Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish
BMC Biology volume 8, Article number: 65 (2010)
In mammalian cells, the integrity of the primary cilium is critical for proper regulation of the Hedgehog (Hh) signal transduction pathway. Whether or not this dependence on the primary cilium is a universal feature of vertebrate Hedgehog signalling has remained contentious due, in part, to the apparent divergence of the intracellular transduction pathway between mammals and teleost fish.
Here, using a functional Gli2-GFP fusion protein, we show that, as in mammals, the Gli2 transcription factor localizes to the primary cilia of cells in the zebrafish embryo and that this localization is modulated by the activity of the Hh pathway. Moreover, we show that the Igu/DZIP1protein, previously implicated in the modulation of Gli activity in zebrafish, also localizes to the primary cilium and is required for its proper formation.
Our findings demonstrate a conserved role of the primary cilium in mediating Hedgehog signalling activity across the vertebrate phylum and validate the use of the zebrafish as a representative model for the in vivo analysis of vertebrate Hedgehog signalling.
Signalling by Hedgehog (Hh) family proteins plays a key role in the development ofmany animal species, controlling both cell fate specification and cell proliferation in avariety of contexts. In addition, Hh signalling is deployed to regulate tissue homeostasis post-embryonically and aberrant activity of the Hh pathway has been implicated in anumber of cancers [1, 2]
Most of the components of the Hh signal transduction pathway, including the transmembrane proteins Patched (Ptc) and Smoothened (Smo), the Gli family transcription factor Cubitus interruptus (Ci) and the kinesin-like family protein Cos-2, were first identified by genetic analysis in Drosophila, . Cos-2 binds to Ci and recruits a number of protein kinases including Fused, a positive regulator and Protein kinase A (PKA), a negative regulator of the pathway [4, 5]. PKA phosphorylates Ci, priming it for proteolytic cleavage to yield a truncated form that acts as a transcriptional repressor of Hh target genes. Activation of the pathway disrupts the Cos-2 complex , leading to the accumulation of the full length, activating form of Ci, which enters the nucleus and activates transcription of Hh target genes .
Although the roles of the Ptc, Smo and Gli proteins in Hh signalling appear to have been highly conserved through evolution, several lines of evidence have pointed to a divergence of the intracellular signalling mechanism between flies and vertebrates. In particular, targeted mutation of the murine orthologue of Fused revealed that it is dispensable for Hh signalling in mice [7, 8], while manipulation of the activity of two murine homologues of Cos-2, Kif7 and Kif27, implied that they are similarly not involved in the pathway . At the same time, the discovery that mutations in a number of genes encoding IFT proteins disrupt Hh signalling in the mouse, implicated the primary cilium as a key cellular compartment for Hh signal transduction and led to the suggestion that this unique organelle has subsumed the role of Cos-2 in organizing the intracellular pathway components, at least in mammalian cells . Consistent with this, green fluorescent protein (GFP) tagged forms of the Gli proteins have been shown to localize to the tips of the primary cilia when expressed in primary cultures of mouse limb bud cells .
In the zebrafish, however, analyses based on morpholino mediated knock-down of gene activity indicated that both Fused and Kif7 are required for Hh signalling [12, 13]. This suggests that the pathway in zebrafish is more similar to that in Drosophila and raises doubts about the involvement of the primary cilium in Hh signalling throughout the vertebrates. Moreover, although mutations that disrupt the localization of Smo to thecilium have been shown to cause a loss of Hh pathway activity in zebrafish embryos [14, 15], zebrafish mutant for three of the intraflagellar transport (IFT) protein encoding genes have been reported to show no detectable affect on the activity of the pathway .
Mouse IFT mutants have paradoxical phenotypes suggesting both a partial gain and partial loss of Hh function in different organs. Thus, the IFT88/polaris , IFT57/Hippi and IFT172/wimpole mutants all exhibit polydactyly, indicative of an increase in Hh pathway activity, while at the same time manifesting a loss of Shh-dependent neurons in the neural tube [17, 18]. One way of rationalizing these opposing effects is to posit an overall reduction in the levels of Gli proteins in the mutants, the different consequences reflecting the differing contributions of the activator (Gli-A) and repressor (Gli-R) forms in different tissues. Thus, in the limb bud, where Shh acts principally to de-repress target genes by lowering Gli-R levels, a diminution of Gli-R should lead to a gain of function phenotype. However, in the neural tube, where Shh acts principally to activate target gene transcription by enhancing Gli-A levels, any reduction in these levels should result in a loss of function phenotype.
Zebrafish embryos homozygous for mutant alleles of the iguana (igu) locus exhibit a similarly paradoxical Hh phenotype. In this case they manifest as a gain of pathway activity in the myotome and a loss of pathway activity in the neural tube [19, 20]. Based on an analysis of the igu phenotype and the apparent sub-cellular distribution of a GFP-Igu fusion protein, it was proposed that Igu regulates the nucleo-cytoplasmic shuttling of Gli-A and Gli-R proteins in some manner [19, 20].
In order to investigate the role of Iguana/DZIP1 further, we have generated a transgene encoding a GFP-tagged form of the Gli2a protein and used this to analyse its sub-cellular distribution in wild-type and mutant embryos. We find that, as in mammalian cells, Gli2a localizes to the primary cilia of cells in zebrafish embryos and that this localization is modulated in response to Hh signalling. Analysis of Gli2a localization reveals a prominent defect in primary cilia in igu mutant embryos, implicating Igu/DZIP1 function in primary ciliogenesis. Taken together, our data suggest that the role of the primary cilium as a centre for processing Gli proteins in response to Hh signalling is conserved throughout the vertebrates.
A functional GFP-tagged form of Gli2a localizes to the primary cilium in zebrafish embryos
A key process in the transduction of the Hh signal in all organisms is the regulation of the cleavage and nuclear accumulation of the Gli transcription factors. A major obstacle to the analysis of this process in the zebrafish has been the paucity of reagents with which to detect the various forms of the different Gli proteins. In order partially to circumvent this limitation, we developed a GFP-tagged form of the zebrafish Gli2a protein, which is encoded by the gene inactivated by the yot mutation . We identified a BAC containing the entire Gli2a transcription unit and flanking 5' and 3' sequences and used recombineering  to insert the GFP coding sequence in frame at the C-terminus end of the Gli2a open reading frame such that the full length (Gli-A) form of the protein, but not the truncated (Gli-R) form, should be tagged with GFP. In order to test the function of this tagged protein, we injected the modified BAC into yot mutant embryos. The ability of the tagged protein to rescue the effects caused by mutation of the endogenous gene was assayed by staining the embryos with the mAb F59 in order to detect slow type muscle fibres in the developing myotome. In wild type embryos, F59 accumulates specifically in adaxial cells in response to Hh signalling (Figure 1a) [23, 24] and this response is blocked in yot homozygous embryos which are therefore devoid of adaxial F59 expression (Figure 1b). We found that F59 expression is restored in injected yot mutant embryos, specifically in cells expressing the GFP-tagged protein (Figure 1c). Thus, we conclude that the full-length GFP-tagged Gli2a protein is functional.
As well as the anticipated accumulation of the Gli2a-GFP fusion protein in nuclei, we also observed discrete puncta of GFP signal in expressing cells. A closer examination suggested these to be associated with primary cilia. In order to confirm this, we double stained transiently transformed embryos with antibodies specific for acetylated tubulin or γ-tubulin which label the axonemes and basal bodies of primary cilia, respectively. This showed that the Gli2a-GFP protein was excluded from the basal bodies (Figure 2a') but localized to the distal tip of the primary cilia (Figure 2a). In order to exclude the possibility that this localization is mediated by the GFP moiety in the fusion protein, we analysed the distribution of GFP expressed in transiently transgenic zebrafish embryos and found no evidence of its localization to primary cilia (Additional file 1, Supplementary Figure 1.)
As a result of the mosaic nature of the transgene expression, we analysed its expression in individual cells in a number of embryos and pooled the data. In a sample of 87 Gli2a-GFP expressing cells, we found that the fusion protein localized to the distal tip of the primary cilia in the majority (64%) of cases. In about one third of these (29/87) the protein also localized to the nucleus with varying levels of intensity, but only in rare cases (2/87) was there signal in the cytoplasm in addition to the tip of the cilia. In a second sample of 83 cells showing ciliary localization of the fusion protein, we found 15 cases with low-level expression at the base of the primary cilia in addition to strong expression at the tip.
The localization of Gli2a-GFP to primary cilia is modulated by Hh pathway activity
In order to investigate whether the cilial localization of Gli2a is modulated by Hh pathway activity, we next analysed the distribution of the Gli2a-GFP fusion protein in Hh pathway mutant embryos transiently transgenic for TgBAC(gli2a-GFP). Ptc proteins act both as receptors for the Hh ligand and as repressors of the Hh signal transduction pathway: in the absence of Hh, Ptc inhibits the activity of the signal transducer Smo which, in turn, controls the intracellular balance of Gli-A and Gli-R. Thus, the loss of Ptc function leads to a de-repression of Smo activity and a concomitant increase in Gli-A forms. In embryos doubly homozygous for the ptc1 and ptc2 loss of function alleles , the Gli2a-GFP protein mostly remained localized to the tip of the cilia but showed a higher signal intensity than in wild-type siblings (Figure 2c); Gli2a-GFP was expressed only at the tip of the cilia in most of the cilia examined (74/83) while, in a minority of cases (9/83), diffuse signal was distributed throughout the cilia. In contrast, in homozygous smo mutant embryos [26, 27], the levels of Gli2a-GFP were significantly diminished; in addition, the signal became dispersed along the axoneme or accumulated at both the distal tip and the basal body of the cilium (42/51; Figure 2b). We measured the intensity of the Gli2a-GFP expression at the tip of the cilia in wild type, ptc1;ptc2 mutant and smo mutant embryos and compared its intensity among the three groups. There was a significant difference in the intensity between wild type, smo and ptc1;ptc2 double mutants (Figure 2g-2h). Taken together, these data indicate that the localization of Gli2a to primary cilia is modulated by Hh pathway activity and suggest that, as in mammals, the intracellular transduction of the signal is mediated via this organelle. Interestingly, there was also a significant difference in the length of primary cilia in which Gli2a-GFP was localized compared to those which were unlabelled and between primary cilia in ptc1;ptc2 double mutant embryos compared to those of wild-type embryos (Additional file 2: Supplementary Figure 2).
Gli2a-GFP localization reveals defective primary cilia in iguanamutant zebrafish embryos
Previous analyses of the igu mutant phenotype led to the suggestion that the DZIP protein encoded by the mutated gene regulates the nuclear-cytoplasmic trafficking of the Gli proteins in response to Hh signalling . In order to investigate this proposal further, we generated igu mutant embryos transiently transgenic for TgBAC(gli2a-GFP) and analysed the sub-cellular distribution of the fusion protein. As in wild type siblings, GFP signal could be detected in the nuclei of the igu mutant embryos and also, occasionally, in discreet puncta associated with expressing cells. Staining with anti-acetylated tubulin revealed a co-localization with these puncta. However, in contrast to the elongated axonemes typical of those in wild type embryos, the acetylated tubulin and Gli2a-GFP appeared to coalesce in short stumpy structures that we take to represent vestigial primary cilia (Figure 3b). Consistent with this interpretation, double staining with anti-γ-tubulin revealed these to be associated with basal bodies. However, whereas basal bodies were present in all cells in igu mutant embryos, as in wild type, the incidence of axoneme-like structures was greatly reduced (Figure 3f).
Iguana/DZIP1 is required for primary but not motile cilliogenesis
In contrast to the primary cilia that are present on most cells and have a '9+0' microtubule organization, motile cilia are characterized by a '9+2' microtubule organization, are usually longer than primary cilia and are found only on certain specialized cell types. In zebrafish, motile cilia are present in the pronephros, ventral canal of the spinal cord, the brain and in Kupffer's vesicle where their beating facilitates fluid flow. The loss of these motile cilia results in kidney cysts, hydrocephalus, and left-right asymmetry defects . Motile cilia are also present in the otic vesicle and olfactory sensory neuron [29, 30]. The role of motile kinocilia in the formation of otolith was reported in zebrafish , while in mammals, non-motile olfactory cilia are necessary for detection of the odorants in the environment .
In order to establish whether Igu function is required generally for all cilliogenesis, we examined two prominently ciliated structures, the pronephros and olfactory pits, in igu mutant embryos at 48 hpf and 60 hpf, respectively. In contrast to the truncated primary cilia observed in paraxial mesodermal cells, the motile cilia in the pronephros and olfactory pits appeared to be formed normally in igu mutant embryos (Figure 4b and 4d), although the morphology of the olfactory pits seemed to be somewhat affected. In contrast, motile cilia were absent from the floorplate of igu mutant embryos at 28 hpf (Figure 4f) while those located in Kupffer's vesicle were reduced in number in igu mutant embryos at 10 somite stage (Figure 4h). However, in both cases, the distribution of basal bodies appeared relatively normal (Figure.4i, j, k and 4l). At 48 hpf, however, a few motile cilia were detected in the floorplate of igu mutants (Figure 5b) and by 5 dpf their formation was almost completely recovered (Figure 5c and 5d). Differential interference contrast imaging of the tail region of the floor plate of live igu mutant embryos revealed that these cilia were indeed motile (data not shown). These findings suggest that DZIP1/Igu function is required specifically for the formation of primary cilia and that it is not essential for motile cilia formation. The transcription factor Foxj1a has been shown to be both necessary and sufficient for motile cilia formation and transcription of the foxj1a gene in the early zebrafish embryo is regulated by Hh signalling . The reduction of motile cilia in Kupffer's vesicle and their delayed formation in the floorplate of igu mutant embryos might thus be an indirect consequence of the attenuation of Hh signalling in these structures. Consistent with this interpretation, we found that in contrast to the situation in wild-type embryos, the levels of foxj1a expression in Kupffer's vesicle at the end of gastrulation (Figure 5e and 5f) and in the floorplate before 24 hpf (Figure 5g-j) were reduced relative to those in the pronephros. In order to test the inference that this reduced expression underlies the delay in motile cilium differentiation, we used a heat shock inducible form of foxj1a to drive its expression in igu mutant embryos. Such expression effectively rescued the differentiation of motile cilia in the floorplate of igu mutants (Figure 6d) and also induced ectopic cilia in myotomal cells (Figure 6b), as has previously been described to occur in wild type embryos .
The Iguana/DZIP1 protein localizes to nuclei and to the basal body of primary cilia
In an earlier study, the sub-cellular distribution of the Igu/DZIP1 protein was analysed using a GFP fusion protein encoded by an mRNA injected into newly fertilised embryos . Following fixation of injected embryos, the protein was found to localize predominantly to the cytoplasm. However, co-injection of an mRNA encoding a dominant negative form of the PKA regulatory sub-unit (dnPKA; which activates Hh target gene expression by abrogating proteolytic processing of the full length forms of Gli proteins), was reported to induce a re-localisation of the fusion protein to the nucleus . In the light of our new findings, we repeated these experiments, specifically to address the relationship between Igu/DZIP1 protein localization and its role in ciliogenesis. Inspection of the sequence of the cDNA clone encoding the GFP-Igu fusion protein revealed three nucleotide substitutions that cause a divergence from the published wild type amino acid sequence at three residues. We corrected these substitutions by in vitro mutagenesis (see Materials and Methods) and injected the modified mRNA into embryos derived from igu heterozygous parents. Homozygous mutant embryos thus injected showed a complete rescue to wild type morphology (confirmed by genotyping the injected mutant embryos). We observed discrete puncta of GFP signal in each cell, which we surmised represented localization to the primary cilia. In order to confirm this, we fixed injected embryos and stained them with anti-acetylated tubulin and γ-tubulin. This revealed that Igu protein is excluded from the axoneme (Figure 7a) and co-localizes with basal bodies (data not shown). We also visualised this co-localization in live embryos by simultaneous injection of mRNA encoding the GFP-Igu fusion and a γ-tubulin associated protein 3-tdTomato fusion (Figure 7a'). Co-injection of mRNA encoding dnPKA with the GFP-igu mRNA had no effect on cilia localization of the protein (Figure 7b).
Previous studies have implied a divergence in the mechanisms of Hh signalling between mammals and zebrafish , raising doubts about the role of the primary cilium in Hh signalling in the latter , a view reinforced by the apparent lack of effect of zebrafish IFT mutants on Hh signalling . The recent finding that the role of Kif7 as a regulator of the Hh signal transduction is, in fact, conserved in mice [33–35] has, however, removed a major line of evidence in support of the case for divergence. Our data, together with complementary findings reported recently by Huang and Schier  now provide compelling evidence that Hh signals are indeed transduced in zebrafish, as in mammals, via the primary cilium. Here, we demonstrate that a functional Gli2a-GFP fusion protein localizes to the primary cilia of cells in the zebrafish embryo and that this localization is modulated by the activity of the Hh pathway. These findings are consistent with previous reports that Gli2 localizes to the tips of cilia in mouse limb-bud derived embryonic fibroblasts  and provide the first in vivo evidence for the role of Ptc and Smo in mediating such localization. Moreover, we show that loss of Igu/DZIP1 function, which has previously been demonstrated to cause a de-regulation of Gli activity in zebrafish embryos [19, 20], disrupts the structure of primary cilia. We note that three other papers implicating DZIP1/Igu in primary ciliogenesis have been published since submission of this manuscript [37–39]. In line with these findings, complete ablation of the primary cilia by removal of both zygotic and maternal expression of oval/IFT88 clearly disrupts Hh signalling in the zebrafish embryo, leading to phenotypic effects remarkably similar to those of igu mutants .
By introducing a transgene encoding a Gli2a-GFP fusion protein into mutant embryos, we found that accumulation of the protein at the tip of the primary cilium is promoted by Hh pathway activity. Thus, in embryos doubly mutant for the genes encoding the Hh receptors Ptc1 and Ptc2, the levels of the fusion protein at the tip of the cilia are intensified, whereas in Smo mutants the levels were reduced and the protein was found in the basal bodies as well as along the axoneme. This suggests that in the absence of Hh signalling Gli2 is shuttled between the tip of the cilium and the basal body: the latter is known to be enriched for proteasomes  so may represent the site of processing Gli proteins to their repressor forms. Activation of the pathway results in a localization of the full-length protein at the tip of the cilium, thus removing it from proximity to the processing machinery. The increased length of primary cilia that accumulate Gli2-GFP at their tips or that a lack of functional Ptc1 and Ptc2 proteins was unexpected. However, recent studies have suggested that cilia length is modulated by cAMP levels in response to external stimuli [41, 42]. Our findings provide a hint that Hh signalling may similarly influence the length of primary cilia; exactly how this is effected awaits further investigation.
Epistasis analyses of the igu mutation have previously demonstrated that the induction of ectopic Engrailed-expressing medial fast fibres (MFFs) in the embryonic myotome of mutant embryos depends critically upon Gli1 activity, implying that the loss of DZIP1 function, and hence in the light of our current analysis, of primary cilia, leads to a relative increase in the activity of this transcription factor. Notably, igu mutants were also shown to be resistant to the effects of PKA inhibition [19, 20], which in wild type embryos abrogates the production of the repressor forms of the Gli proteins. The resistance of igu mutants to PKA inhibition implies that PKA does not have a significant impact on Gli activity in the absence of cilia. It follows that in igu mutants, levels of the Gli-R forms will be significantly reduced, leading to an increase in the relative levels of Gli-A forms. While such a shift in the Gli-A/Gli-R balance can account for the expansion of Hh dependent cell types in the myotome, it is notable that cell types dependent upon the highest levels of Hh signalling are reduced or absent from both the myotome and neural tube of igu mutants [19, 20]. This implies that an additional cilia-dependent process is required for the maximal activity of Gli-A forms.
The localization of the GFP-Igu fusion protein to the basal body of the cilium isconsistent with its role in ciliogenesis and mirrors that of IFT proteins  as well as of the chicken Talpid3 protein , mutation of which produces a phenotype strikinglysimilar to that of the zebrafish igu mutant . Like Igu/DZIP1, Talpid3 and several IFT proteins are characterized by the presence of coiled-coil domains, a motif that mediates protein-protein interactions. This shared sub-cellular localization and structural motif suggests that Igu/DZIP1 may be a component of a multi-protein complex.
Although the primary cilia are severely truncated in igu mutant embryos, we find that motile cilia in the pronephric ducts, olfactory pits and neural tube are largely unaffected by the mutation. This implies a specific role for Igu/DZIP1 in primary ciliogenesis, which contrasts with the involvement of IFT proteins in the formation of both primary and motile cilia. We note, however, that some motile cilia are affected in igu mutants, namely those in the floorplate at earlier stages of embryogenesis and in the middle of Kupffer's vesicle. The latter have been implicated in generating the nodal flow that controls left right asymmetry in vertebrate embryos. Hence, their absence could account for the previous observation that L-R asymmetry is disrupted in igu mutant . In the floor plate, motile cilia were observed only at the tip of the tail at 28 hpf. However, by 5 dpf, such cilia had recovered along the length of the neural tube. Our finding that the levels of expression of the gene encoding the Foxj1a transcription factor are reduced specifically in Kupffer's vesicle and floorplate cells in igu mutants is consistent with this effect on motile cilia being an indirect consequence of the attenuation of Hh signal transduction caused by the disruption of the primary cilia formation. That heat shock driven expression of Foxj1a can induce motile cilia formation in igu mutant embryos supports this view. The recovery of these cilia at later stage could reflect a gradual increase in the levels of Gli activity in igu mutants, as implied by the induction of ectopic MFFs in the myotome [19, 20].
Our findings provide further evidence for the role of the primary cilium in the modulation of Gli transcription factor activity in response to Hh signalling and confirm that such a role is conserved from teleosts to mammals. They also suggest a role for Hh signalling in controlling primary cilium length. The localization of the DZIP1 protein to the base of the primary cilium is consistent with its requirement for primary cilium assembly, as revealed by the igu mutant phenotype. The finding that motile cilia are largely unaffected in igu mutants, suggests that, in contrast to IFT proteins, DZIP1 function may be dedicated specifically to primary ciliogenesis.
Zebrafish strains and husbandry
Wild type embryos were obtained from AB or LWT strains. Mutant embryos were obtained from iguts294e[19, 20], ptc1hu1602:ptc2tj222 and smohi1640. Adult fish were maintained on a 14 h light/10 h dark cycle at 28°C in UK Home Office approved facilities in the Medical Research Council Centre for Developmental and Biomedical Genetics aquaria at the University of Sheffield.
Isolation and tagging of the Gli2a BAC
Potential Gli2a-containing BACs were isolated using a bioinformatics approach, initially by blasting the cDNA sequence against all finished and unfinished sequences in theSanger and Ensembl genome databases. The Spidey mRNA to genomic alignmentprogram http://www.ncbi.nlm.nih.gov/spidey/ was then used to analyse exonpresence and order in the sequence of potential BACs. The candidate BACs were thenlocated in the Sanger tiling path and overlapping BACs on either side were also selected in order to cover as much as the Gli2a genomic region as possible. A total of seven BACswere obtained from RZPD (Berlin, Germany). Primers were designed to exons 1, 3, 6 and 13 of the Gli2a cDNA, and the presence of each of these exons in each of the BACs was tested by polymerase chain reaction (PCR) in order to identify a full-length clone. Genomic DNA was used as a positive control. CH211-216K4 was found to contain all of the Gli2a exons. BAC DNA was midi-prepped using the Nucleobond kit. Standard recombineering techniques  were used to generate a transgene encoding a C-terminally GFP-tagged form of Gli2a gene, replacing the stop codon of the Gli2a transcription unit with the start codon of the GFP coding region.
Sequencing of the full-length GFP-igu insert revealed that there were three amino acid substitutions that differed from the published igu cDNA sequence. These were at amino acid positions 65 (F>Y, TTT>TAT), 74 (V>A, GTG>GCG) and 488 (Q>H, CAG>CAT) of the published Genbank sequence.
Primers were designed to correct these errors by PCR:
The construct was then corrected using Stratagene's multi-site directed mutagenesis kit (Agilent Technologies, Cheshire, UK) according to the manufacturer's instructions and confirmed by sequencing. The full-length zebrafish tubulin-γ-complex-associated protein 3 (GTAP3) coding region was PCR amplified from embryonic reverse transcriptase-cDNA and cloned in frame with the ATG of tdTomato into the pCS2 vector.
A full-length zebrafish foxj1a was amplified by PCR and subcloned into pSGH2 vector  which contains 8xHeat shock elements and GFP reporter. The construct was injected into 1-cell stage embryos. The injected embryos were heat shocked at 11 hpf in the PCR machine; 22°C 30 min and 37°C 30 min, two cycles. The embryos were heat shocked again at 21 hpf and incubated at 28°C until fixation at 24 hpf.
RNA in situhybridization
In situ hybridization was performed essentially as previously described . A partial foxj1a was isolated by PCR and a digoxigenin-labelled anti-sense foxj1a probe was prepared from a partial cDNA clone. The clone was linearized with NotI and transcribed with SP6 polymerase (Roche, Hertfordshire, UK).
Immunohistochemistry was performed essentially as previously described . Embryoswere fixed in 4% paraformaldehyde in phosphate buffered saline. Mouse monoclonal anti-F59 (Hybridoma Bank) was used at 1:20 dilution and Mouse monoclonal anti-acetylated tubulin and anti-γ-tubulin (Sigma, Dorset, UK) were used at 1:200 dilution. Anti-mouse secondary antibody Cy3 (Jackson lab) was used at 1:200. Nuclei were labelled using a mounting medium with DAPI (Vectashield, CA, USA).
in vitromRNA transcription
Expression constructs were linearized with NotI and transcribed with SP6 polymerase.The SP6 mMessage Machine kit (Ambion, Texas, USA) was used for all in vitro transcriptions.
Wild type or mutant embryos were injected at the 1-cell stage with BAC DNA or cappedmessenger RNA using a Narishige IM-300 micromanipulators.
Immunolabelled embryos and live embryos (embedded in 1% low melting agarose) wereanalysed using an Olympus FV-1000 confocal microscope.
bacterial artificial chromosome
green fluorescent protein
- Igu :
medial fast twitch muscle fibre
polymerase chain reaction
protein kinase A
- yot you too :
McMahon AP, Ingham PW, Tabin CJ: Developmental roles and clinical significance of hedgehog signaling. Curr Top Dev Biol. 2003, 53: 1-114. full_text.
Jiang J, Hui CC: Hedgehog signaling in development and cancer. Dev Cell. 2008, 15 (6): 801-812. 10.1016/j.devcel.2008.11.010.
Ingham PW, McMahon AP: Hedgehog signaling in animal development: paradigms and principles. Genes Dev. 2001, 15 (23): 3059-3087. 10.1101/gad.938601.
Monnier V, Ho KS, Sanial M, Scott MP, Plessis A: Hedgehog signal transduction proteins: contacts of the Fused kinase and Ci transcription factor with the kinesin-related protein Costal2. BMC Dev Biol. 2002, 2: 4-10.1186/1471-213X-2-4.
Zhang W, Zhao Y, Tong C, Wang G, Wang B, Jia J, Jiang J: Hedgehog-regulated Costal2-kinase complexes control phosphorylation and proteolytic processing of Cubitus interruptus. Dev Cell. 2005, 8 (2): 267-278. 10.1016/j.devcel.2005.01.001.
Ruel L, Gallet A, Raisin S, Truchi A, Staccini-Lavenant L, Cervantes A, Therond PP: Phosphorylation of the atypical kinesin Costal2 by the kinase Fused induces the partial disassembly of the Smoothened-Fused-Costal2-Cubitus interruptus complex in Hedgehog signalling. Development. 2007, 134 (20): 3677-3689. 10.1242/dev.011577.
Chen MH, Gao N, Kawakami T, Chuang PT: Mice deficient in the fused homolog do not exhibit phenotypes indicative of perturbed hedgehog signaling during embryonic development. Mol Cell Biol. 2005, 25 (16): 7042-7053. 10.1128/MCB.25.16.7042-7053.2005.
Chen MH, Wilson CW, Li YJ, Law KK, Lu CS, Gacayan R, Zhang X, Hui CC, Chuang PT: Cilium-independent regulation of Gli protein function by Sufu in Hedgehog signaling is evolutionarily conserved. Genes Dev. 2009, 23 (16): 1910-1928. 10.1101/gad.1794109.
Varjosalo M, Li SP, Taipale J: Divergence of hedgehog signal transduction mechanism between Drosophila and mammals. Dev Cell. 2006, 10 (2): 177-186. 10.1016/j.devcel.2005.12.014.
Huangfu D, Anderson KV: Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates. Development. 2006, 133 (1): 3-14. 10.1242/dev.02169.
Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK: Gli2 and Gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function. PLoS Genet. 2005, 1 (4): e53-10.1371/journal.pgen.0010053.
Wolff C, Roy S, Ingham PW: Multiple muscle cell identities induced by distinct levels and timing of hedgehog activity in the zebrafish embryo. Curr Biol. 2003, 13 (14): 1169-1181. 10.1016/S0960-9822(03)00461-5.
Tay SY, Ingham PW, Roy S: A homologue of the Drosophila kinesin-like protein Costal2 regulates Hedgehog signal transduction in the vertebrate embryo. Development. 2005, 132 (4): 625-634. 10.1242/dev.01606.
Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY, Reiter JF: Vertebrate Smoothened functions at the primary cilium. Nature. 2005, 437 (7061): 1018-1021. 10.1038/nature04117.
Aanstad P, Santos N, Corbit KC, Scherz PJ, le Trinh A, Salvenmoser W, Huisken J, Reiter JF, Stainier DY: The extracellular domain of Smoothened regulates ciliary localization and is required for high-level Hh signaling. Curr Biol. 2009, 19 (12): 1034-1039. 10.1016/j.cub.2009.04.053.
Lunt SC, Haynes T, Perkins BD: Zebrafish ift57, ift88, and ift172 intraflagellar transport mutants disrupt cilia but do not affect hedgehog signaling. Dev Dyn. 2009, 238 (7): 1744-1759. 10.1002/dvdy.21999.
Huangfu D, Liu A, Rakeman AS, Murcia NS, Niswander L, Anderson KV: Hedgehog signalling in the mouse requires intraflagellar transport proteins. Nature. 2003, 426 (6962): 83-87. 10.1038/nature02061.
Liu A, Wang B, Niswander LA: Mouse intraflagellar transport proteins regulate both the activator and repressor functions of Gli transcription factors. Development. 2005, 132 (13): 3103-3111. 10.1242/dev.01894.
Sekimizu K, Nishioka N, Sasaki H, Takeda H, Karlstrom RO, Kawakami A: The zebrafish iguana locus encodes Dzip1, a novel zinc-finger protein required for proper regulation of Hedgehog signaling. Development. 2004, 131 (11): 2521-2532. 10.1242/dev.01059.
Wolff C, Roy S, Lewis KE, Schauerte H, Joerg-Rauch G, Kirn A, Weiler C, Geisler R, Haffter P, Ingham PW: iguana encodes a novel zinc-finger protein with coiled-coil domains essential for Hedgehog signal transduction in the zebrafish embryo. Genes Dev. 2004, 18 (13): 1565-1576. 10.1101/gad.296004.
Karlstrom RO, Talbot WS, Schier AF: Comparative synteny cloning of zebrafish you-too: mutations in the Hedgehog target gli2 affect ventral forebrain patterning. Genes Dev. 1999, 13 (4): 388-393. 10.1101/gad.13.4.388.
Lee EC, Yu D, Martinez de Velasco J, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG: A highly efficient Escherichia c oli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics. 2001, 73 (1): 56-65. 10.1006/geno.2000.6451.
Devoto SH, Melancon E, Eisen JS, Westerfield M: Identification of separate slow and fast muscle precursor cells in vivo, prior to somite formation. Development. 1996, 122 (11): 3371-3380.
Barresi MJ, Stickney HL, Devoto SH: The zebrafish slow-muscle-omitted gene product is required for Hedgehog signal transduction and the development of slow muscle identity. Development. 2000, 127 (10): 2189-2199.
Koudijs MJ, den Broeder MJ, Groot E, van Eeden FJ: Genetic analysis of the two zebrafish patched homologues identifies novel roles for the hedgehog signaling pathway. BMC Dev Biol. 2008, 8: 15-10.1186/1471-213X-8-15.
Chen W, Burgess S, Hopkins N: Analysis of the zebrafish smoothened mutant reveals conserved and divergent functions of hedgehog activity. Development. 2001, 128 (12): 2385-2396.
Varga ZM, Amores A, Lewis KE, Yan YL, Postlethwait JH, Eisen JS, Westerfield M: Zebrafish smoothened functions in ventral neural tube specification and axon tract formation. Development. 2001, 128 (18): 3497-3509.
Kramer-Zucker AG, Olale F, Haycraft CJ, Yoder BK, Schier AF, Drummond IA: Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis. Development. 2005, 132 (8): 1907-1921. 10.1242/dev.01772.
Colantonio JR, Vermot J, Wu D, Langenbacher AD, Fraser S, Chen JN, Hill KL: The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear. Nature. 2009, 457 (7226): 205-209. 10.1038/nature07520.
Pathak N, Obara T, Mangos S, Liu Y, Drummond IA: The zebrafish fleer gene encodes an essential regulator of cilia tubulin polyglutamylation. Mol Biol. 2007, 18 (11): 4353-4364.
McEwen DP, Jenkins PM, Martens JR: Olfactory cilia: our direct neuronal connection to the external world. Curr Top Dev Biol. 2008, 85: 333-370. full_text.
Yu X, Ng CP, Habacher H, Roy S: Foxj1 transcription factors are master regulators of the motile ciliogenic program. Nat Genet. 2008, 40 (12): 1445-1453. 10.1038/ng.263.
Cheung HO, Zhang X, Ribeiro A, Mo R, Makino S, Puviindran V, Law KK, Briscoe J, Hui CC: The kinesin protein Kif7 is a critical regulator of Gli transcription factors in mammalian hedgehog signaling. Sci Signal. 2009, 2 (76): ra29-10.1126/scisignal.2000405.
Liem KF, He M, Ocbina PJ, Anderson KV: Mouse Kif7/Costal2 is a cilia-associated protein that regulates Sonic hedgehog signaling. Proc Natl Acad Sci USA. 2009
Endoh-Yamagami S, Evangelista M, Wilson D, Wen X, Theunissen JW, Phamluong K, Davis M, Scales SJ, Solloway MJ, de Sauvage FJ, Peterson AS: The mammalian Cos2 homolog Kif7 plays an essential role in modulating Hh signal transduction during development. Curr Biol. 2009, 19 (15): 1320-1326. 10.1016/j.cub.2009.06.046.
Huang P, Schier AF: Dampened Hedgehog signaling but normal Wnt signaling in zebrafish without cilia. Development. 2009, 136 (18): 3089-3098. 10.1242/dev.041343.
Rink JC, Gurley KA, Elliott SA, Sanchez Alvarado A: Planarian Hh signaling regulates regeneration polarity and links Hh pathway evolution to cilia. Science. 2009, 326 (5958): 1406-1410. 10.1126/science.1178712.
Glazer AM, Wilkinson AW, Backer CB, Lapan SW, Gutzman JH, Cheeseman IM, Reddien PW: The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis. Dev Biol. 337 (1): 148-156. 10.1016/j.ydbio.2009.10.025.
Tay SY, Yu X, Wong KN, Panse P, Ng CP, Roy S: The iguana/DZIP1 protein is a novel component of the ciliogenic pathway essential for axonemal biogenesis. Dev Dyn. 239 (2): 527-534. 10.1002/dvdy.22199.
Gerdes JM, Liu Y, Zaghloul NA, Leitch CC, Lawson SS, Kato M, Beachy PA, Beales PL, DeMartino GN, Fisher S: Disruption of the basal body compromises proteasomal function and perturbs intracellular Wnt response. Nat Genet. 2007, 39 (11): 1350-1360. 10.1038/ng.2007.12.
Ou Y, Ruan Y, Cheng M, Moser JJ, Rattner JB, Hoorn van der FA: Adenylate cyclase regulates elongation of mammalian primary cilia. Exp Cell Res. 2009, 315 (16): 2802-2817. 10.1016/j.yexcr.2009.06.028.
Besschetnova TY, Kolpakova-Hart E, Guan Y, Zhou J, Olsen BR, Shah JV: Identification of signaling pathways regulating primary cilium length and flow-mediated adaptation. Curr Biol. 20 (2): 182-187. 10.1016/j.cub.2009.11.072.
Jurczyk A, Gromley A, Redick S, San Agustin J, Witman G, Pazour GJ, Peters DJ, Doxsey S: Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly. J Cell Biol. 2004, 166 (5): 637-643. 10.1083/jcb.200405023.
Yin Y, Bangs F, Paton IR, Prescott A, James J, Davey MG, Whitley P, Genikhovich G, Technau U, Burt DW, Tickle C: The Talpid3 gene (KIAA0586) encodes a centrosomal protein that is essential for primary cilia formation. Development. 2009, 136 (4): 655-664. 10.1242/dev.028464.
Lewis KE, Drossopoulou G, Paton IR, Morrice DR, Robertson KE, Burt DW, Ingham PW, Tickle C: Expression of ptc and gli genes in talpid3 suggests bifurcation in Shh pathway. Development. 1999, 126 (11): 2397-2407.
Chen JN, van Eeden FJ, Warren KS, Chin A, Nusslein-Volhard C, Haffter P, Fishman MC: Left-right pattern of cardiac BMP4 may drive asymmetry of the heart in zebrafish. Development. 1997, 124 (21): 4373-4382.
Bajoghli B, Aghaallaei N, Heimbucher T, Czerny T: An artificial promoter construct for heat-inducible misexpression during fish embryogenesis. Dev Biol. 2004, 271 (2): 416-430. 10.1016/j.ydbio.2004.04.006.
Oxtoby E, Jowett T: Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development. Nucleic Acids Res. 1993, 21 (5): 1087-1095. 10.1093/nar/21.5.1087.
We thank Stone Elworthy, Ashish Maurya, Huang Mei, Sudipto Roy and Carl Smythe for their helpful discussions. This work was supported by Wellcome Trust grants 082962 and 074974. The CDBG zebrafish aquarium and confocal microscope are supported by a Medical Research Council (UK) Centre grant. Grant Sponsor: Wellcome Trust, Grant numbers: 082962, 074974.
HRK participated in the experimental design, performed all of the Gli2-GFP localization studies and the igu mutant analysis and participated in drafting the manuscript. JR generated the BAC engineered Gli2-GFP fusion construct and corrected the igu cDNA sequence. FvE generated the ptc1;ptc2 double mutant animals and participated in the discussion of results. PWI conceived of the study, participated in the design and coordination of the experiments and drafted the manuscript. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: Supplementary Figure 1: Green fluorescent protein (GFP) does not localize to primary cilia. In order to confirm that GFP alone does not localize to the primary cilia, we cloned GFP into pCS2 vector and injected into newly fertilised zebrafish eggs. Injected embryos were fixed at 18 hpf. Primary cilia and nuclei were visualised with acetylated tubulin (red) and DAPI (blue) respectively. GFP can be seen to localize to the cytoplasm and nuclei in both slow (b) and fast muscle (a) cells, but was not detected in the primary cilia. Green channels from (a) and (b) are shown in (c) and (d), respectively. Scale bar:10 μm (TIFF 3 MB)
Additional file 2: Supplementary Figure 2. Gli2a-green fluorescent protein (GFP) positive cilia are longer than those lacking Gli2a-GFP protein. We noticed that Gli2a-GFP positive cilia appear longer than other cilia, especially in ptc1;ptc2 double mutant embryos. In order to confirm this, we measured the length of the cilia in both Gli2a-GFP positive and negative cilia in ptc1;ptc2 double mutant and the difference in the mean lengths between the two groups was tested by the paired t- test. The difference was significant with P < 0.001 (a). In wild type embryos, the difference in the length of the cilia between Gli2a-GFP positive and Gli2a-GFP negative cilia was also significant, although not as great as in ptc1;ptc2 double mutant (b). We also compared the length of the Gli2a-GFP positive cilia in wild type and in ptc1;ptc2 double mutants, and again found a significant difference (c). The length of cilia lacking the Gli2a-GFP fusion protein, by contrast, did not differ between in wild type and ptc1;ptc2 mutant embryos. (TIFF 7 MB)
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Kim, H.R., Richardson, J., van Eeden, F. et al. Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish. BMC Biol 8, 65 (2010). https://doi.org/10.1186/1741-7007-8-65
- Basal Body
- Zebrafish Embryo
- Primary Cilium
- Mutant Embryo
- Wild Type Embryo