader, replication factor C (RFC), which loads PCNA onto primer-template junctions in an ATP-dependent manner [29, 30]. RFC is a heteropentameric complex composed of a large Rfc1 subunit (94.9 kDa) and four small subunits – Rfc2 (39.7 kDa), Rfc3 (38.2 kDa), Rfc4 (36 kDa), and Rfc5 (40 kDa). All five subunits contain highly conserved regions known as RFC boxes (II–VIII), which are essential for cell viability . Importantly, certain mutations in RFC2, RFC4, and RFC5 exhibit sensitivity to HU and MMS , indicating that active PCNA loading may play a role in DNA repair. There are three paralogs of the RFC1 gene present in eukaryotes, CTF18, RAD24, and ELG1, which form alternative, RFC-like complexes (RLCs) with the smaller subunits (Rfc2–5) [33–36].
In this study, we investigated the possibility that the RFC and Mus81 complexes cooperate under conditions of replication stress in resolution of stalled RFs and branched DNA structures emanating from the restarted RFs. We first established that these two complexes interact in vitro and in vivo. Next, we studied the functional consequence of the newly described interaction. We found that the RFC complex robustly stimulates the endonuclease activity of the Mus81 complex in vitro. Moreover, this stimulation is further enhanced by ATPase activity of the RFC complex, and also by the PCNA loading on the DNA substrate. We also demonstrate that this stimulation is not specific to the Rfc1 subunit, as other RLCs (Elg1-RLC) can stimulate the activity of the Mus81 complex. We further corroborated our in vitro observation by analyzing the genetic interaction between a diverse set of mutant RFC alleles and MUS81. We found an epistatic relationship between these two factors in presence of MMS, indicating cooperation of RFC and Mus81 complex in repair of lesions under this condition. Interestingly, the sensitivity of RFC mutants to cis-platin (CisPT) and zeocin (ZEO) was suppressed in ∆mus81 background, signifying toxic intermediates might be generated by the Mus81 complex in this mutant. Our data point to the possibility that, under particular instances of replicative stress, the activity of the Mus81 complex can be promoted by a component of a replisome, the RFC complex.
PCNA interaction with the Mus81 complex and its effect on the Mus81 complex nuclease activity
Taken together, PCNA interacts specifically with the Mus81 subunit of the Mus81 complex, and this interaction is mediated by the PIP box motif and promotes the nuclease activity of the Mus81 complex on the variety of DNA substrates.
Mus81 complex interacts with RFC complex
RFC stimulates Mus81-Mms4 and Rad27 nuclease activities
To determine whether this stimulation is species-specific, we performed an experiment with human MUS81-EME1 complex. As shown in Additional file 3: Figure S2A, the yeast RFC complex was also able to stimulate the activity of the human MUS81 complex, indicating that the stimulation mechanism is general and not species-specific.
Similarly as for PCNA, we also analyzed the effect of RFC complex on Mus81 nuclease activity on fork structures and nicked Holliday junctions. Expectedly, we observed substantial stimulation of the Mus81 complex enzymatic activity on both substrates in the level comparable to 3′ flap DNA (Additional file 3: Figure S2B–G; Additional file 2).
The Mus81 complex and Rad27 are stimulated by Elg1-RLC
To date, three alternative RFC-like complexes have been described, the Ctf18-RLC, Rad24-RLC, and Elg1-RLC, respectively. To determine whether the stimulation of the Mus81 complex activity by RFC is specific to the canonical one, we decided to test the effect of an alternative RFC-like complex in which the Rfc1 subunit is replaced by the Elg1 protein. To test the ability of Elg1-RLC to stimulate the Mus81 nuclease activity, we performed an experiment similar to that described above (Fig. 4). The Mus81 complex and Rad27 (both at 0.25 nM) were incubated with increasing amounts of Elg1-RLC (0.25–10 nM). Similarly as with the RFC complex, we observed robust stimulation of both the Mus81 complex and Rad27 by Elg1-RLC complex (Fig. 4g, h; Additional file 2). Specifically, at 2.5 nM Elg1-RLC we observed 7- and 13-fold stimulation of the nuclease activity of Rad27 and the Mus81 complex, respectively.
Effect of both RFC and PCNA on activity of the Mus81 complex
Since RFC and PCNA form a functional complex during DNA replication and repair, we also investigated the effect of the simultaneous presence of PCNA and RFC on the enzymatic activity of the Mus81 complex. We found that the addition of both RFC (0.25–2.5 nM) and PCNA in the reaction did not increase the quantity of cleaved product compared to the reactions with RFC alone (Additional file 1; Additional file 4: Figure S3A, B).
Epistatic relationship of interaction between the Mus81 complex and the RFC complex
Moreover, rfc4-K55R exhibited an increased sensitivity to high concentrations of HU in spot and cell survival assays, respectively (Fig. 6a and Additional file 7: Figure S5). Interestingly, rfc4-K55R mus81 double mutant cells were as or even more sensitive to HU than either single mutant, which could potentially indicate a separate role for the two factors in dealing with stalled RFs caused by depletion of dNTP pools. However, colony counts, where the rfc4-K55R mus81 double mutant showed statistically significant difference on HU plates compared to wild-type (P < 0.05), revealed a growth defect of this mutant, which probably results in a high sensitivity in the spot assay. Noteworthy, using survival assays in liquid media, we did not observe additional sensitivity of rfc4-K55R mus81 double mutants. Nevertheless, this could be due to lower sensitization by HU in this experiment.
We also analyzed the effect of CisPT and ZEO using a spot assay, which revealed the highest sensitivity of the rfc4-K55R single mutant. Interestingly, this was suppressed by deletion of MUS81, which might suggest that the Mus81 complex may generate toxic intermediates in this RFC mutant.
The Mus81 complex plays an important role in the maintenance of genome integrity under conditions of replication stress via processing stalled and/or blocked RFs, and in reinitiating the replication in early mitosis [21–23]. Although the role of the Mus81 complex has recently been studied intensively, it is not yet fully understood how this complex is targeted on sites of actions, nor has it been fully described how components of replication machinery might affect the activity of the Mus81 complex.
In this study, we report on a robust stimulation of the activity of the Mus81 complex by yeast RFC. Moreover, this stimulation is further enhanced by ATPase activity of the RFC complex, by the PCNA loading on the DNA substrate, and by targeting Mus81 to a nick-containing substrate. The last being in agreement with the binding distribution of RFC/PCNA on various substrates . Furthermore, we detected direct physical interaction between the Mus81 complex and PCNA, and showed that the interaction is mediated by the PIP box. In addition, the experiment with mutant forms of PCNA revealed that the IDCL domain on PCNA is responsible for Mus81 binding. This domain serves as a pocket for the hydrophobic residues of PIP box motif, further supporting the role of the PIP box motif on Mus81p in PCNA binding. Further biochemical analysis nevertheless suggested that the interaction with PCNA might play a minor role in recruiting and/or stabilizing the Mus81 complex onto the DNA substrate, in particular when loaded on the DNA substrate. Indeed, PCNA is known to bind a variety of proteins to ensure their presence at the active centers of DNA replication and repair . It targets Exo1 to the ssDNA gaps left behind the RF . Similarly, Rad27 was shown to interact with PCNA . Moreover, mutations within the PIP box of FEN1, the human orthologue of Rad27, abolished FEN1 recruitment to RF, which is associated with severe defects in replication progression and may lead to carcinogenesis . Since FEN1 is stimulated by the human RFC complex , we compared the effect of RFC on both the Mus81 complex and Rad27. We observed that the activity of Rad27 is robustly stimulated by the RFC complex, confirming the preservation and importance of this stimulation between species. Interestingly, the enzymatic activity of the Mus81 complex was stimulated by the RFC complex to an even higher extent compared to Rad27 stimulation. Moreover, human MUS81-EME1 was also stimulated by yeast RFC, thus confirming that the stimulation is not species-specific.
The fate of the RFC complex after PCNA loading onto a primer-template junction is, at present, unclear. Several studies suggest that RFC dissociates from DNA once PCNA is loaded onto the primer-template junction . However, contradictory evidence suggests that the RFC complex travels along the DNA as part of replication machinery . The second scenario is supported by our findings that the RFC complex effectively stimulates the Mus81 complex. As it moves along the DNA substrate during replication, it would be available to stimulate the Mus81 complex nuclease activity. Consistent with this proposed explanation, we demonstrate the interaction between Mus81 and the RFC complex in an in vivo pull-down experiment. Further experiments with a truncated form of Mus81 (1–319) revealed that the interaction site is situated at the N-terminus of Mus81. Although it cannot be excluded that other factor(s) may mediate this interaction, we assume a direct, albeit transient, interaction between the Mus81 complex and RFC. PCNA could play a role as a mediator of this interaction as the N-terminus of Mus81 also contains a PIP box motif (Fig. 1a).
In the cells, three additional complexes (termed RFC-like complexes in which the largest subunit is exchanged for either Ctf18, Rad24, or Elg1) exist. We wondered whether these complexes could also play a role in activation of the Mus81 complex. Here, we also show that the robust stimulation of the Mus81 complex is not specific only to the RFC complex, since the Elg1-RFC-like complex was also able to stimulate nuclease activity of the Mus81 complex. Although we cannot exclude the possibility that both Rfc1 and Elg1 contain the Mus81 interaction motif, since all subunits of the RFC complex are highly homologous in certain amino acid sequences called RFC boxes (box II–VII), we think that the stimulatory motif might be conserved within all subunits of this complex. Fittingly, a short motif localized in box VII of human RFC subunits has been found to be responsible for the stimulation of human FEN1 . It is therefore possible that alternative clamp loaders/unloaders also play a role in targeting and/or stabilizing the Mus81 complex onto the DNA and stimulating its activity. Interestingly, Bellaoui et al.  demonstrated synthetic lethality between Mus81 and Elg1, indicating that Elg1 may represent alternative targeting complex for Mus81. This notion may be also supported by synthetic lethality observed between Elg1 and RFC subunits .
The relevance of the interaction between the RFC complex and the Mus81 complex was also verified in vivo using various assays. Single and double mutant strains were tested for sensitivity to different DNA-damaging drugs. We observed a comparable growth defect of the double rfc4-K55R mus81 mutant and a single mutant carrying the deletion of MUS81 when the cells were exposed to CPT and to some extent also to MMS. These data might suggest that RFC and the Mus81 complex are involved in the same pathway as well as their possible cooperation in resolution of recombination intermediates and DNA lesions caused by these drugs. Interestingly, the sensitivity of rfc4-K55R mutant to CisPT and ZEO was suppressed by deletion of MUS81, which might indicate that the Mus81 complex generates toxic intermediates in this RFC mutant. On the other hand, both mutations resulted in a synergistic phenotype after the cells were treated with HU, indicating separate roles of Mus81 and the RFC complex under these conditions. This result is supported by a study showing that the Mus81 complex is not necessary for DNA replication resumption after HU treatment ; however, it could act independently of RFC in the later steps of repair.
In summary, we provide evidence that the Mus81 complex, as an important factor involved in resolution of stalled and/or blocked RFs, is targeted and stabilized in the place of its action by the PCNA/RFC complex. We believe our data help shed light onto the complex interplay of different repair mechanisms that take place during DNA replication. Additionally, it has been shown that replicative stress plays a key role in tumorigenesis ; our results point to new options for control of the repair mechanisms and the subsequent treatment of disorders associated with genome instability.
Yeast strains and plasmids
The S. cerevisiae strains and plasmids used in this study are listed in Additional file 9: Table S1. All strains used for drug sensitivity assays are derivatives of W1588-4C (MATa ade2-1 can1-100 ura3-1 his3-11, 15 leu2-3,112 trp1-1) , a RAD5 derivative of W303-1A . Standard genetic techniques were used for manipulating yeast strains . The Δmus81 strain was created by replacing the gene coding sequence with the HIS5-disruption cassette in the wild-type strain. The correct integration was verified by PCR reaction. The yeast strains rfc4-K55R and rfc4-K55E were generated by transformation of plasmids pBL625 or pBL633-E into the strain PY94 (generous gifts from Dr. Peter Burgers). The mutants were selected on plates with 5-fluoroorotic acid. MATα rfc4 mutant strains were prepared by crossing wild-type and MATa rfc4-K55R or rfc4-K55E. Crossing haploid Δmus81 and the particular rfc4 single mutant strains generated corresponding double mutant strains. The heterozygous diploids were sporulated and the tetrads were dissected. Double mutant haploid strains were subsequently verified using PCR and selected for specific markers.
The plasmid for expression of Mus81 (1–319) E44Q mutant was prepared by site-directed mutagenesis using specific primers (Additional file 10: Table S2).
Expression and purification of Mus81-Mms4 (MUS81-EME1)
The yeast Mus81-Mms4 and human MUS81-EME1 expression plasmids were a generous gift from Dr. Matthew Whitby (University of Oxford) and Dr. Steve West (The Francis Crick Institute), respectively. Expression and purification of these complexes has been described elsewhere . Briefly, lysate was prepared from 40 g of E. coli cell paste using sonication in 200 mL of lysis buffer C (50 mM Tris-HCl (pH 7.5), 10% sucrose, 10 mM EDTA, 1 mM β-mercaptoethanol, 0.01% Nonidet P-40, and protease inhibitors (aprotinin, chymostatin, leupeptin, pepstatin A, benzamidine, each at 5 μg/mL)) containing 150 mM KCl. Cleared lysate was applied sequentially onto a 20-mL Q-Sepharose and a 20-mL SP-Sepharose column (GE Healthcare). Proteins were eluted from the SP-Sepharose column with a 200-mL gradient from 150 to 1000 mM KCl in buffer K (20 mM K2HPO4 (pH 7.5), 10% sucrose, 10 mM EDTA, 1 mM β-mercaptoethanol, and 0.01% Nonidet P-40). The peak fractions were pooled and mixed with 1 mL of His-Select nickel affinity gel (Sigma-Aldrich) for 1 h at 4 °C. The beads were washed with 10 mL of buffer K containing 150 mM KCl and 10 mM imidazole. The bound proteins were eluted using 50, 150, 300, and 500 mM imidazole in buffer K containing 150 mM KCl. The 150, 300, and 500 mM imidazole fractions were pooled and loaded onto a 1-mL Heparin column (GE Healthcare). Bound proteins were eluted with a 10-mL gradient from 150 to 1000 mM KCl in buffer K. Peak fractions were pooled, loaded onto a 1-mL Mono S column (GE Healthcare), and eluted with a 10-mL gradient from 150 to 1000 mM KCl in buffer K. Pooled fractions were concentrated, flash-frozen in 5 μL aliquots, and stored at −80 °C.
Expression and purification of Mus81 (1–319)
The E. coli expression construct of Mus81 (1–319) was a kind gift from Dr. Steven Brill (Rutgers University). The expression and purification of Mus81 (1–319) was based on a procedure previously described . Extract from 10 g of cell paste was prepared by sonication in 50 mL of lysis buffer C containing 150 mM KCl and clarified by centrifugation. The supernatant was passed through a 7-mL Q-Sepharose column and the flow-through was directly applied onto a 7-mL SP-Sepharose. Proteins were eluted with a 70-mL gradient from 150 to 1000 mM KCl in buffer K. Mus81 (1–319) peak fractions were pooled and mixed with His-Select nickel affinity gel (Qiagen) for 1 h at 4 °C. The bound proteins were eluted using 500 μL of each 150, 300, and 500 mM imidazole in buffer K containing 150 mM KCl. Peak fractions were again pooled and loaded onto a 1-mL Mono S column. Mus81 (1–319) was eluted using 10-mL gradient from 150 to 1000 mM KCl in buffer K. The fractions containing nearly homogeneous Mus81 (1–319) were concentrated, flash-frozen, and stored in small portions at −80 °C.
Expression and purification of Mus81 (1–319) E44Q
The plasmid expressing Mus81 (1–319) mutant was transformed into E. coli BL21(DE3) strain and protein expression was induced with 0.1 mM IPTG followed by incubation at 16 °C overnight. The cell pellet (8 g) was resuspended in 30 mL of lysis buffer C containing 150 mM KCl. After sonication, the cell lysate was clarified by ultracentrifugation. The supernatant was incubated with 1 mL of His-Select nickel affinity gel (Sigma-Aldrich) for 1 h at 4 °C. The beads were washed with 10 mL of buffer K containing 150 mM KCl. The bound proteins were eluted using 50, 150, 300, 500, and 1000 mM imidazole in buffer K containing 50 mM KCl. Peak fractions were pooled and applied onto a 1.5-mL Source S column. The protein was eluted with a 12-mL gradient from 250 to 1000 mM KCl in buffer K. Fractions containing Mus81 protein were concentrated, flash-frozen, and stored in small portions at −80 °C.
Expression and purification of PCNA, PCNA-79, and PCNA-90
The E. coli strain BL21(DE3), transformed with expression plasmid containing untagged PCNA, PCNA-79, or PCNA-90 (a kind gift from Dr. Peter Burgers, Washington University) was induced with 0.5 mM IPTG for 3 h at 37 °C. Cells were harvested by centrifugation and stored at −80 °C. Wild-type and mutant forms of PCNA were purified as described elsewhere . Briefly, 5 g of E. coli cell paste were sonicated in 30 mL lysis buffer C containing 750 mM KCl. The lysate was clarified by ultracentrifugation (100,000 × g for 1 h at 4 °C) and subjected to ammonium sulfate precipitation at 0.21 g/mL. After stirring for 1 h and centrifugation at 15,000 × g for 20 min at 4 °C, another 0.32 g/mL of ammonium sulfate was added to the supernatant. The resulting precipitate was dissolved in 50 mL of buffer K and the mixture was applied onto a 7-mL Q-Sepharose column. Proteins were eluted with a 70-mL linear gradient from 50 to 1000 mM KCl in buffer K. The fractions containing PCNA were pooled and loaded onto a 1-mL hydroxyapatite column (BioRad) and proteins were eluted with a 10-mL gradient from 50 to 1000 mM K2HPO4 in buffer K. Peak fractions were pooled and loaded onto a 1 mL Mono Q column (GE Healthcare) and proteins were eluted with a 10-mL gradient from 50 to 1000 mM KCl in buffer K. Fractions with purified PCNA were pooled and concentrated in a Vivaspin micro concentrator. Small aliquots (5 μL) were stored at −80 °C.
Expression and purification of RFC complexes
The expression constructs for RFC1 and ELG1 complexes were a kind gift from Dr. Jeff Finkelstein (Rockefeller University) and Dr. Peter Burgers (Washington University), respectively, and they were expressed accordingly . For RFC complex purification, 10 g of cell pellet was lysed in a CryoMill (Retsch) and the resulting powder was resuspended in 50 mL lysis buffer C containing 100 mM KCl, followed by sonication. The lysate was clarified by ultracentrifugation (100,000 × g for 1 h at 4 °C) and applied to a 7-mL SP-Sepharose column. The bound proteins were eluted with a 70-mL linear gradient from 100 to 1000 mM KCl in buffer K. The peak fractions were pooled and loaded onto a 1-mL Mono Q column (GE Healthcare) and the proteins were eluted with a 10-mL gradient from 100 to 900 mM KCl in buffer K. The nearly homogeneous RFC complex was concentrated and stored in small portions at −80 °C.
Elg1-RLC was purified according to the protocol , with minor modifications. The S. cerevisiae BJ5464 strain was transformed with plasmids pBL448 (encoding GST-ELG1 under control of GAL1 promoter) and pBL422 (encoding RFC2, RFC3, RFC4, and RFC5 under control of GAL1 promoter). These materials were generously provided by Dr. Peter Burgers. To overexpress Elg1-RLC, an overnight yeast culture grown in synthetic medium lacking tryptophan and uracil was diluted eight-fold with fresh synthetic medium lacking tryptophan and uracil but containing galactose (2% w/v), glycerol (3%), and lactic acid (3%). The culture was incubated for 22–24 h at 30 °C with shaking. Cells were harvested by centrifugation and stored at −80 °C.
Elg1-RLC was purified by opening up 70–100 g of yeast paste by cryogenic milling. The resulting powder was dissolved in 200 mL of lysis buffer C1 (50 mM Tris-HCl (pH 7.5), 10% sucrose (w/v), 10 mM EDTA, 3 mM dithiothreitol, 0.01% Nonidet P-40, 150 mM NaCl, and the protease inhibitors) and the volume of the crude lysate was measured. Solid ammonium sulfate was then added to the lysate to a final concentration of 300 mM and the mixture was stirred for 25 min at 4 °C. Afterwards, 45 μL of 10% Polymin P per 1 mL of the lysate was added, and the mixture was gently stirred at 4 °C for 10 min. The crude lysate was clarified by centrifugation (100,000 × g for 90 min). Next, the Elg1-RLC complex was precipitated from the cleared lysate using ammonium sulfate (0.35 g/mL), and the precipitate was held overnight at −80 °C. The next day, the precipitate was dissolved in buffer T (25 mM Tris-HCl, 10% (v/v) glycerol, 5 mM EDTA, pH 7.5). The conductivity of the sample was then checked to be equal to that of buffer T containing 150 mM NaCl and mixed with 2 mL of glutathione sepharose (GE Healthcare) equilibrated in the same buffer. After 2–3 h at 4 °C, the beads were washed with 150 mL of buffer T containing 150 mM NaCl. GST-Elg1-RLC was eluted with buffer T containing 150 mM NaCl, supplemented with 20 mM glutathione (reduced form) and 0.05% ampholytes. Fractions containing the complex were pooled and incubated for 3 h at 4 °C with 5–15 μg of PreScission protease (GE Healthcare). The Elg1-RLC complex was then loaded onto a 1-mL Mono S column equilibrated with buffer T containing 100 mM NaCl and 0.05% ampholytes. After a 10-mL wash with buffer T containing 100 mM NaCl, 0.05% ampholytes, 5 mM MgCl2, and 100 μM ATP, the complex was eluted with a 20-mL linear gradient from 100 to 500 mM NaCl. Fractions containing Elg1-RLC were pooled, concentrated in a Vivaspin concentrator, then stored in 2 μL aliquots at −80 °C.
Expression and purification of other proteins
The DNA substrates used in this study were prepared using the synthetic oligonucleotides listed in Additional file 10: Table S2. All oligonucleotides were purchased from VBC Biotech. Oligo1 is fluorescently labeled at the 5′ end. The substrates were prepared as described elsewhere . Briefly, equimolar amounts of individual oligonucleotides were annealed in hybridization buffer H (50 mM Tris–HCl (pH 7.5), 100 mM NaCl, and 10 mM MgCl2). The mixture was heated to 90 °C for 3 min and then cooled down slowly to room temperature. The annealed DNA substrates were purified by fractionation on a 1-mL Mono Q column (GE Healthcare) with a 20-mL gradient of 50 to 1000 mM NaCl in 10 mM Tris–HCl (pH 7.5). Peak fractions were filtered, dialyzed into 50 mM Tris–HCl (pH 7.5), containing 5 mM MgCl2, then concentrated in a Vivaspin concentrator with a 5 kDa cutoff. The concentration of the DNA substrates was determined by absorbance measurement at 260 nm.
Nuclease assays were performed as previously described . Briefly, reactions containing purified Mus81 complex were performed with 5 nM DNA substrate in buffer N (20 mM Tris–HCl (pH 7.5), 0.2 mM DTT, 10 mM MgCl2, and 5% glycerol). For the human MUS81-EME1 complex, the buffer used contained 50 mM Tris–HCl (pH 7.5), 5 mM MgCl2, 1 mM DTT, and 100 μg/mL BSA. After incubation for 20 min at 37 °C, the reaction was stopped by addition of 0.1% SDS and 500 μg/mL of proteinase K and additional incubation for 5 min at 37 °C. The reactions were mixed with 1/10 volume of loading buffer (60% glycerol, 10 mM Tris-HCl (pH 7.4), 60 mM EDTA, and 0.1% Orange G). The fluorescent DNA was visualized by FLA-9000 (Fuji) and quantified using Multi-Gauge V3.2 (Fuji) software. Rad27 reactions were performed as described above, except that the buffer contained 50 mM Tris–HCl (pH 7.5), 1 mM DTT, and 5 mM MgCl2.
In the Mus81 complex targeting assay, 4 nM 3′ flap substrate and 0.25 nM ΦX174 virion circular ssDNA were mixed in N buffer together with 0.4 nM Mus81-Mms4 and increasing concentrations of RFC (5, 12.5, 25, 50 nM). The mixture was incubated at 37 °C for 30 min and analyzed as described above.
Nuclease assay (with circular 3′ flap)
RFC (180 nM) was pre-incubated with circular 3′ flap DNA substrate (6 nM) and increasing concentrations of PCNApka (70, 140, 270 nM) in the presence or absence of ATP (1 mM) in buffer REP (20 mM Tris–HCl pH 7.5, 1 mM DTT, 12 mM MgCl2, 100 mM KCl) at 30 °C for 10 min. Then, Mus81-Mms4 (40 nM) was added to the reactions followed by incubation at 37 °C for 30 min. The reaction was stopped by addition of 0.05% SDS and 0.5 μg/μL proteinase K and additional incubation at 37 °C for 10 min. The reactions were mixed with a loading dye (45% formamide, 0.025% Orange G), boiled for 3 min at 95 °C, and analyzed on 12% acrylamide denaturing gel. The fluorescent DNA was visualized and analyzed as described above.
PCNA loading assay
Phosphorylated PCNApka was prepared as described previously . PCNA loading was analyzed on a circular 3′ flap substrate (6 nM, created by annealing of ΦX174 virion circular ssDNA with pR3276 and fluorescently labeled pR3277) in buffer REP. RFC (180 nM) was incubated with 32P-PCNApka (140/270 nM) in the presence or absence of ATP (1 mM) at 30 °C for 10 min. Then, 0.02% glutaraldehyde was added to the reactions followed by additional incubation at 37 °C for 10 min. The reaction was stopped by addition of loading dye (60% glycerol, 10 mM Tris-HCl pH 7.4, 60 mM EDTA, and 0.025% orange G) and resolved on 0.9% agarose gel in 0.5× TBE buffer. After electrophoresis, the gel was dried on Grade 3 CHR paper (Whatman), exposed to a phosphoimager screen, and scanned using Fuji FLA 9000 imager.
The Mus81 complex was captured using Ni-NTA beads (Bio-Rad) specific for His6-tag on Mms4 protein. The purified Mus81 complex (10 μg) was incubated with yeast extract (150 μL) containing FLAG-tagged Rfc2 and Rfc4, respectively. The reactions were mixed with 20 μL of Ni-NTA beads for 30 min at 4 °C. The supernatant was then removed and the beads were washed twice with 150 μL of IP150 buffer (50 mM Tris–HCl (pH 7.5), 150 mM KCl, 0.5% Triton X-100, and protease inhibitors). The bound proteins were eluted with 30 μL of 5% SDS. Supernatant and bead fractions (10 μL of each) were analyzed on SDS-PAGE followed by immunoblotting using anti-FLAG (1:6000 dilution, Sigma-Aldrich Cat# F3165 RRID:AB_259529) and anti-His antibodies (1:8000 dilution, Sigma-Aldrich Cat# H1029 RRID:AB_260015), respectively, and secondary anti-mouse antibodies (1:40000 dilution, Sigma-Aldrich Cat# A0168 RRID:AB_257867).
PCNA or BSA was conjugated with Affi-beads (Bio-Rad) according to the manufacturer’s protocol. Purified Mus81 complex was mixed with 10 μL of beads in 30 μL of Tris buffer containing indicated concentration of KCl and the reaction was incubated for 30 min at 4 °C. The beads were washed twice with reaction buffer and bound proteins were eluted with 30 μL of 5% SDS.
Binding affinity quantifications via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The purified PCNA protein was labeled using the L001 Monolith NT.115 protein labeling kit RED-NHS (Amine Reactive) dye. Affinity measurements were performed using MST buffer (T buffer containing 0.05% Tween-20 and 150 mM KCl). Samples were soaked into NT.115 standard treated capillaries (MO-K002, NanoTemper Technologies). Measurements were performed at 25 °C, 70% LED, 40% IR laser power, constant concentration of labeled PCNA (22 nM) and increasing concentration of Mus81 fragment (1.3–5500 nM). Data were analyzed by the MO.Affinity Analysis software (NanoTemper Technologies).
For spot analysis, yeast strains were inoculated in YPD liquid medium and grown overnight at 30 °C. The following day, the overnight culture was diluted to OD600 = 0.2 and grown for an additional 3 h. The concentration of each culture was adjusted to OD600 = 0.2 and serial dilutions were performed in order to reach a corresponding cell density (1 × 105, 1 × 104, 1 × 103, 1 × 102, 1 × 101 cell/mL). From each of these dilutions, 3 μL were spotted onto YPD plates containing HU, CPT, MMS, CisPT, or ZEO, respectively, at the indicated concentrations. Images were taken after 2 days of incubation at 30 °C.
For colony formation assay, the diluted cells were spread onto YPAD plates containing selected drugs and the colonies were counted after 2–4 days of incubation at 30 °C. Three repeats were performed and the means and standard deviations were calculated. The statistical analysis was performed using a two-tailed unpaired homoscedastic t-test.
Cell survival assay
For analysis in liquid medium, the yeast strains were inoculated in 3 mL YPAD at 30 °C overnight. The next day, cultures were diluted to OD600 0.1 and grown for an additional 2.5 h at 30 °C. Then, the selected drugs (MMS, CPT and HU) were added to each culture and growth was monitored by OD600 measurement at specific time points. Three biological replicates were performed for each strain. For quantification, the OD600 of each culture was normalized to the OD600 value of the untreated culture at the same time point.
We thank Dr. Katsunori Sugimoto and Dr. Peter Burgers for providing RFC2/RFC4-Flag and rfc4 K55R/rfc4 K55E yeast strains, respectively, and Dr. Peter Burgers, Dr. Steven Brill, Dr. Ben Shen, and Dr. Steve West for providing plasmids used to purify the proteins and P. Kolesar for help with statistical analysis.
This work was supported by Research Support Programme (GAMU) - MUNI/M/1894/2014, Czech Science Foundation (GACR 13-26629S and 207/12/2323), project no. LQ1605 from the National Program of Sustainability II (MEYS CR).
Availability of data and materials
All data generated or analyzed in this study are included in this published article, its supplementary information files, and additional files.
LK designed the experiments. MS, VA, and AS purified the proteins. AS performed pull-down studies. VA constructed yeast strains and VA and AS performed genetic experiments and nuclease assays. LK and AS wrote the manuscript; All authors read and approved the final manuscript.
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The authors declare that they have no competing interests.
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