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Entering an era of dynamic structural biology…
© Orville et al. 2018
- Published: 31 May 2018
A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX). They apply their method to resolve in near atomic detail the cleavage and inactivation of the antibiotic ceftriaxone by a β-lactamase enzyme from Mycobacterium tuberculosis. Their work demonstrates the general applicability of time-resolved crystallography, from which dynamic structures, at atomic resolution, can be obtained.
See research article: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-018-0524-5.
AMO is supported by Diamond Light Source and a Strategic Award from the Wellcome Trust and the Biotechnology and Biological Sciences Research Council (grant 102593).
The author read and approved the final manuscript.
The author declares that he has no competing interests.
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