- Research article
- Open Access
Nuclear myosin 1 contributes to a chromatin landscape compatible with RNA polymerase II transcription activation
© Almuzzaini et al. 2015
- Received: 3 December 2014
- Accepted: 2 June 2015
- Published: 5 June 2015
Nuclear myosin 1c (NM1) is emerging as a regulator of transcription and chromatin organization.
Using chromatin immunoprecipitation and deep sequencing (ChIP-Seq) in combination with molecular analyses, we investigated the global association of NM1 with the mammalian genome. Analysis of the ChIP-Seq data demonstrates that NM1 binds across the entire mammalian genome with occupancy peaks correlating with distributions of RNA Polymerase II (Pol II) and active epigenetic marks at class II gene promoters. In mouse embryonic fibroblasts subjected to RNAi mediated NM1 gene silencing, we show that NM1 synergizes with polymerase-associated actin to maintain active Pol II at the promoter. NM1 also co-localizes with the nucleosome remodeler SNF2h at class II promoters where they assemble together with WSTF as part of the B-WICH complex. A high resolution micrococcal nuclease (MNase) assay and quantitative real time PCR shows that this mechanism is required for local chromatin remodeling. Following B-WICH assembly, NM1 mediates physical recruitment of the histone acetyl transferase PCAF and the histone methyl transferase Set1/Ash2 to maintain and preserve H3K9acetylation and H3K4trimethylation for active transcription.
We propose a novel genome-wide mechanism where myosin synergizes with Pol II-associated actin to link the polymerase machinery with permissive chromatin for transcription activation.
- RNA polymerase II transcription
- Genome-wide analysis
Gene expression programs are activated and repressed via ATP-dependent chromatin remodeling and epigenetic modifications. During spatial and temporal activation of genes these mechanisms target nucleosomes, DNA and histone tails [1, 2], impacting both cellular function and organismal development. By repositioning nucleosomes, ATP-dependent chromatin remodelers contribute to chromatin accessibility and exposure of DNA regulatory elements . At the gene promoter, these mechanisms must be coordinated with a range of histone modifications, including acetylation, methylation, phosphorylation and ubiquitination, to collectively define different gene activity states [4, 5]. Acetylation on K9 of histone H3 (H3K9ac) by histone acetyl transferases (HAT) is commonly found at active promoters and it is therefore referred to as an epigenetic mark for active transcription. Although there are examples of enrichment at other genomic regions [6, 7], H3K4 trimethylation (H3K4me3) by histone methyl transferases (HMTs) is also associated with active chromatin, enriched at both active and poised promoters . One critical histone mark that cooperates with H3K4me3 at active promoters is the modification of H3K27 by acetylation (H3K27ac) . H3K27ac together with the monomethyl state of H3K4 (H3K4me1) also marks active gene enhancers [10–12]. Although both remodeling and histone modifications are essential to open up the chromatin and, thus, regulate accessibility of RNA polymerase to become engaged in active transcription, how recruitment of remodelers and active epigenetic marks is temporally orchestrated and preserved is not fully understood.
Motor proteins such as myosin are emerging as key regulators of chromatin. They coordinate global chromatin dynamics with gene-specific activities and directly affect the functional architecture of the cell nucleus . Among the nuclear myosin species, the myosin 1c isoform B - referred to as nuclear myosin 1 (NM1) - is the best characterized both in terms of location and function [14–21]. NM1 works with actin and nuclear components to regulate different steps in the gene expression pathway [13, 22, 23] and has an impact at the genomic level . NM1 associates with the chromatin and this association is functional since NM1 localizes to both nuclear and nucleolar transcription sites in an RNA-dependent manner [15, 19, 24–26]. At the rRNA gene promoter, the interaction between the chromatin-bound NM1 and the RNA polymerase I (Pol I)-associated actin is required for transcription activation . NM1 is also part of B-WICH, a multiprotein assembly that contains the WICH chromatin remodeling complex with the subunits WSTF and the ATPase SNF2h [19, 27, 28]. On the rDNA we found that WSTF bookmarks the position of the chromatin remodeling complex while NM1 interacts with SNF2h to stabilize B-WICH, leading to recruitment of the HAT PCAF for H3K9 acetylation . NM1 has therefore been proposed to connect Pol I with the rDNA through direct interactions with the Pol I-associated actin and chromatin, respectively. Since this mechanism depends on the myosin ATPase activity and the catalytic activity of NM1 is required for Pol I transcription, NM1 is likely to function as an actin-based motor that activates transcription by providing a permissive chromatin state for rapid Pol I transcription activation [20, 25, 26]. Actin also interacts with unphosphorylated RNA polymerase II (Pol II) as well as hypo- (phospho-S5) and hyperphosphorylated (phospho-S5 and phospho-S2) forms of Pol II [29–31]. There is also in vitro evidence that NM1 plays a role in Pol II transcription at different stages [13, 14, 18]. Whether the chromatin-based mechanisms described above generally apply to Pol II is, however, not known.
We set out to investigate whether NM1 has a fundamental function at class II promoters, contributing to the maintenance of a chromatin state required for Pol II transcription activation. Using chromatin immunoprecipitation and deep sequencing (ChIP-Seq), we show, for the first time, association of a myosin species with a mammalian genome. The results from pairwise comparisons of the genomic distributions of NM1 and SNF2h  show co-localization at multiple genomic locations. Within a subset of these locations NM1, SNF2h and WSTF are enriched at active class II promoters where NM1 specifically maintains hypophosphorylated Pol II levels and modulates B-WICH assembly, including SNF2h recruitment for local chromatin remodeling. We demonstrate that this, in turn, leads to recruitment of the HAT PCAF and the HMT Set1/Ash2, for H3K9 acetylation and H3K4 trimethylation, respectively. We propose that at class II promoters NM1 activates transcription through a chromatin-based mechanism that coordinates recruitment of chromatin remodelers and preserves active epigenetic marks.
Measuring NM1 occupancy across the mammalian genome
It is therefore plausible that at the promoter NM1 regulates Pol II transcription and may do so through a chromatin-based mechanism that leads to correct deposition of the epigenetic marks H3K4me3, H3k9ac and H3k27ac.
Transcription activation at class II promoters requires NM1
We next asked whether NM1 performs a potential regulatory function by facilitating Pol II association with the gene promoter. For this, we applied ChIP to crosslinked chromatin from MEFs subjected to NM1 gene silencing, using antibodies against unphosphorylated and hypophosphorylated Pol II. We also used antibodies against actin and the actin-binding core subunits Rpb6 (RPABC2) and Rpb8 (RPABC3) common to all nuclear RNA polymerases [32, 33] which are also known to directly interact with the largest Pol II subunit. The results from the qPCR analyses with primers amplifying the promoters of the mouse Rad9a and Rpl19 genes (selected from the NM1 ChIP Seq analysis among the NM1 top binders) revealed drops in the levels of hypophosphorylated Pol II, actin, Rpb6 and Rpb8 as a consequence of NM1 gene silencing whereas the levels of unphosphorylated Pol II did not significantly change (Fig. 4 d-g).
Since phosphorylation of the Pol II CTD occurs during transcription activation and marks Pol II engagement in the transcriptional process, the above results altogether suggest that NM1 may specifically activate transcription by maintaining Pol II in complex with actin at the gene promoter.
NM1 regulates chromatin changes compatible with Pol II transcription activation
These results suggest that at those gene promoters where NM1 regulates association of both SNF2h and WSTF, NM1 may have an impact on the local structure of chromatin. We therefore began to look for NM1-dependent changes in chromatin accessibility by applying a high resolution micrococcal nuclease (MNase) assay [25, 34] on chromatin isolated from NM1 silenced MEFs. The results from the qPCR analyses with primers amplifying regions upstream and downstream the transcription start site (−250 kb to +100 kb) of the Rpl19 gene promoter show that knocking down the NM1 gene induced only marginal chromatin protection over the Rpl19 gene promoter at position −70 (Fig. 5d), as revealed by somewhat decreased MNase accessibility. However, the extent of chromatin protection was significantly enhanced when we performed MNase digestions on chromatin isolated from HEK293T cells stably expressing mutated NM1 constructs that function as dominant negatives in transcription [20, 25] (see also Fig. 5h). Specifically, we used HEK293T cells expressing wild-type V5-tagged NM1 (V5-wt NM1), a mutated NM1 variant that cannot bind to actin (V5-RK605AA NM1) but avidly interacts with SNF2h  while displaying decreased chromatin binding ability (Additional file 7: Figure S5) or a deletion construct lacking the C-terminus (V5-ΔC NM1) which cannot interact with the chromatin (Fig. 5e; Additional file 7: Figure S5; see also ref. ). The results from the qPCR analyses with primers amplifying regions upstream and downstream the transcription start site (−350 kb to +100 kb) of the human RPL19 gene promoter show that expression of either V5-wtNM1 or V5-ΔC NM1 did not generally affect chromatin accessibility (Fig. 5f). In contrast, stable expression of V5-RK605AA NM1 produced a significant closing of the chromatin at position −70 kb similar to the NM1 knockdown situation but considerably enhanced (Fig. 5f). The same experiment also revealed the significant establishment of a hypersensitive site further upstream at position −140 (Fig. 5f). We next performed ChIP on chromatin isolated from HEK293T cells expressing V5-wtNM1 or V5-RK605AA NM1 with antibodies to actin, WSTF and SNF2h as well as a control antibody. The results from the qPCR analysis with primers amplifying the same region around position −70 within the human RPL19 gene promoter show that expression of RK605AA NM1 induced a drop in the levels of actin, SNF2h and WSTF (Fig. 5g). These results demonstrate the requirement for a fully functional NM1 for actin association with the promoter (see also Fig. 4d and e). Moreover, these results suggest that the decreased levels of SNF2h upon stable RK605AA NM1 expression are due to SNF2h sequestration that negatively regulates chromatin, since the RK605AA NM1 mutant interacts with SNF2h and displays decreased chromatin binding efficiency. If NM1 is important for Pol II transcription activation and affects occupancies of actin and SNF2h, Pol II transcription levels should drop in the cells that stably express NM1 mutants that cannot interact with actin or with chromatin. Indeed, RT-qPCR analysis of the relative RAD9A and RPL19 mRNA levels on total polyA mRNA isolated from HEK293T cells stably expressing V5-RK605AA NM1 and V5-ΔC NM1 shows significant drops in Pol II transcription in comparison to wild-type (Fig. 5h; Additional file 7: Figure S5). Further, ChIP and qPCR analysis on chromatin isolated from HEK293T cells expressing V5-wtNM1, V5-RK605AA NM1 or V5-ΔC NM1 with antibodies to actin, hypophosphorylated Pol II CTD as well as control antibodies to non-specific IgGs show that expression of RK605AA NM1 and V5-ΔC NM1 induced drops in the levels of actin and Pol II at the promoters of the RAD9A, RPL19 genes (Fig. 5i). These findings confirm that NM1 plays a primary role in Pol II transcription and further support that NM1 activates transcription by maintaining Pol II in complex with actin at the gene promoter.
We conclude that a functional NM1 is required to regulate recruitment of the chromatin remodeler SNF2h and histone modifiers in order to ensure an active chromatin state which is compatible with Pol II transcription.
We describe, for the first time, the functional association of a myosin motor, NM1, with a mammalian genome. ChIP-Seq analysis demonstrates that NM1 binds to both non-coding and coding regions of the mouse genome. When partitioning the coding sequences into promoter, exons, introns and UTRs, we found that NM1 is particularly enriched at class II promoters, compatible with a role for NM1 in the initial phases of Pol II transcription.
Our present findings also underscore the importance of the interaction between NM1 and SNF2h to start transcription at class II promoters. Genome-wide analysis by ChIP-Seq shows that NM1 and SNF2h co-localize at multiple genomic locations. The overlap between NM1 and SNF2h is not complete, consistent with a more general role of the chromatin remodeler SNF2h in the context of other nuclear functions . Interestingly, however, NM1 silencing generally impaired SNF2h binding to the chromatin at class II promoters but led to gene-specific fluctuations in the levels of WSTF. These findings suggest that WSTF forms a complex with NM1 and SNF2h only at a subset of promoters. At these promoters (such as the mouse Rpl19 and Rad9a promoters), consistent with the finding that WSTF is required to co-precipitate NM1 and SNF2h from nuclear fractions , the WSTF-bound chromatin is likely to mark the precise location for B-WICH assembly. NM1, on the other hand, seems to play an important role in stabilizing B-WICH to exert its function. We found that stable expression of the transcriptional dominant negative RK605AA NM1 mutant led to a local closing of chromatin and a drop in the occupancy levels of SNF2h. Considering the avid interaction between the RK605AA NM1 mutant and SNF2h , during WSTF-dependent B-WICH assembly, NM1 possibly stabilizes the association of SNF2h with WSTF and thus with the chromatin, and this is a pre-condition for SNF2h to catalyze correct repositioning of nucleosomes (Fig. 7b). It is tempting to hypothesize that this, in turn, produces a major impact on Pol II transcription by controlling histone H1 dynamics . We therefore propose that following B-WICH assembly, SNF2h-mediated remodeling opens up the chromatin to make it accessible to those histone modifications that occur immediately after repositioning of the nucleosomes and appear to be dependent on NM1. Indeed, NM1 gene knockdown led to decreased levels of epigenetic marks for active transcription, including H3K9ac, H3K4me3 and H3K27ac, but did not affect the levels of H3K4me1. These changes correlate with NM1-dependent drops of PCAF and Set1/Ash2. Therefore, it is likely that both histone modifiers are physically recruited by NM1to the gene promoter, conceivably when NM1 does not interact with actin . In conclusion, we propose that the active epigenetic marks H3K9ac and H3K4me3 are under the direct control of NM1 but require a local pre-setting of the chromatin mediated by SNF2h in order to be fully executed and promote transcription activation (Fig. 7c).
Class 1 myosins serve as divalent crosslinkers, physically connecting and generating force between actin filaments and cargos such as membrane lipids . Our results suggest that in the cell nucleus NM1 does not seem to be an exception to the rule, although unconventional modes of action are possible. In our working model, the role of NM1 at class II promoters is dictated by its motor activity. It is linked to a cycle of attachment and detachment from dynamic Pol II-associated actin and provides a physical link between Pol II and chromatin. We speculate that the polymerase-associated actin undergoes dynamic changes in the polymerization state yet to be understood. The model translates into a temporal framework that coordinates the functions of actin in positioning hypophosphorylated Pol II at a specific site within the gene promoter, with the role of SNF2h as chromatin remodeler followed by H3K9ac, H3K4me3 and H3K27ac. This mechanism ultimately ensures that the Pol II machinery is exposed to a local chromatin landscape compatible with transcription activation as it lowers the nucleosome barrier and allows for efficient promoter clearance . NM1 is enriched at the gene promoter but it also distributes across the gene. We therefore speculate that the above mechanism may also be important for Pol II transcription elongation and NM1 may synergize not only with SNF2h but with a cohort of chromatin remodelers.
Transcriptional activation is a fundamental cellular process essential for living organisms and it is spread across the entire genome. The association of NM1 with a large number of class II genes therefore argues that NM1 is a general factor involved in transcription activation. We speculate that this positive role in conjunction with actin and with the WSTF/SNF2h complex regulates genes that must be rapidly reprogrammed through chromatin de-repression in order to be activated. Nuclear actin and components of the SNF2 members of chromatin remodelers are important in transcriptional reprogramming [39–42]. A recent study has specifically shown the importance of SNF2h to establish gene expression programs underlying cerebellar morphogenesis and neural maturation in mice . We therefore hypothesize that as a consequence of its motor function NM1 balls between actin and SNF2h to regulate open chromatin states, providing a genome-wide mechanism that rapidly establishes and preserves transcriptional programs during decisions important for cell fate and behavior.
Cell culture and reagents
Mouse embryonic fibroblasts (MEFs), wild-type HEK293T cells and HEK293T cells constitutively expressing the V5-tagged NM1 constructs V5-wtNM1, V5-RK605AA NM1 or V5-ΔC NM1 were grown in DMEM medium (Gibco, Life Technology, Carlsbad, CA, USA), supplemented with 10 % fetal bovine serum (Gibco) and a 1 % penicillin/streptomycin cocktail (Gibco) as previously performed [25, 26]. The antibodies against WSTF (ab50850), SNF2h (ab3749), H3K4m1 (ab8895), H3k4me3 (ab8580), H3K9Ac (ab10812) and H3k27ac (ab4729), Set1/Ash2 (ab70378), Rpb8 (ab104802), as well as 8WG16 (ab817) and 4H8 (ab5408) respectively targeting non-phosphorylated and hypophosphorylated (phospho-S5) heptapeptide repeats from the CTD of the largest Pol II subunit were all purchased from Abcam, Cambridge, UK. The antibodies against PCAF (sc13124) and Rpb6 (sc28711) were from Santa Cruz Biotechnology, Inc. Dallas, TX, USA. The antibodies to actin are specific for the β-isoform and were purchased from Sigma-Aldrich, St. Louis, MO, USA (clone AC74). The antibody against the V5 epitope (A190-120A) was purchased from Bethyl, Montgomery, TX, USA Laboratories. The non-specific rabbit IgGs (ab46540) were from Abcam. The antibody against NM1 has previously been characterized . The anti-pan-myosin-Ic monoclonal antibody that recognizes an epitope in the tail region of myosin Ic has been previously characterized  and was provided by Dr. W. Hofmann (University at Buffalo-SUNY). RNAi duplexes against the target sequence GCACACGGCUUGGCACAGA in the mouse NM1 (NM1 RNAi) or control scrambled versions (scrRNAi) were purchased from Dharmacon, Lafayette, CO, USA; GE Healthcare and applied by transfection with Lipofectamine RNAi Max (Invitrogen, Waltham, Massachusetts, USA) at a final concentration of 30 nM. RNAi duplexes against human NM1 or control scrambled versions were previously described and they were applied by transfection with Lipofectamine RNAi Max (Invitrogen) at a final concentration of 30 nM as previously described . The HEK293T cells stably expressing V5-wt NM1, V5-RK605AA NM1 and V5-ΔC NM1 were a kind gift of Ingrid Grummt, German Cancer Research Center, Heidelberg, Germany .
MEFs transfected with NM1 RNAi duplexes or control scrRNAi duplexes were grown in 10 cm dishes for 24 h at 37 °C. Total RNA was extracted with the TRI reagent as specified by the manufacturer’s instruction manual (Sigma). For analysis of transcripts, polyA mRNA was isolated from NM1-silenced or control cells using the Oligotex mRNA Mini Kit according to the manufacturer’s protocol (Qiagen, Venlo, Limburg, Netherlands) and treated with DNase1. cDNA was then synthesized using Superscript II reverse transcriptase (Invitrogen) using oligo dT primers according to the manufacturer’s instructions. The concentration of cDNA was determined by nanodrop. Semi-quantitative RT-qPCR was performed using the cDNA templates prepared from MEFs treated with control scrRNAi duplexes or from MEFs treated with NM1 RNAi duplexes, a Power SYBR Green PCR kit (Life Technology, Carlsbad, CA, USA), specific primers amplifying the mouse class II genes Rplp0, Rpl13a, Rpl19, Junb, Rad9a, Wtap, Ddx46 as well as Bad and the human class II genes RAD9A and RPL19 (see Additional file 8: Table S3) and a 7300 Real Time PCR System (Applied Biosystems, Waltham, Massachusetts, USA). For all primers the annealing temperature was 60 °C. All samples were run in triplicate. Relative changes in RNA levels were calculated against the reference β-actin gene using the delta-delta Ct method as previously described .
Cells were lysed in RIPA buffer (50 mM Tris‐HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 % NP‐40, 0.5 % sodium deoxycholate, 0.1 % SDS) supplemented with protease inhibitors (cOmplete cocktail, Roche, Basel, Switzerland). For denaturation, protein extracts were incubated in Laemmli buffer at 95 °C for 10 min, separated by SDS–PAGE under reducing conditions and transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, Massachusetts, USA) by semidry blotting (Biorad, Hercules, CA, USA). Primary antibodies and dilutions used were NM1 (1:1000), β-actin (Sigma, 1:50000), WSTF (Abcam, 1:2000), SNF2h (Abcam, 1:500), PCAF (Abcam, 1:500), Set1/Ash2 (Abcam, 1:500). Immunoreactive bands were visualized by chemiluminescence (Amersham, GE Healthcare Life Sciences, Pittsburgh, USA).
High-resolution MNase assay
These experiments were essentially performed as described [25, 34]. Briefly, HEK293T cells subjected to control or NM1 gene knockdown by RNAi , and HEK293T stably expressing V5-wtNM1, V5-RK605AA NM1 or V5-ΔC NM1 were crosslinked with 1 % formaldehyde for 20 min. Chromatin was prepared as for ChIP (see below), but washed with Buffer D containing 25 % glycerol, 5 mM magnesium acetate, 50 mM Tris (pH 8.0), 0.1 mM EDTA, 5 mM DTT. Before digestion with MNase the chromatin was lightly sonicated in MNase buffer (60 mM KCl, 15 mM NaCl, 15 mM Tris at pH 7.4, 0.5 mM DTT, 0.25 M sucrose, 1.0 mM CaCl2), 8 times for 30 s. The equivalent of 0.46 × 106 cells was used in each reaction, and the level of DNA was first adjusted to be in the same range in the samples from all different treatments. Several MNase concentrations were used such that the reaction occurred in the linear range of digestion. Two samples from each treatment were used for the calculations: 10 U MNase and one concentration between 10 U and 20 U MNase. The reactions were performed at 37 °C for 30 min and then stopped by adding 12.5 mM EDTA/0.5 % SDS. After three hours of proteinase K treatment, the cross-linking was reversed at 65 °C for five hours. DNA was extracted  and the digest was evaluated by qPCR with primers amplifying around the transcription start site of the mouse Rpl19 gene (primers available upon request) and human RPL19 gene (for the mouse primers sequences see Additional file 9: Table S4), giving a product of approximately 100 bp. The results were analyzed by calculating ΔCt between the reactions performed with and without MNase. The values are presented as 2ΔCt. Chromatin from cells transfected with control siRNA oligonucleotides and chromatin from untransfected cells gave the same MNase digestion pattern. P-values (significances) were obtained by Student’s t-test as previously described .
ChIP and qPCR analysis
ChIP on growing MEFs was performed as previously described . Briefly, formaldehyde cross-linked chromatin was obtained from in vivo cross-linked MEFs and subjected to immunoprecipitations with antibodies to Pol II (8WG16 and 4H8), Rbp6, Rbp8, NM1, actin, WSTF, SNF2h, H3K9Ac, H3K27Ac, H3K4me1, H3K4me3, PCAF, Set1/Ash2, HDAC1 and non-specific rabbit IgGs. DNA-protein complexes were analyzed by qPCR with specific primers amplifying class II promoters (see Additional file 10: Table S5 for the primers sequences). qPCR was performed using SYBR-green from Applied Biosystems according to the manufacturer’s instructions. The primer concentration was 2.5 mM and the samples analyzed by qPCR (7300 Real Time PCR System, Applied Biosystem). The PCR conditions were: hold 50 °C for 2 min, 95 °C for 10 min, 95 °C for 15 s, 60 °C for 1 min, 95 °C for 15 s. The results were analyzed using an average of Ct of no antibody as background. The 2ΔCt of each sample in triplicate was related to the 2ΔCt of the input sample. P-values (significances) were obtained by Student’s t-test as previously described .
ChIP assays were also performed on formaldehyde crosslinked chromatin isolated from wild-type HEK293T and HEK293T cells expressing V5-wtNM1, V5-RK605AA NM1 and V5-ΔC NM1 mutants using antibodies against the V5 epitope, NM1 and histone H3 as well as non-specific rabbit IgGs. Precipitated chromatin was analyzed by PCR with primers to the EP300 gene promoter and exonic sequences and the PCR products visualized by agarose gel electrophoresis. For the primers sequences see .
ChIP-Seq, sequencing, data alignment and analysis
For ChIP-Seq analysis, crosslinked chromatin from MEFs was subjected to immunoprecipitation with antibodies to NM1. A total of 5 ng of precipitated DNA was used to prepare sequencing libraries at the Bejing Genome Institute (Hong Kong) using the Illumina HiSeq 2000 platform. The analysis procedure involved the use of the SOAP2 program to map the reads to the mouse reference genome. Sequences with more than two mismatches were discarded from further analysis. The resulting individual sequences were remapped back to the annotated UCSC MM9 reference sequence which allows for the identification of peaks corresponding to the levels of association of the ChIP target with those loci. The ChIP-Seq data sets are available for download in the Gene Expression Omnibus (GEO) database (accession number GSE66542). The ChIPseek program was used to analyze the genomic distribution of NM1 across coding and non-coding elements and around the transcription start site (TSS) . For this all genomic locations with a score of 10 sequences per 50 bp or above were selected. The ChIPseek program was also used for pairwise comparison of the NM1 ChIPSeq data with the SNF2h ChIPSeq data .
We are grateful to Wilma Hofmann (University at Buffalo-SUNY) for providing us with the anti-pan-myosin 1C antibody. We also thank Ghasem Nurani, Martin Corcoran, Magnus Hansson and Raffaele Mori for technical help. This work was supported by grants from the Swedish Research Council (Vetenskapsrådet) and the Swedish Cancer Society (Cancerfonden) to PP. BA is co-funded by NGHA-KAIMRC, Saudi Arabia. AAS was co-funded by a Karolinska Institute doctoral fellowship (KID).
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