Skip to main content

Advertisement

  • Research article
  • Open Access

A Go-type opsin mediates the shadow reflex in the annelid Platynereis dumerilii

BMC Biology201816:41

https://doi.org/10.1186/s12915-018-0505-8

©  Tessmar-Raible et al. 2018

  • Received: 21 November 2017
  • Accepted: 12 March 2018
  • Published:

Abstract

Background

The presence of photoreceptive molecules outside the eye is widespread among animals, yet their functions in the periphery are less well understood. Marine organisms, such as annelid worms, exhibit a ‘shadow reflex’, a defensive withdrawal behaviour triggered by a decrease in illumination. Herein, we examine the cellular and molecular underpinnings of this response, identifying a role for a photoreceptor molecule of the Go-opsin class in the shadow response of the marine bristle worm Platynereis dumerilii.

Results

We found Pdu-Go-opsin1 expression in single specialised cells located in adult Platynereis head and trunk appendages, known as cirri. Using gene knock-out technology and ablation approaches, we show that the presence of Go-opsin1 and the cirri is necessary for the shadow reflex. Consistently, quantification of the shadow reflex reveals a chromatic dependence upon light of approximately 500 nm in wavelength, matching the photoexcitation characteristics of the Platynereis Go-opsin1. However, the loss of Go-opsin1 does not abolish the shadow reflex completely, suggesting the existence of a compensatory mechanism, possibly acting through a ciliary-type opsin, Pdu-c-opsin2, with a Lambdamax of approximately 490 nm.

Conclusions

We show that a Go-opsin is necessary for the shadow reflex in a marine annelid, describing a functional example for a peripherally expressed photoreceptor, and suggesting that, in different species, distinct opsins contribute to varying degrees to the shadow reflex.

Keywords

  • behaviour
  • opsins
  • non-visual
  • marine
  • Platynereis dumerilii
  • annelid
  • shadow reflex
  • peripheral photoreceptors
  • evolution

Background

The shadow reflex is a defensive withdrawal behaviour shared between many sessile and sedentary organisms. Well-described examples include different species of bivalves, which swiftly close their shell or retract their siphons [1]. Detailed attention has been paid to the spectrum that causes such a response in scallops [2], as well as the possible role of their mantle edge eyes in this response [3, 4]. Fan worms (Sabellidae and Serpulidae) exhibit a pronounced shadow response, withdrawing into their opaque tubes upon the disillumination of photoreceptors housed in their radiolar tentacles [5–7]. The primary function of these photosensitive structures, and those more recently documented in ark clams, is likely to be shadow detection [6], but so far research into the underlying molecular mechanisms of the shadow reflex has been limited due to the dearth of molecular tools available in these model organisms. Platynereis dumerilii, a molecularly slowly evolving [8, 9], morphologically archetypical marine nereidid [10], and distant annelid relative of sabellid fan worms [11–13], with its expansive genetic toolkit [14] and observable, well-characterised shadow reflex [15], represents a useful model to understand the molecular mechanisms and evolution of this behaviour. Platynereis dumerilii is a predominantly nocturnal animal, but the animals also exhibit movement and exploratory behaviour during the day, anchoring their trunks inside protective tubes whilst occasionally reaching out with their heads, likely to survey the environment and to feed [16]. Sudden darkness or a passing shadow leads to a highly reproducible longitudinal contraction of the body, which retracts the head and tail within the confines of the tube [15].

The photoreceptor molecules responsible for governing the shadow response are unknown, though two distinct protein subtypes represent potential shadow reflex light receptors. On the one hand, accumulating evidence suggests that a ciliary-type opsin (c-opsin) mediates the shadow reflex of fan worms, and c-opsins exist in Platynereis dumerilii [17, 18]. On the other hand, the first member of the Go-opsin class (belonging to the animal Tetraopsins group [19]) was identified and characterised in the mantle-edge eyes of the scallop, Patinopecten yessoensis [20]. Scallops are known for their distinct shadow reflex [2] and predator avoidance responses, thought to be mediated by their mantle-edge eyes [6, 21]. Go-opsins hence represent good photoreceptor candidates for the shadow response in this group. Go-opsin1 in Platynereis larvae has been shown to be responsible for the phototactic response to light [22], a useful function during their short, free-swimming pelagic stage. Platynereis larvae typically settle at the bottom at approximately 1 week of age and spend most of their entire remaining life in and around self-spun tubes. Sudden changes of surrounding conditions, such as light, lead to an immediate retraction of the worm into its home tube. Herein, we aim to quantitatively assess the shadow reflex in Platynereis dumerilii and use genetic tools to ascertain which photoreceptive proteins and anatomical regions of the organism are required for this behavioural response.

Results

The shadow response in Platynereis dumerilii is wavelength dependent and maximally activated by 500 nm light

We first established a shadow reflex paradigm assayable under chromatically variable light sources, consisting of a 2 s period of complete darkness (Fig. 1a, Additional files 1 and 2A, B). Under white light, the worms reproducibly perform their recognisable behaviour upon sudden loss of illumination (Fig. 1b). Further analyses using LEDs with different specific maximal emission spectra (‘monochromatic light’; Additional file 2) revealed that the Platynereis shadow reflex is wavelength dependent and maximally triggered by approximately 500 nm, or cyan, light (Fig. 1b), with a mean Shadow Response Success Rate (SRSR) comparable to that of white light. We find significantly decreased SRSRs under 520 and 470 nm and a virtually non-existent shadow reflex under 400 and 590 nm light, forming a distinct photoexcitation curve for the response (Fig. 1b). It should be noted that, whilst our 500 , 520 and 590 nm light conditions have comparable intensities (1 × 1012 photons/cm2/s), the 400 and 470 nm light conditions have an approximately 10-fold greater intensity (3 × 1013 photons/cm2/s; Additional file 2C, D), but still exhibit strongly decreased shadow responses in wild-type animals (Fig. 1b).
Fig. 1
Fig. 1

Wavelength dependence of the adult Platynereis shadow response and impaired shadow reflex in Go-opsin1−/− animals. a Schematic of light regime used for the Shadow Reflex Assay. Following a 60 min acclimatisation period of constant light, 12 ‘shadow stimuli’, consisting of 2 s periods of abrupt darkness, occur between 60 s intervals of light. Stimulation of foraging behaviour refers to the addition of Spinach-conditioned artificial seawater 15 min prior to the first shadow (see also Additional file 1: Movie S1 and Additional file 2). b Efficiency of the Shadow Reflex in wildtype adult Platynereis dumerilii subjected to shadows under white light (black, n = 74) and five different monochromatic light conditions, 400 nm (purple, n = 74), 470 nm (blue, n = 74), 500 nm (cyan, n = 74), 520 nm (green, n = 56) and 590 nm (orange, n = 44). Whiskers in the box plot indicate the range of scores; means are indicated with a cross whilst individual scores are plotted as diamonds. Data from 7 experimental days are combined. c Efficiency of the Shadow Reflex in sibling Go-opsin1+/+ (black, n = 30), Go-opsin1Δ8/+ (blue, n = 30) and Go-opsin1Δ8/Δ8 (red, n = 30) adult Platynereis dumerilii under five different light conditions. Whiskers in the box plots indicate the range of scores; means: cross, individual scores: diamonds. Significance scores (** < 0.01, *** < 0.001, **** < 0.0001) represent P values of Mann–Whitney U tests comparing wildtype, heterozygous and homozygous siblings

Additional file 1:

Movie S1. Shadow reflex assay infrared recording showing 35 wild type worms subjected to three subsequent white light shadow stimuli with the refractory period between each shadow included. A white circle in the upper left corner signifies light ON and no white circle signifies light OFF. Immediately upon loss of the light stimulus, animals are graded binarily as described in Methods. For each stimulus, green circles indicate animals scored 1 for a successful response, whilst magenta circles indicate animals scored 0 for no response. Indicator circles appear three seconds prior to loss of light. (MP4 1662 kb)

Go-opsin1 is required for the shadow response in adult Platynereis dumerilii

The spectral dependence of the Platynereis shadow response (Fig. 1b) matches closely with the action spectrum of Pdu-Go-opsin1, whose Lambdamax is approximately 498 nm [22], and we show that Go-opsin1 expression indeed persists in adult Platynereis (Fig. 2). Considering that adult Platynereis are predominantly benthic organisms and thus rarely engage in phototactic swimming behaviours, the role of Go-opsin1 in adult worms has remained enigmatic. We made use of worms carrying a previously characterised mutation in the Pdu-Go-opsin1 gene, which results in a premature stop codon and likely complete loss-of-function [22]. We tested homozygous mutant worms against heterozygous mutants and sibling wildtypes in our shadow response paradigm, and found that the loss of Pdu-Go-opsin1 significantly reduces the SRSR under white light (P < 0.0003), 500 nm light (P < 0.0001) and 520 nm light (P < 0.0036) (Fig. 1c), demonstrating Go-opsin1 dependency. Our data also suggests a Go-opsin1 protein level dependence, as the mean SRSRs of Go-opsin1 +/− animals fall between those of their wildtype and homozygous siblings under white, 500 nm and 520 nm light (Fig. 1c), emulating the protein level dependence of the phototactic response present in Platynereis larvae [22].
Fig. 2
Fig. 2

Expression domains of Go-opsin1 in adult Platynereis dumerilii. a–e Differential Interference Contrast images of adult Platynereis dumerilii (aligned anterior–posterior:top–bottom) stained by whole mount in situ hybridisation for Go-opsin1 (purple) (Additional file 5). a Whole head overview with Go-opsin1 expression in cells aligned along the edge of the peristomal cirri (c). Adult eyes (ae), palps (p) and antennae (a) are also indicated. Ocular expression (b, c) and dorsal neuronal expression (d, e) are shown. 40× magnification of Go-opsin1 cells in the peristomal cirri (f) and anal cirri (i) are shown alongside corresponding sense probe negative controls (g; peristomal cirri, k; anal cirri). 63× magnification of an individual Go-opsin1-expressing cell is shown in the peristomal (h) and anal (j) cirri. Scale bars: 50 μm for a, b, d, i and k, and 20 μm for c, e, f, g, h and j. Black arrowheads: Go-opsin expressing cells

Besides the shadow response, we also re-evaluated the circalunar maturation timing of mutant worms versus wildtype siblings under monochromatic 500 nm light. Go-opsin+/− or −/− animals exhibited a normal circalunar maturation rhythm compared to their wildtype siblings (Additional file 3).

Cirri of adult Platynereis dumerilii express Go-opsin1 and are required for the shadow response

We next assessed via which cells Go-opsin1 mediates its shadow reflex function. Expression in Platynereis dumerilii larvae is limited to the larval and adult eyes and a neuron proximal to the ciliary photoreceptor cells of the brain [22]. We confirmed expression in the adult eyes and found an expanded domain proximal to the ciliary photoreceptors in the adult animals (Fig. 2b–e). We also identified novel expression domains in the adult worm’s peripheral structures, known as cirri (Fig. 2a, f–k). Eight peristomal cirri branch out from the lateral edge of the prostomium at the anterior end of the worm and two anal cirri extend from the posterior final segment. All 10 of these cirri possess Pdu-Go-opsin1-expressing single cells (Fig. 2a, f–k).

To assess whether the Platynereis cirri are required for the worms shadow response we assayed the shadow reflex efficacy of adult animals whose cirri (both anal and peristomal) had been surgically removed (Additional file 4). Our data show that cirri removal results in significantly decreased SRSRs under white (P = 0.023), 500 nm (P = 0.0467) and 520 nm (P = 0.0227) light (Fig. 3a), indicating that the cirri play an integral role in Platynereis shadow detection, likely via Go-opsin1-expressing photoreceptive cells.
Fig. 3
Fig. 3

Requirement of the Platynereis shadow reflex on cirral extremities, but not rhabdomeric photoreceptor cells. a Shadow Response Success Rate (SRSR) of wildtype (black, n = 44) and sibling wildtype animals with peristomal and anal cirri removed (green, n = 44) under six different light conditions. Whiskers in the box plot indicate the range of scores; means are indicated with a cross whilst individual scores are plotted as diamonds. Significance scores (* < 0.05) of P values of Mann–Whitney U tests are displayed above boxes comparing Cirri+ and Cirri– animals (see also Additional file 4). b SRSR of untreated wildtype worms (black, n = 23), wildtype animals (purple, n = 23) and r-opsin1::egfp-f2a-ntr transgenic worms (cyan, n = 22) treated with 12 mM of metronidazole in 0.02% DMSO under six different light conditions. Whiskers in the box plot indicate the range of scores; means are indicated with a cross whilst individual scores are plotted as diamonds. No significant differences were found between tested groups, as calculated according to whether the difference between medians (calculated using Hodges–Lehmann non-parametric estimator) exceeded 95% confidence intervals

The r-opsin1-expressing cells of adult Platynereis dumerilii do not mediate the shadow reflex

Our data show that neither Go-opsin1 mutation nor removal of cirri leads to a complete loss of the shadow reflex (Figs. 1c and 3a). These findings strongly suggest that other photoreceptor(s) can compensate for Go-opsin1 and the loss of cirri. Pdu-r-opsin1 is a rhabdomeric opsin, prominently expressed in the worm’s eyes, as well as in several peripheral trunk photoreceptors [23]. We previously devised a method to specifically ablate all r-opsin1 + cells in a stable transgenic line expressing gfp and the bacterial enzyme nitroreductase under the r-opsin1 enhancer [24]. Given that r-opsin1 is prominently expressed in the rhabdomeric photoreceptors of the worm’s eyes, we reason that this ablation leads to dysfunctional eyes [23]. The complete absence of GFP fluorescence in treated transgenic worms was taken as an indicator for successful r-opsin1 + cell ablation. A higher variability of the SRSR (Fig. 3b) can be seen in metronidazole (mtz)-treated animals, but this is caused by the treatment itself as we find no significant difference between the SRSRs of r-opsin1::eGFP-f2a-ntr transgenic animals and equally treated wildtype controls (Fig. 3b), demonstrating that r-opsin1+ cells are not integral to the Platynereis shadow reflex.

Pdu-c-opsin2 excitation characteristics make it a potential secondary shadow photoreceptor

Our data indicate that another photoreceptor mediates shadow detection in addition to Go-opsin1, but that the known rhabdomeric photoreceptors of Platynereis can be excluded from this function (Fig. 3b). We therefore wondered if a ciliary-type opsin could be a suitable additional candidate, as a c-opsin is suggested to mediate the shadow reflex in fan worms [18]. Two c-opsins exist in Platynereis, c-opsin1 and c-opsin2 (please note that we refer to the originally identified Platynereis c-opsin [17], as c-opsin1, and to the additionally identified c-opsin as Pdu-c-opsin2, while a reversed nomenclature was used in Bok et al. [7]; Fig. 4a). Pdu-c-opsin1, is maximally photo-activated between 380 and 400 nm [25], and thus can be discounted from shadow detection due to a negligible wildtype shadow response at 400 nm light (Fig. 1b). However, a second Platynereis ciliary opsin, Pdu-c-opsin2, is present in head transcriptome data (Fig. 4a) [26]. No other c-opsins were identifiable from the worm’s transcriptome data nor genome traces. Sequences of opsins from other opsin-families exist, but they do not belong to any of the classes discussed for a role in the shadow reflex, and further expression or spectral characterisation has not been perfromed. We demonstrate here that Pdu-c-opsin2 exhibits a maximum absorption wavelength of 490 nm (Fig. 4b), making it a candidate for a secondary shadow detecting photoreceptor. Our data also show that Pdu-c-opsin2 is bistable, requiring > 580 nm light to revert to its approximately 490 nm activatable state (Fig. 4b). This quality would also allow the animal to respond relatively continuously to repeated stimuli.
Fig. 4
Fig. 4

Phylogeny and absorption spectrum of Platynereis c-opsin2. a Phylogenetic tree showing Platynereis c-opsin2 grouped with other ciliary opsins (see also Additional file 6). Bootstrap values are shown in percent (of 1000 bootstraps), bootstraps less than 50 and not pertaining directly to c-opsin2 are omitted. b Absorption spectrum of Platynereis c-opsin2 purified from COS-1 cells after incubation with 11-cis-retinal (black (1)). Spectra after repeated stimulation for 1 min by 440 nm light (red (2), yellow (4)) and for 30 s at > 580 nm (blue (3), purple (5)) are also shown as traces. The absorption maximum at ~490 nm is highlighted in green. (Inset) The absorption spectrum of Platynereis c-opsin2 in a wider wavelength range. Robust absorbance at ~280 nm is attributed to misfolded protein species

Discussion

The 2 s-long shadow stimulus of our reflex paradigm is produced by removing the connector plug from the illuminating LED circuit, thereby discontinuing the power (Additional file 1: Movie 1). Although this sudden cessation of light is not identical to a true moving shadow front generated by an object passing between a light source and the point of detection, this paradigm follows shadow reflex paradigms used for vertebrates and crustaceans [27], and a classical paradigm for Platynereis dumerilii [15]. The simple cessation of illumination is well reproducible and avoids any potentially startling vibrations, which may arise from passing physical objects above the worms.

The cyan range of light encompasses the wavelengths that typically penetrate water the deepest in coastal areas [28]. The maximal sensitivity of the Platynereis shadow reflex in this wavelength range is thus a highly valuable aspect of such a response, which is often crucial for survival.

Go-opsin1+ cells in Platynereis cirri (Fig. 2f–k), the mantle edge eyes of scallops [1] and radiolar eyes and ocelli of fan worms [6] are all positioned at the most distal extent of available extremities. Such peripheral placement of these photoreceptive structures is likely integral to the reliable detection of shadows, even if they do not pass directly over the cephalic or central region of the animal. The photoreceptive cells in the tentacular structures of both serpulids and sabellids have been thoroughly documented [7, 13], and might appear to be more elaborated versions of the simple cirral Go-opsin1 + photoreceptors in Platynereis dumerilii. However, several differences are apparent. Firstly, developmental data suggest that the anterior appendages of serpulids and sabellids, the radiolar tentacles, are developmentally homologous to Platynereis palpae and not the peristomal cirri [29]. Secondly, the light-sensitive structures of serpulids and sabellids are typically associated with pigment cells [13], which we do not observe in Platynereis (Fig. 2a, f–k). Finally, evidence so far points to the involvement of a ciliary-type opsin in fan worm peripheral photodetection [18]. Taken together, these findings suggest that the biological mechanisms conducting the shadow reflexes in Platynereis and fan worms have separate evolutionary origins. Pdu-c-opsin2 and Pdu-copsin1 can be detected in Platynereis head transcriptome data [26]; however, the expression levels of both genes are very low and, despite multiple attempts, were impossible to detect in adult Platynereis with the current whole mount in situ hybridisation technology.

Go-opsin1 is co-expressed in the r-opsin1+ cells within the adult eyes of Platynereis (Fig. 2b, c) [22]. Neither these r-opsin1+ cells in the eyes, nor the non-cephalic r-opsin1 cells in the parapodia [23] are necessary for the shadow reflex (Fig. 3b). This is congruous with research on sabellid worms whose rhabdomeric-type cephalic eyes, likely using rhabdomeric-type opsins, are suggested to play no part in shadow detection due to their occlusion within the animal’s opaque protective tube [13].

Conclusions

To conclude, our data strongly suggest that Go-opsin1 functions as one photoreceptor mediating the Platynereis shadow reflex, likely via its expression in single cells in the worm’s cirri. A second opsin, c-opsin2, possesses wavelength characteristics that would allow it to function as an additional receptor conducting this crucial and widely conserved survival reflex in Platynereis.

Methods

Experimental model and subject details

Platynereis dumerilii

All animals were bred and raised in the Marine Facility at the Max F. Perutz Laboratories in accordance with established protocols [30]. Worm cultures are maintained at 18 °C and a 16 h:8 h light:dark cycle. All wildtype animals used in all shadow assays presented here are of the PIN619512 strain background [14]. Platynereis dumerilii worms with an Δ8 base pair deletion in the Go-opsin1 gene were provided by the laboratory of Gaspar Jekely [22]. In order to obtain wildtype siblings for further analyses, these animals were outbred with the PIN619512 inbred line. Resulting heterozygous worms were subsequently incrossed, resulting in offspring with Go-opsin1 +/+ , Go-opsin1 +/− and Go-opsin1 −/− genotypes. Worms were genotyped by genomic DNA extraction from a single segment of clipped tail, which was then PCR amplified using standard primer pairs in exon 1 of Go-opsin1 (Additional file 5), given time to regenerate and subsequently used in the shadow response assay. Animals containing the transgene r-opsin1::egfp-f2a-ntr had been generated previously [23]. Platynereis animals used in the shadow reflex assay were at the immature stage of development, and therefore cannot be assessed for sex until later stages when sexual differentiation has occurred.

COS-1 mammalian cells

COS-1 cell lines were maintained at the Institute for Molecular Science, Okazaki, under standard conditions initially established by G. Khorana [31]. The cell line has not undergone authentication, but has been under continual maintenance by the Khorana, Farrens and Tsukamoto laboratories for approximately 30 years.

Method details

Light condition measurements

The behavioural chamber in which the shadow assay took place was a light-tight insulated box (60 cm × 60 cm × 80 cm) placed in a temperature controlled room (18 °C) on 2-cm thick dampening rubber mats to minimise vibrations. White light conditions were installed in this as described previously [16]. LED arrays (Winger Electronics GmbH & Co.KG) of additional monochromatic wavelengths were constructed and installed in the same configuration at the same distance from the sample, which lies 60 cm from the nearest LED (Additional file 2A, B). The spectrum and intensity of emitted light was measured with the ILT950 spectrometer from International Light Technologies Inc. (Peabody, USA) whose detector was placed at the prospective position of the sample. The light intensity was given in irradiance (μW/cm2/s) and was converted to photon flux (photons/cm2/s). We attempted to match the total photon flux between light conditions. However, there is a relative lack of chromatic specificity of commercial green/yellow (500–590 nm) compared to shorter wavelength monochromatic LEDs [32], and so lower intensities were used in this assay to avoid chromatic overlap.

Shadow reflex assay

From 96 h prior to the assay, immature Platynereis dumerilii of comparable sizes (~20 mm in length) were separated into individual wells of six-well dishes (Greiner Bio One International GmbH) and starved of food for the remainder of the assay. At 48 h prior to the assay, the animals were placed in individual hemispherical concave wells (Additional file 1: Movie 1 and Additional file 2B) (diameter = 35 mm, depth = 15 mm) of a custom-made 36-well clear plastic plate and kept under normal culture conditions to allow novel tube formation. Upon introduction to the behavioural chamber (Additional file 2A, B) at ZT5 on the day of assay, the wells were refilled with fresh artificial seawater (ASW) before the assay. The assay was repeated for all six different light conditions (Additional file 2C, D) on each cohort of animals. The order in which the light conditions were presented was randomised for each new cohort of animals. The assay began with a 60-min acclimatisation period of exposure to the given ON light stimulus. After 45 min, each well was spiked with 50 μL of spinach-conditioned ASW to encourage foraging behaviour and make grading of the shadow response more apparent. For the shadow light regime, 12 shadow stimuli, each consisting of 2 s of sudden complete darkness (OFF), were given. These shadows were each separated by 60 s of normal (ON) light conditions to avoid desensitisation to the stimuli (Fig. 1a). Video recording of worm behaviour in both ON and OFF light conditions was facilitated as described previously [16], using an infrared (IR) (λ = 990 nm) LED array (Roschwege GmbH) illuminating the behavioural chamber and an IR high-pass filter restricting the video camera. Video was recorded continuously at 15 frames per second for the duration of the shadow stimuli.

Cirri removal

For the cirri dependence assay, size-matched immature wildtype sibling worms were collected. Half were randomly selected to undergo cirri removal and the other half were not. At 2 days prior to assay onset, all animals (both cut and uncut) were anaesthetised by submersion in an isotonic MgCl2/ASW solution (7.5% w/v in ddH2O mixed 1:1 with ASW). ‘Cut’ animals were placed dorsally on a glass cover slide and their two anal cirri and eight peristomal cirri were surgically removed using fine scalpel blades (Swann Morton Ltd.) and tungsten carbide needles (Fine Science Tools Ltd.) (adapted from [33]). Note that care was taken to avoid causing simultaneous damage to the palps or antennae (Additional file 4). Animals were then transferred to fresh ASW and allowed to recover for 1 h before being placed individually in hemispherical wells alongside their uncut siblings to prepare tubes for the shadow reflex assay. Upon starting the shadow assay 2 days after surgery, ‘cirri-removed’ animals had not begun to regrow their detached cirri (Additional file 4).

Whole mount in situ hybridisation

Expression of Pdu-Go-opsin1 transcripts was localised in adult Platynereis dumerilii using an established whole mount in situ hybridisation protocol [34]. RNA probes were generated by in vitro transcription with T7 RNA Polymerase (New England Biolabs Inc. No. #M0251 L) incorporating digoxigenin-labelled UTPs (Roche Diagnostics GmbH, No. 11277073910). The probe template was generated by a PCR reaction conducted on immature wildtype Platynereis dumerilii cDNA using primers specific to the full coding sequences of the Go-opsin1 gene (Additional file 5). Images of Platynereis heads stained by in situ hybridisation were taken using a Zeiss Axioplan2 light microscope. Overall head images were taken under a 10× and 20× air objective lens and cellular images with a 40× and 63× oil immersion objective.

r-opsin1 + cell-specific ablation

Transgenic r-opsin1::eGFP-F2A-nitroreductase animals were pre-screened for strong GFP fluorescence, along with the same number of size-matched wildtype animals, and were subjected to mtz (Sigma, catalogue no. M1547) treatment as described previously [24]. Mtz treatment took place for 3 days at a concentration of 12 mM mtz dissolved in 0.2% DMSO in ASW. Following treatment, animals were allowed to recover for 2 days in ASW before being placed in the shadow assay wells. Complete absence of GFP fluorescence was taken as an indicator of a successful ablation procedure. The shadow assay therefore took place 4 days after mtz treatment, well within the 7 day period found to preclude regrowth of the r-opsin1+ cells [24].

In vitro opsin absorption assay

The in vitro opsin absorption assay was conducted as described by Tsukamoto et al. [25]. Briefly, native Platynereis c-opsin2 with the 1D4 tag (ETSQVAPA) on the C-termini was subcloned into the pMT vector. The construct was transiently expressed in mammalian COS-1 cells, incubated with 11-cis-retinal, and proteins were extracted with 1.25% DDM (Dojindo, Kumamoto, Japan), 20 mM HEPES, 140 mM NaCl, 0.25% cholesterol hemisuccinate (Sigma-Aldrich, St. Louis, MO, USA), 25 mM Tris and 10% glycerol at pH 7.0. The extract was mixed with 1D4-agarose overnight, and transferred to Bio-Spin columns (Bio-rad, Hercules, CA, USA). The columns were washed with 0.05% DDM, 2 mM ATP, 1 M NaCl, 3 mM MgCl2, 0.01% cholesterol hemisuccinate, 1 mM Tris and 10% glycerol in PBS, and subsequently washed with 0.05% DDM, 140 mM NaCl, 0.01% cholesterol hemisuccinate, 1 mM Tris, 10% glycerol and 20 mM HEPES at pH 7 (buffer A). The 1D4 tagged c-opsin2 was eluted with buffer A containing 0.45 mg/mL of 1D4 peptide (TETSQVAPA) (TOYOBO, Osaka, Japan). Absorption spectra of purified Platynereis c-opsin2 were recorded with a Shimadzu UV-2450 spectrophotometer (Shimadzu, Kyoto, Japan). During the spectral measurement, the samples were kept at 10 °C.

Phylogeny

The sequence alignment and tree calculation was performed using CLC Main Workbench, version 7.7.1. The alignment settings were as follows: gap open costs 10, gap extension costs 1, end gap costs as any other, very accurate alignment; settings tree: method UPGMA, protein distance measure: Kimura protein, 1000 bootstraps. The dataset supporting this tree is included in Additional file 6, as well as accession numbers and source organisms of protein sequences used.

Quantification and statistical analysis

Following assay completion, behaviour was analysed manually and individual animals were scored binarily (1 = withdrawal reflex, 0 = no reaction) for each shadow stimulus given. The withdrawal reflex is specifically defined as a shortening of total body length via retraction of the anterior and/or posterior end. If the animal is in the process of undulation associated with swimming or passive ventilation, this reflex can behaviourally appear as a sudden straightening of the body (Additional file 1: Movie 1). Prior to the assay, animals were placed in wells at random by a colleague with genotypes hidden from the behavioural analyser, and were revealed only once scoring had been conducted blindly. Individual SRSRs were then calculated according to the equation below:
$$ Shadow\ Reflex\ Success\ Rate\ (SRSR)=\frac{\# of\ successful\ shadow\ responses}{total\# of\ shadow s\ given} $$

Due to the fact that the number of successful shadow responses is always an integer value, SRSRs are an inherently discrete variable and thus the data takes on a stratified appearance. Statistical analysis and construction of box and whisker plots were conducted using Graphpad Prism 7.03 [35], where boxes represent interquartile range. Individual biological replicates are shown as diamonds in all cases. Datasets are approximately continuous (with 12 potential discrete values), unpaired and similarly distributed. Accordingly, significant differences between groups were calculated using two-tailed Mann–Whitney U tests with a P value threshold of 0.05. The Benjamini–Hochburg correction for multiple testing was applied to these tests in Fig. 1b, where each dataset was tested twice. For Fig. 3b, the lack of statistical significance between groups was calculated by assessment of whether the difference between pseudo-medians, calculated using Hodges–Lehmann non-parametric estimator, exceeds 95% confidence intervals. Under any single condition in every figure, SRSRs were pooled from at least three separate independent experiments. Precise values of n and the statistical parameters used in each figure are provided in the accompanying figure legends.

Abbreviations

ASW: 

artificial seawater

mtz: 

metronidazole

Pdu : 

Platynereis dumerilii

SRSR: 

shadow response success rate

Declarations

Acknowledgements

The authors wish to thank the Tessmar-Raible and Raible labs for constructive discussion and feedback, Andrij Belokurov and Margaryta Borysova for maintaining the Platynereis cultures, and Dr Yuji Furutani (Institute for Molecular Science) for help with the in vitro opsin absorption assay. We also thank Juliane Zantke and loopbio GmbH for their support in establishing the behaviour chamber used in the experiments.

Funding

TA was supported by a PhD fellowship of the Boehringer Ingelheim Foundation (https://www.bifonds.de/fellowships-grants/phd-fellowships.html). KTR is supported by grants from the University of Vienna (research platform “Rhythms of Life”), the FWF (http://www.fwf.ac.at/en/) START award (#AY0041321) and research project grant (#P28970), and the European Council under the European Community’s Seventh Framework Programme (FP7/2007-2013) ERC Grant Agreement 337011. HT is supported by JST, PRESTO (JPMJPR1787) and the Center for the Promotion of Integrated Sciences of SOKENDAI.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Availability of data and materials

All data generated or analysed during this study are included in this published article and its supplementary information files. The sequence for Pdu-c-opsin2 has been deposited in Genbank: MG182639.

Authors’ contributions

TA and KT-R conceived the experiments and wrote the manuscript. KT-R constructed the c-opsin2 phylogenetic tree and advised on progress throughout. TA conducted experiments pertaining to the shadow reflex assay, whole mount in situ hybridisation, cell ablation, light condition calibrations, animal culturing, analysed the data and constructed the figures. HT performed the opsin absorption assay, analysed the absorption data and wrote the methods section thereof. MG generated and donated the Go-opsin1 mutant Platynereis line. VBVR significantly contributed to the establishment of the behaviour chamber with the appropriate lighting conditions for SRSR recording used for the experiments. All authors read and approved the final manuscript.

Ethics approval

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Max F. Perutz Laboratories, University of Vienna, Campus Vienna Biocenter, Dr. Bohr-Gasse 9/4, 1030 Vienna, Austria
(2)
Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science, Okazaki 444-8585, Japan
(3)
Department of Structural Molecular Science, SOKENDAI (The Graduate University for Advanced Studies), Hayama, Kanagawa 240-0193, Japan
(4)
Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO), Kawaguchi, Saitama 332-0012, Japan
(5)
Max Planck Institute for Developmental Biology, Paul-Ehrlich Straße 20, 72076 Tübingen, Germany
(6)
Research Platform ‘Rhythms of Life’, University of Vienna, 1030 Vienna, Austria

References

  1. Wilkens LA. Primary inhibition by light: a unique property of bivalve photoreceptors. Am Malacol Bull. 2008;26:101–9.View ArticleGoogle Scholar
  2. Cronly-Dillon JR. Spectral sensitivity of the scallop Pecten maximus. Science. 1966;151:346–7.View ArticleGoogle Scholar
  3. Land MF. Activity in the optic nerve of Pecten maximus in response to changes in light intensity, and to pattern and movement in the optical environment. J Exp Biol. 1966;45:83–99.PubMedGoogle Scholar
  4. Buddenbrock W, Moller-Racke I. Uber den Lichtsinn von Pecten. Pubbl della Stn Zool di Napoli. 1953;24:217–45.Google Scholar
  5. Nicol JAC. Responses of Branchiomma Vesiculosum (Montagu) to photic stimulation. J Mar Biol Assoc U K. 1950;29:303–20.View ArticleGoogle Scholar
  6. Nilsson DE. Eyes as optical alarm systems in fan worms and ark clams. Philos Trans R Soc B. 1994;346:195–212.View ArticleGoogle Scholar
  7. Bok MJ, Porter ML, Ten Hove HA, Smith R, Nilsson D-E. Radiolar eyes of serpulid worms (Annelida, Serpulidae): structures, function, and phototransduction. Biol Bull. 2017;233:39–57.View ArticlePubMedGoogle Scholar
  8. Raible F, Tessmar-Raible K, Osoegawa K, Wincker P, Jubin C, Balavoine G, et al. Vertebrate-type intron-rich genes in the marine annelid Platynereis dumerilii. Science. 2005;310:1325–6.View ArticlePubMedGoogle Scholar
  9. Tessmar-Raible K, Arendt D. Emerging systems: between vertebrates and arthropods, the Lophotrochozoa. Curr Opin Genet Dev. 2003;13:331–40.View ArticlePubMedGoogle Scholar
  10. Bullock TH, Horridge GA. Structure and Function in the Nervous System of Invertebrates. San Francisco: W.H. Freeman; 1965. p. 4–125, 661–791.Google Scholar
  11. Özpolat BD, Bely AE. Developmental and molecular biology of annelid regeneration: a comparative review of recent studies. Curr Opin Genet Dev. 2016;40:144–53.View ArticlePubMedGoogle Scholar
  12. Weigert A, Helm C, Meyer M, Nickel B, Arendt D, Hausdorf B, et al. Illuminating the base of the annelid tree using transcriptomics. Mol Biol Evol. 2014;31:1391–401.View ArticlePubMedGoogle Scholar
  13. Bok MJ, Capa M, Nilsson DE. Here, There and everywhere: the radiolar eyes of fan worms (Annelida, Sabellidae). Integr Comp Biol. 2016;56:784–95.View ArticlePubMedGoogle Scholar
  14. Zantke J, Bannister S, Rajan VBV, Raible F, Tessmar-Raible K. Genetic and genomic tools for the marine annelid Platynereis dumerilii. Genetics. 2014;197:19–31.View ArticlePubMedPubMed CentralGoogle Scholar
  15. Evans SM. Habituation of the withdrawal response in Nereid Polychaetes. 2. Rates of habituation in intact and decerebrate worms. Biol Bull. 1969;137:105–17.View ArticleGoogle Scholar
  16. Zantke J, Ishikawa-Fujiwara T, Arboleda E, Lohs C, Schipany K, Hallay N, et al. Circadian and circalunar clock interactions in a marine annelid. Cell Rep. 2013;5:99–113.View ArticlePubMedPubMed CentralGoogle Scholar
  17. Arendt D, Tessmar-Raible K, Snyman H, Dorresteijn AW, Wittbrodt J. Ciliary photoreceptors with a vertebrate-type opsin in an invertebrate brain. Science. 2004;306:869–71.View ArticlePubMedGoogle Scholar
  18. Bok MJ, Porter ML, Nilsson D. Phototransduction in fan worm radiolar eyes. Curr Biol Elsevier. 2017;27:R698–9.View ArticleGoogle Scholar
  19. Ramirez DM, Pairett AN, Pankey MS, Serb JM, Speiser DI, Swafford A, et al. The last common ancestor of most bilaterian animals possessed at least nine opsins. Genome Biol Evol. 2016;8(12):3640–52.PubMedPubMed CentralGoogle Scholar
  20. Kojima D, Terakita A, Ishikawa T, Tsukahara Y, Maeda A, Shichida Y. A novel GO-mediated phototransduction cascade in scallop visual cells. J Biol Chem. 1997;272:22979–82.View ArticlePubMedGoogle Scholar
  21. Morton B. The function of pallial eyes within the Pectinidae, with a description of those present in Patinopecten yessoensis. Geol Soc Spec Publ. 2000;177:247–55.View ArticleGoogle Scholar
  22. Gühmann M, Jia H, Randel N, Veraszto C, Bezares-Caldero LA, Michiels NK, et al. Spectral tuning of phototaxis by a Go-opsin in the rhabdomeric eyes of Platynereis. Curr Biol. 2015;25:2265–71.View ArticlePubMedGoogle Scholar
  23. Backfisch B, Veedin-Rajan VB, Fischer RM, Lohs C, Arboleda E, Tessmar-Raible K, et al. Stable transgenesis in the marine annelid Platynereis dumerilii sheds new light on photoreceptor evolution. Proc Natl Acad Sci U S A. 2013;110:193–8.View ArticlePubMedGoogle Scholar
  24. Veedin-Rajan VB, Fischer RM, Raible F, Tessmar-Raible K. Conditional and specific cell ablation in the marine annelid Platynereis dumerilii. PLoS One. 2013;8:1–11.View ArticleGoogle Scholar
  25. Tsukamoto H, Chen I, Kubo Y, Furutani Y. A ciliary opsin in the brain of a marine annelid zooplankton is UV- sensitive and the sensitivity is tuned by a single amino acid residue. J Biol Chem. 2017;292:12971–80.View ArticlePubMedGoogle Scholar
  26. Backfisch B, Kozin VV, Kirchmaier S, Tessmar-Raible K, Raible F. Tools for gene-regulatory analyses in the marine annelid Platynereis dumerilii. PLoS One. 2014;9:e93076.View ArticlePubMedPubMed CentralGoogle Scholar
  27. Cameron AT, Donoghue CHO. Retinal reflexes of narcotized animals to sudden changes of intensity of illumination. Biol Bull. 1922;42:217–33.View ArticleGoogle Scholar
  28. Fischer A, Dorresteijn A. The polychaete Platynereis dumerilii (Annelida): a laboratory animal with spiralian cleavage, lifelong segment proliferation and a mixed benthic/pelagic life cycle. BioEssays. 2004;26:314–25.View ArticlePubMedGoogle Scholar
  29. Orrhage L. On the structure and homologues of the anterior end of the polychaete families Sabellidae and Serpulidae. Zoomorphology. 1980;168:113–68.Google Scholar
  30. Hauenschild C, Fischer A. Platynereis dumerilii. Mikroskopische Anatomie. Großes Zool Prakt. 1969;10b:1–54.Google Scholar
  31. Oprian DD, Molday RS, Kaufman RJ, Khorana HG. Expression of a synthetic bovine rhodopsin gene in monkey kidney cells. Proc Natl Acad Sci U S A. 1987;84:8874–8.View ArticlePubMedPubMed CentralGoogle Scholar
  32. Dekens MPS, Foulkes NS, Tessmar-Raible K. Instrument design and protocol for the study of light controlled processes in aquatic organisms, and its application to examine the effect of infrared light on zebrafish. PLoS One. 2017;12:1–15.View ArticleGoogle Scholar
  33. Hofmann DK. Prostomial regeneration in the polychaete Platynereis dumerilii (Audouin et Milne-Edwards) (Annelida). Wilhelm Roux’s Arch Dev Biol. 1975;177:309–22.View ArticleGoogle Scholar
  34. Tessmar-Raible K, Steinmetz PRH, Snyman H, Hassel M, Arendt D. Fluorescent two-color whole mount in situ hybridization in Platynereis dumerilii (Polychaeta, Annelida), an emerging marine molecular model for evolution and development. BioTechniques. 2005;39:460–4.View ArticlePubMedGoogle Scholar
  35. GraphPad Software Inc. GraphPad Prism version 7.00 for Windows. La Jolla: GraphPad Software Inc.; 2017.Google Scholar

Copyright

© Tessmar-Raible et al. 2018

Advertisement

BMC Biology

ISSN: 1741-7007

Contact us