Mechanical force-induced morphology changes in a human fungal pathogen

Charles Puerner; Nino Kukhaleishvili; Darren Thomson; Sebastien Schaub; Xavier Noblin; Agnese Seminara; Martine Bassilana; Robert A. Arkowitz

doi:10.1186/s12915-020-00833-0

Research article
Open Access
Published: 11 September 2020

Mechanical force-induced morphology changes in a human fungal pathogen

BMC Biology volume 18, Article number: 122 (2020) Cite this article

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Abstract

Background

The initial step of a number of human or plant fungal infections requires active penetration of host tissue. For example, active penetration of intestinal epithelia by Candida albicans is critical for dissemination from the gut into the bloodstream. However, little is known about how this fungal pathogen copes with resistive forces upon host cell invasion.

Results

In the present study, we have used PDMS micro-fabrication to probe the ability of filamentous C. albicans cells to penetrate and grow invasively in substrates of different stiffness. We show that there is a threshold for penetration that corresponds to a stiffness of ~ 200 kPa and that invasive growth within a stiff substrate is characterized by dramatic filament buckling, along with a stiffness-dependent decrease in extension rate. We observed a striking alteration in cell morphology, i.e., reduced cell compartment length and increased diameter during invasive growth, that is not due to depolarization of active Cdc42, but rather occurs at a substantial distance from the site of growth as a result of mechanical compression.

Conclusions

Our data reveal that in response to this compression, active Cdc42 levels are increased at the apex, whereas active Rho1 becomes depolarized, similar to that observed in membrane protrusions. Our results show that cell growth and morphology are altered during invasive growth, suggesting stiffness dictates the host cells that C. albicans can penetrate.

Background

Polar tip growth, in which extension is limited to the apical surface, enables walled cells such as fungi and plants to explore their environment for nutrients and mating partners, while maintaining their surface to volume ratio [1]. Campas and Mahadevan [2] have derived simple scaling laws for cell geometry and identified a single dimensionless parameter that is sufficient to describe variation in the shape of tip growing cells using turgor pressure, cell wall elastic properties, and secretion rate. However, little is known with respect to the response of tip growing cells to mechanical stress. There are five fundamental types of mechanical stress: tension, compression, shear, torsion, and bending. Human and plant fungal pathogens can penetrate host tissue, and it is likely that they encounter compressive stress upon penetration and subsequent invasive growth. In addition to tissue penetration, growth within a spatially confined environment is critical for infection and successful dissemination of such fungal pathogens.

Fungal pathogens take advantage of different strategies to interact with their environment, of which tip growth is a common theme. Penetration of host tissue is critical for both human and plant fungal pathogens and requires not only the generation of sufficient force but also adhesion to the host cells to counter this force [3, 4]. Fungal pathogens have turgor pressures in the MPa range [3, 4], and for human fungal pathogens, this turgor pressure exceeds host cell resistance to penetration. Such host cells have elastic moduli that are in the 1–100 kPa range [5,6,7], although the critical stress for material rupture is determinant. Both the human fungal pathogens Candida albicans [8] and Aspergillus fumigatus [9] can actively penetrate host tissue, which is a critical step in the infection process [10,11,12,13,14]. Previous studies have revealed that C. albicans can invade cells via host-induced endocytosis and/or active penetration [8]. C. albicans invasion of the intestinal epithelia (small intestinal enterocytes) occurs almost exclusively by active penetration [8, 15, 16], whereas both endocytosis and active penetration are important for invasion of the oral epithelia [17]. However, even with oral epithelia, at the early stages of infection, active penetration is the major route for tissue invasion [17]. Hence, a better understanding of active penetration should provide insight into the initial step of tissue damage for mucosal infections. Translocation of C. albicans through intestinal epithelial layers is facilitated by the fungal peptide toxin candidalysin [16, 18]. Previous studies have shown that C. albicans hyphal tips are asymmetrically positioned during growth on a stiff surface, i.e., a “nose down” morphology, and that perpendicular growth and contact to a stiff topographical ridge (less than the hyphal radius) results in an indentation of the ridge [19].

To investigate the relationship between substrate stiffness and C. albicans penetration and invasive growth, we have used micro-fabrication, together with time-lapse microscopy. We show that there is a threshold for penetration that corresponds to a stiffness of ~ 200 kPa and that invasive growth within a stiff substrate is characterized by dramatic filament buckling along with a stiffness-dependent decrease in extension rate. Nonetheless, a small percentage of cells are able to invade 200 kPa PDMS, suggesting that these cells may play a key role in infection, similar to that of the persister cells in biofilms. Furthermore, we observed a striking alteration in cell morphology during invasive growth, which is not due to depolarization of active Cdc42, but rather occurs at a substantial distance from the site of growth, as a result of mechanical forces. Our data reveal that in response to mechanical forces, C. albicans has increased active Cdc42 at the apex while active Rho1 is depolarized, similar to what is observed in PDGF-induced fibroblast membrane protrusions [20].

Results

Monitoring Candida albicans filamentous growth in micro-fabricated chambers

To investigate C. albicans hyphal growth, we took advantage of micro-fabrication approaches using the elastomer polydimethylsiloxane (PDMS) that, in particular, have been reported as single-cell force sensors for fission yeast cells [21]. We generated PDMS arrays with approximately 10⁵ microchambers, which were cylindrical in shape with a diameter of 10 μm, a depth of 5 μm, and 15 μm spacing between adjacent chambers (Fig. 1a). C. albicans cells in micro-fabricated PDMS chamber arrays were visualized with inverted microscopes; imaging was carried out through an upright array of 150–200-μm-thick PDMS. Figure 1b shows an XZ confocal reflectance scan through the PDMS microarray with the chambers and media at the top (highest position) and the coverslip below for support (a zoom of chambers is shown in Fig. 1c). C. albicans cells were mixed with fetal calf serum, added to the PDMS array, incubated for ~ 1 h, and subsequently, filamentous growth was followed over time (Fig. 1d). With low-stiffness PDMS (a high polymer to cross-linker ratio of 40:1) we observed two predominant filamentous growth modes: non-invasive growth on the PDMS surface and invasive growth within PDMS (Fig. 1e, f). By examination of the focal plane of the PDMS surface and the fungal filaments, using DIC optics, we were able to distinguish between non-invasive (surface) and invasive growth, referring to whether the filament tip is on or within the PDMS, respectively. Invasive growth was also confirmed by labeling the PDMS surface and filamentous cells (see below). Furthermore, we observed that the blastospore (round cell) portion of the filamentous cells, which grew in the microchambers, pushed back against the chamber wall upon PDMS filament penetration and the filament frequently buckled within PDMS, presumably due to the resistive force during growth within the elastomer (Figs. 1f and 2a). These results indicate that, in addition to having ideal optical properties, PDMS is compatible with C. albicans filamentous growth.

Growth modes depend on substrate stiffness

We followed C. albicans filamentous growth in PDMS of different stiffness, i.e. the extent to which an object resists deformation in response to an applied force, by varying the ratio of polymer to cross-linker. We observed two main growth modes from cells initially in chambers depending on PDMS stiffness: invasive growth, which predominated with less stiff PDMS (40:1) (Fig. 2a) and dramatic bending in the stiffer PDMS (30:1) chambers, which was predominantly subapical (Fig. 2b, top panel). In contrast to Schizosaccharomyces pombe [21], extensive deformation of the chambers was not observed with C. albicans, which we attribute to the different sizes, geometries, and growth modes of these fungi; fission yeast has a radius of ~ 2 μm, compared to C. albicans hyphal filaments with a radius of ~ 1 μm [22] (and our observations), resulting in a greater than 4-fold difference in the cross-sectional area. At most, a slight chamber deformation was observed with C. albicans, as cells frequently popped out of the chambers comprised of stiff PDMS or penetrated this material, when less stiff. Occasionally, at intermediate PDMS stiffness, we observed filamentous cells growing on the PDMS surface that appeared to be probing the surface with a “nose down” growth (Fig. 2b, bottom panel), as similarly observed [19]. This type of growth was suggestive of the filaments attempting to penetrate into PDMS and, consistent with this, we observed these filaments buckling and/or bending subapically at each attempt, prior to the tip popping out and forward. Buckling is defined as a sudden change in the shape of a component under load, i.e. change in the shape of the filament due to the physical forces it experiences. Subapical bending is, additionally, defined as a change in the direction of growth that results in curved filaments (Fig. 2c). In buckling, it is expected that the shape changes are largely reversed upon removal of the external forces, whereas in bending, the shape changes are not a result of the mechanical forces directly. Buckling can occur with a filament initially straight or bent/curved.

We next examined whether cells, initially in chambers, which were unable to invade, underwent bending. Figure 2d shows that as the stiffness of PDMS increased (from 50:1 to 30:1 PDMS to cross-linker), there was an increase in the percentage of cells undergoing bending, concomitant with a decrease in those invading PDMS. During invasive growth, we also frequently observed buckling of the filament (Fig. 2a red arrowheads, c), i.e. a growth-dependent curvature that typically occurred at the portion of the filament within PDMS. Figure 2e shows that such buckling was dependent on the PDMS stiffness, with over half of invasive filaments buckling in the two stiffest PDMS (40:1 and 35:1).

To determine the mechanical properties of the different PDMS preparations, we used dynamic mechanical analysis for which measurements were reproducible over a range of PDMS stiffness. Oscillating strain at a frequency of 10 Hz was applied to PDMS samples, and stress (σ)-strain (ε) curves were obtained (Additional file 1: Figure S1), from which Young’s modulus was determined (initial dσ/dε). Young’s modulus is the quantitation of the stiffness, i.e. the ratio stress/strain for a uniaxial load, where stress is the force per unit area and strain is the proportional deformation (change in length divided by original length) and is dimensionless. Figure 2f shows the Young’s modulus of different ratios of PDMS to cross-linker, which are in good agreement with published values [23,24,25,26]. The stiffness of lower ratio samples (10:1 and 20:1), intermediate ratios (30:1 to 40:1), and higher ratios (45:1 and 50:1) was similar to that of medical silicone implants [27], with a Young’s modulus of ~ 1 MPa; stiff tissues such as the myocardium [7], with a Young’s modulus of ~ 0.1–0.2 MPa; and less stiff tissues such as the epithelia [5, 6], with a Young’s modulus of ~ 40–70 kPa, respectively.

Penetration into and escape from PDMS

Given that active penetration is critical during the process of C. albicans epithelium invasion [10,11,12, 14], we examined in further detail this process in PDMS. Figure 3a shows a filamentous cell that penetrates PDMS after 4 min (II; note that I, not shown, is prior to the filament contacting the chamber wall); subsequently grows invasively within PDMS (III); deforms the adjacent chamber (IV), resulting in a dramatic invagination; and exits PDMS into the adjacent well at 2:04 (V), followed by penetration into the opposing chamber at 2:08 (VI) and subsequent invasive growth (2:12; VII). The resistive force revealed by buckling of the filament, as well as deformation of the initial chamber during invasive growth (III), likely increases upon deformation and subsequent piercing into the adjacent well (IV), as the portion of the filament within PDMS buckled during this time (1:22–2:02), resulting in an S-shaped filament (Fig. 3a). The tension on the filament was released upon exiting PDMS into the adjacent well (V), as the tip of the filament appears to jump forward (2:04). The resistive force from the final step of growth (VII) also resulted in buckling of the filament (portion in the well) leading to an M shape (2:42–3:00). This escape from PDMS is analogous, in some respects, to filaments bursting out of a macrophage [28,29,30,31]. Here, the filament pushes into a circle resulting in a deformation that does not require expansion of the surface area but rather local invagination of the chamber, which is easier to detect (Fig. 3a). Indeed, such a bursting out of PDMS was observed a number of times, and Additional file 1: Figure S2 shows such examples in different PDMS stiffness (40:1, 110 kPa; 35:1, 150 kPa; and 30:1, 250 kPa).

In order to better visualize the invasive growth within PDMS during these different steps, we followed cells in which GFP was targeted to the plasma membrane [32], by confocal spinning disk microscopy acquisition over a range of z-positions. Figure 3b and c show a typical time-lapse acquisition in which the analysis of the cell outline did not reveal a substantial change in the shape of the filament tip during invasive growth and bursting into the next well (Fig. 3c, d; Additional file 1: Figures S3A and S3B). Indeed, the radius of the curvature of the cell tip was identical to that of surface-growing cells, and there were no changes upon burst out of PDMS. Buckling of the filament was evident upon invasive growth and occurred over 35–45 min, prior to the appearance of a septum (Fig. 3b (red arrowheads) and Fig. 4a, two examples). Analyses of the angle of the filament at which the septum formed ultimately, indicate that during invasive growth, cytokinesis occurs the majority of the time after the filament buckles (Fig. 4b).

To analyze the physical constraints during penetration, invasive growth, and tip escape from the PDMS matrix, we established a physical experimental model, which consisted of a steel probe that mimics the filament shape, continuously advancing up to and into a cylinder of PDMS of different stiffness (Fig. 5a, b). The steel probe tip approximated the shape of the filament tip (Fig. 5c) with a radius of curvature (± 45°) of 1.1 μm when normalized to the hyphal filament, compared to that of 1.0 ± 0.1 μm for the surface and invasively growing hyphal filaments. Figure 5d shows the probe prior to PDMS rupture and subsequent to exiting from the PDMS. Experiments were carried out with a range of probe displacement rates encompassing that of the filament extension rates (~ 0.3 μm/min [33]), when scaled down to the filament diameter. Figure 6a shows an example of such a force versus displacement curve. The initial phase of increasing force corresponds to the elastic compression of PDMS (F_{elast compr}; analogous to growth stage II, Fig. 3a), culminating in the force required to break the PDMS surface, F_crit. The next phase corresponds to the extension within PDMS, analogous to invasive growth, F_invas (analogous to growth stage III, Fig. 3a) culminating in PDMS exit (F_exiting; analogous to the end of growth stage IV, Fig. 3a). The final phase corresponds to when the end of the probe has emerged from the PDMS (F_out; analogous to growth stage V, Fig. 3a). Figure 6b shows the F_crit from the physical probe experiments as a function of PDMS stiffness. Filaments are able to penetrate PDMS of ratio 35:1, for which we measured F_crit of ~ 7 N with a 1-mm-diameter probe. Scaled to the diameter of a hyphal filament, this would correspond to a force of ~ 31 μN, indicating that hyphal filaments generate forces larger than 31 μN to penetrate PDMS. Scaling to the diameter of a hyphal filament was done assuming a constant critical stress, given the filament diameter is several orders of magnitude greater than the PDMS mesh size, and was calculated by taking the ratio of the metal probe/filament radius squared. Furthermore, our results indicate that at a stiffness of 200 kPa, for which little PDMS penetration is observed (Fig. 2d), the F_crit is ~ 8 N with a 1-mm-diameter probe, i.e. ~ 35 μN when scaled to a hyphal filament, which would correspond to the growth stalling force.

Resistive force affects hyphal extension and morphology

The buckling of the filaments, as well as the deformation of the PDMS wells during invasive growth, indicated that these filaments were responding to resistive force, whose magnitude we have measured in the physical model. The percentage of cells that penetrate PDMS is dependent on Young’s modulus (Fig. 2d, f), and analyses of percent of PDMS invasion at two stiffness values indicate that the threshold for invasion is between 120 and 200 kPa (Fig. 7a). Hence, to investigate the effects of resistive force on filamentous growth, we determined the length of the filaments over time from cells growing on and within PDMS in this range of stiffness. Figure 7b shows that cells extend at a constant rate, which is reduced by ~ 30% within PDMS from an average of 0.28 ± 0.07 μm/min (n = 29) for surface growth to 0.19 ± 0.05 μm/min (n = 32) when filaments were growing within PDMS with a stiffness of ~ 100 kPa (Fig. 7c). This filament extension rate was further reduced to 0.15 ± 0.08 μm/min (n = 23) upon growth in PDMS with a stiffness of ~ 150 kPa (Fig. 7c). To confirm that the reductions in extension rate during invasive growth were not due to the substantial changes in the z-position of the growing filament apex, cells were grown in PDMS chambers that had been stained with fluorescent ConA and z-section images were projected onto the XZ plane (Fig. 7d). These projections show that the filaments grow slightly downward in PDMS, below the bottom of the chamber, with maximally 5 μm displacement in the z-axis for a 25-μm filament, resulting in at most a 2% reduction in extension rate upon projection in the XY plane. In contrast, Fig. 8a shows that the mean filament extension rate of cells grown on the surface is not dependent on the substrate’s stiffness, with indistinguishable rates on PDMS with Young’s modulus from 100 to 200 kPa. Furthermore, the extension rate of invasive growth normalized for that of surface growth from each experiment correlates with substrate stiffness (Fig. 8b). Extrapolation to the y-intercept, where the invasive extension rate, equals the surface rate indicates a substrate stiffness of ~ 20 kPa, suggesting that during filamentous growth on PDMS, the cells experience this resistive force from adhesion. Consistently, the surface extension rate was slightly reduced compared to that in liquid media (0.26 ± 0.09 μm/min compared to 0.32 ± 0.01 μm/min; p = 0.001) (Fig. 8a). Of note, very few cells invaded PDMS at 200 kPa, on average ~ 5% (Fig. 7a), but, strikingly, the filament extension rate for these “escapers” was similar to that of less stiff PDMS, raising the attractive possibility that these cells may play a critical role in tissue invasion.

The difference in filament extension rate could either be due to an overall reduction in cell growth or a reduction in polarized, apical growth. To differentiate between these possibilities, we determined the length and diameter of compartments (between 2 septa) for cells growing on the surface and within PDMS (100 kPa). Figure 9a and b show that the compartment length decreased ~ 30%, from 24.6 ± 3.0 μm (n = 100) for surface growing cells to 16.6 ± 1.8 μm (n = 120) during invasive growth, and the filament diameter increased concomitantly from 2.1 ± 0.2 μm for surface growing cells to 2.5 ± 0.2 μm during invasive growth. As a result, the compartment volume remained constant (83 ± 17 μm³ for surface growing cells compared to 80 ± 18 μm³ for invasively growing cells), indicative of altered polarized growth. Consistently, analyses of filamentous cells grown within stiffer PDMS (150 kPa) revealed a further decrease in compartment length (14.6 ± 2.5 μm) and an increased diameter (2.8 ± 0.3 μm). This altered morphology is dependent on growth against a resistive force in PDMS as the diameter in the part of the filament outside PDMS was similar to that of surface growing cells (Fig. 10a). In addition to the comparison of cells growing on the surface and within PDMS, we examined the relatively rare occurrence of cells transitioning between these growth modes. Figure 10b and c show an example of such a transition, in which the extension rate is reduced during invasive growth in PDMS and increases exiting PDMS. Measurements of the filament diameter just before and after the filament exited PDMS revealed an increased filament diameter during invasive growth that was significantly reduced upon exiting PDMS (2.7 ± 0.2 μm compared to 2.3 ± 0.3 μm, Fig. 10d).

Reduced filament extension rate in response to a resistive force suggested that similar effects could be observed in cells undergoing non-invasive, dramatic subapical bending in chambers of stiff PDMS (Additional file 1: Figure S4A). Additional file 1: Figure S4B shows that, in such conditions, there was indeed a dramatic reduction in filament extension rate with an average (over 100–150 min) of 0.10 ± 0.01 μm/min. Surface growth rates were constant over time, whereas extension rates of cells undergoing dramatic subapical bending decreased concomitantly with the cell filling the well (Additional file 1: Figure S4C); initial rates of extension were 3-fold reduced from surface growth, and these were further reduced 3-fold after 2 h of growth. Due to the complex geometries during such a growth mode, we were unable to determine the resistive force that the filament experiences while it fills up the chamber; however, the initial extension rate is similar to that of filaments growing invasively in 150 kPa PDMS.

Determination of the effective turgor pressure

From the comparison of extension rates within PDMS of different stiffness (Fig. 7c), we determined the effective turgor pressure in C. albicans hyphae, using the viscoplastic growth model [21]. This determination makes use of F_invas values, measured from the physical model (Fig. 6), after scaling these forces from a cylinder with a radius of 0.5 mm to that of 1.04 μm. In order to correctly extrapolate the macroscopic measurements to the microscopic scale of filamentous cells, we analyzed the physical forces at play. The mode of extension during hyphal growth and in this physical experimental model is different, as new material is incorporated into the hyphal tip, i.e. growth occurs via apical extension, whereas in the physical experimental model, the probe is pushed into the PDMS from the back. Given that only a small portion of the filamentous cell apex extends in the PDMS, we removed the contribution from friction/adhesion due to the displacement of a 1-mm-diameter probe within PDMS by subtracting the F_out value from the F_invas (Fig. 6c). These corrected F_invas values, i.e. F_in, were largely independent of probe displacement rates over a range equivalent to cell filament extension rates when scaled down (0.2–0.4 μm/min). We used the equation that was established for S. pombe by Minc and colleagues;

$$ \frac{V_{(F)}}{V_o}=\left(1-\frac{F_{\left(\mathrm{PDMS}\right)}}{\pi {R}^2\Delta P}\right) $$

(1)

V_(F) and V_o are the filament extension rates within PDMS and on the surface, respectively; F_(PDMS) is the resistive force of PDMS during filament displacement within this material (F_in); R is the filament radius; and ∆P is the effective turgor pressure. The F_(PDMS) was 1.5 ± 0.7 N and 0.7 ± 0.3 N at PDMS to cross-linker 35:1 (Young’s modulus of 150 kPa) and 40:1 (Young’s modulus of 100 kPa), respectively; scaling to the size of the hyphal filament (radius 1.04 μm for surface growth) yielded 6 ± 3 μN and 3.2 ± 1.4 μN, respectively. From these values, we determined the effective turgor pressure, ∆P, to be 6 ± 3 MPa and 2 ± 1 MPa in these two conditions, respectively. Given that the hyphal filament diameter increases during invasion (radius 1.42 μm at Young’s modulus of 150 kPa and 1.24 μm at Young’s modulus of 100 kPa), the calculated ∆P are 3.1 ± 1.5 MPa and 1.5 ± 0.7 MPa, suggesting that the hyphal turgor pressure is 1–3 MPa. This value for turgor pressure is within the range reported both for planktonic and biofilm C. albicans cells, ~ 1.2 MPa [19] and ~ 2 MPa [34], respectively, as well as S. pombe, 0.85–1.5 MPa [21, 35]. Nonetheless, it must be noted that these values are effective turgor pressure. In other words, ∆P is the turgor pressure exceeding the critical stress needed to deform the cell wall [21]. Hence, a combination of local compartment turgor pressure alteration, difference in cell wall deformability, or potentially finer adjustments in tip geometry may play important roles in penetration and invasion.

Resistive force affects cell polarity

The change in morphology during invasion, resulting in shorter and wider cells, could be explained by tip growth becoming more isotropic in response to a resistive force, raising the possibility that cell polarity is adversely affected. In S. pombe, it was observed that reducing the growth rate chemically, genetically, or mechanically destabilized active Cdc42 polarization, a cell polarity master regulator [36]. To investigate whether cell polarity was altered in hyphal filaments growing invasively in PDMS, we examined the distribution of active Cdc42 (Cdc42•GTP), using a CRIB-GFP reporter [37]. Surprisingly, we observed a striking increase in polarized active Cdc42 at the filament tip throughout invasive growth, compared to surface growth (Fig. 11a, b). We determined, using a tailor-made MATLAB program, that this results from an increase in the concentration of Cdc42•GTP at the tip, rather than an alteration in the position of the maximum signal or spread of active Cdc42 further down the filament (Additional file 1: Figure S5A-D). These results suggest that, in response to a resistive force, there is an increase in cell polarization, perhaps reflecting a direct response to such external forces. We speculate that this higher level of active Cdc42 during invasive growth is due to the increased recruitment of the Cdc42 activator, Cdc24 [38]. We next examined active Rho1, as cell wall stress mediated by the cell surface mechanosensors Wsc1/Mid2 results in Rho1 depolarization in S. cerevisiae [39, 40]. Figure 11c and d show that, in contrast to the increase in tip localized active Cdc42, active Rho1 is depolarized during invasive growth. We attribute this depolarization of active Rho1 to the mechanical properties of PDMS, which are likely to impose a uniform force over the hyphal filament surface, in addition to the resistive force in response to the tip extension.

The increase in tip-localized active Cdc42 during invasive growth suggests that the increase in filament diameter does not result from growth becoming more isotropic in response to resistive force. To examine whether this morphological change results from mechanical forces, we compared cell morphology over time during growth on the surface and within PDMS. Figure 12a and b show that the relative filament diameter (D1) was not altered during surface growth (mean diameter 2.26 ± 0.15 μm initially compared to 2.44 ± 0.06 μm after 2 h growth), in contrast to the invasive growth where there was a striking increase (2.38 ± 0.22 μm initially compared to 2.98 ± 0.15 μm, p < 0.0001). Specifically, the diameter of the filament compartment increased even > 10 μm back from the tip (Fig. 12b). This ~ 25% increase in diameter during invasive growth could either occur upon tip growth or subsequent to tip growth. The hyphal tip diameter was constant over 2 h of invasive growth and only slightly wider than that of cells growing on the surface (2.53 ± 0.11 μm compared to 2.24 ± 0.09 μm; p = 0.0003) (Fig. 12c, d). In contrast, Fig. 12d shows that there was a significant difference between the mean diameter at the tip of the apical cell and that of the cell proximal to the apical cell during invasive growth (mean proximal cell diameter 2.99 ± 0.17 μm; p < 0.0001). Together, these results indicate that relatively small changes in the tip morphology are not sufficient to explain the altered morphology of the filament, back from the tip, which are due to external mechanical forces.

Discussion

We used PDMS micro-fabrication to probe the relationship between substrate stiffness and growth of C. albicans filamentous cells. Below a stiffness threshold of ~ 200 kPa, C. albicans can penetrate and grow within PDMS. The chemical inertness of this polymer, as well as the observed well deformation, suggest that turgor pressure-driven active penetration is critical for this invasive growth. C. albicans filamentous growth within a stiff substrate is characterized by dramatic filament buckling, which correlates with the position of cell division sites, in addition to a stiffness-dependent decrease in extension rate. Growth within a substrate also resulted in a striking alteration in morphology, i.e. reduced cell compartment length and increased diameter. Our results reveal that changes in morphology are not due to a depolarization of active Cdc42, but rather to the mechanical forces from the substrate.

Growth behavior as a function of substrate stiffness

Substrate stiffness determines whether C. albicans will grow on a surface or within it, below a threshold corresponding to a stiffness of 200 kPa. During growth within PDMS, a substantial fraction of filaments buckle, with greater than 50% buckling in a substrate with a Young’s modulus of ~ 100 kPa. Cells trapped in stiff (200 kPa or greater) microchambers undergo dramatic subapical bending. While both filament buckling and subapical bending depend on filament extension, the former occurs at least 5–10 μm away from the apex whereas the latter occurs at the filament tip. In a number of cases, filaments buckled as the tip (within PDMS) approached a well, followed by partial release upon penetration into this well. This bursting out event is similar, in some respects, to damaging of or escaping from host cells, such as macrophages [28,29,30,31].

Despite the estimated resistive force during invasive growth of 3–10 μN at the hyphal scale, we did not observe dramatic changes in the filament tip shape. Interestingly, the location of cell division sites appeared to be affected by filament buckling, in that there was a high correlation between the division site location and the site of buckling. Septins have been shown to be enriched preferentially at locations of high curvature in Ashbya gossypii hyphal filaments, i.e. at branch points [41]. An attractive possibility is that the location of the cell division site at the buckle, or vice versa, may minimize physical stress or damage to the filament.

Effects of substrate stiffness on invasive growth

Filament extension rate is reduced, and cell compartment length decreases with increasing substrate stiffness; however, cell volume is largely unaffected during growth within 100 kPa PDMS, due to an increase in filament diameter. These results suggest that the overall growth rate is unaffected in this condition, raising the possibility that the cell tip growth area is altered by resistive force. Given that altering growth in S. pombe by chemical, genetic, or mechanical means, results in destabilization of the active Cdc42 cluster at the growth site, we investigated the distribution of this key GTPase during invasive growth. Surprisingly, the distribution of active Cdc42 was not altered during invasive growth, but rather there was an increase in the concentration of active Cdc42 at the tip. In contrast, active Rho1, which is critical for glucan synthesis, was less polarized and observed throughout the filament during invasive growth. Together, these results suggest that, during invasive growth, cells sense resistive force from the PDMS and polarization increases at the tip, while remodeling their cell walls throughout the filament to counter this resistive force and/or the increase in turgor pressure. A likely possibility is that this resistive force is sensed by the mechano-sensing pathway, via a module composed of the mucin Msb2 and the cell wall protein Sho1 [42] critical for S. cerevisiae survival during compressive stress. In this yeast, Msb2 localizes to sites of growth and binds active Cdc42 [43], and cell wall stress has been shown to depolarize Rho1, as well as to hyperactivate this GTPase [39, 40] via the cell surface sensors, Wsc1 and Mid2. Indeed, S. cerevisiae cells sense compressive stress via Mid2, which localizes uniformly at the plasma membrane [44]. We speculate that the increased compression around the hyphal filament leads to depolarized active Rho1 via Mid2, in contrast to localized active Cdc42 via Msb2.

We have used a viscoplastic model for fungal growth that was originally established to describe cell shape control in plants [45, 46] and used, more recently, to analyze cell growth in fission yeast [21]. In such models, viscoplastic deformation of the cell wall underlies growth, which is driven by high turgor pressure. Specifically, the pressure (P) has to exceed the threshold plastic yield strain (P_c) of the cell wall—the cell growth rate is proportional to the wall strain that exceeds this threshold: $ {V}_o\propto \frac{\left(P-{P}_c\right)}{E_{\mathrm{CW}}} $. Hence, we have used Eq. (1) to derive the effective turgor pressure in C. albicans hyphae. Here, we used the differences in growth rates on PDMS surface and within PDMS, as well as an approximation of the external force. This latter force was calculated based on the displacement within PDMS of a 1-mm-diameter steel probe, minus the contribution from friction/adhesion and scaled to the hyphal diameter, assuming scaling with respect to the cross-sectional area and the absence of dramatic changes in geometry. From this analysis, we determined the effective turgor pressure (∆P = P − P_c) to be 1.5 and 3.1 MPa, in 100 kPa and 150 kPa PDMS, respectively. Either increasing filament diameter or decreasing extension rate results in a lower ∆P. The physical experimental model, however, does not take into account the geometry of the hyphal tip, which has a different curvature than the metal probe, which could result in an overestimation of the forces in the physical model. More precise measurements of forces relevant to hyphae will require a more accurate description of the hyphal tip size and geometry. One possibility is that, upon growth in stiffer PDMS, there is an increase in turgor pressure; our results are consistent with such a scenario as ∆P increases from 1.5 to 3.1 MPa, with an increase in PDMS stiffness from 100 to 150 kPa. This increase in turgor pressure could be responsible for the increased volume of the invasive cell compartment within 150 kPa PDMS (92 μm³ compared to 83 μm³ for surface growing cells, p = 0.045). Previous studies on cell wall expansion in S. pombe [35] have shown that $ \frac{\Delta P}{Y}=\frac{\left({R}^{\ast}\right)t}{R_1} $; where Y is the cell wall Young’s modulus; R^* is the expansion ratio in width ([R₁ – R₀]/R₀); R₁ and R₀ are the cell radius within the PDMS and on the surface, respectively; and t is the cell wall thickness (~ 0.2 μm). Assuming Y is constant at these two PDMS stiffness conditions, $ \frac{\Delta P}{Y}\left(35:1\right) $ is 1.7-fold greater than $ \frac{\Delta P}{Y}\left(40:1\right) $, consistent with the observed increase in turgor pressure we derived from the growth rate difference. The slightly reduced extension rate on PDMS surface compared to that in liquid, together with the extrapolation of normalized extension rate as a function of PDMS stiffness to where $ \frac{V_{(F)}}{V_o}=1 $, indicates that during growth on a surface, the hyphae experience a small resistive force, equivalent to growth within less stiff PDMS (~ 20 kPa), which we attribute to adherence/friction. It is likely that depending on the cellular surface, the contribution of adherence/friction will vary.

Cell morphology is dependent of substrate stiffness

Our results indicate that effects on cell morphology are only observed in filaments within the PDMS, suggesting that resistive forces from the PDMS lead to an alteration in morphology. Strikingly, during growth in the stiffest PDMS, we observed a progressive increase in compartment diameter, even when this compartment was more than 10 μm back from the hyphal tip. This could be due to the additional modification of the cell wall in this proximal compartment, i.e. resulting in a less stiff cell wall, an increase in turgor pressure, and/or mechanical deformation of the filament. Although we cannot rule out that the cell wall in this proximal compartment is less stiff during invasive growth, we favor the latter two possibilities, as the cell compartment volume increases and ~ 60% of invasively growing hyphae buckle in this PDMS stiffness (150 kPa). The dramatic alteration in filament morphology was not solely due to a widened tip, as it only increased slightly during invasive growth, compared to the proximal compartment that increased 25% more than the tip diameter. It is likely that these morphology changes are due to the mechanical forces from growing against a resistive substrate together with an increase in turgor pressure.

A number of fungal pathogens penetrate host tissues, including medically relevant C. albicans [8, 10, 12] and A. fumigatus [9], and plant pathogens [47, 48], including Colletotrichum sp. [49], Ustilago maydis [50], Magnaporthe sp. [51, 52], and Fusarium sp. [53]. For the latter plant pathogens, high turgor pressure is generated inside a specialized cell, called an appressorium, that generates pressures in excess of 8 MPa [51]. Blocking host cell endocytosis of the human fungal pathogens C. albicans [8, 15,16,17] and A. fumigatus [9] with Cytochalasin D has revealed that both of these fungi can enter epithelial tissue by active penetration. Young’s moduli for mammalian host cells are in the 1–100 kPa range. Hence, the effects we observe on filament extension rate and morphology are likely to be relevant during active penetration of epithelial cells, and it is attractive to speculate that turgor pressure, in particular the osmolytes critical for generating this force, might be a target for antifungal drugs.

Conclusions

Our results suggest that the stiffness of host cells dictates which cell type C. albicans can penetrate. Interestingly, even with stiffer PDMS (~ 200 kPa), we observed a small percentage of cells that were able to invade the substrate, suggesting these cells have specific properties that could be an advantage during epithelium invasion.

Methods

Strains, media, and genetic methods

Standard methods were used for C. albicans cell culture, molecular, and genetic manipulations as described. Derivatives of the BWP17 strain were used in this study and are listed in Table S1. Strains were grown in rich media (yeast extract peptone dextrose) at 30 °C for all experiments, and induction of filamentous growth was carried out with fetal calf serum (FCS) at 37 °C. Oligonucleotides and synthesized DNA used in this study are listed in Tables S2 and S3. pDUP5-mScarlet-Ct_Rac1 was generated by PCR amplification of CamScarlet with a unique 5′ AscI site and a 3′ CtRac1 followed by a unique MluI site (oligonucleotides CamScarletAscIp and yemChmCtRacMluI) and cloned into pDUP5-ADH1p-CibN-Ct_Rac1-ACT1t [33] resulting in pDUP5-ADH1p-CamScarlet-Ct_Rac1-ACT1t. The nucleotide sequence for RFP mScarlet [54] was codon optimized for C. albicans and commercially synthesized (Genscript). mScarlet was PCR amplified with unique PstI and AscI sites (oligonucleotides GA3CamScarPstIp and CamScarletmAscI) and cloned into a pFA-GFPγ-URA3 backbone [55] resulting in pFA-CamScarlet-URA3. The RID, CRIB, and Ct_Rac1 plasmids were linearized with StuI or NgoMIV and transformed into strains.

Micro-fabrication

Microchambers were fabricated using standard soft-lithography methods [56, 57]). Chambers were 5 μm deep and 10 μm in diameter with 15 μm spacing between them. The overall thickness of microchamber preparations was approximately 150 μm. PDMS microchambers of varying stiffness were generated by varying the ratios of polymer and cross-linker (Sylgard 184; Dow Corning). Polymer-cross-linker mixtures were spin coated (Laurell Technologies corporation) on molds (5 s 100 rpm followed by 50 s 500 rpm) to achieve ~ 150 μm thickness and then cured for 1 h at 60 °C PDMS (10:1). Thick PDMS frames were then placed on top of the partially cured chambers, which were baked for an additional 2 h at 90 °C, in order to peel the thin chambers off the mold. For viscoanalyzer measurements, 20 × 20 × 5 mm PDMS pieces were similarly cured and samples subjected to an amplitude of oscillation of 5–150 μm at 10 Hz frequency using a DMA3007000 Metravib 52 (Areva) with Dynaset20 software. Young’s moduli were calculated using the following formula, $ E=\frac{\sigma }{\varepsilon }=\frac{F\times h}{D\times l\times e} $; with F being the force, D the deformation amplitude, H the sample height, e the sample thickness, and l the sample length. For analysis of each PDMS preparation, measurements at 5 different deformation values (5–150 μm) were carried out, and the mean of 3 of these measurements was used for Young’s modulus determination. For indentation experiments, varying ratios of polymer and cross-linker were poured into two different sized cylindrical aluminum molds, 3.5 (d) × 1.75 cm (h) and 8 (d) × 2 cm (h), and cured as described above.

Physical model of penetration

Half of the PDMS cylinders were adhered to an aluminum plate with a 5-mm-diameter hole. For the other half of the PDMS cylinders, the bottom of the mold was removed such that they were fixed around their circumference. Cylindrical PDMS pieces from both conditions were indented in their center with a hemi-spherical 1-mm-diameter steel probe, which was highly polished. This probe tip was machined with a slight conical shape ending in a spherical cap to approximate a hyphal filament tip and had a 2-daN force sensor (Kistler), as well as a displacement sensor (Fastar Data Instruments) attached. This probe was extended using a moving deck with a motor (Cerclet) at speeds of 1.6–3.2 μm/s. Measurements were made on average 10 samples at each PDMS ratio. From force versus displacement curves, the following forces were derived: the maximum value was the F_crit, the mean of the first plateau following penetration F_invas, and the mean of the second plateau (following the exit of the PDMS) F_out. Forces were then averaged and scaled to the cross-sectional area of the hyphal filament. We assumed that in the physical model of penetration and in the C. albicans growth experiments, the PDMS behaved as a homogenous material, and hence, scaling in terms of size was carried out with a constant critical stress. This assumption is justified, as the diameter of the filament, ~ 2 μm, is 10³-fold greater than the PDMS mesh size, which is 2–3 nm with 40:1 PDMS. Hence, scaling of the force, as stress during rupture is constant, was achieved by dividing for the ratio of the metal probe/filament radii squared.

Microscopy sample preparation

PDMS microchambers were activated by plasma treatment (Harrick Plasma Cleaner) for 14 s at 500 mTorr on low setting submerged in DH₂O until usage. Prior to usage, PDMS samples were dried with nitrogen gas, treated with poly-d-lysine (1 mg/ml) and subsequently treated with concanavalin A (0.4 mg/ml), each incubated for 20 min followed by drying with a stream of nitrogen gas. Exponentially growing cells were mixed with FCS media (75% FCS, 0.6 × minimal media and 2% dextrose) and spotted onto the microchambers and were sealed with a coverslip. Typically, cells on PDMS microchambers were incubated for ~ 1 h at 37 °C prior to microscopy to initiate filamentation.

Microscopy and image analysis

Cells were imaged as described [33] using either spinning disk confocal microscopy or wide-field fluorescence microscopy with a UPLANAPO 1.2 NA × 60 or Plan-Neofluar 0.75 NA × 40 objectives, respectively. Images were acquired at indicated times, with 0.4 or 0.5 μm z-sections (13 to 24) to capture the entire filament. For growth depth experiments, 100 × 0.2 μm z-sections were acquired. For the analyses of extension rate, compartment morphology, tip morphology, and cell depth images were deconvolved with the Huygens Professional software version 18.04 (Scientific-Volume Imaging) with recommended settings and a signal to noise ratio of 10, and sum projections were used. Reflection images in XZ were acquired on an upright Leica DM5500 TCS SPE laser-scanning microscope (Leica Microsystems, Mannheim, Germany) equipped with a galvanometric stage, using an APO 0.3 NA × 10 objective. Reflection images were acquired in XZ reflection mode using a 488-nm laser and an 80/20 dichroic filter. Scale bars, unless otherwise indicated, are 5 μm.

Image analysis for extension rate, compartment/tip diameter, and active Rho GTPase polarization was carried out with Fiji (version 1.51) [58]. For the determination of the extension rate, the filament lengths (mother cell neck to filament tip) were determined over time using plasma membrane fluorescence signal and values are from a curve fit of minimally 1 h acquisition (> 12 time points). For liquid extension rates, cells induced with 50% FCS at 37 °C were fixed every 30 min and average cell lengths for each time point (0–90 min) were used. Compartment lengths were measured using the edge-to-edge fluorescence signal between two formed septa. Only invasive compartments that were entirely within the PDMS were used for analyses. Active Cdc42 and Rho1 polarization was determined using a fixed size ROI to quantitate the signal intensity at the tip and in a subapical region. The ratio of the tip to subapical signal was averaged over the time-lapse, and the mean for invasive cells was normalized to the mean ratio for non-invasive cells. The percentage of cells undergoing subapical bending within a chamber and filament buckling within PDMS was assessed using DIC images. For the determination of errors from values derived from multiple measurements, classical error propagation was carried out [59]. Statistical significance was determined with Student’s t test.

For the representation of 3D DIC images, an algorithm was applied to image z-stacks using a custom-developed MATLAB program called InFocus inspired from Extended Depth of Field plugin [60]. The program extracts from any 3D multi-channel acquisition, the local contrast per channel. From these maps, it determines the Z of highest contrast per pixel from one or a combination of channels and then extracts a smoothened plane to get a 2D multichannel image. Parameters can be adapted through the graphical user interface. For quantitation of filament tip curvature over time, we developed another MATLAB program called TipCurve with an intuitive interface dedicated for morphological analyses along the major axis of filamentous cells. For such analyses, 3D images were converted into 2D images by sum projection, and similar to the HyphalPolarity program [33], a backbone was extracted from images over time, but also the cell contour, specifically extracting the tip first, we estimate the extremity of the backbone subtracted by the tip radius. Then, we estimate the local curvature only along the tip (either − 45° to + 45° or − 90 to 90°) from the curvilinear function f(s) extracted from cell contour:

$$ C(s)=\frac{1}{R(s)}=\frac{f^{{\prime\prime} }(s)}{{\left(f\prime (s)\right)}^2} $$

The distribution of active Cdc42 and Rho1 at the filament tip was determined by TipCurve. For these analyses, images that had a polarized fluorescent signal along with a uniform cytoplasmic signal were used. The latter was used to identify the cell and extract the backbone. Then, an additive projection on the backbone was done to get kymograph curves per time point and per fluorescence channel. To estimate the distribution of intensity at the tip of Cdc42 and Rho1, we fitted the kymograph to estimate x_max, the distance of maximum of intensity from the tip and the decay of intensity above x_max as an exponential function. Both Infocus and TipCurve can be provided as executables on demand.

Availability of data and materials

All the data on which the conclusions of the paper are based are presented in the paper and its additional files.

References

1.
Campas O, Rojas E, Dumais J, Mahadevan L. Strategies for cell shape control in tip-growing cells. Am J Bot. 2012;99(9):1577–82.
PubMed Google Scholar
2.
Campas O, Mahadevan L. Shape and dynamics of tip-growing cells. Curr Biol. 2009;19(24):2102–7.
CAS PubMed Google Scholar
3.
Lew RR. How does a hypha grow? The biophysics of pressurized growth in fungi. Nat Rev Microbiol. 2011;9(7):509–18.
CAS PubMed Google Scholar
4.
Mendgen K, Hahn M, Deising H. Morphogenesis and mechanisms of penetration by plant pathogenic fungi. Annu Rev Phytopathol. 1996;34:367–86.
CAS PubMed Google Scholar
5.
Akhtar R, Sherratt MJ, Cruickshank JK, Derby B. Characterizing the elastic properties of tissues. Mater Today (Kidlington). 2011;14(3):96–105.
CAS Google Scholar
6.
Alonso JL, Goldmann WH. Feeling the forces: atomic force microscopy in cell biology. Life Sci. 2003;72(23):2553–60.
CAS PubMed Google Scholar
7.
Mathur AB, Collinsworth AM, Reichert WM, Kraus WE, Truskey GA. Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy. J Biomech. 2001;34(12):1545–53.
CAS PubMed Google Scholar
8.
Dalle F, Wachtler B, L’Ollivier C, Holland G, Bannert N, Wilson D, Labruere C, Bonnin A, Hube B. Cellular interactions of Candida albicans with human oral epithelial cells and enterocytes. Cell Microbiol. 2010;12(2):248–71.
CAS PubMed Google Scholar
9.
Bertuzzi M, Schrettl M, Alcazar-Fuoli L, Cairns TC, Munoz A, Walker LA, Herbst S, Safari M, Cheverton AM, Chen D, et al. The pH-responsive PacC transcription factor of Aspergillus fumigatus governs epithelial entry and tissue invasion during pulmonary aspergillosis. PLoS Pathog. 2014;10(10):e1004413.
PubMed PubMed Central Google Scholar
10.
Basmaciyan L, Bon F, Paradis T, Lapaquette P, Dalle F. Candida Albicans interactions with the host: crossing the intestinal epithelial barrier. Tissue Barriers. 2019;7(2):1612661.
PubMed PubMed Central Google Scholar
11.
Richardson JP, Ho J, Naglik JR. Candida-epithelial interactions. J Fungi (Basel). 2018;4(1).
12.
Swidergall M, Filler SG. Oropharyngeal candidiasis: fungal invasion and epithelial cell responses. PLoS Pathog. 2017;13(1):e1006056.
PubMed PubMed Central Google Scholar
13.
Westman J, Hube B, Fairn GD. Integrity under stress: host membrane remodelling and damage by fungal pathogens. Cell Microbiol. 2019;21(4):e13016.
CAS PubMed Google Scholar
14.
Wilson D, Naglik JR, Hube B. The missing link between Candida albicans hyphal morphogenesis and host cell damage. PLoS Pathog. 2016;12(10):e1005867.
PubMed PubMed Central Google Scholar
15.
Goyer M, Loiselet A, Bon F, L’Ollivier C, Laue M, Holland G, Bonnin A, Dalle F. Intestinal cell tight junctions limit invasion of Candida albicans through active penetration and endocytosis in the early stages of the interaction of the fungus with the intestinal barrier. PLoS One. 2016;11(3):e0149159.
PubMed PubMed Central Google Scholar
16.
Allert S, Forster TM, Svensson CM, Richardson JP, Pawlik T, Hebecker B, Rudolphi S, Juraschitz M, Schaller M, Blagojevic M, et al. Candida albicans-induced epithelial damage mediates translocation through intestinal barriers. mBio. 2018;9(3):e00915–18.
17.
Wachtler B, Citiulo F, Jablonowski N, Forster S, Dalle F, Schaller M, Wilson D, Hube B. Candida albicans-epithelial interactions: dissecting the roles of active penetration, induced endocytosis and host factors on the infection process. PLoS One. 2012;7(5):e36952.
PubMed PubMed Central Google Scholar
18.
Moyes DL, Wilson D, Richardson JP, Mogavero S, Tang SX, Wernecke J, Hofs S, Gratacap RL, Robbins J, Runglall M, et al. Candidalysin is a fungal peptide toxin critical for mucosal infection. Nature. 2016;532(7597):64–8.
CAS PubMed PubMed Central Google Scholar
19.
Thomson DD, Wehmeier S, Byfield FJ, Janmey PA, Caballero-Lima D, Crossley A, Brand AC. Contact-induced apical asymmetry drives the thigmotropic responses of Candida albicans hyphae. Cell Microbiol. 2015;17(3):342–54.
CAS PubMed Google Scholar
20.
Martin K, Reimann A, Fritz RD, Ryu H, Jeon NL, Pertz O. Spatio-temporal co-ordination of RhoA, Rac1 and Cdc42 activation during prototypical edge protrusion and retraction dynamics. Sci Rep. 2016;6:21901.
CAS PubMed PubMed Central Google Scholar
21.
Minc N, Boudaoud A, Chang F. Mechanical forces of fission yeast growth. Curr Biol. 2009;19(13):1096–101.
CAS PubMed PubMed Central Google Scholar
22.
Sevilla MJ, Odds FC. Development of Candida albicans hyphae in different growth media-variations in growth rates, cell dimensions and timing of morphogenetic events. J Gen Microbiol. 1986;132(11):3083–8.
CAS PubMed Google Scholar
23.
Brown XQ, Ookawa K, Wong JY. Evaluation of polydimethylsiloxane scaffolds with physiologically-relevant elastic moduli: interplay of substrate mechanics and surface chemistry effects on vascular smooth muscle cell response. Biomaterials. 2005;26(16):3123–9.
CAS PubMed Google Scholar
24.
Demichelis A, Pavarelli S, Mortati L, Sassi G, Sassi M. Study on the AFM force spectroscopy method for elastic modulus measurement of living cells. J Phys Conf Ser. 2013;459:012050.
Google Scholar
25.
Xie J, Zhang Q, Zhu T, Zhang Y, Liu B, Xu J, Zhao H. Substrate stiffness-regulated matrix metalloproteinase output in myocardial cells and cardiac fibroblasts: implications for myocardial fibrosis. Acta Biomater. 2014;10(6):2463–72.
CAS PubMed Google Scholar
26.
Wang Z, Volinsky A, Gallant ND. Crosslinking effect on polydimethylsiloxane elastic modulus measured by custom-built compression instrument. J Appl Polym Sci. 2014;131(22):41050.
Google Scholar
27.
Feng L, Li S, Feng S. Preparation and characterization of silicone rubber with high modulus via tension spring-type crosslinking. RSC Adv. 2017;7:13130–7.
CAS Google Scholar
28.
Lorenz MC, Bender JA, Fink GR. Transcriptional response of Candida albicans upon internalization by macrophages. Eukaryot Cell. 2004;3(5):1076–87.
CAS PubMed PubMed Central Google Scholar
29.
McKenzie CG, Koser U, Lewis LE, Bain JM, Mora-Montes HM, Barker RN, Gow NA, Erwig LP. Contribution of Candida albicans cell wall components to recognition by and escape from murine macrophages. Infect Immun. 2010;78(4):1650–8.
CAS PubMed PubMed Central Google Scholar
30.
Rudkin FM, Bain JM, Walls C, Lewis LE, Gow NA, Erwig LP. Altered dynamics of Candida albicans phagocytosis by macrophages and PMNs when both phagocyte subsets are present. mBio. 2013;4(6):e00810–3.
PubMed PubMed Central Google Scholar
31.
Westman J, Moran G, Mogavero S, Hube B, Grinstein S. Candida albicans hyphal expansion causes phagosomal membrane damage and luminal alkalinization. mBio. 2018;9(5):e01226–18.
32.
Vauchelles R, Stalder D, Botton T, Arkowitz RA, Bassilana M. Rac1 dynamics in the human opportunistic fungal pathogen Candida albicans. PLoS One. 2010;5(10):e15400.
PubMed PubMed Central Google Scholar
33.
Silva PM, Puerner C, Seminara A, Bassilana M, Arkowitz RA. Secretory vesicle clustering in fungal filamentous cells does not require directional growth. Cell Rep. 2019;28(8):2231–45 e2235.
CAS PubMed Google Scholar
34.
Desai JV, Cheng S, Ying T, Nguyen MH, Clancy CJ, Lanni F, Mitchell AP. Coordination of Candida albicans invasion and infection functions by phosphoglycerol phosphatase Rhr2. Pathogens. 2015;4(3):573–89.
CAS PubMed PubMed Central Google Scholar
35.
Atilgan E, Magidson V, Khodjakov A, Chang F. Morphogenesis of the fission yeast cell through cell wall expansion. Curr Biol. 2015;25(16):2150–7.
CAS PubMed PubMed Central Google Scholar
36.
Haupt A, Ershov D, Minc N. A positive feedback between growth and polarity provides directional persistency and flexibility to the process of tip growth. Curr Biol. 2018;28(20):3342–51 e3343.
CAS PubMed Google Scholar
37.
Corvest V, Bogliolo S, Follette P, Arkowitz RA, Bassilana M. Spatiotemporal regulation of Rho1 and Cdc42 activity during Candida albicans filamentous growth. Mol Microbiol. 2013;89(4):626–48.
CAS PubMed Google Scholar
38.
Bassilana M, Hopkins J, Arkowitz RA. Regulation of the Cdc42/Cdc24 GTPase module during Candida albicans hyphal growth. Eukaryot Cell. 2005;4(3):588–603.
CAS PubMed PubMed Central Google Scholar
39.
Delley PA, Hall MN. Cell wall stress depolarizes cell growth via hyperactivation of RHO1. J Cell Biol. 1999;147(1):163–74.
CAS PubMed PubMed Central Google Scholar
40.
Philip B, Levin DE. Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1. Mol Cell Biol. 2001;21(1):271–80.
CAS PubMed PubMed Central Google Scholar
41.
Bridges AA, Jentzsch MS, Oakes PW, Occhipinti P, Gladfelter AS. Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton. J Cell Biol. 2016;213(1):23–32.
CAS PubMed PubMed Central Google Scholar
42.
Delarue M, Poterewicz G, Hoxha O, Choi J, Yoo W, Kayser J, Holt L, Hallatschek O. SCWISh network is essential for survival under mechanical pressure. Proc Natl Acad Sci U S A. 2017;114(51):13465–70.
CAS PubMed PubMed Central Google Scholar
43.
Cullen PJ, Sabbagh W Jr, Graham E, Irick MM, van Olden EK, Neal C, Delrow J, Bardwell L, Sprague GF Jr. A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev. 2004;18(14):1695–708.
CAS PubMed PubMed Central Google Scholar
44.
Mishra R, van Drogen F, Dechant R, Oh S, Jeon NL, Lee SS, Peter M. Protein kinase C and calcineurin cooperatively mediate cell survival under compressive mechanical stress. Proc Natl Acad Sci U S A. 2017;114(51):13471–6.
CAS PubMed PubMed Central Google Scholar
45.
Boudaoud A. Growth of walled cells: from shells to vesicles. Phys Rev Lett. 2003;91(1):018104.
PubMed Google Scholar
46.
Lockhart JA. An analysis of irreversible plant cell elongation. J Theor Biol. 1965;8(2):264–75.
CAS PubMed Google Scholar
47.
Demoor A, Silar P, Brun S. Appressorium: the breakthrough in Dikarya. J Fungi (Basel). 2019;5(3).
48.
Ryder LS, Talbot NJ. Regulation of appressorium development in pathogenic fungi. Curr Opin Plant Biol. 2015;26:8–13.
CAS PubMed PubMed Central Google Scholar
49.
De Silva DD, Crous PW, Ades PK, Hyde KD, Taylor PWJ. Life styles of Colletotrichum species and implications for plant biosecurity. Fungal Biol Rev. 2017;31(3):155–68.
Google Scholar
50.
Matei A, Doehlemann G. Cell biology of corn smut disease-Ustilago maydis as a model for biotrophic interactions. Curr Opin Microbiol. 2016;34:60–6.
PubMed Google Scholar
51.
Howard RJ, Ferrari MA, Roach DH, Money NP. Penetration of hard substrates by a fungus employing enormous turgor pressures. Proc Natl Acad Sci U S A. 1991;88(24):11281–4.
CAS PubMed PubMed Central Google Scholar
52.
Tanaka E. Appressorium-mediated penetration of Magnaporthe oryzae and Colletotrichum orbiculare into surface-cross-linked agar media. FEMS Microbiol Lett. 2015;362(10):fnv066.
53.
Parry DW, Pegg GF. Surface colonization, penetration and growth of three Fusarium species in lucerne. Trans Br Mycol Soc. 1985;85(3):495–500.
Google Scholar
54.
Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, et al. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods. 2017;14(1):53–6.
CAS PubMed Google Scholar
55.
Zhang C, Konopka JB. A photostable green fluorescent protein variant for analysis of protein localization in Candida albicans. Eukaryot Cell. 2010;9(1):224–6.
CAS PubMed Google Scholar
56.
Minc N. Microfabricated chambers as force sensors for probing forces of fungal growth. Methods Cell Biol. 2014;120:215–26.
PubMed Google Scholar
57.
Whitesides GM, Ostuni E, Takayama S, Jiang X, Ingber DE. Soft lithography in biology and biochemistry. Annu Rev Biomed Eng. 2001;3:335–73.
CAS PubMed Google Scholar
58.
Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9(7):676–82.
CAS PubMed Google Scholar
59.
Bevington P, Robinson DK. Data reduction and error analysis for the physical sciences. New York: University of California: McGraw-Hill Education; 2003.
60.
Forster B, Van De Ville D, Berent J, Sage D, Unser M. Complex wavelets for extended depth-of-field: a new method for the fusion of multichannel microscopy images. Microsc Res Tech. 2004;65(1–2):33–42.
PubMed Google Scholar
61.
Wilson RB, Davis D, Mitchell AP. Rapid hypothesis testing with Candida albicans through gene disruption with short homology regions. J Bacteriol. 1999;181(6):1868–74.
CAS PubMed PubMed Central Google Scholar
62.
Bassilana M, Blyth J, Arkowitz RA. Cdc24, the GDP-GTP exchange factor for Cdc42, is required for invasive hyphal growth of Candida albicans. Eukaryot Cell. 2003;2(1):9–18.
CAS PubMed PubMed Central Google Scholar

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Acknowledgements

We thank J. Konopka for reagents, Y. Izmaylov, S. Bogliolo, O. Domenge, and S. Lachambre for the assistance and N. Minc for stimulating discussion.

Funding

This work was supported by the CNRS, INSERM, Université Nice-Sophia Antipolis, Université Côte d’Azur and ANR (ANR-15-IDEX-01, ANR-11-LABX-0028-01, and ANR-16-CE13-0010-01), and EU H2020 (MSCA-ITN- 2015-675407) grants and the Platforms Resources in Imaging and Scientific Microscopy facility (PRISM) and Microscopy Imaging Côte d’Azur (MICA).

Author information

Charles Puerner, Nino Kukhaleishvili, and Darren Thomson are co-first authors; order was determined by contribution to figures.

Affiliations

Université Côte d’Azur, CNRS, INSERM, Institute of Biology Valrose (iBV), Parc Valrose, Nice, France
Charles Puerner, Nino Kukhaleishvili, Darren Thomson, Sebastien Schaub, Martine Bassilana & Robert A. Arkowitz
Université Côte d’Azur, CNRS, Institute Physics of Nice (INPHYNI), Ave. J. Vallot, Nice, France
Nino Kukhaleishvili, Xavier Noblin & Agnese Seminara
Present Address: Manchester Fungal Infection Group, School of Biological Sciences, University of Manchester, Manchester, UK
Darren Thomson
Present Address: Sorbonne University, CNRS, Developmental Biology Laboratory (LBDV), Villefranche-sur-mer, France
Sebastien Schaub

Authors

Charles Puerner
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Nino Kukhaleishvili
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Darren Thomson
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Sebastien Schaub
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Xavier Noblin
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Agnese Seminara
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Martine Bassilana
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Robert A. Arkowitz
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Contributions

Conceptualization: X.N., A.S., M.B., and R.A.A. Methodology: C.P., N.K., D.T., S.S., X.N., and R.A.A. Software: S.S. Validation: X.N., A.S., and R.A.A. Formal analysis: C.P., N.K., A.S., and R.A.A. Investigation: C.P., N.K., D.T., and X.N. Data curation: S.S., A.S., and R.A.A. Writing—original draft: M.B. and R.A.A. Writing—review and editing: C.P., N.K., D.T., X.N., A.S., M.B., and R.A.A. Visualization: R.A.A. Supervision: X.N., M.B., and R.A.A. Project administration: X.N., M.B., and R.A.A. Funding acquisition: X.N., A.S., M.B., and R.A.A. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Xavier Noblin or Robert A. Arkowitz.

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Supplementary information

Additional file 2: Movie S1. Invasive growth and penetration into adjacent chamber. Cells grown with indicated stiffness PDMS and followed over time either by DIC optics or fluorescence of labeled with plasma membrane GFP.

Additional file 3: Movie S2. Invasively growing filaments have increased levels of active Cdc42 at the tip. False colored sum projections of cells expressing CRIB-GFP reporter for active Cdc42.

Additional file 1: Figure S1.

Strain versus stress dependence of PDMS. Analyses carried out using a Viscoanalyzer, with oscillation at 10 Hz of PDMS at cross-linker ratio of 40:1. Figure S2. Invasive growth and penetration into adjacent chamber in PDMS of different stiffness. DIC time-lapse experiments at indicated PDMS:cross-linker ratio and measured stiffness (Young’s modulus). The adjacent chamber is highlighted with a dotted yellow line and deformation of this chamber lasted ~40 min with 40:1 PDMS ratio and 80-90 min for the two stiffer PDMS substrates. Figure S3. The shape of filament tip is not substantially altered during invasive growth in PDMS. A) Radius of curvature over time is constant in surface and invasively growing cells. Radius of curvature with an arc of ± 90° or ± 45° at the filament tip. B) Shape of filament tip of surface growing cells over time. Cells were grown on PDMS (30:1; 250 kPa) and 31 × 5 min GFP sum projections were analyzed. Radius of curvature with ± 45° by indicated open lines and ± 90° indicated by solid lines. Figure S4. Cells confined within a stiff PDMS chamber have reduced filament extension rates. A) Constricted growth within a PDMS chamber. Typical time-lapse experiment using 160 kPa PDMS, with DIC images every 5 min shown. B) Filament extension rate within a stiff chamber is not linear. Filament length was determined from images every 5 min for ~ 2 h and GFP sum projections (n = 9 cells). C) Filament extension rate is substantially reduced as chamber fills up. Initial (filament length 10-20 μm) and final (filament length > 20 μm) extension rates were determined from fits to 6 × 5 min GFP sum projections. (colors represent individual cells). Bars indicate SD and **** p < 0.0001. Figure S5. Distribution of active Cdc42 is not altered during invasive growth. A) Schematic indicating fluorescence signal over the filament long axis. Quantitation of slope of Gaussian farthest from tip in red (Max Slope, in relative units), distance maximum signal to tip (x_max in μm), and half width half max of the Gaussian farthest from tip in red (x_Spread-x_max), i.e. the signal spread (Spread in μm). Signal is denoted by I and distance from tip by x. B) Distribution of active Cdc42 during surface and invasive filamentous growth. Experiment described in Figure 11a and 11b with the mean signal for each cell (colors represents individual cells), normalized to the mean signal for tip Cdc42•GTP in surface growing cells. Bars indicate SD. C) Distribution of active Cdc42 is not altered upon invasive growth. Relative maximum slope (left), distance from maximum signal to the tip (middle) and spread of signal (right) determined from 6-8 cells, using tailor-made Matlab program. Bars indicate SD; surface and invasive cells were not significantly different. D) Apical and subapical active Cdc42 signals are stable over time. Relative signals from apical and subapical region of sum projections, normalized to maximum invasive subapical signal.

Additional file 4: Table S1.

Strains used in the study [61, 62]. Table S2. Oligonucleotides used in the study. Table S3. Synthesized DNA used in the study.

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Puerner, C., Kukhaleishvili, N., Thomson, D. et al. Mechanical force-induced morphology changes in a human fungal pathogen. BMC Biol 18, 122 (2020). https://doi.org/10.1186/s12915-020-00833-0

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Received: 08 April 2020
Accepted: 22 July 2020
Published: 11 September 2020
DOI: https://doi.org/10.1186/s12915-020-00833-0

Keywords

Cell invasion
Mechanical force
Cell polarity
Cdc42
Cell morphology

Mechanical force-induced morphology changes in a human fungal pathogen

Abstract

Background

Results

Conclusions

Background

Results

Monitoring Candida albicans filamentous growth in micro-fabricated chambers

Growth modes depend on substrate stiffness

Penetration into and escape from PDMS

Resistive force affects hyphal extension and morphology

Determination of the effective turgor pressure

Resistive force affects cell polarity

Discussion

Growth behavior as a function of substrate stiffness

Effects of substrate stiffness on invasive growth

Cell morphology is dependent of substrate stiffness

Conclusions

Methods

Strains, media, and genetic methods

Micro-fabrication

Physical model of penetration

Microscopy sample preparation

Microscopy and image analysis

Availability of data and materials

References

Acknowledgements

Funding

Author information

Affiliations

Contributions

Corresponding authors

Ethics declarations

Ethics approval and consent to participate

Consent for publication

Competing interests

Additional information

Publisher’s Note

Supplementary information

Additional file 1: Figure S1.

Additional file 4: Table S1.

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About this article

Cite this article

Keywords

BMC Biology

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