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The Toll pathway underlies host sexual dimorphism in resistance to both Gram-negative and Gram-positive bacteria in mated Drosophila

  • David F. Duneau1, 2Email author,
  • Hannah C. Kondolf1, 3,
  • Joo Hyun Im1, 4,
  • Gerardo A. Ortiz1,
  • Christopher Chow1,
  • Michael A. Fox1,
  • Ana T. Eugénio5,
  • J. Revah1, 4,
  • Nicolas Buchon1, 4 and
  • Brian P. Lazzaro1, 4
Contributed equally
BMC Biology201715:124

https://doi.org/10.1186/s12915-017-0466-3

Received: 13 October 2017

Accepted: 30 November 2017

Published: 21 December 2017

Abstract

Background

Host sexual dimorphism is being increasingly recognized to generate strong differences in the outcome of infectious disease, but the mechanisms underlying immunological differences between males and females remain poorly characterized. Here, we used Drosophila melanogaster to assess and dissect sexual dimorphism in the innate response to systemic bacterial infection.

Results

We demonstrated sexual dimorphism in susceptibility to infection by a broad spectrum of Gram-positive and Gram-negative bacteria. We found that both virgin and mated females are more susceptible than mated males to most, but not all, infections. We investigated in more detail the lower resistance of females to infection with Providencia rettgeri, a Gram-negative bacterium that naturally infects D. melanogaster. We found that females have a higher number of phagocytes than males and that ablation of hemocytes does not eliminate the dimorphism in resistance to P. rettgeri, so the observed dimorphism does not stem from differences in the cellular response. The Imd pathway is critical for the production of antimicrobial peptides in response to Gram-negative bacteria, but mutants for Imd signaling continued to exhibit dimorphism even though both sexes showed strongly reduced resistance. Instead, we found that the Toll pathway is responsible for the dimorphism in resistance. The Toll pathway is dimorphic in genome-wide constitutive gene expression and in induced response to infection. Toll signaling is dimorphic in both constitutive signaling and in induced activation in response to P. rettgeri infection. The dimorphism in pathway activation can be specifically attributed to Persephone-mediated immune stimulation, by which the Toll pathway is triggered in response to pathogen-derived virulence factors. We additionally found that, in absence of Toll signaling, males become more susceptible than females to the Gram-positive Enterococcus faecalis. This reversal in susceptibility between male and female Toll pathway mutants compared to wildtype hosts highlights the key role of the Toll pathway in D. melanogaster sexual dimorphism in resistance to infection.

Conclusion

Altogether, our data demonstrate that Toll pathway activity differs between male and female D. melanogaster in response to bacterial infection, thus identifying innate immune signaling as a determinant of sexual immune dimorphism.

Keywords

Sexual dimorphismInnate immunityAntimicrobial peptides Drosophila melanogaster Toll pathway

Background

The most striking differences among individuals in a population are often those between the sexes. Such dimorphism is often characterized by obvious differences in morphology and behavior, as well as in a number of differences in physiological functions, including immunity, metabolism, and disease outcome [1]. The sex of the host influences both the dynamics of infections [2] and host symptoms [3], with a possible impact on parasite adaptation to the host when transmission is sex biased [4]. Despite the plethora of examples of sexual dimorphism in disease outcomes [3, 5, 6], the characterization of these differences have been largely overlooked in medical studies and in studies of natural systems [711]. Herein, we used Drosophila melanogaster to understand the basis of sexual dimorphism of an innate immune system.

D. melanogaster is a powerful model system with a well-defined innate immune response [12]. The cellular response to bacteria consists of defensive phagocytosis by specialized cells called plasmatocytes (functional equivalent of mammalian macrophages). The humoral response in adults is characterized by oxidative melanization and production of antimicrobial peptides (AMPs). The production of AMPs is regulated by two pathways, the Imd and the Toll pathways, which are homologous to the vertebrate TNF and Toll-like pathways, respectively [12]. The Imd pathway is activated upon the detection of peptidoglycan produced by Gram-negative bacteria, whereas the Toll pathway responds to the peptidoglycan of most Gram-positive bacteria and to proteases secreted during pathogenic infections [13]. Activation of each of these pathways leads to the nuclear translocation of transcription factors in the NF-κB family, driving a robust transcriptional response to infection [14, 15]. D. melanogaster exhibit sexual dimorphism in disease outcome. Recent studies have reported that males and females differ in response to gut [16] and systemic bacterial infection [17, 18], as well as to infection by pathogenic fungi [19, 20] and viruses [21]. However, the molecular basis behind this dimorphism remains unknown.

Here, we establish that infection with a broad spectrum of Gram-positive and Gram-negative bacteria results in a sexually dimorphic outcome in both outbred and inbred populations of mated D. melanogaster. This is not due only to the previously described [2224] increased susceptibility of mated females. We dissect in detail the genetic and mechanistic basis for the dimorphic response to infection with Providencia rettgeri, a Gram-negative bacterial pathogen that naturally infects wild D. melanogaster. We find that the Toll signaling pathway is sexually dimorphic in genome-wide constitutive gene expression and in induced response to infection. Higher expression of Toll signaling genes and higher activity of Persephone-mediated Toll signaling explains the higher resistance of males relative to females.

Methods

Host husbandry and genotypes

D. melanogaster were reared until adulthood on glucose-yeast medium (100 g/L yeast, 100 g/L glucose, 1% agar). At day 2 after eclosion, adults were isolated in groups of five males and five females per vial. All experiments were conducted with mated individuals 5 to 8 days post-eclosion unless stated otherwise. Husbandry and experiments were conducted at 24 °C (±1 °C) with a 12 h:12 h light:dark cycle. We used different D. melanogaster genotypes – Canton-S (Bloomington stock # 1), Oregon R (Bloomington stock # 5), and w1118 (Bloomington stock #6326) as ‘wildtype’ laboratory strains and an outbred population that was derived from the Global Diversity Lines [25, 26] and kindly provided by J. Ayroles and A. Clark. To label phagocytes, we used the reporter lines Hemolectin-Gal4 > UAS-GFP, eater-nls::GFP, Peroxidasin-Gal4 > UAS-GFP, and eater-Gal4 > UAS-dsred [2729]. To test the contribution of different components of innate immunity to sexual dimorphism in survival to infection, we used a series of null mutants, namely (1) PPO1 Bc fj 1 wt 1 [30] and triple mutant PPO1Δ,2Δ,31 [31, 32], which have deficient melanization, (2) PGRP- LE 112 ;+;PGRP-LC ΔE [33], imd 10191 [34], dTAK1 D10 [35], and Relish E20 [36], which have deficient Imd pathway function, and (3) spz rm7/TM6C [37], modSP α33 [38], and psh 1 [39], which have deficient Toll pathway function. To test the contribution of cellular immunity, we used phagocyte-depleted flies generated by inducing the pro-apoptotic gene Bax in Hml-expressing hemocytes (Hml-Gal4 > UAS-GFP, UAS-bax, Gal80ts) [40, 41]. We avoided potential deleterious developmental effects of phagocyte depletion by rearing the flies at 18 °C through pupation, and then switching freshly eclosed adults to 29 °C for 3 days, driving expression of the bax toxin. Phagocyte-depleted flies were then returned to 25 °C for 2 days before injection. Since there is no hematopoietic organ in D. melanogaster adults, these flies remained without phagocytes even after return to 25 °C (Additional file 1: Figure S4A).

Bacterial strains and infection

We used a variety of bacterial strains, including both Gram-negative and Gram-positive bacteria known to vary in virulence in D. melanogaster. For Gram-negative bacteria, we used Pectinobacterium carotovora carotovora 15 (formerly known as Erwinia carotovora carotovora 15), Ecc15-GFP [42], and Escherichia coli (pEGFP, Mach1-T1 strain) with low virulence (i.e., does not kill at low dose), as well as strains of two Providencia species isolated from wild-caught D. melanogaster [43, 44], namely P. rettgeri (strain Dmel and its GFP-transformed derivative used in visualization experiments), which is moderately virulent (i.e., kills 80–50% of the flies infected at low dose), and P. alcalifaciens (strain Dmel), which is highly pathogenic (i.e., all flies infected at low dose die within a day). For Gram-positive bacteria, we used Enterococcus faecalis, a moderately virulent bacterium isolated from wild-caught D. melanogaster [45] and Staphylococcus aureus (PIG1), a highly virulent bacterium. Liquid cultures of each bacterium were grown to saturation overnight at 37 °C, except Ecc15, which was grown at 29 °C. Saturation cultures were pelleted, then resuspended and diluted in phosphate buffered saline (PBS, pH 7.4) to an optical density (OD) of 0.1 (600 nm wavelength) unless stated otherwise. We injected 23 nL of bacterial suspension into each fly abdomen using a Nanoject II (Drummond), corresponding to a dose of approximately 3000 viable bacteria per fly (for OD = 0.1). Injection of the same volume of sterile PBS was used as a wounding treatment. Flies injected with PBS experienced minimal to no mortality during the experiments. Flies were anesthetized with CO2 for less than 5 min during the injection procedure, and then were observed shortly after injection to confirm recovery from manipulations. The number of individuals and of experimental repetitions are indicated by ‘df +1’ for the factor ‘Experiment’ in each analysis reported in the figure legends.

Host survival

We monitored host survival after injection in groups of 50 males and 50 females kept together in 900-mL plastic boxes with ad libitum access to food, monitoring survival daily for 10 days unless otherwise specified. We focused our study on the first 10 days of the infection to avoid an interaction between the effect of ‘sex’ and ‘age’ in response to infection. Individuals alive after 10 days were recorded as censored observations. In experiments where sexual dimorphism was compared between genetic lines, injected individuals were kept solitary in vials to avoid potential confounding effects of density due to differential rates of mortality among the groups. Host survival differences were analyzed using a Cox’s proportional hazards mixed model, with ‘Sex’ and ‘Dose’ as main effects and ‘Replicate’ as random effect using the R package ‘Survival’ [46]. The cox.zph function from the same package was used to ensure that the data fit the assumptions of the proportional hazards approach.

Bacterial load

To monitor bacterial loads, flies were individually homogenized in 500 μL of sterile PBS with a HT homogenizer (OPS Diagnostics). The homogenate was then diluted 1:10 in PBS and 50 μL of this suspension was plated onto LB agar using a WASP II autoplate spiral plater (Microbiology International). Plates were incubated overnight at 37 °C (endogenous microbiota do not appear on LB plates during this time) and bacterial colonies were counted using a EZ-Count Automated Colony Counter (Microbiology International) to estimate the number of viable bacteria per fly. Bacterial load differences were compared with a Kruskal–Wallis or a Wilcoxon test when possible. Because non-parametric tests can be underpowered, we additionally performed a parametric Welsh t test. When ‘Time post-injection’ and ‘Line’ were included in the analysis, we used a linear model assuming a Gaussian distribution of the error. The residual error was checked for normality and homoscedasticity, and when necessary the raw data were subjected to Box–Cox transformation. To include ‘Replicate’ as a random effect, we used a generalized linear mixed model with the function HLfit from the R package ‘spaMM’ [47]. In that case, P values were obtained from model selections and likelihood ratios (LR2) were given.

Hemolymph quantification

To estimate the quantity of hemolymph in male and female hosts, we weighed 3- to 5-day-old flies individually on a Mettler Toledo (MX5) microbalance to the nearest microgram, before and after removing the hemolymph. We removed the hemolymph by separating the thorax and abdomen of individual flies and absorbing the hemolymph by contact with a Kimwipe (Kimberly Clark) as previously described [48].

Phagocyte counts and phagocytosis

In order to quantify hemocytes, Hml-Gal4 > UAS-GFP, eater-nlsGFP, and Pxn-Gal4 > UAS-GFP flies were decapitated and injected with sterile PBS (Nanoject II; Drummond) to detach sessile phagocytes from the tissues. Groups of 5 or 15 same-sex flies were then bled onto a glass slide, which was imaged on a Zeiss microscope (axioplot imager Zeiss) [49]. For each slide, the number of phagocytes per 25 mm2 was counted using Zeiss’ Zen software. We analyzed the difference in phagocyte numbers between males and females with paired Wilcoxon tests, pairing males and females of the same genotype and manipulating them in parallel. To estimate the proportion of phagocytes actively engaged in phagocytosis, we injected flies with 23 nL of 1 mg/mL pHrodo-labeled dead E. coli (Molecular Probes, cat# P35361), which becomes fluorescent upon phagosome maturation, and counted the number of phagocytes containing labeled bacteria using the same procedure. To confirm that P. rettgeri was phagocytized by phagocytes, we injected bacteria carrying a plasmid expressing GFP into host flies expressing dsRed specifically in phagocytes (eater-Gal4 > UAS-dsred). We recorded the phagocytosis events with confocal microscopy (axioplot imager Zeiss).

Estimation of the time before control of bacterial proliferation (\( {\overline{t}}^c \))

We estimated the averaged time required for the host to control bacterial proliferation (\( {\overline{t}}^c \)) as performed previously [50]. In short, we built a model that consists of a mixture of three models, one describing within-host bacterial growth, one modeling bacterial reduction if the host controls the infection, and one modeling the likelihood that a given fly controls or fails to control the infection. These models were then fit to empirical data of bacterial growth trajectories in males and females, using eight individually plated flies per hour post-injection to estimate t c for each sex. Confidence intervals for parameter estimates were obtained by bootstrapping the empirical data for each time point and re-estimating model parameters.

RNA isolation and library construction

We measured the whole transcriptional response in male and female Canton S 8 h after the infection started. Flies were injected with approximately 3000 bacteria in suspension in 23 nL of PBS as described above. A subset of the flies was dissected to remove their reproductive tract prior to RNA isolation. Each sample was a same-sex pool of 25 flies homogenized in 1 mL of TRIzol (Life Technologies). Unchallenged flies were simultaneously treated the same way as injected flies, except that they were only exposed to CO2 and did not receive an injection. We quantified expression in biological triplicates of unchallenged and infected pools. Six additional pools of 25 unchallenged flies for each sex were added to estimate the constitutive expression of the Toll pathway. Those additional pools were exposed to CO2 at the same time as the initial pools but were homogenized 24 and 72 h after being anesthetized. RNA and library quality was determined by AATI Fragment Analyzer. Libraries were prepared using the Lexogen Quantseq 3’ mRNA-Seq Library Prep kit, following manufacturer’s instructions. Sequencing was performed on the Illumina Hiseq 2500 Rapid Run Mode platform at the Cornell Life Sciences Sequencing Core, with 50 bp single-end reads.

Read mapping, normalization and quantification of expression differences

For each sample, at least 5 million sequence reads were generated. Read quality was estimated by fastqc for quality control and reads were trimmed using Trimmomatic ([51], version 0.32). Trimmed reads were then mapped to the D. melanogaster reference genome (version 6.80), using STAR RNA-seq aligner ([52], version 2.4.1a). Counts were calculated using ‘htseq’ ([53], version 0.6.1). We characterized the difference between males and females in transcriptional response to infection by the interaction between the treatment (unchallenged vs. infected) and the sex (male vs. female). The model was then Number of reads = Treatment + Sex + Treatment*Sex. We performed the analysis with the R package ‘DEseq2’ [54] and visualized the significant candidates (i.e., P value lower than 0.05 after an FDR correction) in volcano plots using the R package ggplot2 [55]. Heatmap figures were generated with the package ‘Heatmap3’ with default settings to calculate the clustering [56]. Gene ontology (GO) enrichment analysis was performed with the software Gorilla (available at http://cbl-gorilla.cs.technion.ac.il/) to search for GO categories that are enriched in the ‘target’ set (herein, the list of female genes differently expressed in infected host compared to non-infected) compared to the ‘background’ set (herein, the list of male genes differently expressed in infected host compared to non-infected) using the standard Hyper Geometric statistics [57].

Quantification of host gene expression by RT-qPCR

To evaluate Toll pathway activity, we used qPCR to measure the expression of Drosomycin, an antimicrobial peptide gene strictly regulated by the Toll pathway [13], using primers CGTGAGAACCTTTTCCAATATGATG (forward) and TCCCAGGACCACCAGCAT (reverse). To evaluate Imd pathway activity, we measured the expression of Diptericin, an antimicrobial peptide gene strictly regulated by the Imd pathway [13] using primers GCGGCGATGGTTTTGG (forward) and CGCTGGTCCACACCTTCTG (reverse). Inter-sample variation in cDNA content was controlled by analysis of reference gene RpL32 (Forward primer: GACGCTTCAAGGGACAGTATCTG, Reverse primer: AAACGCGGTTCTGCATGAG), which has been shown to not respond to infection [58]. We obtained total RNA from groups of eight to ten flies at 24 and 72 h after injection of P. rettgeri or PBS. In a second experiment, we obtained the total RNA from groups of four flies at 8 h after injection of P. rettgeri (before any death happened) or unchallenged control individuals. Flies were homogenized in 1 mL of TRIzol (Life Technologies), immediately stored at –80 °C, and RNA was isolated using a standard protocol. One microgram of DNase-treated (Promega) RNA was reverse-transcribed using MLV-RT (Promega). The cDNA thus produced served as template for quantitative PCR using the SSO Advanced SYBR Green Kit (Bio-Rad) under minor modification of the manufacturer’s instructions.

Results

Sexual dimorphism in host susceptibility to infection is pervasive

In order to test whether D. melanogaster are sexually dimorphic in survivorship in response to infection, we first injected a genetically diverse population as well as several inbred populations of D. melanogaster with both Gram-negative (P. rettgeri and P. alcalifaciens) and Gram-positive (E. faecalis and S. aureus) bacteria that vary in virulence. In all cases, we found significant differences in survival between males and females from this outbred population (Fig. 1a–d). Females were generally more likely than males to die from infection and from an injection wound (Fig. 1e), irrespective of wildtype genotype (Fig. 1f–h and Additional file 2: Figure S1A) and initial bacterial dose (Fig. 1i and Additional file 2: Figure S1B, C). Interestingly, however, the sex dimorphism in host susceptibility was reversed after infection with S. aureus (Fig. 1d). This indicates that females are not globally weaker but rather that they are specifically more susceptible to most bacterial pathogens. We considered that the females in our study might be immunocompromised, as mating and reproduction have previously been shown to suppress immunity in D. melanogaster females [2224]. To test this hypothesis, we compared the survival of mated and virgin females to mated males after infection with P. rettgeri. The mortality of virgin females was intermediate between those of mated males and of mated females (Fig. 1j). This result suggests that female mating status contributes to the sexual dimorphism in survival but is not sufficient to completely account for the effect. To further characterize host sexual dimorphism in susceptibility, we focused on a natural pathogen of Drosophila that shows moderate levels of virulence, the Gram-negative bacterium P. rettgeri.
Fig. 1

Sexual dimorphism in survival to infection of an outbred population. a Survival upon infection with the Gram-negative bacteria P. rettgeri. Females were more susceptible than males (Cox-ph: df = 1, χ2 = 23.26, P < 0.0001). b Survival upon infection with the Gram-positive bacteria Enterococcus faecalis. Females were more susceptible than males (Cox-ph: df = 1, χ2 = 5.96, P = 0.015). c Survival upon infection with the Gram-negative bacteria P. alcalifaciens. Females were more susceptible than males (Cox-ph: df = 1, χ2 = 42.49, P < 0.0001). d Survival upon infection with the Gram-positive bacteria Staphylococcus aureus. Males were more susceptible than females (Cox-ph: df = 1, χ2 = 7.37, P = 0.006). e Survival upon injection of PBS (sterile solution used for bacterial suspension). Females were slightly more susceptible than males (Cox-ph: df = 1, χ2 = 5.66, P = 0.02). f Survival of the genotype Canton S upon infection by the Gram-negative bacteria P. rettgeri. Females were more susceptible than males (Cox-ph: Sex: df = 1, χ2 = 49.42, P < 0.0001). g Survival of the genotype Oregon R upon infection by the Gram-negative bacteria P. rettgeri. Females were more susceptible than males (Cox-ph: Sex: df = 1, χ2 = 16.09, P < 0.0001). h Survival of the genotype w 1118 upon infection by the Gram-negative bacteria P. rettgeri. Females were more susceptible than males (Cox-ph: Sex: df = 1, χ2 = 4.36, P = 0.036). i Dose response in survival of Canton S to P. rettgeri infection. Females were more susceptible than males (Cox-ph: Sex: df = 1, χ2 = 96.97, P < 0.0001) and the dose had an effect on survival (Dose: df = 1, χ2 = 8.09, P = 0.004). This effect was dependent on the host sex as a 100-fold difference in starting dose was not enough to remove the dimorphism in survival (Dose*Sex: df = 1, χ2 = 5.64, P = 0.017). j Survival of mated males and females and virgin females upon infection with the Gram-negative bacteria P. rettgeri. Mated males are more resistant than mated females (Cox-ph adjusted for multiple tests: Sex: df = 1, z = 4.6, P < 0.001) and virgin females (Cox-ph adjusted for multiple tests: Sex: df = 1, z = 2.34, P = 0.045). Mated and virgin females were marginally non-significant in difference in survival (Cox-ph adjusted for multiple tests: Sex: df = 1, z = 2.31, P = 0.054)

Females are less efficient at controlling pathogen growth

To elucidate whether males and females differ in their ability to control pathogen proliferation, we infected males and females with P. rettgeri and quantified the bacterial load in each individual (Fig. 2). We first monitored the sexual dimorphism in bacterial burden in a wildtype (Canton S) host at different time points of the infection (Fig. 2a) and determined that the burden seems to stabilize between 2 and 3 days post-injection, at the same time when host mortality plateaued (Fig. 1f). Surviving flies sustain persistent infection and do not eliminate the bacteria. While we did not detect a sexual dimorphism in resistance before the chronic persistence phase (8, 16, and 24 h), females carried more than 10 times the bacterial load during the chronic phase of infection (3, 5, 7, and 10 days; Fig. 2a). This difference in bacterial load during the chronic phase of the infection was also observed in our outbred population at 3, 10, and 30 days post-injection (Fig. 2b) and in other inbred wildtype genotypes at 10 days post-injection (Fig. 2c). In order to make sure that the difference in bacterial burden was not due to the sexual dimorphism in body size, we weighed the total mass and the hemolymph of males and females from three wildtype genotypes (Additional file 3: Figure S2). Females were only approximately 20% larger than males and had about twice the quantity of hemolymph as males, whereas the difference in bacterial load was 10-fold. Thus, bacterial load does not scale as a simple function of fly size. We have recently shown that flies die at a fixed bacterial burden, called the Bacterial Load Upon Death (BLUD) [50], which is the maximum number of bacteria a fly can sustain before dying. We quantified the BLUD for males and females by checking infected hosts every 30 min and determining the bacterial load in newly dead flies at each observation point. We did not detect sexual dimorphism in BLUD of either Canton S or Oregon R genotypes (Fig. 2d), suggesting that males and females die at a similar bacterial burden.
Fig. 2

Sexual dimorphism in resistance to P. rettgeri. a P. rettgeri load in Canton S (CS) flies over 10 days of infection. Overall, females carried more bacteria than males. We did not detect differences in bacterial load in early time points (Wilcoxon test: 8 h: W = 151.5, P = 0.1102; 16 h: W = 151, P = 0.1148; 24 h: W = 60, P = 0.52). However, after 48 h post-injection, when the mortality plateaued and the infection can be considered chronically persistent, females carried a higher burden than males (Wilcoxon test: 2 days: W = 99.5, P = 0.01; 3 days: W = 171.5, P = 0.0007; 5 days: W = 202.5, P = 0.0002; 7 days: W = 188, P = 0.0003; 10 days: W = 152, P = 0.0005). b P. rettgeri load upon injection in flies from an outbred population. Females carried more bacteria than males during the chronic phase of infection of P. rettgeri in the three independent experiments looking at three different time points of the infection (Wilcoxon test: 3 days: W = 262.5, P = 0.01; 10 days: W = 1162, P < 0.0001; 30 days: W = 2148, P < 0.0001). c P. rettgeri load 10 days post-injection in flies from wildtype genotypes. For the three genotypes, females carried more bacteria than males during the chronic phase of infection of P. rettgeri (Wilcoxon test: CS: W = 350, P < 0.0001; w1118: W = 692.5, P = 0.0001; Oregon R (OrR): W = 513, P = 0.0001). d Bacterial load upon death (BLUD; +30 min maximum) in both sexes of CS and OrR flies. We did not detect a significant sexual dimorphism in P. rettgeri load at death (Welsh t test: CS: df = 255.28, t = –1.77, P = 0.08; OrR: df = 235.58, t = 0.6, P = 0.54). Both sexes succumbed at the same bacterial load. Black lines in b, c, and d represent the means. The violin plots represent the distribution of the data, the quartiles and the median. Wilcoxon tests comparing medians: *P < 0.05, ****P < 0.0001, and ns: P > 0.05. e Within-host dynamic of P. rettgeri in males and female CS flies between 8 and 16 h post-injection. In both experiment replicates (i and ii), males have controlled the bacterial proliferation before females (estimated t c , or time of control, is represented by the vertical red lines and the confidence interval around the estimate is shaded in gray). Each dot represents the bacterial load in a single fly, the solid line represents the standard Baranyi bacterial population growth fitted to the white dots (see Methods and [50]). The intensity of red in the dots represents the inferred probability that the host controlled the infection (see Methods and [50])

We then focused on the initial phase of infection to better understand the correlation between bacterial proliferation within the host and sexual dimorphism in survival. We previously showed that the survival outcome of infection with P. rettgeri for each individual depends of the time at which the immune response becomes effective and can control bacterial proliferation (time to control) [50]. Using our previously published mixture model and a bootstrap approach on hourly bacterial load data from the 8th to the 16th h after injection (see Methods), we estimated the average time to control \( {\overline{t}}^c \) in males and females (Fig. 2e). Individual male flies were more likely to control the infection (illustrated as red dots in Fig. 2e) as a population, and males started to control infection earlier than females did (Fig. 2e). We found that the \( {\overline{t}}^c \) differed by 2–3 h between males and females, with little or no overlap between the confidence intervals on \( {\overline{t}}^c \) estimation, corresponding to a difference of roughly 5–8 bacterial doublings [43] prior to control.

Our data suggested that the sexual dimorphism in susceptibility to infection was due to sexual dimorphism in within-host bacterial growth. We considered that differences in bacterial growth might be due to a difference in resources available to bacteria infecting males and females. To investigate this, we injected P. rettgeri into living hosts or into hosts that were killed by decapitation either at the moment of the injection or 1 h before the injection. Since dead hosts are unable to mount an inducible immune response, we reasoned that sexual dimorphism in bacterial growth would be maintained in dead hosts if it was due to differences in resources available to the pathogen within male and female hosts, but lost if the dimorphic response was due to inducible responses. We did not see any difference in bacterial growth within dead male and female hosts, despite the sexual dimorphism in living hosts (Additional file 4: Figure S3). We therefore concluded that that the sexual dimorphism was due to a difference in active control of the proliferation, such as by the innate immune response.

Cellular immunity and melanization are not responsible for sexual dimorphism in response to P. rettgeri

Hemocytes perform defensive phagocytosis and promote resistance to pathogens [40, 59, 60]. We thus asked whether sexual differences in phagocyte counts and in phagocytosis efficiency could underlie the sexually dimorphic response to P. rettgeri. We first confirmed that P. rettgeri can be phagocytized by injecting GFP-labeled P. rettgeri into flies expressing dsRed in their phagocytes (eater-dsRed) and observing co-localization of green and red fluorescence under confocal microscopy (Additional file 1: Figure S4B). We observed that females of three different D. melanogaster genotypes that express GFP specifically in their phagocytes had more phagocytes than males (Additional file 1: Figure S4C, left panel), which is the opposite of what would be expected if differential phagocytosis was responsible for the dimorphism. We then tested whether males and females differed in phagocytosis efficiency, using dead pHrodo-labeled E. coli that fluoresce red only when in the low-pH phagolysosome compartment. We found no difference in the number of phagocytes with bacteria in their phagolysosome compartment (Additional file 1: Figure S4C, right panel), indicating no difference in the capability for phagocytosis. Finally, we directly tested the requirement for phagocytes in host survival of P. rettgeri infection by measuring survival (Fig. 3Ai) and bacterial load (Fig. 3Aii) of wildtype flies compared to flies whose hemocytes had been genetically ablated at the adult stage. We saw little effect of phagocyte ablation on survivorship of infection or on systemic pathogen load in either sex and the dimorphism persisted even in the absence of phagocytes (Fig. 3A). These collective results suggest that phagocytosis is not the key immune response that generates immune sexual dimorphism.
Fig. 3

Phagocytosis, melanization, and the Imd pathway do not explain the sexual dimorphism. a The sexual dimorphism in survival to P. rettgeri is still present when hosts are not able to phagocytose bacteria. i Survival of flies lacking phagocytes compared to their genetic control after infection by P. rettgeri (OD = 0.1). Females of both lines were more susceptible than males and the lack of phagocytes did not affect the probability of surviving the infection (Cox-ph: Line: df = 1, χ2 = 1.01, P = 0.32; Sex: df = 1, χ2 = 18.41, P = 0.02; Line*Sex: df = 1, χ2 = 0.45, P = 0.50). ii P. rettgeri growth in flies lacking phagocytes and in their genetic control. Even if P. rettgeri was phagocytized, the bacterial growth was not significantly affected by this immune response neither in early time points (Linear regression: Line: df = 1, F = 0.28, P = 0.59; Time: df = 1, F = 577.82, P < 0.0001; Sex: df = 1, F = 3.63, P = 0.05; Time*Sex: df = 1, F = 0.0003, P = 0.99; Line*Sex: df = 1, F = 0.039, P = 0.84), nor at 10 days post-injection (Linear regression: Line: df = 1, F = 0.62, P = 0.43; Sex: df = 1, F = 20.36, P < 0.0001; Line*Sex: df = 1, F = 1.07, P = 0.3). b The sexual dimorphism in survival to P. rettgeri is still present when hosts are not able to melanize bacteria. i Black cell loss-of-function mutant (Cox-ph: Sex: df = 1, χ2 = 5.15, P = 0.02). ii Triple mutant PPO1Δ, 2Δ,31 loss-of-function (Cox-ph: Sex: df = 1, χ2 = 4.46, P = 0.03). c The sexual dimorphism in survival to P. rettgeri is still present when hosts are not able to induce an Imd response against bacteria. Females were more susceptible to P. rettgeri than males for the four mutants tested. i Imd loss-of-function mutant (Cox-ph: Sex: df = 1, χ2 = 156.24, P < 0.0001). ii Relish loss-of-function mutant (Cox-ph: Sex: df = 1, χ2 = 230.99, P < 0.0001). iii Double loss-of-function mutant PGRP-LC, PGRP-LE (Cox-ph: Sex: df = 1, χ2 = 71.9, P < 0.0001). iv P. rettgeri growth in Relish loss-of-function mutant. The infection started with the same number of bacteria in both sexes (Linear regression: Sex: df = 1, F = 1.02, P = 0.32). The bacterial populations have grown over time (Time: df = 1, F = 766.96, P < 0.0001) but the growth was significantly different between sexes (Time*Sex: df = 1, F = 5.32, P = 0.02). Imd response does not explain the sexual dimorphism in susceptibility to P. rettgeri

We next tested the role of immune-regulated melanization [31] as potentially contributing to dimorphism in susceptibility to P. rettgeri infection. We compared survival between males and females in the melanization-deficient mutant ‘Black cells’ and in a fly deficient for all three prophenoloxidases (key enzymes for melanization). In both cases, females deficient for melanization were more susceptible to infection than deficient males (Fig. 3B), suggesting that melanization does not play a role in the dimorphism. Altogether, our results show that the sexual dimorphism in resistance to P. rettgeri infection is not due to differences in cellular immunity or immune melanization between males and females.

Imd pathway is central to controlling P. rettgeri growth but is not responsible for the dimorphism in susceptibility

Gram-negative bacteria induce the Imd pathway, which is critical for surviving these infections [13]. Furthermore, Imd pathway-regulated genes have been shown to have higher expression in D. melanogaster males than in females [61]. We therefore tested whether the Imd pathway is responsible for the observed sexual dimorphism in susceptibility to P. rettgeri infection by infecting several mutants that are deficient in Imd pathway activity with P. rettgeri and monitoring their survival. We used loss-of-function mutants of multiple genes involved in the Imd pathway, namely Imd (Imd 10191 ) [34], Relish (Rel E20 ) [36], dTAK1 (dTAK1 D10 ) [35], and a double mutant for both PGRP-LC and PGRP-LE (PGRP- LE 112 ;+;PGRP-LC ΔE ) [33]. Separately testing this complete set of mutants has the effect of querying the pathway at multiple points and of assessing the role of the Imd pathway in multiple genetic backgrounds. All of the mutant strains were much more susceptible to P. rettgeri infection than wildtype strains, with 100% of the mutant individuals dying in less than 48 h, thus confirming that the Imd pathway is absolutely required to fight P. rettgeri infection. Nevertheless, for all the Imd mutants tested, female flies still died significantly more quickly than males and the dimorphism was preserved (Fig. 3C and Additional file 5: Figure S5A). Interestingly, the exponential growth of P. rettgeri in Imd-deficient hosts over 16 h was also faster in females than in males (Fig. 3Civ) and sexual dimorphism was present in bacterial load at 8 and 12 h post-injection in Relish mutants (Additional file 5: Figure S5B). This suggests that, in absence of the Imd pathway, the hosts still retain some ability to control the infection and that ability is sexually dimorphic. Thus, we conclude that, while the Imd pathway is important for defense against P. rettgeri infection, it does not underlie the observed sexual dimorphism.

Difference in Toll pathway induction causes sexual dimorphism in susceptibility

The Toll pathway is canonically thought to underlie resistance to Gram-positive bacteria and fungi [12, 13], but can also be activated upon infection with pathogenic Gram-negative bacteria [62] and upon wounding [6366]. To test whether the Toll pathway contributed to sexual dimorphism in immunity, we infected null mutants of spaetzle (spz) [37], which encodes the ligand for Toll in Drosophila, and monitored survival of both males and females. Null mutants of spz exhibited a mild susceptibility to P. rettgeri infection, but more importantly, they exhibited no sexual dimorphism in survivorship (Fig. 4a). This result indicates that a functional Toll pathway is required for the dimorphic susceptibility to P. rettgeri infection.
Fig. 4

The Toll pathway is responsible for the sexual dimorphism in survival. a Sexual dimorphism in susceptibility in a loss-of-function mutant for the Toll pathway (spaetzle mutants). The probability of dying from P. rettgeri infection was the same for female and male hosts (Cox-ph: Sex: df = 1, χ2 = 1.12, P = 0.29). b Sexual dimorphism in susceptibility in loss-of-function mutant for the ModSP branch of the Toll pathway. Females were more susceptible than males (Cox-ph: Sex: df = 1, χ2 = 7.66, P = 0.005). Recognition of P. rettgeri by the ModSP component of the Toll pathway is not the reason for the sexual dimorphism in susceptibility. c Sexual dimorphism in susceptibility in loss-of-function mutant for the Persephone branch of the Toll pathway. The probability of dying from P. rettgeri infection was not different between female and male hosts (Cox-ph: Sex: df = 1, χ2 = 0.11, P = 0.74). The recognition of the virulence factor of the pathogenic Gram-negative bacteria P. rettgeri by the Toll pathway allows the sexual dimorphism. d Sexual dimorphism in susceptibility in loss-of-function mutant for the ModSP branch and for the Persephone branch of the Toll pathway. Females were less susceptible to E. faecalis infection than males for both mutant lines; males, but not the females, mutant for the Persephone branch where likely to die earlier than males mutant for the ModSP branch (Cox-ph: Line: df = 2, χ2 = 3.71, P = 0.05; Sex: df = 1, χ2 = 72.45, P < 0.0001; Line/Sex: df = 2, χ2 = 7.46, P = 0.006). Recognition of host damage or virulence factors is likely to be more important than the direct recognition of pathogenic bacteria. e Relative expression of Drosomycin to RpL32 in both sexes in response to the infection by P. rettgeri. Toll response (i.e., difference in Drosomycin expression between unchallenged and infected) was stronger 8 h post-injection in male wildtypes and mutants for modSP (Interaction Sex x Treatment: Canton S: df = 1, F = 13.68, P = 0.002; modSP: df = 1, F = 17.18, P = 0.0048) but this dimorphism was not present in mutants of Persephone and spaetzle (psh: df = 1, F = 0.35, P = 0.46; spz: df = 1, F = 0.03, P = 0.86)

A critical step in the activation of the Toll pathway is the cleavage of Spz, which allows it to bind to and activate the Toll receptor. This cleavage can be stimulated by either of two mechanisms [38, 39]. First, recognition of stereotypical cell wall components of fungi and Gram-positive bacteria leads to the proteolytic activation of a Serine protease, ModSP, that initiates a cascade of proteolytic events ultimately leading to Spz processing (the ‘ModSP branch’, see illustration in Fig. 5C) [38]. Alternatively, the Toll pathway can be activated by recognition of virulence factors or host tissue damage through the cleavage of a distinct Serine protease, Persephone, which also leads to the processing of spz (the ‘Psh branch’, see illustration in Fig. 5C) [39, 67, 68]. We asked which branch of the pathway is responsible for Toll-mediated sexual dimorphism by disrupting each branch independently. In modSP mutants, females still continue to die significantly more rapidly from P. rettgeri infection than males do, although the dimorphism is marginally attenuated (Fig. 4b). In contrast, however, loss-of-function of the persephone gene completely abolished the sexual dimorphism in survivorship of P. rettgeri infection (Fig. 4c). We confirmed those outcomes using RNAi targeting ModSP and Persephone transcripts (Additional file 6: Figure S6A). Thus, sensing of P. rettgeri infection by Persephone allows the recognition of the infection by the Toll pathway and its dimorphic activity ultimately leads to a difference in susceptibility to this infection between males and females.
Fig. 5

Whole transcriptome response to infection suggest a major role for the constitutive expression of the Toll pathway in the sexual dimorphism. a Transcriptional difference in response to infection in whole body of males and females. b Transcriptional difference in response to infection in carcasses (i.e., reproductive tissues were removed before RNA collection) of males and females. Those volcano plots represent the genes according to the dimorphism of the response (i.e., interaction Sex and Treatment in our model) and the significance of the difference. Blue dots are genes from which the response (i.e., difference between unchallenged and infected) is significantly more upregulated in males (or downregulated in females). Red dots are genes from which the response is significantly more upregulated in females (or downregulated in males). Grey dots are non-significant genes after FDR correction for multiple testing. c Illustration of the Toll pathway and sexual dimorphism of its constitutive expression based on a publically available meta-analysis [72]. Numbers in brackets represent the male/female ratio of gene expression in whole flies. Males constitutively express genes in the part of the pathway that is specific to the immune function at higher levels than females (part of the pathway outside the host cell, ‘Imm. only’) and females express genes that are used in embryonic development more highly (part of the pathway inside the host cell, ‘Imm. & Dev.’). d Constitutive expression of genes belonging to the Toll pathway in nine biological replicates of a pool of 25 non-infected male or female flies. The male-specific constitutive expression of the genes involved only in immunity (black) and the female-specific constitutive expression of the genes shared between immune functions and embryonic development (grey) were confirmed in samples including gene expression in reproductive tissues (i), but disappeared when those tissues were removed by dissection prior to transcriptional analysis (ii). The gradient in the heatmaps represents the level of expression (blue: low and red: high)

The Toll pathway is central for the immune response against Gram-positive bacteria [12, 13]. In order to test whether Toll activity is generally dimorphic, we tested whether the Toll pathway also causes dimorphism in response to infection with the Gram-positive bacterium E. faecalis. Like P. rettgeri, this natural pathogen of D. melanogaster induced greater mortality in female than male wildtype hosts (Fig. 1b and Additional file 2: Figure S1C). Female and male mutants for the Toll pathway were equivalently susceptible to E. faecalis infection, again indicating that the Toll pathway is required for the immune dimorphism. Furthermore, when we injected male and female flies mutant for either modSP or persephone with E. faecalis, we observed a surprising reversal in the direction of the sexual dimorphism compared to infection in wildtype flies. Males died slightly but significantly earlier than females (Fig. 4d), with males null for Persephone dying earlier than males null for modSP (Fig. 4d). This finding indicates that in response to E. faecalis infection, both the ModSP and Persephone branches have a role in the sexually dimorphic outcome.

Our data suggest that Toll pathway activity or activation could differ between males and females, which we could expect to cause difference in the production of AMPs. We therefore evaluated the expression levels of an antimicrobial peptide gene controlled by Toll (Drosomycin) [13]. The induction of Drosomycin is much stronger in males than in females at both 24 and 72 h post-injection (Additional file 6: Figure S6B), indicating sustained and dimorphic activation of the Toll pathway in response to the Gram-negative P. rettgeri infection. This sexually dimorphic activation was lost in Psh and Spz mutants but not in ModSP mutants (Fig. 4e). The Spz mutation eliminated the dimorphism in AMP expression levels more completely than the Psh mutation. Our results suggest that the Psh/Toll branch controls the dimorphic expression of the antimicrobial peptide genes.

The role of Psh in the dimorphism in susceptibility reveals that sensing of damage or bacterial virulence is key in the immune dimorphism of D. melanogaster. To test whether the observed sexual dimorphism in immunity is conditional on a pathogenic bacterial infection, we evaluated the capacity of Canton S males and females to manage infection with the non-lethal bacteria E. coli and Ecc15 (survival > 95%). These Gram-negative bacteria should activate the Imd pathway but are not predicted to activate the Toll pathway through either the ModSP (peptidoglycan sensing) or Persephone (damage sensing) branches. In accordance with that hypothesis, we saw no difference between males and females in the sustained load of either Ecc15 or E. coli over a 3-day time course (Additional file 7: Figure S7). These data indirectly support a model where the Persephone/Toll immune cascade that responds to virulence factors is central to Drosophila innate immune dimorphism.

The transcriptional response to infection is sexually dimorphic

Having demonstrated that the Toll pathway is primarily responsible for the sexual dimorphism in response to infection, we next undertook a transcriptomic analysis to identify whether other processes might make secondary contributions to dimorphism. We used RNA-seq to quantify changes in gene expression of males and females at 8 h post-injection, with data taken either from the entire fly or from dissected carcasses in which the reproductive tract and gonads were removed. The resulting data are available and can be browsed on our website (http://flysexsick.buchonlab.com/) or in supplementary Additional file 8: Tables S1 and Additional file 9: Table S2. When expression was quantified from the whole body, we detected four genes for which the response was significantly different between male and female hosts after FDR correction, measured by interaction between Sex and Treatment in the statistical analysis. These genes are Drosomycin, Defensin, CG4267, and takeout (Fig. 5A and Additional file 10: Figure S8A). Drosomycin and Defensin encode antimicrobial peptides and are downstream of the Toll pathway [13]. The observation that these genes were more highly expressed in males than in females confirms the role of the Toll pathway in establishing dimorphism. CG4267, involved in the lipid catabolic process, was also more strongly induced in males. Takeout, involved in nutritional homeostasis [69], was downregulated upon infection in males but was not differentially regulated in females.

Because our transcriptional measures were taken from the whole body, apparent differences in the transcriptional response could be due to or masked by differences in gene expression in reproductive tissue between males and females. We therefore performed RNA-seq on the carcasses of males and females from which the reproductive tracts had been dissected away. We again found that Drosomycin expression responded more strongly in males than in females (Fig. 5B, Additional file 10: Figure S8B). We additionally confirmed that the male-specific response of takeout was independent of the presence of the reproductive tract. In contrast, the dimorphic response of Defensin disappeared in the absence of reproductive tissue, indicating that defensin is regulated dimorphically in the reproductive tract with stronger expression in males. The analysis of the dimorphic response of carcasses also revealed additional genes with a dimorphic response to infection that were not identified in whole flies. Transferrin 1 was more strongly upregulated in females, which suggests a dimorphic role for iron sequestration in the response to infection. Three stress-responsive genes regulated by the Jak-Stat pathway, TurandotM, TurandotA, and TurandotC were strongly induced by infection in males. Even in the absence of reproductive tissue, however, there were few genes with markedly sexually dimorphic regulation (i.e., from which the interaction between Treatment and Sex was significant). We undertook a GO enrichment analysis where we compared the enrichment of GO categories that are differentially regulated after infection of females with those that are differentially regulated after infection of males. The only GO categories that were then differently enriched between males and females were categories involved in antibacterial humoral response (Tables 1 and 2). This was true regardless of whether the reproductive tract had been removed and supports the interpretation that differences in Toll pathway activity are primarily responsible for the dimorphism in resistance.
Table 1

Gene ontology enrichment analysis of gene expression in response to P. rettgeri in entire flies

GO term

Description

P valuea

FDR q valueb

Enrichment (N, B, n, b)c

GO:0006952

defense response

4.09 × 10–8

4.71 × 10–5

2.70 (181, 38, 37, 21)

GO:0009617

response to bacterium

2.35 × 10–7

1.36 × 10–4

2.73 (181, 34, 37, 19)

GO:0043207

response to external biotic stimulus

1.18 × 10–6

4.52 × 10–4

2.45 (181, 40, 37, 20)

GO:0009607

response to biotic stimulus

1.18 × 10–6

3.39 × 10–4

2.45 (181, 40, 37, 20)

GO:0051707

response to other organisms

1.18 × 10–6

2.71 × 10–4

2.45 (181, 40, 37, 20)

GO:0051704

multi-organism process

2.03 × 10–6

3.91 × 10–4

2.39 (181, 41, 37, 20)

GO:0006955

immune response

2.29 × 10–6

3.77 × 10–4

2.68 (181, 31, 37, 17)

GO:0009605

response to external stimulus

5.7 × 10–6

8.22 × 10–4

2.28 (181, 43, 37, 20)

GO:0002376

immune system process

7.72 × 10–6

9.9 × 10–4

2.52 (181, 33, 37, 17)

GO:0006950

response to stress

1.56 × 10–5

1.8 × 10–3

2.10 (181, 49, 37, 21)

GO:0042742

defense response to bacterium

3.43 × 10–5

3.6 × 10–3

2.53 (181, 29, 37, 15)

GO:0050896

response to stimulus

5.73 × 10–5

5.51 × 10–3

1.81 (181, 65, 37, 24)

GO:0098542

defense response to other organisms

5.94 × 10–5

5.28 × 10–3

2.45 (181, 30, 37, 15)

GO:0050829

defense response to Gram-negative bacterium

2.8 × 10–4

2.31 × 10–2

2.88 (181, 17, 37, 10)

GO:0002682

regulation of immune system process

3.07 × 10–4

2.36 × 10–2

4.19 (181, 7, 37, 6)

GO:0050776

regulation of immune response

3.07 × 10–4

2.22 × 10–2

4.19 (181, 7, 37, 6)

GO:0031347

regulation of defense response

6.73 × 10–4

4.57 × 10–2

3.42 (181, 10, 37, 7)

GO:0019731

antibacterial humoral response

9.88 × 10–4

6.33 × 10–2

2.75 (181, 16, 37, 9)

aThe enrichment P value computed according to the mHG or HG model. This P value is not corrected for multiple testing of 1636 GO terms

bThe correction of the above P value for multiple testing using the Benjamini and Hochberg method

cDefined as follows: N is the total number of genes, B is the total number of genes associated with a specific GO term, n is the number of genes in the top of the user’s input list or in the target set when appropriate, b is the number of genes in the intersection; enrichment = (b/n)/(B/N)

Table 2

Go enrichment table of gene expression in response to P. rettgeri in dissected flies

GO term

Description

P valuea

FDR q valueb

Enrichment (N, B, n, b)c

GO:0006955

immune response

4.77 × 10–5

7.8 × 10–2

1.57 (331, 35, 181, 30)

GO:0050896

response to stimulus

6.02 × 10–5

4.92 × 10–2

1.31 (331, 95, 181, 68)

GO:0006959

humoral immune response

1.62 × 10–4

8.81 × 10–2

1.62 (331, 26, 181, 23)

GO:0002376

immune system process

2.44 × 10–4

9.99 × 10–2

1.49 (331, 38, 181, 31)

GO:0006950

response to stress

3.5 × 10–4

1.15 × 10–1

1.31 (331, 78, 181, 56)

GO:0009617

response to bacterium

3.69 × 10–4

1.01 × 10–1

1.45 (331, 43, 181, 34)

GO:0009605

response to external stimulus

4.03 × 10–4

9.41 × 10–2

1.39 (331, 54, 181, 41)

GO:0043207

response to external biotic stimulus

4.49 × 10–4

9.18 × 10–2

1.40 (331, 51, 181, 39)

GO:0009607

response to biotic stimulus

4.49 × 10–4

8.16 × 10–2

1.40 (331, 51, 181, 39)

GO:0051707

response to other organisms

4.49 × 10–4

7.34 × 10–2

1.40 (331, 51, 181, 39)

GO:0019731

antibacterial humoral response

7.21 × 10–4

1.07 × 10–1

1.71 (331, 16, 181, 15)

aThe enrichment P value computed according to the mHG or HG model; this P value is not corrected for multiple testing of 1636 GO terms

bThe correction of the above P value for multiple testing using the Benjamini and Hochberg method

cDefined as follows: N is the total number of genes, B is the total number of genes associated with a specific GO term, n is the number of genes in the top of the user’s input list or in the target set when appropriate, b is the number of genes in the intersection; enrichment = (b/n)/(B/N)

Sexual dimorphism in resistance to infection could originate from constitutive differences between the sexes prior to infection. We therefore examined the transcriptomic differences between males and females in the absence of infection. We particularly focused on genes related to the Toll pathway. Our hypothesis was that sexual dimorphism in activation of the Toll pathway could be explained by sexually dimorphic constitutive expression of genes encoding components of the Toll signaling cascade, thereby generating dimorphism in immune response. The intracellular signaling component of the Toll pathway is additionally required for dorsoventral patterning of the Drosophila embryo in the female ovary [70]. Pattern recognition receptors (e.g., PGRP-SA, GNBP1, GNBP3), as well as serine proteases of the Toll pathway (e.g., Persephone, ModSP, Grass, SPE) are required for the activation of Spz during an immune response (illustrated outside the host cell in Fig. 5C and referred as ‘Imm. only’ in Fig. 5D), whereas Spz is activated by an independent process leading to the serine protease Easter during development [71]. Genes coding for activation of Toll and downstream intracellular signaling participate in both immune and developmental signaling (including Spaetzle, Myd88, tube, Pelle, Cactus, illustrated in the host cell in Fig. 5C and referred as ‘Imm. & Dev.’ in Fig. 5D). To evaluate the possibility that genes encoding Toll pathway components are expressed constitutively in a dimorphic manner, we undertook two approaches. First, we retrieved the expression levels of these key genes from published transcriptomic datasets obtained from either males or females [72]. These data revealed that genes coding for components of the Toll signaling pathway were constitutively expressed in a sexually dimorphic fashion. Genes from the Toll pathway involved in the immune activation of AMPs were expressed mostly in a male-biased fashion (Ratio M/F expression: Spe: 1.8, grass: 1.5, modSP: 1.5, GNBP3: 1.25, PGRP-SA: 1.46, GNBP1: 1, persephone: 1, Fig. 5C). However, downstream genes in the pathway, involved both in immune and germline Toll pathway activity, were significantly female biased (Ratio M/F expression: spaetzle: 0.5, Myd88: 0.76, tube: 0.43, pelle: 0.43, Fig. 5C). Second, we evaluated our own expression data from the unchallenged condition (Fig. 5D). We found that sex and involvement in the immune response were primary determinants of hierarchical gene expression clustering (‘Imm. only’ genes vs. ‘Imm. Dev.’ genes in males vs. females Fig. 5Di), confirming the results observed in the meta-analysis. This pattern was, however, not present when the expression in the reproductive tract was removed from the samples (Fig. 5Dii). This suggests strongly that the difference in developmental RNAs are due to the maternal depositions into eggs and are not part of the systemic immune response. Altogether, these results support the hypothesis that the Toll pathway plays a central role in the sexual dimorphism of the response to P. rettgeri infection. They also suggest that constitutive sexually dimorphic expression of genes regulating Toll pathway activity is a key determinant of sexual dimorphism in resistance to infection.

Discussion

Across taxa, studies have shown that disease outcomes are often sexually dimorphic (reviewed in [73]). However, the mechanisms underlying such dimorphism remain poorly characterized. In this study, we show that D. melanogaster females are generally less resistant to bacterial infections due to decreased relative activity of the Toll signaling pathway and therefore have a reduced capability of mounting an effective immune response. Drawing from the model proposed by Duneau et al. [50], this reduction in immune inducibility could lead to decreased probability that females control bacterial proliferation in the early stages of infection, thus increasing the probability that they ultimately die from the infection. Although the Toll pathway is generally considered to control Gram-positive bacterial and fungal infections, we found it to be responsible for observed dimorphism in susceptibility to both the Gram-positive bacterium E. faecalis and the Gram-negative P. rettgeri. We note that this dimorphism in Toll activity effectively results in male and female hosts being different ‘environments’ from the perspective of the parasite, with potential consequence for the evolution of pathogens that have sex-biased transmission.

What makes males and females dimorphic

The activation of the Toll pathway is triggered downstream of either the recognition of bacterial cell wall components (ModSP branch) or the detection of proteolytic activity in hemolymph (Persephone branch) (Fig. 5C). Persephone has been proposed to also mediate Toll activation downstream of endogenous danger signals [39]. The requirement of the Persephone branch for the increased male survivorship of P. rettgeri infection suggest two hypotheses. On the one hand, it is possible that the dimorphic response is directly due to a dimorphism in recognition of danger signals or virulence factors through Persephone-mediated sensing. On the other, it is also possible that the Persephone branch is not itself dimorphic, but that its activation by pathogenic infection exposes inherent dimorphism in downstream components of the Toll pathway; the latter hypothesis seems more likely. The genes of the Toll pathway that are specific to the immune system are constitutively male-biased in their expression (Fig. 5C, D), supporting the idea that once the recognition occurs via the Persephone branch, the Toll pathway can respond more strongly in males, leading to a higher expression of the AMPs. Furthermore, it is also possible that, in addition to this dimorphism in constitutive expression, a dimorphism in activation of the pathway during the infection may translate into subsequent differences between sexes.

Proximal cause for the immune sexual dimorphism

Although the Toll pathway is clearly dimorphic between males and females, the basis for this dimorphism remains to be determined. One reasonable hypothesis is that hormonal differences between females and males generate the difference in Toll activity. The importance of juvenile hormone (JH) in oogenesis and in female response to mating supports the hypothesis that males and females may have different levels of circulating JH. JH has been shown to downregulate the immune system with a stronger influence on the Toll pathway than on the Imd pathway (as shown by Figure 1 of Flatt et al. [74]). Mating and the transfer of male seminal fluid proteins affects the female innate system [75], stimulating the production of JH in female D. melanogaster [76, 77] and depressing immune performance in mated females relative to virgins [23, 24]. Since we found dimorphism even between virgin females and mated males, it is possible that the dimorphism in the expression of the Toll pathway is the result of dimorphic levels of JH in females even before mating. The immunosuppressant effect of hormones is frequently proposed to be the main proximal mechanism for sexual dimorphism in vertebrate susceptibility to infection [78], although studies on mammals have emphasized the acquired immune system with little attention to the innate immune system. Based on the Drosophila data, we propose that endocrinologic differences may also influence sexual dimorphism in innate immunity.

Evolutionary consequences of immune sexual dimorphism

From the perspective of an infecting pathogen, male and female hosts may be different ‘environments’ with, among other variations, different levels of immune activity. It is thus reasonable to assume that parasite lineages could adapt to these distinct environments either when transmission is sex biased, either due to unequal host sex-ratio, sex-based social structure, or endogenous susceptibility of one host sex relative to the other [70]. Furthermore, dimorphism in host resistance, as is the case against P. rettgeri infections, imposes a specific kind of selection pressure on pathogens. Theory predicts that hosts with a strong immune response select for more virulent parasites with a higher transmission. In the case of extreme difference in resistance, evolution of increased virulence in one sex may result in a parasite that kills the non-resistant sex so quickly that the pathogen can persist and evolve only in the resistant host [79]. In the case of sexual dimorphism in host resistance, as observed in our study, sex-specific adaptation of parasites is therefore expected to evolve [4, 73]. Our results show that D. melanogaster can have a strong sexual dimorphism in resistance to certain bacterial infections and can therefore be a good model to explore the consequences of sex-specific adaptation on sex-specific symptoms and prevalence of infectious diseases.

From the host perspective, females are expected to invest more in immunity in order to maximize lifetime reproduction (Bateman’s principle of immunity) or because of the direct negative interaction between reproductive and immune traits (immunocompetence handicap hypothesis) [80, 81]. These simplified evolutionary models have often led to the prediction that animals should have an immunologically ‘weaker sex’ [5], and tend to ignore the variability that has already been highlighted. Indeed, males from mammalian species are generally more susceptible to infectious diseases like leishmaniasis, malaria, and schistosomiasis and females to toxoplasmosis, amoebiasis, and giardiasis [6, 78, 82]. In D. melanogaster, the observation that males were more susceptible than females to S. aureus infections highlights the parasite dependence of sexual dimorphism in immunity. The sex-specific investment in defense is probably the result of independent investments in different functions of the immune system (e.g., humoral and cellular responses) linked to sex-specific constraints or pleiotropy, and not of a general weakness [61]. Indeed, in Drosophila, S. aureus infection is generally controlled by phagocytosis and melanization and the recognition of S. aureus infection by the Toll pathway can be limited [31, 83, 84]. Which sex has higher relative susceptibility may therefore depend on the identity of the infecting parasite, exposing difficulties in drawing generalizations about the sexual dimorphism via meta-analyses [85]. As we begin to consider personalized medicine and bridging the ‘gender-gap’ in biomedical studies as well as the role of host sex in epidemiology, it appears increasingly important to consider, in specific details, the host dimorphism in response to infection.

Declarations

Acknowledgements

We thank Patrícia Beldade for comments on the manuscript. We thank Madeline Galac for providing the P. rettgeri GFP strain, Andrew Clark and Julien Ayroles for providing the D. melanogaster outbred population, and Jean-Baptiste Ferdy for statistical advice.

Funding

This work was funded by NIH grant R01 AI083932 to BPL, NSF 1354421 and funding from the Empire State Stem Cell Fund through New York State Department of Health NYSTEM contract C029542 to NB, and a fellowship from the Swiss National Foundation (P300P3_147874) and from the French Laboratory of Excellence project ‘TULIP’ (ANR-10-LABX-41; ANR-11-IDEX-0002-02) to DFD. The LIA BEEG-B (Laboratoire International Associé-Bioinformatics, Ecology, Evolution, Genomics and Behaviour) (CNRS) provided support for travel.

Availability of data and materials

The data from the RNA-seq are available and can be browsed on our website http://flysexsick.buchonlab.com/, or in Additional file 8: Tables S1 and Additional file 9: Table S2.

Authors’ contributions

DFD, BPL, and NB designed the experiments and wrote the manuscript. DFD analyzed the data. DFD, GAO, HCK, CC, MAF, and NB performed the bacterial experiments. DFD, JHI, and ATE performed the molecular work. JR did the initial datamining of the transcriptome and developed the website. All authors read and approved the manuscript.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Université Toulouse 3 Paul Sabatier, CNRS, ENFA, UMR5174 EDB (Laboratoire Évolution & Diversité Biologique), Toulouse, France
(2)
CNRS, Université Paul Sabatier, UMR5174 EDB, Toulouse, France
(3)
Present Address: Case Western Reserve University School of Medicine, Cleveland, USA
(4)
Cornell Institute of Host Microbe Interactions and Disease, Cornell University, Ithaca, USA
(5)
Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras, Portugal

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