- Open Access
Cell size sensing—a one-dimensional solution for a three-dimensional problem?
© The Author(s). 2019
- Published: 29 April 2019
Individual cell types have characteristic sizes, suggesting that size sensing mechanisms may coordinate transcription, translation, and metabolism with cell growth rates. Two types of size-sensing mechanisms have been proposed: spatial sensing of the location or dimensions of a signal, subcellular structure or organelle; or titration-based sensing of the intracellular concentrations of key regulators. Here we propose that size sensing in animal cells combines both titration and spatial sensing elements in a dynamic mechanism whereby microtubule motor-dependent localization of RNA encoding importin β1 and mTOR, coupled with regulated local protein synthesis, enable cytoskeleton length sensing for cell growth regulation.
Early work in yeast and animal cells provided evidence for size sensing, with observations of non-linear growth rates and size-dependent fluctuations in growth duration between division points [8, 9]. However, these characteristics are not shared by all cell types studied to date; for example, analyses of proliferating rat Schwann cells suggested that they do not require a cell size checkpoint to maintain size . More recent studies on mammalian cell lines revealed a two-tier size homeostasis mechanism incorporating a size checkpoint with adder-like growth behavior . Mathematical modeling of size homeostasis behavior in single-cell datasets suggested that mammalian cells operate using a near-adder mode of size control, by combining modulation of both cell growth rate and cell-cycle progression . Indeed, another study using cell lines demonstrated longer growth times for smaller cells and adjustment of growth rates by larger cells before division . These findings, together with additional studies showing size dependence of transcription , protein synthesis [15, 16] or stabilization , and metabolism , suggest that size is likely sensed in eukaryotic cells while remaining enigmatic on the molecular details thereof. The likelihood of size-sensing mechanisms in animal cells is further highlighted by drastic phenotypes observed upon size disruption in mammalian neurons [19–21] and by reports proposing evolutionary links between metabolic activity and cell size [22, 23].
Despite accumulating evidence for size sensing capability in different cell types, the molecular details of such a mechanism are not well understood. Yeast cells have been most intensively studied in this regard, and two classes of size-sensing models have been proposed—titration-based measurements versus spatial sensing. Titration-based mechanisms postulate that increases or decreases in levels of a key signal provide a critical checkpoint size signal. A recent study in fission yeast demonstrated size-dependent expression of the mitotic activator Cdc25, and suggested that size-dependent increases in Cdc25 levels trigger cell division upon reaching a threshold concentration . An alternative mechanism based on work in budding yeast proposed that size-dependent reductions in concentration of the cell cycle inhibitor Whi5 is a key size regulator . Reconciliation of such apparent opposites might be achieved by combinatorial titration of multiple activator and inhibitor molecules whose levels are affected differentially by cell size . In this context, size might also be encoded by posttranslational or signaling modifications of the active molecules rather than absolute changes in their expression levels, as shown by a recent study linking p38 MAPK activity to size regulation in mammalian cell lines .
In the second class of models, subcellular localization of key signals provides size readouts to the cell. For example, in fission yeast the proteins Pom1 and Cdr2 have been proposed as components of such a mechanism, wherein Pom1 is transported to cell tips and diffuses from there to form longitudinal gradients along the cell, while Cdr2 is localized to large immobile structures at the plasma membrane in the cell middle, termed cortical nodes . Conflicting findings suggested that cell size was sensed either as length encoded by a linear Pom1 gradient [27, 28] or by cell surface area encoded by Cdr2p nodal concentration . More recent work has, however, suggested that cell size homeostasis is still preserved in Pom1 deletion mutants  and that Cdr nodal regulation is reinforced by additional components localizing in bursts to the nodes . Another very recent study suggests the existence of both Cdr2-dependent and Cdr2-independent size-sensing mechanisms in fission yeast . Thus, multiple levels of regulation and redundancy are likely to exist in size-sensing mechanisms, complicating elucidation of their key principles.
Other types of spatial measurements might also provide proxies for size sensing, such as monitoring the sizes of key organelles within a cell. Nuclear size is the most well-studied example, and the karyoplasmic ratio describes the tight nuclear/cytoplasm size relationship in almost any cycling cell type . Intriguingly, both nucleoplasm and cytoplasm harbor membrane-free structures and organelles that scale with cell size . Nucleolus size was shown to be linked to cell size by intracellular phase transitions driven by concentration changes upon successive cell divisions , and recent work in Caenorhabditis elegans intestine demonstrated a direct proportionality of nucleolus size to both cell and whole-body size throughout worm development . Centrosome size and microtubule cytoskeleton dimensions provide similar examples in the cytoplasm [37–39]; thus, for example, scaling of microtubule growth rates with cell size adapts mitotic spindle length to cell volume .
Correlations of overall microtubule cytoskeleton dimensions with cell size raises the possibility of using cytoskeleton length as a proxy measurement for size sensing, thus simplifying the three-dimensional challenge of size sensing to the single dimension of length measurement . Microtubules might be particularly appropriate for such measurements due to their spatial organization connecting the microtubule organizing center near the nucleus and cell center with the cortical region adjacent to the plasma membrane. Indeed, microtubule-associated transport has been implicated in length control of cilia or flagella, which are short linear projections extending a few microns from cell surfaces . A model based on retrograde diffusion of the microtubule motor kinesin after delivery of its cargo by anterograde transport suggested that it could act as a length sensor within flagella . A conceptually similar mechanism was previously proposed for length sensing during neuronal polarization, wherein anterograde transport and retrograde diffusion of an axon growth regulator accounts for its neurite length-dependent accumulation . Although such mechanisms might function well for organelles or small cells, the range limits of intracellular diffusion gradients likely restrict their applicability in large cells [41, 45].
We looked into the possibility that active transport by the microtubule motors dynein and kinesin coordinates length sensing, using neuronal axon length as a model system. Axons comprise the largest compartment of a neuron; hence, axon length provides a proxy for overall neuronal size. Moreover, the distributed morphologies and large sizes of neurons can be advantageous in studies of compartmentalized signaling and size sensing . Dynein and kinesin are inherently limited to unidirectional movement along microtubules, with characteristic velocities and transport capacity [47–49]. These characteristics provided useful constraints for modeling different configurations for motor-based length sensing [50, 51]. Simple models estimating length from signal spread or from duration of signal travel on a single motor type (the so-called “time of flight” model) were found to be unlikely by computational simulations due to noise effects and lack of robustness in the system . In contrast, simulations of a bidirectional motor model revealed length-correlated retrograde oscillating signals for configurations wherein a kinesin anterograde signal stimulates a dynein retrograde signal, which then in turn represses the anterograde signal . Oscillatory signals can be significantly more robust than amplitude-encoded signals [52, 53]; hence, encoding spatial information by signal frequency rather than signal quantity may be advantageous. The original simulations envisaged decoding of the oscillatory signal by biochemical or transcriptional networks in the cell , but a very recent modeling study suggested that this might also be done by spectral decomposition of the oscillatory signal . Calculations based on available measurements of velocities for molecular motors indicate that the model would be most appropriate for a range from tens of micrometers to a few millimeters , fitting the sizes of most animal cell types and embryonic neurons, but not small microorganisms or adult neurons in vivo in large mammals.
An experimental test of motor-dependent oscillatory signaling for axonal length sensing was suggested by simulations showing that reducing levels of either kinesin or dynein should slow frequency decay of the retrograde signal . If neuronal growth rates are proportional to retrograde signal frequency, or if growth stops when the system reaches a limiting frequency, the model predicts that reducing motor levels within a prescribed range should lead to increases in axon length . Indeed, a knockdown screen in sensory neurons revealed axon lengthening phenotypes upon reduced expression of dynein heavy chain 1 (Dync1h1) or a number of kinesin heavy chains. The heavy chains are the ATP-binding subunits of molecular motors and are indispensable to their function. Further analyses in a mouse line with a point mutation in Dync1h1 revealed increased axon lengths for both adult sensory neurons in culture and embryonic sensory axons in vivo . Moreover, cultured fibroblast cells from the mutant mouse also revealed size increases, suggesting that motor-based size sensing might also function in non-neuronal cells .
A follow-up study then examined a number of mouse mutants for axon-lengthening phenotypes similar to those observed upon microtubule motor knockdown, and identified such a phenotype in a mouse with an importin β1 3′ UTR deletion . Both adult sensory neurons in culture and embryonic sensory neurons in vivo revealed significantly more axon growth for the importin β1 3′ UTR deletion than wild-type controls . Since the main effect of this mutation is loss of importin β1 mRNA transport to axons, structure–function analyses were employed to identify the precise axon-localizing motif, which was then used to identify nucleolin as an RNA-binding protein (RBP) for importin β1 mRNA . Nucleolin is a multifunctional RBP that is a major component of the nucleolus, but is also found in the plasma membrane [58, 59]; hence, it is well-placed to function in a mechanism based on sensing distance between cell center and periphery.
Disruption of the interaction between nucleolin and kinesin using AS1411, a nucleolin-targeted DNA aptamer, sequestered nucleolin from sensory axons and caused robust increases in axon growth . Similar findings were obtained in 3 T3 fibroblast cells, where we found importin β1 mRNA associated with kinesin and nucleolin, and importin β1 protein associated with dynein. Strikingly, AS1411 treatment of 3 T3 cells caused a significant size increase at all stages of the cell cycle . Moreover, aptamer treatment also induced a significant reduction in local protein synthesis at axon tips of cultured neurons and in the cortical domains of fibroblast cells . A similar reduction in protein synthesis at axon tips was also observed in cultures of importin β1 3′ UTR deletion neurons . Hence, perturbation of the subcellular localization of nucleolin or of its cargo importin β1 mRNA affects axon length or cell size. In this context, it is interesting to note that subcellular partitioning of importin α to the plasma membrane was very recently suggested to scale nucleus and mitotic spindle size to cell size . It will be interesting to explore the relationship between membrane association and motor-driven cytoplasmic transport of importins in size regulation mechanisms.
The text above, summarized in Fig. 2, outlines a hypothesis for cell size sensing that still requires extensive testing on multiple levels. Attractive features of this hypothesis include simplifying the three-dimensional challenge of cell size sensing to scanning the single dimension of cytoskeletal length, increased robustness due to frequency encoding rather than amplitude encoding of size signals, and the combination of features of both spatial and titration-based modes of size sensing. Indeed, one might envisage an evolutionary continuum in the development of such mechanisms. Purely titration-based sensing of key protein concentrations might have provided size readouts in early and small morphologically simple cells where diffusion ensured uniformity of the readout throughout the cell. As cells evolved to become larger and morphologically complex, protein levels could become differentially regulated in subcellular compartments, driving addition of spatial specifications to the initial titration-based size sensing mechanism. Different cell types may have evolved to utilize various combinations of these principles to fit their specific morphological constraints in size sensing. Motor-driven RNA-based localization of protein synthesis regulators allows differential regulation of biosynthesis in different cellular compartments, potentially combining titration and spatial sensing elements into one combined mechanism for large morphologically complex cells. Further characterization of the mechanism will require identification of feedback components in the system and determining how localized changes in protein synthesis can provide size readouts to cells.
We most sincerely thank the BMC Biology editors and five anonymous reviewers for their help in improving the manuscript, and Dr. Dalia Gordon for comments on the initial draft.
Our work in this field was supported by the European Research Council (Neurogrowth) and the Israel Science Foundation (1337/18). M.F. is the incumbent of the Chaya Professorial Chair in Molecular Neuroscience at the Weizmann Institute of Science.
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I.R. and M.F. wrote the manuscript and both authors have read and agreed to the final content.
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- Marshall WF, Young KD, Swaffer M, Wood E, Nurse P, Kimura A, Frankel J, Wallingford J, Walbot V, Qu X, et al. What determines cell size? BMC Biol. 2012;10:101.PubMedPubMed CentralView ArticleGoogle Scholar
- Westfall CS, Levin PA. Bacterial cell size: multifactorial and multifaceted. Annu Rev Microbiol. 2017;71(1):499–517.PubMedPubMed CentralView ArticleGoogle Scholar
- Willis L, Huang KC. Sizing up the bacterial cell cycle. Nat Rev Microbiol. 2017;15(10):606–20.PubMedView ArticleGoogle Scholar
- Wallden M, Fange D, Lundius EG, Baltekin O, Elf J. The synchronization of replication and division cycles in individual E. coli cells. Cell. 2016;166(3):729–39.PubMedView ArticleGoogle Scholar
- Banerjee S, Lo K, Daddysman MK, Selewa A, Kuntz T, Dinner AR, Scherer NF. Biphasic growth dynamics control cell division in Caulobacter crescentus. Nat Microbiol. 2017;2:17116.PubMedView ArticleGoogle Scholar
- Chandler-Brown D, Schmoller KM, Winetraub Y, Skotheim JM. The adder phenomenon emerges from independent control of pre- and post-start phases of the budding yeast cell cycle. Curr Biol. 2017;27(18):2774–83 e2773.PubMedPubMed CentralView ArticleGoogle Scholar
- Eun YJ, Ho PY, Kim M, LaRussa S, Robert L, Renner LD, Schmid A, Garner E, Amir A. Archaeal cells share common size control with bacteria despite noisier growth and division. Nat Microbiol. 2018;3(2):148–54.PubMedView ArticleGoogle Scholar
- Wood E, Nurse P. Sizing up to divide: mitotic cell-size control in fission yeast. Annu Rev Cell Dev Biol. 2015;31:11–29.PubMedView ArticleGoogle Scholar
- Ginzberg MB, Kafri R, Kirschner M. Cell biology. On being the right (cell) size. Science. 2015;348(6236):1245075.PubMedPubMed CentralView ArticleGoogle Scholar
- Conlon I, Raff M. Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression. J Biol. 2003;2(1):7.PubMedPubMed CentralView ArticleGoogle Scholar
- Varsano G, Wang Y, Wu M. Probing mammalian cell size homeostasis by channel-assisted cell reshaping. Cell Rep. 2017;20(2):397–410.PubMedView ArticleGoogle Scholar
- Cadart C, Monnier S, Grilli J, Saez PJ, Srivastava N, Attia R, Terriac E, Baum B, Cosentino-Lagomarsino M, Piel M. Size control in mammalian cells involves modulation of both growth rate and cell cycle duration. Nat Commun. 2018;9(1):3275.PubMedPubMed CentralView ArticleGoogle Scholar
- Ginzberg MB, Chang N, D'Souza H, Patel N, Kafri R, Kirschner MW. Cell size sensing in animal cells coordinates anabolic growth rates and cell cycle progression to maintain cell size uniformity. eLife. 2018;7:e26957.Google Scholar
- Marguerat S, Bähler J. Coordinating genome expression with cell size. Trends Genet. 2012;28(11):560–5.PubMedView ArticleGoogle Scholar
- Goranov AI, Gulati A, Dephoure N, Takahara T, Maeda T, Gygi SP, Manalis S, Amon A. Changes in cell morphology are coordinated with cell growth through the TORC1 pathway. Curr Biol. 2013;23(14):1269–79.PubMedView ArticleGoogle Scholar
- Schmoller KM, Turner JJ, Koivomagi M, Skotheim JM. Dilution of the cell cycle inhibitor Whi5 controls budding-yeast cell size. Nature. 2015;526(7572):268–72.PubMedPubMed CentralView ArticleGoogle Scholar
- Acebron SP, Karaulanov E, Berger BS, Huang YL, Niehrs C. Mitotic wnt signaling promotes protein stabilization and regulates cell size. Mol Cell. 2014;54(4):663–74.PubMedView ArticleGoogle Scholar
- Miettinen TP, Pessa HK, Caldez MJ, Fuhrer T, Diril MK, Sauer U, Kaldis P, Bjorklund M. Identification of transcriptional and metabolic programs related to mammalian cell size. Curr Biol. 2014;24(6):598–608.PubMedPubMed CentralView ArticleGoogle Scholar
- Rooney GE, Goodwin AF, Depeille P, Sharir A, Schofield CM, Yeh E, Roose JP, Klein OD, Rauen KA, Weiss LA, et al. Human iPS cell-derived neurons uncover the impact of increased Ras signaling in Costello syndrome. J Neurosci. 2016;36(1):142–52.PubMedPubMed CentralView ArticleGoogle Scholar
- Deshpande A, Yadav S, Dao DQ, Wu ZY, Hokanson KC, Cahill MK, Wiita AP, Jan YN, Ullian EM, Weiss LA. Cellular phenotypes in human iPSC-derived neurons from a genetic model of autism spectrum disorder. Cell Rep. 2017;21(10):2678–87.PubMedPubMed CentralView ArticleGoogle Scholar
- Roy A, Skibo J, Kalume F, Ni J, Rankin S, Lu Y, Dobyns WB, Mills GB, Zhao JJ, Baker SJ, et al. Mouse models of human PIK3CA-related brain overgrowth have acutely treatable epilepsy. eLife. 2015;4:e12703.Google Scholar
- Miettinen TP, Caldez MJ, Kaldis P, Bjorklund M. Cell size control - a mechanism for maintaining fitness and function. Bioessays. 2017;39(9). https://doi.org/10.1002/bies.201700058.View ArticleGoogle Scholar
- Anzi S, Stolovich-Rain M, Klochendler A, Fridlich O, Helman A, Paz-Sonnenfeld A, Avni-Magen N, Kaufman E, Ginzberg MB, Snider D, et al. Postnatal exocrine pancreas growth by cellular hypertrophy correlates with a shorter lifespan in mammals. Dev Cell. 2018;45(6):726–37 e723.PubMedView ArticleGoogle Scholar
- Keifenheim D, Sun XM, D'Souza E, Ohira MJ, Magner M, Mayhew MB, Marguerat S, Rhind N. Size-dependent expression of the mitotic activator Cdc25 suggests a mechanism of size control in fission yeast. Curr Biol. 2017;27(10):1491–7 e1494.PubMedPubMed CentralView ArticleGoogle Scholar
- Schmoller KM, Skotheim JM. The biosynthetic basis of cell size control. Trends Cell Biol. 2015;25(12):793–802.PubMedView ArticleGoogle Scholar
- Liu S, Ginzberg MB, Patel N, Hild M, Leung B, Li Z, Chen YC, Chang N, Wang Y, Tan C, et al. Size uniformity of animal cells is actively maintained by a p38 MAPK-dependent regulation of G1-length. eLife. 2018;7:e26947.Google Scholar
- Martin SG, Berthelot-Grosjean M. Polar gradients of the DYRK-family kinase Pom1 couple cell length with the cell cycle. Nature. 2009;459(7248):852–6.PubMedView ArticleGoogle Scholar
- Moseley JB, Mayeux A, Paoletti A, Nurse P. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature. 2009;459(7248):857–60.PubMedView ArticleGoogle Scholar
- Pan KZ, Saunders TE, Flor-Parra I, Howard M, Chang F. Cortical regulation of cell size by a sizer cdr2p. eLife. 2014;3:e02040.PubMedPubMed CentralView ArticleGoogle Scholar
- Wood E, Nurse P. Pom1 and cell size homeostasis in fission yeast. Cell Cycle. 2013;12(19):3228–36.PubMedPubMed CentralView ArticleGoogle Scholar
- Allard CAH, Opalko HE, Liu KW, Medoh U, Moseley JB. Cell size-dependent regulation of Wee1 localization by Cdr2 cortical nodes. J Cell Biol. 2018;217(5):1589–99.PubMedPubMed CentralView ArticleGoogle Scholar
- Facchetti G, Knapp B, Flor-Parra I, Chang F, Howard M. Reprogramming Cdr2-dependent geometry-based cell size control in fission yeast. Curr Biol. 2019;29(2):350–8 e354.PubMedPubMed CentralView ArticleGoogle Scholar
- Mukherjee RN, Chen P, Levy DL. Recent advances in understanding nuclear size and shape. Nucleus. 2016;7(2):167–86.PubMedPubMed CentralView ArticleGoogle Scholar
- Brangwynne CP. Phase transitions and size scaling of membrane-less organelles. J Cell Biol. 2013;203(6):875–81.PubMedPubMed CentralView ArticleGoogle Scholar
- Weber SC, Brangwynne CP. Inverse size scaling of the nucleolus by a concentration-dependent phase transition. Curr Biol. 2015;25(5):641–6.PubMedPubMed CentralView ArticleGoogle Scholar
- Uppaluri S, Weber SC, Brangwynne CP. Hierarchical size scaling during multicellular growth and development. Cell Rep. 2016;17(2):345–52.PubMedView ArticleGoogle Scholar
- Decker M, Jaensch S, Pozniakovsky A, Zinke A, O'Connell KF, Zachariae W, Myers E, Hyman AA. Limiting amounts of centrosome material set centrosome size in C. elegans embryos. Curr Biol. 2011;21(15):1259–67.PubMedView ArticleGoogle Scholar
- Mitchison TJ, Ishihara K, Nguyen P, Wuhr M. Size scaling of microtubule assemblies in early Xenopus embryos. Cold Spring Harb Perspect Biol. 2015;7(10):a019182.PubMedPubMed CentralView ArticleGoogle Scholar
- Spencer AK, Schaumberg AJ, Zallen JA. Scaling of cytoskeletal organization with cell size in drosophila. Mol Biol Cell. 2017;28(11):1519–29.PubMedPubMed CentralView ArticleGoogle Scholar
- Lacroix B, Letort G, Pitayu L, Salle J, Stefanutti M, Maton G, Ladouceur AM, Canman JC, Maddox PS, Maddox AS, et al. Microtubule dynamics scale with cell size to set spindle length and assembly timing. Dev Cell. 2018;45(4):496–511 e496.PubMedView ArticleGoogle Scholar
- Albus CA, Rishal I, Fainzilber M. Cell length sensing for neuronal growth control. Trends Cell Biol. 2013;23(7):305–10.PubMedView ArticleGoogle Scholar
- Lechtreck KF, Van De Weghe JC, Harris JA, Liu P. Protein transport in growing and steady-state cilia. Traffic. 2017;18(5):277–86.PubMedPubMed CentralView ArticleGoogle Scholar
- Hendel NL, Thomson M, Marshall WF. Diffusion as a ruler: modeling kinesin diffusion as a length sensor for intraflagellar transport. Biophys J. 2018;114(3):663–74.PubMedPubMed CentralView ArticleGoogle Scholar
- Toriyama M, Sakumura Y, Shimada T, Ishii S, Inagaki N. A diffusion-based neurite length-sensing mechanism involved in neuronal symmetry breaking. Mol Syst Biol. 2010;6:394.PubMedPubMed CentralView ArticleGoogle Scholar
- Munoz-Garcia J, Kholodenko BN. Signalling over a distance: gradient patterns and phosphorylation waves within single cells. Biochem Soc Trans. 2010;38(5):1235–41.PubMedView ArticleGoogle Scholar
- Terenzio M, Schiavo G, Fainzilber M. Compartmentalized signaling in neurons: from cell biology to neuroscience. Neuron. 2017;96(3):667–79.PubMedView ArticleGoogle Scholar
- Schiavo G, Greensmith L, Hafezparast M, Fisher EMC. Cytoplasmic dynein heavy chain: the servant of many masters. Trends Neurosci. 2013;36(11):641–51.PubMedPubMed CentralView ArticleGoogle Scholar
- Reck-Peterson SL, Redwine WB, Vale RD, Carter AP. The cytoplasmic dynein transport machinery and its many cargoes. Nat Rev Mol Cell Biol. 2018;19(6):382–98.PubMedPubMed CentralView ArticleGoogle Scholar
- Hirokawa N, Tanaka Y. Kinesin superfamily proteins (KIFs): various functions and their relevance for important phenomena in life and diseases. Exp Cell Res. 2015;334(1):16–25.PubMedView ArticleGoogle Scholar
- Kam N, Pilpel Y, Fainzilber M. Can molecular motors drive distance measurements in injured neurons? PLoS Comput Biol. 2009;5(8):e1000477.PubMedPubMed CentralView ArticleGoogle Scholar
- Rishal I, Kam N, Perry RB, Shinder V, Fisher EM, Schiavo G, Fainzilber M. A motor-driven mechanism for cell-length sensing. Cell Rep. 2012;1(6):608–16.PubMedPubMed CentralView ArticleGoogle Scholar
- Tostevin F, de Ronde W, ten Wolde PR. Reliability of frequency and amplitude decoding in gene regulation. Phys Rev Lett. 2012;108(10):108104.PubMedView ArticleGoogle Scholar
- de Ronde W, ten Wolde PR. Multiplexing oscillatory biochemical signals. Phys Biol. 2014;11(2):026004.PubMedView ArticleGoogle Scholar
- Smedler E, Uhlen P. Frequency decoding of calcium oscillations. Biochim Biophys Acta. 2014;1840(3):964–9.PubMedView ArticleGoogle Scholar
- Folz F, Wettmann L, Morigi G, Kruse K. Can you hear an axon growing? arXiv. 2018;1807:04799.Google Scholar
- Perry Rotem B-T, Doron-Mandel E, Iavnilovitch E, Rishal I, Dagan Shachar Y, Tsoory M, Coppola G, McDonald Marguerite K, Gomes C, Geschwind Daniel H, et al. Subcellular knockout of importin β1 perturbs axonal retrograde signaling. Neuron. 2012;75(2):294–305.PubMedPubMed CentralView ArticleGoogle Scholar
- Perry RB, Rishal I, Doron-Mandel E, Kalinski AL, Medzihradszky KF, Terenzio M, Alber S, Koley S, Lin A, Rozenbaum M, et al. Nucleolin-mediated RNA localization regulates neuron growth and cycling cell size. Cell Rep. 2016;16(6):1664–76.PubMedPubMed CentralView ArticleGoogle Scholar
- Berger CM, Gaume X, Bouvet P. The roles of nucleolin subcellular localization in cancer. Biochimie. 2015;113:78–85.PubMedView ArticleGoogle Scholar
- Ugrinova I, Petrova M, Chalabi-Dchar M, Bouvet P. Multifaceted nucleolin protein and its molecular partners in oncogenesis. Adv Protein Chem Struct Biol. 2018;111:133–64.PubMedView ArticleGoogle Scholar
- Brownlee C, Heald R. Importin alpha partitioning to the plasma membrane regulates intracellular scaling. Cell. 2019;176(4):805–15 e808.PubMedView ArticleGoogle Scholar
- Fonseca BD, Smith EM, Yelle N, Alain T, Bushell M, Pause A. The ever-evolving role of mTOR in translation. Semin Cell Dev Biol. 2014;36:102–12.PubMedView ArticleGoogle Scholar
- Iadevaia V, Liu R, Proud CG. mTORC1 signaling controls multiple steps in ribosome biogenesis. Semin Cell Dev Biol. 2014;36:113–20.PubMedView ArticleGoogle Scholar
- Delarue M, Brittingham GP, Pfeffer S, Surovtsev IV, Pinglay S, Kennedy KJ, Schaffer M, Gutierrez JI, Sang D, Poterewicz G, et al. mTORC1 controls phase separation and the biophysical properties of the cytoplasm by tuning crowding. Cell. 2018;174(2):338–49 e320.PubMedView ArticleGoogle Scholar
- Saxton RA, Sabatini DM. mTOR signaling in growth, metabolism, and disease. Cell. 2017;168(6):960–76.PubMedPubMed CentralView ArticleGoogle Scholar
- Lucena R, Alcaide-Gavilan M, Schubert K, He M, Domnauer MG, Marquer C, Klose C, Surma MA, Kellogg DR. Cell size and growth rate are modulated by TORC2-dependent signals. Curr Biol. 2018;28(2):196–210 e194.PubMedView ArticleGoogle Scholar
- Betz C, Hall MN. Where is mTOR and what is it doing there? J Cell Biol. 2013;203(4):563–74.PubMedPubMed CentralView ArticleGoogle Scholar
- Ebner M, Sinkovics B, Szczygiel M, Ribeiro DW, Yudushkin I. Localization of mTORC2 activity inside cells. J Cell Biol. 2017;216(2):343–53.PubMedPubMed CentralView ArticleGoogle Scholar
- Terenzio M, Koley S, Samra N, Rishal I, Zhao Q, Sahoo PK, Urisman A, Marvaldi L, Oses-Prieto JA, Forester C, et al. Locally translated mTOR controls axonal local translation in nerve injury. Science. 2018;359(6382):1416–21.PubMedView ArticleGoogle Scholar
- Clippinger AJ, Alwine JC. Dynein mediates the localization and activation of mTOR in normal and human cytomegalovirus-infected cells. Genes Dev. 2012;26(18):2015–26.PubMedPubMed CentralView ArticleGoogle Scholar