- Research article
- Open Access
Comparative analysis of Klebsiella pneumoniae genomes identifies a phospholipase D family protein as a novel virulence factor
© Lery et al.; licensee BioMed Central Ltd. 2014
- Received: 14 May 2014
- Accepted: 15 May 2014
- Published: 29 May 2014
Klebsiella pneumoniae strains are pathogenic to animals and humans, in which they are both a frequent cause of nosocomial infections and a re-emerging cause of severe community-acquired infections. K. pneumoniae isolates of the capsular serotype K2 are among the most virulent. In order to identify novel putative virulence factors that may account for the severity of K2 infections, the genome sequence of the K2 reference strain Kp52.145 was determined and compared to two K1 and K2 strains of low virulence and to the reference strains MGH 78578 and NTUH-K2044.
In addition to diverse functions related to host colonization and virulence encoded in genomic regions common to the four strains, four genomic islands specific for Kp52.145 were identified. These regions encoded genes for the synthesis of colibactin toxin, a putative cytotoxin outer membrane protein, secretion systems, nucleases and eukaryotic-like proteins. In addition, an insertion within a type VI secretion system locus included sel1 domain containing proteins and a phospholipase D family protein (PLD1). The pld1 mutant was avirulent in a pneumonia model in mouse. The pld1 mRNA was expressed in vivo and the pld1 gene was associated with K. pneumoniae isolates from severe infections. Analysis of lipid composition of a defective E. coli strain complemented with pld1 suggests an involvement of PLD1 in cardiolipin metabolism.
Determination of the complete genome of the K2 reference strain identified several genomic islands comprising putative elements of pathogenicity. The role of PLD1 in pathogenesis was demonstrated for the first time and suggests that lipid metabolism is a novel virulence mechanism of K. pneumoniae.
- Genome sequencing
- Host-microbe interactions
- Bacterial pathogenesis
- Lipid metabolism
Klebsiella pneumoniae is responsible for a variety of diseases in humans and animals. As a prominent nosocomial pathogen it is mainly responsible for urinary tract, respiratory tract or blood infections [1–3]. In addition, because of the acquisition of extended-spectrum β-lactamases and carbapenemases, such as the recently described NDM-1 , multi, extremely or pan-drug resistant clinical strains are more frequently isolated [5, 6]. In addition, K. pneumoniae has re-emerged as a cause of community-acquired infections including pneumonia and the characteristic syndrome of pyogenic liver abscess, with possible complications including endophthalmitis or meningitis [7, 8]. K. pneumoniae is, thus, an important virulent pathogen able to cause serious infections in ambulatory, otherwise healthy hosts and to spread within patients [5, 9] that requires a better understanding of the molecular mechanisms underlying the various forms of its pathogenesis.
Major K. pneumoniae virulence factors include the capsule, the lipopolysaccharide, iron scavenging systems and adhesins [3, 10–14]. The capsule is one of the most important virulence determinants, protecting against serum bactericidal activity, antimicrobial peptides and phagocytosis [11, 15–18]. At least 77 capsular (K) types can be distinguished, but types K1 and K2 are prominent by their virulence in murine models of infection [19–21] and by their epidemiological prevalence [9, 18, 21, 22]. However, not all K1/K2-type strains are necessarily virulent, as distinct clonal groups of K1 and K2 differ sharply by their virulence . Reference strain Kp52.145 (derived from B5055, the reference strain of serotype K2) is a highly virulent strain from which important virulence factors, including the large virulence plasmid harboring the regulator of mucoid phenotype (rmpA) and the aerobactin cluster, were identified [10, 11, 21]. Even though a virulence plasmid-cured strain is less virulent than the parental strain, it remained more virulent than other isolates that do not harbor this plasmid , showing that factors other than capsule overexpression and aerobactin account for the higher pathogenesis of this strain. Therefore, although known virulence factors are certainly crucial for bacterial survival, protection and interaction with the host, putative new virulence factors that could subvert host cell physiology and response remain yet to be identified.
Comparative genomics of pathogenic and non-pathogenic strains is a powerful approach to identify putative virulence genes. Several draft or complete genomes of clinical isolates of K. pneumoniae have been published so far, but only the virulent serotype K1 strain NTUH-K2044  has been described in detail. In order to identify new K. pneumoniae K2 virulence factors, we sequenced the genome of the virulent strain Kp52.145, as well as two additional strains of low virulence, SB2390 (serotype K2) and SB3193 (serotype K1). By comparing these three novel genomes to the publicly available genomes of the virulent K1 strain NTUH-K2044 and reference strain MGH 78578, we identified in Kp52.145 putative virulence genes and analyzed their distribution within a diverse collection of K. pneumoniae strains. We demonstrate that a gene coding for a phospholipase D family protein (PLD1), located within a type VI secretion system locus, is expressed in vivo, is involved in controlling bacterial membrane lipid composition, and is a new virulence factor.
Genome assembly and annotation
General features of the K. pneumoniae genomes analyzed
Strain bank ID
Wu et al. 
Because Kp52.145 is a highly virulent strain, our analyses were focused on comparing its genome with the genomes of K. pneumoniae strains SB2390, SB3193, NTUH-K2044 and MGH78578. According to SEED subsystems annotations , about 60% of protein-coding genes for each K. pneumoniae genome had predicted functions. More specifically, the largest percentage of annotated genes is involved in the metabolism of carbohydrates (approximately 20%), of amino acids and their derivatives (approximately 10%) and of cofactors, vitamins and prosthetic groups (approximately 8%) [see Additional file 1].
To define the common genome of the five strains, we used stringent BlastClust parameters of at least 90% identity and at least 80% coverage. This analysis identified 3,587 coding sequences common to the five genomes. The majority of proteins are involved in metabolic processes, such as energy metabolism and transporters, supporting the general concept that the core genome encompasses essential functions required for survival of the microorganism. The K. pneumoniae core genome comprised several sets of genes whose functions are related to bacterial survival in the environment or interaction with its host, and possibly virulence. This was the case, for example, of genes involved in quorum-sensing and biofilm formation, adhesins, and secretion systems, for which examples are detailed below.
Genes encoding for autoinducer-2 and type III fimbria, involved in biofilm formation in K. pneumoniae[26–28], were present in all sequenced strains. In addition, genes coding for synthesis and transport of the poly-β-1,6-N-acetyl-D-glucosamine (PGA) adhesin (KpST66_4915 to KpST66_4918 in Kp52.145 genome) which is required for the structural stability of Escherichia coli biofilms , and YidE (KpST66_0019), which mediates the hyperadherence phenotype of E. coli, were also found in the core genome of K. pneumoniae strains.
Moreover, the five K. pneumoniae genomes contained the genes barA/uvrY (respectively KPST66_0986 and KpST66_3517) and ycjX/ycjF (KpST66_2441 and KpST66_2442) that may be involved in bacterial fitness and virulence. The two-component system BarA/UvrY (KpST66_3517 and KpST66_0986 in Kp52.145 genome) contributes to biofilm formation in Salmonella enterica and is a virulence determinant of urinary tract E. coli infections [31, 32]. The E. coli YcjF protein is expressed in a septicemia murine model of infection in which the ycjF mutant is attenuated, thus suggesting its implication in the in vivo survival/multiplication of the bacteria .
Distribution of virulence-related factors among K. pneumoniae genomes analyzed
Regulator of capsule expression
Conjugative machinery/protein secretion
Binding to extra cellular matrix compounds
Potentially hijack IL17R signaling pathways
Binding to hydrophobic ligands / putative regulation of homeostasis and immunity
Furthermore, the core genome presented a large genomic region located between gly-tRNA and phe-tRNA loci in Kp52.145 genome, containing frdABCD genes coding for the fumarate reductase enzymatic complex responsible for fumarate respiration under anaerobic growth of bacteria. This complex is a virulence determinant for Helicobacter pylori, Mycobacterium tuberculosis, Actinobacillus pleuropneumoniae and S. enterica, as mutants on these genes are attenuated [40–43]. The ability to grow anaerobically allows bacterial pathogens to persist in host tissues, including in the lungs. Curiously, this genomic locus encoded in K. pneumoniae at least one more protein involved in anaerobic metabolism, the anaerobic C4-dicarboxylate transporter DcuA (KpST66_4904), supporting the idea that this GI provides K. pneumoniae advantages to grow under anaerobic conditions, possibly favoring infection. Additional proteins that might be involved in bacterial fitness to environmental stress conditions were encoded in this region. For instance, the putative small multidrug resistance protein SugE (KpST66_4882) has been shown to regulate biofilm formation and capsule expression . A lipocalin-2 bacterial protein Bcl (KpST66_4881) is also encoded in this island of the genome. Eukaryotic lipocalins are small extracellular proteins that bind hydrophobic ligands and fulfill numerous biological functions including regulation of cellular homeostasis and immunity and are regulators of antibacterial defense . Lipocalin2 is for instance a siderophore scavenger for several bacteria, including K. pneumoniae, as well as a negative regulator of inflammatory response during Streptococcus pneumoniae pneumonia . However, the role of bacterial lipocalins is not yet known.
Finally, a phosphatidylserine decarboxylase (KpST66_4873) might be important for the integrity of the bacterial membrane composition, as phospholipid biosynthetic pathways play crucial roles in the virulence of several pathogens [48, 49].
The accessory genome (genes absent in at least one of the five strains) included a large number of specific coding sequences (CDSs): 743 genes were found only in Kp52.145, 608 in NTUH-K2044, 806 in SB3193, 635 in SB2390 and 488 in MGH78578. About 50% of these genes code for hypothetical proteins or proteins of unknown function. The distribution of the putative virulence-related genes of K. pneumoniae among the sequenced strains is summarized in Table 2. The specific regions or genes of Kp52.145 are detailed below.
Strain Kp52.145 possessed two plasmids (Figure 1B). The first plasmid of 121 Kb carried the aerobactin cluster and the regulator of mucoid phenotype rmpA genes. The presence of this plasmid was previously correlated with the virulence of K. pneumoniae K1 and K2 isolates [21, 50]. Additionally, a strain cured from this plasmid showed a 6 × 104-fold reduction in virulence, establishing the link between this plasmid and bacterial virulence . In addition to aerobactin and rmpA, this plasmid contained genes coding for F-pilin, purine metabolism, insertion sequences and proteins of unknown functions. Kp52.145 also carried a second, previously non-described, plasmid of about 90 Kb. Interestingly, it contained rmpA2, a homologue of the regulator of mucoid phenotype rmpA, which seems to be involved in capsule expression regulation [51, 52]. F-pilin genes, a subtilisin-related serine protease, an AAA + ATPase, the UV protection system UmuD/UmuC and the genes encoding the toxin-antitoxin systems RelE/orf4 and VagD/VagC (Figure 1B) were also found on this plasmid. However, the potential role of these genes in virulence remains to be investigated.
Genomic islands identified in the genome of strain Kp52.145
Genomic island 1 (GI-1): ICE-Kp1-like region
The largest GI found in the Kp52.145 genome comprised 133,679 bp, presented a GC content of 52%, was inserted in an asn-tRNA locus and encoded 92 CDSs (Figure 2A). Most of the protein-coding genes found in this region were described as part of the IceKpI GI of NTUH-K2044 [55, 56], although several differences were found. Kp52.145 GI-I begins at asn tRNA locus followed by several uncharacterized proteins and insertion sequence elements. GI-I coded for the synthesis of two polyketide/nonribosomal peptides (yersiniabactin and colibactin) and for the conjugative transfer machinery (T4SS) that allows horizontal transfer of the island . In contrast to the previous description of IceKpI , the Kp52.145_GI-I carried colibactin and did not contain the region coding for vagC-vagD, iroN-iroB-iroC-iroD and rmpA genes which are carried only by the 121 kB plasmid in Kp52.145.
Genomic island 2 (GI-II)
We describe here a novel GI (GI-II). It is a 29,829 bp island, with a GC content of 49% and coding for 28 CDSs which is inserted in a leu-tRNA locus (Figure 2B). Potential pathogenesis-related genes coded for a putative cytotoxic outer membrane protein (cOMP, KpST66_4736) and a polyamine ABC transport system (KpST66_4729 to KpST66_4732). cOMP closest known homologue (34% identity) was a Plesiomonas shigelloides predominant virulence factor proposed to trigger cell death in host cells following infection . Polyamine biosynthesis and transport mechanisms were intricately linked to fitness, survival, biofilm formation and pathogenesis, for instance in S. pneumoniae and Yersinia pestis[58, 59]. Additionally, this GI encoded a 4-phytase gene (KpST66_4736), ugpQ3 (KpST66_4728) and xylA, xynT, xynB, xylR (KpST66_4724 to KpST66_4727) that are involved in xylose metabolism.
Genomic island 3 (GI-III)
The third GI is characterized by a 49,657 bp region presenting a G + C content of 51% and contained 66 CDSs, most of them coding for phage structural proteins (Figure 2C). This GI included genes coding for proteins with homologues that were shown to play a role in bacterial adhesion and immune system escape [60–62]. These proteins encoded for an immunoglobulin domain-containing protein (KpST66_1506), a peptidase S24-like protein (KpST66_1511), two HNH family endonucleases (KpST66_1468 and 1486) and an exonuclease (locus 1464).
Genomic island 4 (GI-IV)
The forth island (GI-IV) was mainly comprised of phage-related genes. Among the 42 CDSs encoded within this genomic region, one gene coded for a SEFIR-domain containing protein (KpST66_1945; Figure 2D). A SEFIR domain is usually found in IL17 receptors and SEF proteins, acting in eukaryotes signaling pathways. Very little is known about prokaryotic SEFIR-containing proteins. Structural analyses suggested that these bacterial SEFIR domains share structural and electrostatic similarity with their mammalian homologues and, thereby, could potentially subvert host immunity by hijacking the IL17R signaling pathways . Notably, local production of IL-17 is a significant factor in effective host defense against Gram-negative bacteria, including K. pneumoniae. Therefore, further studies are required to elucidate whether KpST66_1945 is implicated in K. pneumoniae pathogenesis.
Distribution of GIs among K. pneumoniaestrains
Prevalence of the genomic islands among K. pneumoniae isolates
T6SS locus III insertion
Recently, three different T6SS loci were defined in K. pneumoniae. Within these loci, three putative valine-glycine repeat (Vgr) proteins and two hemolysin-coregulated proteins (Hcp) were described as potential effector proteins, through their sequence similarities to Vibrio cholerae and Pseudomonas aeruginosa effector proteins [65–68]. Accordingly, the Kp52.145 genome also presented three conserved T6SS loci syntenic to those previously described. The first two loci were identical in composition and orientation to the previously described ones. The third one, locus III, had conservation of adjacency limited to the imcF/impG/impH and impJ/ompA/vgrG gene clusters, as a region with nine genes was inserted between these two clusters. This insertion encoded for one hypothetical protein, five putative Sel-1 repeat containing lipoproteins and three putative phospholipase D family proteins (Figure 1A). Flanking sequences suggesting how this region was inserted were not found.
In order to obtain evidence that the product of these three genes coding either for full length or partial PLD-family proteins are important for bacterial survival in vivo, we checked by RT-PCR for their mRNA expression. We observed that these genes are expressed both in bacteria grown for four hours in Trypto Casein Soy broth (GTCS) medium, as well as in the lungs of mice infected for 24 hours (data not shown). These results prompted us to further check for a putative involvement of the full length PLD gene, called pld1, on K. pneumoniae virulence.
Involvement of the phospholipase D family protein gene pld1in KP 52.145 virulence
To analyze the frequency and clonal distribution of the pld1 gene in K. pneumoniae, the 171 genomes were analyzed using BlastN. We observed that besides ST66, represented by strain Kp52.145, pld1 was present in 10 strains (6.4%) belonging to ST380, ST679, ST67 (K. pneumoniae subsp. rhinoscleromatis) and ST35, but in none of the other isolates. It is interesting to note that ST380 was associated with severe K. pneumoniae infections [9, 65] and that K. pneumoniae subsp. rhinoscleromatis is the only Klebsiella strain to be able to survive intracellularly in macrophages .
Functional characterization of PLD1
As mentioned above, the bacterial PLD-family proteins can be classified in four subfamilies. One of them, the cardiolipin synthase is able to convert two PG molecules into glycerol and cardiolipin, or to catalyze the opposite reaction, leading to PG formation . Our results suggest that PLD1 belongs to the cardiolipin synthase subfamily and that it plays a role in balancing the PG and cardiolipin content.
It has been shown that humans and mice with bacterial pneumonia have markedly elevated amounts of cardiolipin in lung fluid and that it impairs surfactant function, lung mechanics, modulation of cell survival and cytokine networks and lung consolidation .
There is evidence that bacteria are able to adjust their relative concentrations of phosphatidylethanolamine and PGs when subjected to environmental stresses. Such an alteration in headgroup composition seems to be a means for changing membrane permeability and, hence, preserving stability . Therefore, we hypothesize that PLD1 alters the membrane composition and charge, affecting bacterial interaction with the host environment.
Recently, Russel el. al. demonstrated that diverse phospholipase proteins encoded within the T6SS loci of several prokaryotic genomes are antibacterial effectors, conferring competitive advantages on the donor strain during interbacterial interactions . These proteins are generally designated as ‘T6SS Lipase Effectors’ (Tle) and classified in five sub-families, according to the sequence conservation and number of catalytic motifs present. Kp52.145 PLD-family protein could be considered a Tle5 member, as it presents two conserved HxKxxxxD motifs. However, in the Kp52.145 genome we did not identify any gene similar to the cognate immunity genes - a hallmark of the genomic islands described by Russel et al. Moreover, we did not observe such an antibacterial effect of Kp52.145 or its PLD1 mutant strain upon competition with E. coli [see Additional file 1]. These results showed that pld1 is implicated in virulence without being an anti-bacterial factor and is, so far, unique.
This study presents a comparative analysis of the complete genome sequence of the high virulence reference strain Kp52.145, a derivative of the K2 reference strain B5055. It revealed five genomic regions possibly involved in bacterial virulence. One gene, pld1, was shown to be involved in virulence in a mouse model of pneumonia and revealed a novel implication of lipid metabolism in K. pneumoniae pathogenesis. Future analysis of additional putative virulence factors such as Sel1 lipoproteins, VgrG, Hcp, Bcl, cOMP and Sefir-domain containing protein are required for a comprehensive understanding of K. pneumoniae core virulence genes.
Selection of isolates for genome sequencing/bacterial strains
Strain Kp25.145 (a derivative of B5055, the reference strain of serotype K2) is a laboratory strain used to study K. pneumoniae pathogenesis [11, 21] and was chosen as the focus of this work. SB2390 (cur15505, isolated in Curaçao, 2002, urinary tract infection; belongs to ST14) and SB3193 (IPEUC-744, isolated from a metritis case in a mare, 1981 in France; belonging to ST82)  are non-virulent strains that were sequenced to allow the comparison between virulent and non-virulent strains.
The pld1 transposon mutant was isolated by Anna Tomas and Jose Bengoecheoa during a screen of a K. pneumoniae mutant library made by Tn5 transposon insertion (manuscript in preparation). In this mutant, the transposon was inserted at position 1,625 of pld1 gene.
The complementation of the mutant strain was achieved through bacterial transformation using electrocompetent cells and a plasmid carrying pld1 gene. pld1 gene was amplified by PCR and cloned at the multiple cloning site of puc18 plasmid. Cloning was confirmed by DNA sequencing.
Genome sequencing and assembly
K. pneumoniae strains were sequenced using a combination of 454 and Illumina reads. Single and paired-end 454 reads with an average of 400 nucleotides were assembled into contigs and scaffolds by Newbler. Illumina reads of about 76 or 36 nucleotides were aligned to scaffolds in order to confirm and correct possible homopolymer errors in the 454 reads. Coverage was as follows: Kp52.145 genome: 170 X using GAIIX (76 nt) + 13.8 X using MP titanium + 18 X using SR titanium; SB2390 genome: 81 X using GAIIX (36 nt) + 6.4 X using MP titanium + 22 X using SR titanium; SB3193 genome: 209 X using GAIIX (76 nt) + 5.4 X using MP titanium + 20 X using SR titanium.
Following the primary assembly of the genomes, an in silico finishing approach, based on the methods described by Pop et al. , was performed in order to identify small and identical repeats on the genomes. In such cases and without high quality bases discrepancies, the contigs containing the small repeats were manually duplicated and added to the assembly.
Scaffolds were aligned to experimentally determined BglII optical maps generated by OpGen company from purified chromosomal DNA, using MapSolver version 3.1 software. Such alignments were used to check for the quality of the assemblies. Additionally, specific pairs of primers were designed in order to close all remaining gaps. PCR products were purified in NucleoFast 96 plates (Macherey Nagel, Düren, Germany) and aliquots were used for sequencing reactions with the BigDye Terminator Cycle Sequencing Ready reaction Kit (Applied Biosystems, Foster City, CA, USA) on a ABI Prism 3730XL DNA Analyzer (Applied Biosystems). The resulting sequences were added to the previous assembly using Phred/Phrap/Consed.
The sequences of K. pneumoniae genomes have been deposited to the European Nucleotide Archive and are accessible under the accession numbers: FO834904, FO834905 and FO834906 (strain Kp52.145), CCBO00000000 (strain SB2390) and CCCQ000000000 (strain SB3193).
Functional annotation of genomes
In order to gain functional insights about the genome sequences, protein-coding genes were predicted and annotated using the CAAT-box genome browser , using a combination of GeneMark predictions and Blastx results against the Uniprot database. All the putative open reading frames (ORF) longer than 120 nucleotides presenting similarity to sequences of the Uniprot database or positive GeneMark result were considered for further analysis. The final set of CDSs underwent a manual annotation process based on description of similarity. Pfam and COG database searches, as well as SignalP, TMHMM and PredTMBB predictions were performed to improve the degree of annotation confidence, if necessary. CDSs were described as ‘highly similar to’ , ‘similar to’ or ‘weakly similar to’ if they presented more than 70%, between 50% and 69% or less than 50% similarity to the protein hit sequence. Additionally, information on partial homology was included. The start codon for each CDS was automatically chosen and manually validated, based on a combination of GeneMark results and Blast alignments. RAST was used to classify proteins in functional categories. Structural RNAs were searched using tRNAscan.
The common genome of the five strains was determined using the BlastClust algorithm  using minimum parameters of 90% identity and 80% length coverage for proteins to be included in the same cluster.
Lungs of control or Kp52.145-infected mice were homogenized in cold TRI reagent (Sigma, Gillingham, Dorset, UK) using a Precellys lysing kit (Precellys, Saint Quentin en Yvelines, France). Total lung and bacterial mRNAs were extracted according to the manufacturer’s instructions. RNA (2 μg) was reversed transcribed in cDNA using Superscript II enzyme (Invitrogen, Foster City, CA, USA). Aliquots were used in PCR reactions using specific primers [see Additional file 1].
BALB/cJ mice were purchased from Janvier (Le Genest St. Isle, France). Mice were housed under standard conditions of feeding, light and temperature with access to food and water ad libidium. Experiments were performed according to the national and Institut Pasteur guidelines for laboratory animal experiments. Protocols were approved by the Institut Pasteur animal care and use committee (protocol 05–59) and the Direction des Services Vétérinaire de Paris (permit 75–713 to RT). Six- to eight-week-old mice were anesthetized with acepromazine (Calmivet, 1.5 mg/kg, Vetoquinol, Lure, France). and ketamine (Imalgene, 31.25 mg/kg, Merial, Lyon, France). and then infected intranasally with 20 μl bacterial suspension. The virulence of K. pneumoniae strains was tested on six-week-old BALB/c mice, as previously described . Seven mice per test condition were infected with 108 bacteria. Mice were followed every day for one week. Experiments were performed at least twice.
Thin-layer chromatography and mass spectrometry of lipids
Bacterial lipids were extracted by the method of Bligh and Dyer . Briefly, bacterial stationary-phase cultures were concentrated 10 times and mixed with chloroform:methanol. After centrifugation at 1,000 rpm for five minutes, the organic phase was recovered. Lipid profiles were analyzed by two-dimensional TLC using TLC Silica gel 60 F254 plates (Merck, Whitehouse Station, NJ, USA). as the stationary phase and a chloroform 9:1 methanol mixture as the mobile phase in both dimensions. Staining was performed by iodine vapor. TLC calibrated images were aquired in ImageScanner using LabScan v5.0 software. The relative intensity of each spot was calculated in ImageMaster two-dimensional Platinum v7.0 software. Alternatively, lipid extracts were analyzed by MS and MS/MS in an ESI-Q-Tof Micro (Waters), in positive ion mode. Resolution was typically lower than 10 ppm.
Anti-bacterial competition assays
Competition assays were performed as previously described . Briefly, K. pneumoniae Kp52.145 or its pld1 mutant cells grown overnight on an agar plate were resuspended in LB, normalized to OD600 of 0.5 and mixed at a ratio of 5:1 with a spontaneous nalidixic acid (nal) resistant mutant strain of E. coli MG1655. The mixture was incubated for four hours on a prewarmed agar plate. Recovered cells were plated out on antibiotic selective media and viable cells were reported as the total number recovered per co-culture spot. Serratia marcescens DB10 was used as a positive control.
LMSL, AT and this work were supported by an ERA-Net Pathogenomic 2008 grant allocated to both P. Sansonetti and J. Bengoechea. ASA is a recipient of a fellowship from the Fundação para a Ciência e a Tecnologia from Portugal. The authors thank Stephane Salamone and David Touboul for their help with lipid analysis.
- Brisse S, Grimont F, Grimont PAD: The genus Klebsiella. The Prokaryotes: A Handbook on the Biology of Bacteria. 2006, New York: Springer NY, 6: 159-196. 3View ArticleGoogle Scholar
- Keynan Y, Rubinstein E: The changing face of Klebsiella pneumoniae infections in the community. Int J Antimicrob Agents. 2007, 30: 385-389. 10.1016/j.ijantimicag.2007.06.019.PubMedView ArticleGoogle Scholar
- Podschun R, Ullmann U: Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev. 1998, 11: 589-603.PubMed CentralPubMedGoogle Scholar
- Moellering RC: NDM-1–a cause for worldwide concern. N Engl J Med. 2010, 363: 2377-2379. 10.1056/NEJMp1011715.PubMedView ArticleGoogle Scholar
- Shon AS, Russo TA: Hypervirulent Klebsiella pneumoniae: the next superbug?. Future Microbiol. 2012, 7: 669-671. 10.2217/fmb.12.43.PubMedView ArticleGoogle Scholar
- Su SC, Siu LK, Ma L, Yeh KM, Fung CP, Lin JC, Chang FY: Community-acquired liver abscess caused by serotype K1 Klebsiella pneumoniae with CTX-M-15-type extended-spectrum beta-lactamase. Antimicrob Agents Chemother. 2008, 52: 804-805. 10.1128/AAC.01269-07.PubMed CentralPubMedView ArticleGoogle Scholar
- Siu LK, Yeh KM, Lin JC, Fung CP, Chang FY: Klebsiella pneumoniae liver abscess: a new invasive syndrome. Lancet Infect Dis. 2012, 12: 881-887. 10.1016/S1473-3099(12)70205-0.PubMedView ArticleGoogle Scholar
- Yoon JH, Kim YJ, Jun YH, Kim SI, Kang JY, Suk KT, Kim DJ: Liver abscess due to Klebsiella pneumoniae: Risk factors for metastatic infection. Scand J Infect Dis. 2014, 46: 21-26. 10.3109/00365548.2013.851414.PubMedView ArticleGoogle Scholar
- Decre D, Verdet C, Emirian A, Le Gourrierec T, Petit JC, Offenstadt G, Maury E, Brisse S, Arlet G: Emerging severe and fatal infections due to Klebsiella pneumoniae in two university hospitals in France. J Clin Microbiol. 2011, 49: 3012-3014. 10.1128/JCM.00676-11.PubMed CentralPubMedView ArticleGoogle Scholar
- Nassif X, Honore N, Vasselon T, Cole ST, Sansonetti PJ: Positive control of colanic acid synthesis in Escherichia coli by rmpA and rmpB, two virulence-plasmid genes of Klebsiella pneumoniae. Mol Microbiol. 1989, 3: 1349-1359. 10.1111/j.1365-2958.1989.tb00116.x.PubMedView ArticleGoogle Scholar
- Nassif X, Fournier JM, Arondel J, Sansonetti PJ: Mucoid phenotype of Klebsiella pneumoniae is a plasmid-encoded virulence factor. Infect Immun. 1989, 57: 546-552.PubMed CentralPubMedGoogle Scholar
- Regue M, Hita B, Pique N, Izquierdo L, Merino S, Fresno S, Benedi VJ, Tomas JM: A gene, uge, is essential for Klebsiella pneumoniae virulence. Infect Immun. 2004, 72: 54-61. 10.1128/IAI.72.1.54-61.2004.PubMed CentralPubMedView ArticleGoogle Scholar
- Mey A, Ponard D, Colomb M, Normier G, Binz H, Revillard JP: Acylation of the lipid A region of a Klebsiella pneumoniae LPS controls the alternative pathway activation of human complement. Mol Immunol. 1994, 31: 1239-1246. 10.1016/0161-5890(94)90074-4.PubMedView ArticleGoogle Scholar
- Izquierdo L, Coderch N, Pique N, Bedini E, Corsaro MM, Merino S, Fresno S, Tomas JM, Regue M: The Klebsiella pneumoniae wabG gene: role in biosynthesis of the core lipopolysaccharide and virulence. J Bacteriol. 2003, 185: 7213-7221. 10.1128/JB.185.24.7213-7221.2003.PubMed CentralPubMedView ArticleGoogle Scholar
- Llobet E, Tomas JM, Bengoechea JA: Capsule polysaccharide is a bacterial decoy for antimicrobial peptides. Microbiology. 2008, 154: 3877-3886. 10.1099/mic.0.2008/022301-0.PubMedView ArticleGoogle Scholar
- Campos MA, Vargas MA, Regueiro V, Llompart CM, Alberti S, Bengoechea JA: Capsule polysaccharide mediates bacterial resistance to antimicrobial peptides. Infect Immun. 2004, 72: 7107-7114. 10.1128/IAI.72.12.7107-7114.2004.PubMed CentralPubMedView ArticleGoogle Scholar
- Moranta D, Regueiro V, March C, Llobet E, Margareto J, Larrarte E, Garmendia J, Bengoechea JA: Klebsiella pneumoniae capsule polysaccharide impedes the expression of beta-defensins by airway epithelial cells. Infect Immun. 2010, 78: 1135-1146. 10.1128/IAI.00940-09.PubMed CentralPubMedView ArticleGoogle Scholar
- Yeh KM, Kurup A, Siu LK, Koh YL, Fung CP, Lin JC, Chen TL, Chang FY, Koh TH: Capsular serotype K1 or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess in Singapore and Taiwan. J Clin Microbiol. 2007, 45: 466-471. 10.1128/JCM.01150-06.PubMed CentralPubMedView ArticleGoogle Scholar
- Mizuta K, Ohta M, Mori M, Hasegawa T, Nakashima I, Kato N: Virulence for mice of Klebsiella strains belonging to the O1 group: relationship to their capsular (K) types. Infect Immun. 1983, 40: 56-61.PubMed CentralPubMedGoogle Scholar
- Simoons-Smit AM, Verwey-van Vught AM, Kanis IY, MacLaren DM: Virulence of Klebsiella strains in experimentally induced skin lesions in the mouse. J Med Microbiol. 1984, 17: 67-77. 10.1099/00222615-17-1-67.PubMedView ArticleGoogle Scholar
- Nassif X, Sansonetti PJ: Correlation of the virulence of Klebsiella pneumoniae K1 and K2 with the presence of a plasmid encoding aerobactin. Infect Immun. 1986, 54: 603-608.PubMed CentralPubMedGoogle Scholar
- Fung CP, Chang FY, Lee SC, Hu BS, Kuo BI, Liu CY, Ho M, Siu LK: A global emerging disease of Klebsiella pneumoniae liver abscess: is serotype K1 an important factor for complicated endophthalmitis?. Gut. 2002, 50: 420-424. 10.1136/gut.50.3.420.PubMed CentralPubMedView ArticleGoogle Scholar
- Brisse S, Fevre C, Passet V, Issenhuth-Jeanjean S, Tournebize R, Diancourt L, Grimont P: Virulent clones of Klebsiella pneumoniae: identification and evolutionary scenario based on genomic and phenotypic characterization. PLoS One. 2009, 4: e4982-10.1371/journal.pone.0004982.PubMed CentralPubMedView ArticleGoogle Scholar
- Wu KM, Li LH, Yan JJ, Tsao N, Liao TL, Tsai HC, Fung CP, Chen HJ, Liu YM, Wang JT, Fang CT, Chang SC, Shu HY, Liu TT, Chen YT, Shiau YR, Lauderdale TL, Su IJ, Kirby R, Tsai SF: Genome sequencing and comparative analysis of Klebsiella pneumoniae NTUH-K2044, a strain causing liver abscess and meningitis. J Bacteriol. 2009, 191: 4492-4501. 10.1128/JB.00315-09.PubMed CentralPubMedView ArticleGoogle Scholar
- Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, Meyer F, Olsen GJ, Olson R, Osterman AL, Overbeek RA, McNeil LK, Paarmann D, Paczian T, Parrello B, Pusch GD, Reich C, Stevens R, Vassieva O, Vonstein V, Wilke A, Zagnitko O: The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008, 9: 75-10.1186/1471-2164-9-75.PubMed CentralPubMedView ArticleGoogle Scholar
- Di Martino P, Cafferini N, Joly B, Darfeuille-Michaud A: Klebsiella pneumoniae type 3 pili facilitate adherence and biofilm formation on abiotic surfaces. Res Microbiol. 2003, 154: 9-16. 10.1016/S0923-2508(02)00004-9.PubMedView ArticleGoogle Scholar
- Lavender HF, Jagnow JR, Clegg S: Biofilm formation in vitro and virulence in vivo of mutants of Klebsiella pneumoniae. Infect Immun. 2004, 72: 4888-4890. 10.1128/IAI.72.8.4888-4890.2004.PubMed CentralPubMedView ArticleGoogle Scholar
- Balestrino D, Haagensen JA, Rich C, Forestier C: Characterization of type 2 quorum sensing in Klebsiella pneumoniae and relationship with biofilm formation. J Bacteriol. 2005, 187: 2870-2880. 10.1128/JB.187.8.2870-2880.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Agladze K, Wang X, Romeo T: Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA. J Bacteriol. 2005, 187: 8237-8246. 10.1128/JB.187.24.8237-8246.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Torres AG, Jeter C, Langley W, Matthysse AG: Differential binding of Escherichia coli O157:H7 to alfalfa, human epithelial cells, and plastic is mediated by a variety of surface structures. Appl Environ Microbiol. 2005, 71: 8008-8015. 10.1128/AEM.71.12.8008-8015.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Teplitski M, Al-Agely A, Ahmer BM: Contribution of the SirA regulon to biofilm formation in Salmonella enterica serovar Typhimurium. Microbiology. 2006, 152: 3411-3424. 10.1099/mic.0.29118-0.PubMedView ArticleGoogle Scholar
- Suzuki K, Wang X, Weilbacher T, Pernestig AK, Melefors O, Georgellis D, Babitzke P, Romeo T: Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli. J Bacteriol. 2002, 184: 5130-5140. 10.1128/JB.184.18.5130-5140.2002.PubMed CentralPubMedView ArticleGoogle Scholar
- Khan MA, Isaacson RE: Identification of Escherichia coli genes that are specifically expressed in a murine model of septicemic infection. Infect Immun. 2002, 70: 3404-3412. 10.1128/IAI.70.7.3404-3412.2002.PubMed CentralPubMedView ArticleGoogle Scholar
- Baldi DL, Higginson EE, Hocking DM, Praszkier J, Cavaliere R, James CE, Bennett-Wood V, Azzopardi KI, Turnbull L, Lithgow T, Robins-Browne RM, Whitchurch CB, Tauschek M: The Type II secretion system and its ubiquitous lipoprotein substrate, SslE, are required for biofilm formation and virulence of enteropathogenic Escherichia coli. Infect Immun. 2012, 80: 2042-2052. 10.1128/IAI.06160-11.PubMed CentralPubMedView ArticleGoogle Scholar
- Iwobi A, Heesemann J, Garcia E, Igwe E, Noelting C, Rakin A: Novel virulence-associated type II secretion system unique to high-pathogenicity Yersinia enterocolitica. Infect Immun. 2003, 71: 1872-1879. 10.1128/IAI.71.4.1872-1879.2003.PubMed CentralPubMedView ArticleGoogle Scholar
- Hytonen J, Haataja S, Finne J: Streptococcus pyogenes glycoprotein-binding strepadhesin activity is mediated by a surface-associated carbohydrate-degrading enzyme, pullulanase. Infect Immun. 2003, 71: 784-793. 10.1128/IAI.71.2.784-793.2003.PubMed CentralPubMedView ArticleGoogle Scholar
- Shelburne SA, Keith DB, Davenport MT, Beres SB, Carroll RK, Musser JM: Contribution of AmyA, an extracellular alpha-glucan degrading enzyme, to group A streptococcal host-pathogen interaction. Mol Microbiol. 2009, 74: 159-174. 10.1111/j.1365-2958.2009.06858.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Sarris PF, Zoumadakis C, Panopoulos NJ, Scoulica EV: Distribution of the putative type VI secretion system core genes in Klebsiella spp. Infect Genet Evol. 2011, 11: 157-166. 10.1016/j.meegid.2010.09.006.PubMedView ArticleGoogle Scholar
- Lawlor MS, Hsu J, Rick PD, Miller VL: Identification of Klebsiella pneumoniae virulence determinants using an intranasal infection model. Mol Microbiol. 2005, 58: 1054-1073. 10.1111/j.1365-2958.2005.04918.x.PubMedView ArticleGoogle Scholar
- Ge Z, Feng Y, Dangler CA, Xu S, Taylor NS, Fox JG: Fumarate reductase is essential for Helicobacter pylori colonization of the mouse stomach. Microb Pathog. 2000, 29: 279-287. 10.1006/mpat.2000.0391.PubMedView ArticleGoogle Scholar
- Li AH, Lam WL, Stokes RW: Characterization of genes differentially expressed within macrophages by virulent and attenuated Mycobacterium tuberculosis identifies candidate genes involved in intracellular growth. Microbiology. 2008, 154: 2291-2303. 10.1099/mic.0.2008/019661-0.PubMedView ArticleGoogle Scholar
- Buettner FF, Bendallah IM, Bosse JT, Dreckmann K, Nash JH, Langford PR, Gerlach GF: Analysis of the Actinobacillus pleuropneumoniae ArcA regulon identifies fumarate reductase as a determinant of virulence. Infect Immun. 2008, 76: 2284-2295. 10.1128/IAI.01540-07.PubMed CentralPubMedView ArticleGoogle Scholar
- Mercado-Lubo R, Gauger EJ, Leatham MP, Conway T, Cohen PS: A Salmonella enterica serovar typhimurium succinate dehydrogenase/fumarate reductase double mutant is avirulent and immunogenic in BALB/c mice. Infect Immun. 2008, 76: 1128-1134. 10.1128/IAI.01226-07.PubMed CentralPubMedView ArticleGoogle Scholar
- Wu MC, Lin TL, Hsieh PF, Yang HC, Wang JT: Isolation of genes involved in biofilm formation of a Klebsiella pneumoniae strain causing pyogenic liver abscess. PLoS One. 2011, 6: e23500-10.1371/journal.pone.0023500.PubMed CentralPubMedView ArticleGoogle Scholar
- Flower DR: The lipocalin protein family: structure and function. Biochem J. 1996, 318: 1-14.PubMed CentralPubMedView ArticleGoogle Scholar
- Bachman MA, Lenio S, Schmidt L, Oyler JE, Weiser JN: Interaction of lipocalin 2, transferrin, and siderophores determines the replicative niche of Klebsiella pneumoniae during pneumonia. MBio. 2012, 3: 6-View ArticleGoogle Scholar
- Warszawska JM, Gawish R, Sharif O, Sigel S, Doninger B, Lakovits K, Mesteri I, Nairz M, Boon L, Spiel A, Fuhrmann V, Strobl B, Muller M, Schenk P, Weiss G, Knapp S: Lipocalin 2 deactivates macrophages and worsens pneumococcal pneumonia outcomes. J Clin Invest. 2013, 123: 3363-3372. 10.1172/JCI67911.PubMed CentralPubMedView ArticleGoogle Scholar
- Chen YL, Montedonico AE, Kauffman S, Dunlap JR, Menn FM, Reynolds TB: Phosphatidylserine synthase and phosphatidylserine decarboxylase are essential for cell wall integrity and virulence in Candida albicans. Mol Microbiol. 2010, 75: 1112-1132. 10.1111/j.1365-2958.2009.07018.x.PubMedView ArticleGoogle Scholar
- Conde-Alvarez R, Grillo MJ, Salcedo SP, de Miguel MJ, Fugier E, Gorvel JP, Moriyon I, Iriarte M: Synthesis of phosphatidylcholine, a typical eukaryotic phospholipid, is necessary for full virulence of the intracellular bacterial parasite Brucella abortus. Cell Microbiol. 2006, 8: 1322-1335. 10.1111/j.1462-5822.2006.00712.x.PubMedView ArticleGoogle Scholar
- Tang HL, Chiang MK, Liou WJ, Chen YT, Peng HL, Chiou CS, Liu KS, Lu MC, Tung KC, Lai YC: Correlation between Klebsiella pneumoniae carrying pLVPK-derived loci and abscess formation. Eur J Clin Microbiol Infect Dis. 2010, 29: 689-698. 10.1007/s10096-010-0915-1.PubMedView ArticleGoogle Scholar
- Wacharotayankun R, Arakawa Y, Ohta M, Tanaka K, Akashi T, Mori M, Kato N: Enhancement of extracapsular polysaccharide synthesis in Klebsiella pneumoniae by RmpA2, which shows homology to NtrC and FixJ. Infect Immun. 1993, 61: 3164-3174.PubMed CentralPubMedGoogle Scholar
- Hsu CR, Lin TL, Chen YC, Chou HC, Wang JT: The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited. Microbiology. 2011, 157: 3446-3457. 10.1099/mic.0.050336-0.PubMedView ArticleGoogle Scholar
- Hochhut B, Dobrindt U, Hacker J: Pathogenicity islands and their role in bacterial virulence and survival. Contrib Microbiol. 2005, 12: 234-254.PubMedView ArticleGoogle Scholar
- Gal-Mor O, Finlay BB: Pathogenicity islands: a molecular toolbox for bacterial virulence. Cell Microbiol. 2006, 8: 1707-1719. 10.1111/j.1462-5822.2006.00794.x.PubMedView ArticleGoogle Scholar
- Putze J, Hennequin C, Nougayrede JP, Zhang W, Homburg S, Karch H, Bringer MA, Fayolle C, Carniel E, Rabsch W, Oelschlaeger TA, Oswald E, Forestier C, Hacker J, Dobrindt U: Genetic structure and distribution of the colibactin genomic island among members of the family Enterobacteriaceae. Infect Immun. 2009, 77: 4696-4703. 10.1128/IAI.00522-09.PubMed CentralPubMedView ArticleGoogle Scholar
- Lin TL, Lee CZ, Hsieh PF, Tsai SF, Wang JT: Characterization of integrative and conjugative element ICEKp1-associated genomic heterogeneity in a Klebsiella pneumoniae strain isolated from a primary liver abscess. J Bacteriol. 2008, 190: 515-526. 10.1128/JB.01219-07.PubMed CentralPubMedView ArticleGoogle Scholar
- Tsugawa H, Ogawa A, Takehara S, Kimura M, Okawa Y: Primary structure and function of a cytotoxic outer-membrane protein (ComP) of Plesiomonas shigelloides. FEMS Microbiol Lett. 2008, 281: 10-16. 10.1111/j.1574-6968.2007.01041.x.PubMedView ArticleGoogle Scholar
- Shah P, Nanduri B, Swiatlo E, Ma Y, Pendarvis K: Polyamine biosynthesis and transport mechanisms are crucial for fitness and pathogenesis of Streptococcus pneumoniae. Microbiology. 2011, 157: 504-515. 10.1099/mic.0.042564-0.PubMedView ArticleGoogle Scholar
- Wortham BW, Oliveira MA, Fetherston JD, Perry RD: Polyamines are required for the expression of key Hms proteins important for Yersinia pestis biofilm formation. Environ Microbiol. 2010, 12: 2034-2047. 10.1111/j.1462-2920.2010.02219.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Choy HA, Kelley MM, Chen TL, Moller AK, Matsunaga J, Haake DA: Physiological osmotic induction of Leptospira interrogans adhesion: LigA and LigB bind extracellular matrix proteins and fibrinogen. Infect Immun. 2007, 75: 2441-2450. 10.1128/IAI.01635-06.PubMed CentralPubMedView ArticleGoogle Scholar
- Castiblanco-Valencia MM, Fraga TR, Silva LB, Monaris D, Abreu PA, Strobel S, Jozsi M, Isaac L, Barbosa AS: Leptospiral immunoglobulin-like proteins interact with human complement regulators factor H, FHL-1, FHR-1, and C4BP. J Infect Dis. 2012, 205: 995-1004. 10.1093/infdis/jir875.PubMedView ArticleGoogle Scholar
- Beiter K, Wartha F, Albiger B, Normark S, Zychlinsky A, Henriques-Normark B: An endonuclease allows Streptococcus pneumoniae to escape from neutrophil extracellular traps. Curr Biol. 2006, 16: 401-407. 10.1016/j.cub.2006.01.056.PubMedView ArticleGoogle Scholar
- Wu B, Gong J, Liu L, Li T, Wei T, Bai Z: Evolution of prokaryotic homologues of the eukaryotic SEFIR protein domain. Gene. 2012, 492: 160-166. 10.1016/j.gene.2011.10.033.PubMedView ArticleGoogle Scholar
- Ye P, Garvey PB, Zhang P, Nelson S, Bagby G, Summer WR, Schwarzenberger P, Shellito JE, Kolls JK: Interleukin-17 and lung host defense against Klebsiella pneumoniae infection. Am J Respir Cell Mol Biol. 2001, 25: 335-340. 10.1165/ajrcmb.25.3.4424.PubMedView ArticleGoogle Scholar
- Bialek-Davenet S, Nicolas-Chanoine MH, Decre D, Brisse S: Microbiological and clinical characteristics of bacteraemia caused by the hypermucoviscosity phenotype of Klebsiella pneumoniae in Korea. Epidemiol Infect. 2013, 2: 141-188.Google Scholar
- Miyata ST, Kitaoka M, Wieteska L, Frech C, Chen N, Pukatzki S: The Vibrio cholerae Type VI secretion system: evaluating its role in the human disease cholera. Front Microbiol. 2010, 1: 117-PubMed CentralPubMedView ArticleGoogle Scholar
- Shrivastava S, Mande SS: Identification and functional characterization of gene components of Type VI secretion system in bacterial genomes. PLoS One. 2008, 3: e2955-10.1371/journal.pone.0002955.PubMed CentralPubMedView ArticleGoogle Scholar
- Boyer F, Fichant G, Berthod J, Vandenbrouck Y, Attree I: Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources?. BMC Genomics. 2009, 10: 104-10.1186/1471-2164-10-104.PubMed CentralPubMedView ArticleGoogle Scholar
- Newton HJ, Sansom FM, Dao J, McAlister AD, Sloan J, Cianciotto NP, Hartland EL: Sel1 repeat protein LpnE is a Legionella pneumophila virulence determinant that influences vacuolar trafficking. Infect Immun. 2007, 75: 5575-5585. 10.1128/IAI.00443-07.PubMed CentralPubMedView ArticleGoogle Scholar
- McNamara PJ, Bradley GA, Songer JG: Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis. Mol Microbiol. 1994, 12: 921-930. 10.1111/j.1365-2958.1994.tb01080.x.PubMedView ArticleGoogle Scholar
- Hinnebusch J, Cherepanov P, Du Y, Rudolph A, Dixon JD, Schwan T, Forsberg A: Murine toxin of Yersinia pestis shows phospholipase D activity but is not required for virulence in mice. Int J Med Microbiol. 2000, 290: 483-487. 10.1016/S1438-4221(00)80070-3.PubMedView ArticleGoogle Scholar
- McKean SC, Davies JK, Moore RJ: Probing the heat shock response of Corynebacterium pseudotuberculosis: the major virulence factor, phospholipase D, is downregulated at 43 degrees C. Res Microbiol. 2007, 158: 279-286. 10.1016/j.resmic.2006.12.006.PubMedView ArticleGoogle Scholar
- Mohan Das V, Ballal M: Proteinase and phospholipase activity as virulence factors in Candida species isolated from blood. Rev Iberoam Micol. 2008, 25: 208-210. 10.1016/S1130-1406(08)70050-0.PubMedView ArticleGoogle Scholar
- Ponting CP, Kerr ID: A novel family of phospholipase D homologues that includes phospholipid synthases and putative endonucleases: identification of duplicated repeats and potential active site residues. Protein Sci. 1996, 5: 914-922.PubMed CentralPubMedView ArticleGoogle Scholar
- McKean SC, Davies JK, Moore RJ: Expression of phospholipase D, the major virulence factor of Corynebacterium pseudotuberculosis, is regulated by multiple environmental factors and plays a role in macrophage death. Microbiology. 2007, 153: 2203-2211. 10.1099/mic.0.2007/005926-0.PubMedView ArticleGoogle Scholar
- Kuhle K, Flieger A: Legionella phospholipases implicated in virulence. Curr Top Microbiol Immunol. 2013, 376: 175-209.PubMedGoogle Scholar
- Whitworth T, Popov VL, Yu XJ, Walker DH, Bouyer DH: Expression of the Rickettsia prowazekii pld or tlyC gene in Salmonella enterica serovar Typhimurium mediates phagosomal escape. Infect Immun. 2005, 73: 6668-6673. 10.1128/IAI.73.10.6668-6673.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Fevre C, Almeida AS, Taront S, Pedron T, Huerre M, Prevost MC, Kieusseian A, Cumano A, Brisse S, Sansonetti PJ, Tournebize R: A novel murine model of rhinoscleroma identifies Mikulicz cells, the disease signature, as IL-10 dependent derivatives of inflammatory monocytes. EMBO Mol Med. 2013, 5: 516-530. 10.1002/emmm.201202023.PubMed CentralPubMedView ArticleGoogle Scholar
- Shibuya I, Miyazaki C, Ohta A: Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli. J Bacteriol. 1985, 161: 1086-1092.PubMed CentralPubMedGoogle Scholar
- Schlame M: Cardiolipin synthesis for the assembly of bacterial and mitochondrial membranes. J Lipid Res. 2008, 49: 1607-1620. 10.1194/jlr.R700018-JLR200.PubMed CentralPubMedView ArticleGoogle Scholar
- Ray NB, Durairaj L, Chen BB, McVerry BJ, Ryan AJ, Donahoe M, Waltenbaugh AK, O'Donnell CP, Henderson FC, Etscheidt CA, McCoy DM, Agassandian M, Hayes-Rowan EC, Coon TA, Butler PL, Gakhar L, Mathur SN, Sieren JC, Tyurina YY, Kagan VE, McLennan G, Mallampalli RK: Dynamic regulation of cardiolipin by the lipid pump Atp8b1 determines the severity of lung injury in experimental pneumonia. Nat Med. 2010, 16: 1120-1127. 10.1038/nm.2213.PubMed CentralPubMedView ArticleGoogle Scholar
- Lopes SC, Neves CS, Eaton P, Gameiro P: Improved model systems for bacterial membranes from differing species: the importance of varying composition in PE/PG/cardiolipin ternary mixtures. Mol Membr Biol. 2012, 29: 207-217. 10.3109/09687688.2012.700491.PubMedView ArticleGoogle Scholar
- Russell AB, Leroux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD: Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature. 2013, 496: 508-512. 10.1038/nature12074.PubMed CentralPubMedView ArticleGoogle Scholar
- Nagarajan N, Cook C, Di Bonaventura M, Ge H, Richards A, Bishop-Lilly KA, DeSalle R, Read TD, Pop M: Finishing genomes with limited resources: lessons from an ensemble of microbial genomes. BMC Genomics. 2010, 11: 242-10.1186/1471-2164-11-242.PubMed CentralPubMedView ArticleGoogle Scholar
- Frangeul L, Glaser P, Rusniok C, Buchrieser C, Duchaud E, Dehoux P, Kunst F: CAAT-Box, Contigs-Assembly and Annotation Tool-Box for genome sequencing projects. Bioinformatics. 2004, 20: 790-797. 10.1093/bioinformatics/btg490.PubMedView ArticleGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.PubMed CentralPubMedView ArticleGoogle Scholar
- Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959, 37: 911-917. 10.1139/o59-099.PubMedView ArticleGoogle Scholar
- Murdoch SL, Trunk K, English G, Fritsch MJ, Pourkarimi E, Coulthurst SJ: The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors. J Bacteriol. 2011, 193: 6057-6069. 10.1128/JB.05671-11.PubMed CentralPubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.