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The complete chloroplast DNA sequence of the green alga Oltmannsiellopsis viridis reveals a distinctive quadripartite architecture in the chloroplast genome of early diverging ulvophytes



The phylum Chlorophyta contains the majority of the green algae and is divided into four classes. The basal position of the Prasinophyceae has been well documented, but the divergence order of the Ulvophyceae, Trebouxiophyceae and Chlorophyceae is currently debated. The four complete chloroplast DNA (cpDNA) sequences presently available for representatives of these classes have revealed extensive variability in overall structure, gene content, intron composition and gene order. The chloroplast genome of Pseudendoclonium (Ulvophyceae), in particular, is characterized by an atypical quadripartite architecture that deviates from the ancestral type by a large inverted repeat (IR) featuring an inverted rRNA operon and a small single-copy (SSC) region containing 14 genes normally found in the large single-copy (LSC) region. To gain insights into the nature of the events that led to the reorganization of the chloroplast genome in the Ulvophyceae, we have determined the complete cpDNA sequence of Oltmannsiellopsis viridis, a representative of a distinct, early diverging lineage.


The 151,933 bp IR-containing genome of Oltmannsiellopsis differs considerably from Pseudendoclonium and other chlorophyte cpDNAs in intron content and gene order, but shares close similarities with its ulvophyte homologue at the levels of quadripartite architecture, gene content and gene density. Oltmannsiellopsis cpDNA encodes 105 genes, contains five group I introns, and features many short dispersed repeats. As in Pseudendoclonium cpDNA, the rRNA genes in the IR are transcribed toward the single copy region featuring the genes typically found in the ancestral LSC region, and the opposite single copy region harbours genes characteristic of both the ancestral SSC and LSC regions. The 52 genes that were transferred from the ancestral LSC to SSC region include 12 of those observed in Pseudendoclonium cpDNA. Surprisingly, the overall gene organization of Oltmannsiellopsis cpDNA more closely resembles that of Chlorella (Trebouxiophyceae) cpDNA.


The chloroplast genome of the last common ancestor of Oltmannsiellopsis and Pseudendoclonium contained a minimum of 108 genes, carried only a few group I introns, and featured a distinctive quadripartite architecture. Numerous changes were experienced by the chloroplast genome in the lineages leading to Oltmannsiellopsis and Pseudendoclonium. Our comparative analyses of chlorophyte cpDNAs support the notion that the Ulvophyceae is sister to the Chlorophyceae.


The green algae are divided into the phyla Streptophyta and Chlorophyta. The Streptophyta (sensu Bremer [1]) encompasses the algae from the class Charophyceae and all land plants, whereas the Chlorophyta (sensu Sluiman [2]) contains algae from the classes Prasinophyceae, Ulvophyceae, Trebouxiophyceae and Chlorophyceae [3]. The basal position of the Prasinophyceae in the Chlorophyta is generally well established, but the branching order of the Ulvophyceae, Trebouxiophyceae and Chlorophyceae (UTC) remains a matter of debate [46]. It has been proposed that a third lineage at the base of the Streptophyta and Chlorophyta is represented by Mesostigma viride [79], an alga traditionally classified within the prasinophytes. This green plant lineage, however, is debated, as some studies suggest that Mesostigma is an early offshoot of the phylum Streptophyta [1012].

Investigations of chloroplast DNA (cpDNA) from green algae representing each of the five recognized classes have revealed that the genomes of the charophyte Chaetosphaeridium globosum [13] and the prasinophytes Mesostigma [7] and Nephroselmis olivacea [14] are highly similar to those of land plants. Like most land plants cpDNAs, these green algal genomes are partitioned into a quadripartite architecture by two copies of a large inverted repeat (IR) separating small (SSC) and large (LSC) single copy regions. Most notably, the great majority of the genes occupying a given single copy region in prasinophyte genomes map to the same single copy region in Chaetosphaeridium and land plant cpDNAs. The increased structural stability of the chloroplast genome conferred by the IR sequence has been hypothesized to limit gene exchanges between the SSC and LSC regions [15]. The IR region readily expands or contracts and thus can easily gain or lose genes from the neighbouring single copy regions through a process known as the ebb and flow [16]. Despite its variable gene content, the IR always features the ribosomal RNA (rRNA) operon (rrs-I(gau)-A(ugc)-rrl-rrf) and this operon is always transcribed toward the SSC region. In addition to their characteristic pattern of gene partitioning, prasinophyte and streptophyte chloroplast genomes share a number of features that were most probably inherited from the progenitor of all green plant cpDNAs. First, they have retained several gene clusters that date back to the cyanobacterial ancestor of all chloroplasts. Second, their genes are densely packed and their intergenic regions virtually lack short dispersed repeats (SDRs). Finally, with 128 to 137 genes, their gene repertoire is one of the largest among green plant cpDNAs.

In contrast, the chloroplast genome has been substantially reorganized in the UTC. The quadripartite architecture has been lost from the genome of the trebouxiophyte Chlorella vulgaris [17] following the disappearance of one copy of the IR sequence. Although the quadripartite architecture has been retained in the genome of the ulvophyte Pseudendoclonium akinetum [6], the IR sequence is atypical in featuring a rRNA operon transcribed towards the LSC region [6]. In addition, the pattern of gene partitioning within the SSC/LSC regions of Pseudendoclonium cpDNA deviates significantly from those found in its prasinophyte and land plant counterparts; the small single copy region of this ulvophyte genome includes 14 genes that are usually located within the LSC region. In the chlorophycean alga Chlamydomonas reinhardtii [18], the two single copy regions are similar in size and the genes are so thoroughly scrambled that no distinction is possible between the SSC and LSC regions. The Chlorella, Pseudendoclonium and Chlamydomonas chloroplast genomes have lost many of the ancestral gene clusters that are shared between Mesostigma and Nephroselmis cpDNAs, feature a reduced gene content (from 94 genes in Chlamydomonas to 112 genes in Chlorella) compared to prasinophyte and streptophyte genomes, and contain SDRs in their intergenic regions. The low density of coding sequences in these genomes is explained not only by the smaller number of genes but also by the expansion of intergenic regions. Moreover, unlike Mesostigma and Nephroselmis cpDNAs, the chloroplast genomes of the three UTC algae have acquired group I introns (from three in Chlorella to 27 in Pseudendoclonium) and group II introns (two in Chlamydomonas).

To gain insights into the nature of the events that led to the reorganization of the chloroplast genome in the Ulvophyceae, we have determined the complete cpDNA sequence of Oltmannsiellopsis viridis. This marine unicellular green alga exhibits a counterclockwise arrangement of basal bodies [19, 20] and a single cup-shaped chloroplast [20]. Previously classified in the Chlorophyceae [19, 21], Oltmannsiellopsis is currently considered to be the type species of the order Oltmannsiellopsidales (Ulvophyceae) [22]. The Oltmannsiellopsidales have been shown to branch at the base of the Ulvophyceae [4] and have been used as outgroup for phylogenetic analyses of the Ulvophyceae [2325]. Considering that Pseudendoclonium represents a distinct, early diverging lineage of the Ulvophyceae (Ulotrichales, see supplementary Figure S1 in [6]), identification of the set of features common to Oltmannsiellopsis and Pseudendoclonium cpDNAs should throw light into the chloroplast genome architecture of the earliest diverging ulvophytes and, accordingly, into the cpDNA changes that occurred in the separate lineages leading to Oltmannsiellopsis and Pseudendoclonium. We found that the IR-containing genome of Oltmannsiellopsis differs considerably from its Pseudendoclonium and other chlorophyte counterparts in intron content and gene order, but shares closer similarities with Pseudendoclonium cpDNA in terms of quadripartite architecture, gene content and gene density. In the context of the debate concerning the branching order of the UTC lineages, the predicted architecture of the chloroplast genome of the earliest members of the Ulvophyceae strengthens the notion that this lineage is sister to the Chlorophyceae [5, 6].

Results and discussion

General features

Table 1 compares the general features of Oltmannsiellopsis cpDNA [GenBank: DQ291132] with those of the four chlorophyte cpDNAs completely sequenced thus far, i.e. the genomes of Nephroselmis [GenBank:NC_000927], Chlorella [GenBank:NC_001865], Pseudendoclonium [GenBank:AY835431] and Chlamydomonas [GenBank:NC_005353]. At 59.5%, the overall A+T content of Oltmannsiellopsis cpDNA is similar to that of Nephroselmis cpDNA but is significantly lower than those of the three previously sequenced UTC genomes. The Oltmannsiellopsis genome maps as a circular molecule of 151,933 bp (Figure 1) and contains 105 genes. Two copies of an IR sequence of 18,510 bp, each encoding ten genes, are separated from one another by unequal single copy regions, designated SC1 and SC2. Like other UTC cpDNAs, the Oltmannsiellopsis genome is less densely packed with coding sequences than Mesostigma and Nephroselmis cpDNAs; at 59.2%, its density of coding sequences is similar to those of Chlorella and Pseudendoclonium cpDNAs. Intergenic spacers in Oltmannsiellopsis cpDNA feature SDRs and have an average size of 512 bp, a value comparable to that observed for Pseudendoclonium cpDNA (600 bp). A total of five introns, all of which belong to the group I family, were identified in Oltmannsiellopsis cpDNA.

Table 1 General features of Oltmannsiellopsis and other chlorophyte cpDNAs
Figure 1

Gene maps of Oltmannsiellopsis and other chlorophyte cpDNAs. Genes (filled boxes) outside each map are transcribed clockwise. The transcription direction of the rRNA genes is indicated by arrows. Genes shown in yellow, blue and red map to the IR, LSC and SSC regions in Mesostigma cpDNA, respectively. On the Oltmannsiellopsis map, genes characteristic of the LSC region that reside in the single copy region corresponding to SSC in both Oltmannsiellopsis and Pseudendoclonium cpDNAs are denoted by asterisks. Genes absent from Mesostigma cpDNA are shown in grey. tRNA genes are indicated by the one-letter amino acid code followed by the anticodon in parentheses (Me, elongator methionine; Mf, initiator methionine). A total of five introns (open boxes) were identified, some of which feature ORFs (narrow boxes).

Gene and intron contents

The gene content of Oltmannsiellopsis cpDNA is intermediate between those of Chlorella and Chlamydomonas cpDNAs (Table 1). Although Oltmannsiellopsis and Pseudendoclonium cpDNAs encode the same number of genes, these genomes differ slightly in their gene repertoire (Table 2). Oltmannsiellopsis cpDNA has retained all three chl genes that are missing from Pseudendoclonium cpDNA but has lost ycf62, trnL(caa) and trnR(ccg). Relative to Chlorella cpDNA, the genomes of Oltmannsiellopsis, Pseudendoclonium and Chlamydomonas are missing a set of five genes, i.e. cysA, cyst, and three tRNA genes (trnL(gag), trnS(gga) and trnT(ggu)) (Table 2). The absence of three genes (ycf62, trnL(caa) and trnR(ccg)) is uniquely shared by Oltmannsiellopsis and Chlamydomonas cpDNAs, whereas no specific gene loss is shared by Pseudendoclonium and Chlamydomonas cpDNAs. Both Oltmannsiellopsis and Pseudendoclonium cpDNAs have retained the trnR(ccu) gene, which is absent from all other completely sequenced chlorophyte cpDNAs.

Table 2 Differences between the gene repertoires of Oltmannsiellopsis and other UTC algal cpDNAs

As in the UTC chloroplast genomes previously investigated, the coding regions of several genes in Oltmannsiellopsis cpDNA are expanded relative to their Mesostigma counterparts [6] (Table 3). However, most of the gene expansions in Oltmannsiellopsis are less extensive than those in Pseudendoclonium; only cemA displays a longer coding sequence than its Pseudendoclonium homologue.

Table 3 Compared sizes of expanded genes in Oltmannsiellopsis and other UTC algal cpDNAs

Our finding of five group I introns in Oltmannsiellopsis cpDNA contrasts sharply with the 27 group I introns found in Pseudendoclonium cpDNA [6] (Table 1). The lower abundance of introns in Oltmannsiellopsis cpDNA mainly accounts for the smaller size of this genome relative to Pseudendoclonium cpDNA. The Oltmannsiellopsis introns interrupt three genes (petB, psbA, and rrl) found in the IR (Table 4). The petB and psbA genes each contain one intron, whereas three introns are present in rrl. All five introns, with the exception of the petB intron, are positionally and structurally homologous to previously reported introns in green plant cpDNAs (Table 5). While homologues of the Oltmannsiellopsis psbA intron are present in Pseudendoclonium and Chlamydomonas, homologues of the three rrl introns are found in a larger diversity of green plants. Considering that these homologous introns have been identified in UTC lineages, they could have been inherited by vertical inheritance from the last common ancestor of UTC algae; however, the finding that they potentially code for homing endonucleases of the LAGLIDADG or GIY-YIG families (Table 4) does not allow us to exclude the possibility that they were acquired by horizontal transfer. Although most of the 16 group I introns in Pseudendoclonium cpDNA have no homologues at identical cognate sites in other chloroplast genomes, their close structural and sequence similarities together with their absence from Oltmannsiellopsis cpDNA suggest that they arose from intragenomic proliferation in the lineage leading to Pseudendoclonium [6]. Note that Blast searches of the Oltmannsiellopsis petB intron sequence against the GenBank database failed to detect any homologous intron in other organisms.

Table 4 Group I introns in Oltmannsiellopsis cpDNA
Table 5 Group I introns at identical gene locations in Oltmannsiellopsis cpDNA, other green algal cpDNAs and land plant cpDNAs

Genome structure and gene partitioning

The pattern of gene partitioning within the single copy regions of Oltmannsiellopsis cpDNA differs substantially from the ancestral partitioning pattern observed for Mesostigma, Nephroselmis and streptophyte cpDNAs (Figure 1). The great majority of the 30 genes found in the SC1 region of Oltmannsiellopsis are typically found in the ancestral LSC region, whereas the SC2 region contains 52 genes characteristic of the ancestral LSC region in addition to ten genes characteristic of the ancestral SSC region. Interestingly, SC2 includes 12 of the 14 LSC genes that have been transferred to the SSC region in Pseudendoclonium cpDNA. The two exceptional Pseudendoclonium genes that have no homologues in Oltmannsiellopsis SC2 are trnH(gug) and trnL(caa); the trnH(gug) gene resides in the SC1 region of Oltmannsiellopsis, whereas trnL(caa) has been lost from Oltmannsiellopsis cpDNA. Considering the gene contents of the Oltmannsiellopsis single copy regions, it appears inappropriate to label these regions according to their sizes. Although SC1 is smaller than SC2, it likely corresponds to the ancestral LSC region, and SC2 is apparently derived from the ancestral SSC region.

The IR sequence in Oltmannsiellopsis cpDNA is about 12 kb larger than that in Pseudendoclonium cpDNA and contains five genes in addition to those found in the rRNA operon (Figure 1). At 18,510 bp, the IR sequence of Oltmannsiellopsis is similar in size to that of Chlamydomonas (Table 1). Both IR junctions in Oltmannsiellopsis cpDNA encompass genes (cemA and ftsH) of which the coding sequences expand into the single copy regions. As in the Pseudendoclonium IR, the Oltmannsiellopsis rRNA genes are transcribed towards the single copy region carrying the genes that map to the LSC in prasinophyte and streptophyte cpDNAs. In contrast, the rRNA operon is transcribed toward the SSC region in Nephroselmis and streptophyte cpDNAs. The orientation of the rRNA operon cannot be established in Chlamydomonas cpDNA owing to the extensively scrambled single copy regions, and this orientation remains unknown in Chlorella cpDNA because of the IR loss.

Considering that Oltmannsiellopsis and Pseudendoclonium represent distinct, early diverging lineages of the Ulvophyceae, the striking similarities between the quadripartite architectures of Oltmannsiellopsis and Pseudendoclonium cpDNAs suggest that both the atypical gene partitioning pattern and unusual orientation of the IR were characteristic of the chloroplast genome of earliest-diverging ulvophytes. Our data predict that the SSC region of the last common ancestor of Oltmannsiellopsis and Pseudendoclonium cpDNAs featured 12 of the genes usually found in the LSC region in Nephroselmis and streptophyte cpDNAs, whereas the LSC region contained exclusively genes characteristic of the ancestral LSC region. Consequently, in the lineage leading to Pseudendoclonium, two extra genes were transferred to the SSC region, whereas 40 additional genes migrated to this region in the Oltmannsiellopsis lineage. Although the mechanisms underlying these gene migrations between single copy regions remain unknown, they probably involved intramolecular or intermolecular recombination events. The analysis of conserved gene clusters reported below clearly indicates that several genes were transferred together in the course of these migrations.

Genes have been more extensively shuffled between the two single copy regions in Chlamydomonas cpDNA (Figure 1). It can be envisioned that during the evolution of ulvophytes and chlorophycean green algae, the ancestral pattern of gene partitioning was disrupted in successive steps, with a Pseudendoclonium-like organization evolving into an Oltmannsiellopsis-like organization, leading ultimately to the extensive scrambling of genes observed in Chlamydomonas. Given the absence of the IR from the Chlorella genome, it is very difficult to ascertain whether the transcription direction of the rRNA operon changed and whether genes were relocated from one genomic region to another during the evolution of trebouxiophytes. Loss of the IR is usually associated with many gene rearrangements [15]; in the case of Chlorella cpDNA, however, all the genes usually found in the ancestral SSC region have remained clustered, with the exception of three genes (psaC, ycf20 and trnL(uag)) (Figure 1). Investigations of IR-containing chloroplast genomes from distinct trebouxiophyte lineages will be required to test whether some of the gene relocations identified here in both Oltmannsiellopsis and Pseudendoclonium cpDNAs originated from the common ancestor of UTC algae.

Gene clustering

The overall gene organization of Oltmannsiellopsis cpDNA differs extensively from that of its Pseudendoclonium homologue and, surprisingly, more closely resembles that of Chlorella cpDNA (Figure 2). Oltmannsiellopsis and Chlorella cpDNAs share 21 blocks of colinear sequences that contain a total of 65 genes, whereas Oltmannsiellopsis and Pseudendoclonium cpDNAs have in common 18 blocks containing 55 genes. Only eight blocks containing 19 genes are conserved in the Oltmannsiellopsis and Chlamydomonas genomes.

Figure 2

Gene clusters shared between Oltmannsiellopsis and other UTC algal cpDNAs. Shared clusters are shown on the Oltmannsiellopsis gene map as alternating series of green and red boxes. Genes located outside conserved clusters are shown in grey. Genes missing from Chlamydomonas, Pseudendoclonium and Chlorella are represented in beige. The extent of the IR and transcription direction of the rRNA genes are denoted by arrows. Genes outside the map are transcribed clockwise.

Many of the 24 ancestral gene clusters shared by Mesostigma and Nephroselmis cpDNAs have been disrupted during the evolution of the UTC green algae. In this study, we have analyzed 19 ancestral clusters; the five remaining ones could not be investigated because the genes they contain have been lost from UTC cpDNAs (Figure 3). All 19 clusters have been broken at least in one occasion during the evolution of the UTC algae. With only 12 breakpoints, Chlorella cpDNA displays the strongest conservation of ancestral clusters. With 20 breakpoints, Oltmannsiellopsis cpDNA occupies a median position between Chlorella and Pseudendoclonium (24 breakpoints) cpDNAs, whereas Chlamydomonas cpDNA reveals twice as many breakpoints (42 breakpoints). The Chlamydomonas, Oltmannsiellopsis and Pseudendoclonium genomes share five breakpoints that are missing in Chlorella cpDNA. Aside from these breakpoints, Pseudendoclonium and Chlamydomonas cpDNAs share six breakpoints that are absent from Oltmannsiellopsis and Chlorella cpDNAs. There is no breakpoint exclusive to the Oltmannsiellopsis and Chlamydomonas genomes.

Figure 3

Fragmented ancestral gene clusters in the cpDNAs of UTC algae. The indicated clusters are found in both Mesostigma and Nephroselmis cpDNAs. Note that rpl22 has not been represented in the large ribosomal protein cluster because it is present only in Mesostigma cpDNA (between rps19 and rps3). Sites of fragmentation are denoted by arrowheads of different shades above the clusters: Chlamydomonas, filled arrowheads; Pseudendoclonium, dark grey arrowheads; Oltmannsiellopsis, light grey arrowheads; and Chlorella, open arrowheads. Genes missing from Chlamydomonas, Pseudendoclonium, Oltmannsiellopsis and Chlorella are indicated by squares, circles, asterisks and double dagger, respectively. Gene polarities are not shown.

Two ancestral clusters display breakpoints that are unique to the Ulvophyceae. The almost universally conserved psbB-psbT-psbN-psbH cluster was fragmented at the 5' end of psbN, creating two separate pieces, each encoding a pair of genes, in Oltmannsiellopsis cpDNA. In the Pseudendoclonium lineage, the introduction of an additional breakpoint on the opposite side of psbN led to the relocation of this gene on the DNA strand encoding psbB, psbT and psbH, without any change in gene order. In the Oltmannsiellopsis lineage, three breakpoints occurred in the ancestral rRNA operon to generate a new transcription unit in which the order of the trnA(ugc) and trnI(gau) genes has been reversed. Rearranged rRNA operons have been reported for the cpDNAs of the trebouxiophyte Chlorella ellipsoidea [26] and the ulvophyte Codium fragile [27]; however, in these cases, the ancestral rRNA operon was split into separate fragments that are transcribed from different promoters.

In terms of derived gene clusters, Oltmannsiellopsis cpDNA is most similar to Chlorella cpDNA (Figure 4). A derived cluster is defined here as a group of genes with the same relative polarities in two or more UTC genomes, but absent from Mesostigma and Nephroselmis cpDNAs. Oltmannsiellopsis cpDNA shares five derived clusters with its Chlorella homologue, whereas Pseudendoclonium cpDNA shares three clusters, one of which is missing from Oltmannsiellopsis. Of the four derived clusters common to Oltmannsiellopsis and Pseudendoclonium cpDNAs, none is found in Chlamydomonas cpDNA.

Figure 4

Derived gene clusters shared between the cpDNAs of UTC algae. Filled/open boxes represent the presence/absence of clusters. Gene polarities are not shown.

We estimated that a minimum of 50 inversions would be required to transform the gene organization of Oltmannsiellopsis cpDNA into that of any other chlorophyte genome (Table 6). Comparative analyses of cpDNAs from land plants [15] and from closely related chlamydomonads [28, 29] suggest that inversions represent the predominant mechanism of chloroplast genome rearrangements in green plants. However, inversions might be not the only mutational events causing gene order changes in chlorophytes cpDNAs, as transpositions have been proposed to account for some of the rearrangements observed in Campanulaceae [30] and in subclover [31] cpDNAs.

Table 6 Minimal numbers of inversions accounting for gene rearrangements between green algal cpDNAs

Repeated elements

A large number of SDR elements are found in Oltmannsiellopsis cpDNA (Figure 5). Although these elements reside predominantly within intergenic spacers and introns, a few copies populate the coding regions of cemA, chlB, chlL, chlN, ftsH, rpoB, rpoC1 and rpoC2. The most abundant elements can be classified into five groups of non-overlapping repeat units (A through E) on the basis of their primary sequences (Table 7). Their sizes range from 7–21 bp and their copy numbers vary from 17 to more than 250. The sequence of repeat unit A or B is most often linked to the reverse complement of the same sequence, thus forming perfect palindromes or putative stem-loop structures with a loop of two A or two T (Figure 6). In some instances, the palindromes or stem portions of the stem-loop structures are extended by the addition of less frequent repeats. Furthermore, a few copies of repeat units A and B occur as solitary sequences, representing probably degenerated versions of the more common arrangements featuring palindromes or stem-loop structures. Repeat unit C can form stem-loop structures, with a loop of variable size. Although repeat units D and E are not associated with stem-loop structures, they reside in the vicinity of other repeated elements.

Figure 5

Positions of SDR elements in Oltmannsiellopsis cpDNA. The Oltmannsiellopsis cpDNA sequence was aligned against itself using PipMaker. Regions containing SDRs can be identified as clusters of dots. Similarities between aligned regions are shown as average percent identity (between 50 and 100% identity). Genes and their polarities are denoted by horizontal arrows and coding sequences are represented by filled boxes.

Table 7 SDR units in Oltmannsiellopsis cpDNA
Figure 6

Predicted secondary structures formed by SDR units A and B in Oltmannsiellopsis cpDNA.

The SDRs in Oltmannsiellopsis cpDNA do not closely resemble those present in other UTC cpDNAs. The Oltmannsiellopsis repeats are biased in G+C, whereas the Chlorella repeats show a bias in A+T. The Pseudendoclonium and Chlamydomonas SDRs are also rich in G+C, but their sequences share no obvious similarities with the Oltmannsiellopsis repeats. This lack of sequence similarities between SDRs derived from distinct UTC genomes suggests that SDRs have been acquired independently in UTC lineages. However, the alternative hypothesis that SDRs were transmitted vertically cannot be excluded if we assume that these elements evolve at a very fast pace. Studies of cpDNAs from closely related UTC taxa will be required to distinguish between these two hypotheses.

SDRs have most probably played a major role in remodelling the chloroplast genome in UTC lineages. A correlation has been previously observed between the abundance of SDRs and the extent of gene rearrangements in UTC algal genomes [6]. This correlation still holds with the addition of Oltmannsiellopsis chloroplast genome sequence. The abundance of SDR elements in Oltmannsiellopsis cpDNA is comparable to that observed in Pseudendoclonium cpDNA (Figure 7) and genes have been rearranged to a similar extent in both genomes (Table 6). SDRs in green plant cpDNAs could serve as hot spots for nonhomologous recombinational events and lead to inversions and transpositions [15, 30, 31].

Figure 7

Densities of SDR elements in Oltmannsiellopsis and other chlorophyte cpDNAs as revealed by REPuter. Repeated elements with identical sequences are connected on the circular representations of the genomes. Repeats larger than 30 bp and 45 bp are shown on the top and bottom panels, respectively. For these analyses, one copy of the IR sequence was deleted from the Nephroselmis, Pseudendoclonium, Oltmannsiellopsis and Chlamydomonas genomes.


Although the Oltmannsiellopsis chloroplast genome differs considerably from its Pseudendoclonium counterpart at the levels of intron content and gene order, the two ulvophyte genomes share similarities in gene content and quadripartite architecture. We conclude that the chloroplast genome of the last common ancestor of Oltmannsiellopsis and Pseudendoclonium contained a minimum of 108 genes, was loosely packed with coding sequences, carried only a few group I introns, and featured a quadripartite architecture that deviates from the ancestral type displayed by Mesostigma and Nephroselmis cpDNAs with regard to the transcription direction of the rRNA genes and the gene contents of the single copy regions. Given the phylogenetic positions of Oltmannsiellopsis and Pseudendoclonium, these genomic characters were undoubtedly present in the earliest-diverging members of the Ulvophyceae. Numerous changes were experienced by the chloroplast genome in the lineages leading to Oltmannsiellopsis and Pseudendoclonium; these include contraction/expansion of the IR, migration of genes from the ancestral LSC region toward the single copy region corresponding to the SSC, gene losses, intron gains/losses, and gene rearrangements within the IR and each of the single copy regions. Considering that the chloroplast genome of Codium fragile (Ulvales) is greatly reduced in size (only 89 kbp) and lacks an IR [27], many additional chloroplast gene losses and rearrangements probably occurred in some lineages of the Ulvophyceae.

Our comparative analysis of the Oltmannsiellopsis chloroplast genome with its chlorophyte counterparts strengthens the idea that the chloroplast genomes of early-diverging ulvophytes occupy an intermediate position between those of the trebouxiophyte Chlorella and the chlorophycean green alga Chlamydomonas with respect to the retention of ancestral features [6]. In the context of the debate on the branching order of UTC lineages [46], this analysis provides further support for the published phylogenetic analysis of mitochondrial gene sequences identifying the Trebouxiophyceae as a basal lineage relative to the Ulvophyceae and Chlorophyceae [5].


Isolation and sequencing of OltmannsiellopsiscpDNA

Oltmannsiellopsis viridis was obtained from the National Institute for Environmental Studies of Japan (NIES 360) and grown in K medium [32] under 12 h light/dark cycles. Organellar DNA was isolated and sequenced as described previously [5]. Sequences were edited and assembled with SEQUENCHER 4.2.1 (GeneCodes, Ann Arbor, MI). The fully annotated chloroplast genome sequence has been deposited in [GenBank:DQ291132].

Sequence analyses

Genes and ORFs were identified as described previously [6]. Homologous introns were detected by BLASTN searches [33] against the non-redundant database of National Center for Biotechnology Information using an E value threshold of 1 × 10-4. Homologous introns inserted at identical positions within the same gene were identified by manual screening of the GOBASE database [34].

Repeated sequences were mapped with PipMaker [35], identified with REPuter 2.74 [36] and classified with REPEATFINDER [37], using the default parameters. Sequences clustered with REPEATFINDER were aligned manually using BIOEDIT 7.0.1 [38], and non-overlapping SDR units were identified by manual screening of the alignment. Numbers of SDR units were determined with FINDPATTERNS of the GCG Wisconsin Package version 10.2 (Accelrys, Burlington, Mass.), using 100% or 90% sequence identity. Putative stem-loop structures and degenerate repeats were identified using PALINDROME and ETANDEM in EMBOSS 2.9.0 [39], respectively. The density of repeated elements in a given chloroplast genome was assessed with REPuter 2.74 [36] using the -f (forward), -p (palindromic), and -allmax options at minimum lengths (-l) of 30 bp and 45 bp. For the analyses involving IR-containing genomes, one copy of the IR sequence was deleted. Circle graphs generated by REPuter were screen-captured at 300 dpi and converted to black and white illustrations with GIMP 2.0 [40]. Repeated elements in different cpDNAs were compared using Vmatch [41] and GenAlyzer 0.81 b [42].

The GRIMM web server [43] was used to infer the minimal number of gene permutations by inversions in pairwise comparisons of chloroplast genomes. Because GRIMM cannot deal with duplicated genes and requires that the compared genomes have the same gene content, genes within one of the two copies of the IR were excluded and only the genes common to all the compared genomes were analysed. The data set used in the comparative analyses reported in Table 6 contained 90 genes; the three exons of the trans-spliced psaA gene were coded as distinct fragments (for a total of 92 gene loci).



chloroplast DNA


inverted repeat


large single copy


open reading frame


ribosomal rRNA


short dispersed repeat


small single copy




  1. 1.

    Bremer K: Summary of green plant phylogeny and classification. Cladistics. 1985, 1: 369-385.

  2. 2.

    Sluiman HJ: The green algal class Ulvophyceae. An ultrastructural survey and classification. Crypt Bot. 1989, 1: 83-94.

  3. 3.

    Lewis LA, McCourt RM: Green algae and the origin of land plants. Am J Bot. 2004, 91 (10): 1535-1556.

  4. 4.

    Friedl T, O'Kelly CJ: Phylogenetic relationships of green algae assigned to the genus Planophila (Chlorophyta): evidence from 18S rDNA sequence data and ultrastructure. Eur J Phycol. 2002, 37: 373-384. 10.1017/S0967026202003712.

  5. 5.

    Pombert JF, Otis C, Lemieux C, Turmel M: The complete mitochondrial DNA sequence of the green alga Pseudendoclonium akinetum (Ulvophyceae) highlights distinctive evolutionary trends in the Chlorophyta and suggests a sister-group relationship between the Ulvophyceae and Chlorophyceae. Mol Biol Evol. 2004, 21 (5): 922-935. 10.1093/molbev/msh099.

  6. 6.

    Pombert JF, Otis C, Lemieux C, Turmel M: The Chloroplast Genome Sequence of the Green Alga Pseudendoclonium akinetum (Ulvophyceae) Reveals Unusual Structural Features and New Insights into the Branching Order of Chlorophyte Lineages. Mol Biol Evol. 2005, 22 (9): 1903-1918. 10.1093/molbev/msi182.

  7. 7.

    Lemieux C, Otis C, Turmel M: Ancestral chloroplast genome in Mesostigma viride reveals an early branch of green plant evolution. Nature. 2000, 403 (6770): 649-652. 10.1038/35001059.

  8. 8.

    Turmel M, Ehara M, Otis C, Lemieux C: Phylogenetic relationships among Streptophytes as inferred from chloroplast small and large subunit rRNA gene sequences. J Phycol. 2002, 38: 364-375. 10.1046/j.1529-8817.2002.01163.x.

  9. 9.

    Turmel M, Otis C, Lemieux C: The complete mitochondrial DNA sequence of Mesostigma viride identifies this green alga as the earliest green plant divergence and predicts a highly compact mitochondrial genome in the ancestor of all green plants. Mol Biol Evol. 2002, 19 (1): 24-38.

  10. 10.

    Bhattacharya D, Weber K, An SS, Berning-Koch W: Actin phylogeny identifies Mesostigma viride as a flagellate ancestor of the land plants. J Mol Evol. 1998, 47 (5): 544-550. 10.1007/PL00006410.

  11. 11.

    Marin B, Melkonian M: Mesostigmatophyceae, a new class of streptophyte green algae revealed by SSU rRNA sequence comparisons. Protist. 1999, 150 (4): 399-417.

  12. 12.

    Karol KG, McCourt RM, Cimino MT, Delwiche CF: The closest living relatives of land plants. Science. 2001, 294: 2351-2353. 10.1126/science.1065156.

  13. 13.

    Turmel M, Otis C, Lemieux C: The chloroplast and mitochondrial genome sequences of the charophyte Chaetosphaeridium globosum: insights into the timing of the events that restructured organelle DNAs within the green algal lineage that led to land plants. Proc Natl Acad Sci USA. 2002, 99 (17): 11275-11280. 10.1073/pnas.162203299.

  14. 14.

    Turmel M, Otis C, Lemieux C: The complete chloroplast DNA sequence of the green alga Nephroselmis olivacea: insights into the architecture of ancestral chloroplast genomes. Proc Natl Acad Sci USA. 1999, 96 (18): 10248-10253. 10.1073/pnas.96.18.10248.

  15. 15.

    Palmer JD: Plastid chromosomes: structure and evolution. The Molecular Biology of Plastids Cell Culture and Somatic Cell Genetics of Plants. Edited by: Bogorad L, Vasil I. 1991, San Diego: Academic Press, 7A: 5-53.

  16. 16.

    Goulding SE, Olmstead RG, Morden CW, Wolfe KH: Ebb and flow of the chloroplast inverted repeat. Mol Gen Genet. 1996, 252 (1–2): 195-206. 10.1007/BF02173220.

  17. 17.

    Wakasugi T, Nagai T, Kapoor M, Sugita M, Ito M, Ito S, Tsudzuki J, Nakashima K, Tsudzuki T, Suzuki Y, Hamada A, Ohta T, Inamura A, Yoshinaga K, Sugiura M: Complete nucleotide sequence of the chloroplast genome from the green alga Chlorella vulgaris: the existence of genes possibly involved in chloroplast division. Proc Natl Acad Sci USA. 1997, 94 (11): 5967-5972. 10.1073/pnas.94.11.5967.

  18. 18.

    Maul JE, Lilly JW, Cui L, dePamphilis CW, Miller W, Harris EH, Stern DB: The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats. Plant Cell. 2002, 14 (11): 2659-2679. 10.1105/tpc.006155.

  19. 19.

    Chihara M, Inouye I, Takahata N: Oltmannsiellopsis, a new genus of marine flagellate (Dunaliellaceae, Chlorophyceae). Arch Protistenkd. 1986, 132: 313-324.

  20. 20.

    Lokhorst GM, Star W: The flagellar apparatus in the marine flagellate algal genus Oltmannsiellopsis (Dunaliellales, Chlorophyceae). Arch Protistenkd. 1993, 143: 13-32.

  21. 21.

    Hargraves PE, Steele RL: Morphology and ecology of Oltmannsiella virida, sp. nov. (Chlorophyceae: Volvocales). Phycologia. 1980, 19: 96-102.

  22. 22.

    Nakayama T, Watanabe S, Inouye I: Phylogeny of wall-less green flagellates inferred from 18S rDNA sequence data. Phycological Research. 1996, 44: 151-161. 10.1111/j.1440-1835.1996.tb00044.x.

  23. 23.

    O'Kelly CJ, Wysor B, Bellows WK: Gene sequence diversity and the phylogenetic position of algae assigned to the genera Phaeophila and Ochlochaete (Ulvophyceae, Chlorophyta). J Phycol. 2004, 40: 789-799. 10.1111/j.1529-8817.2004.03204.x.

  24. 24.

    O'Kelly CJ, Wysor B, Bellows WK: Collinsiella (Ulvophyceae, Chlorophyta) and other ulotrichalean taxa with shell-boring sporophytes form a monophyletic clade. Phycologia. 2004, 43 (1): 41-49.

  25. 25.

    O'Kelly CJ, Bellows WK, Wysor B: Phylogenetic position of Bolbocoleon piliferum (Ulvophyceae, Chlorophyta): Evidence from reproduction, zoospore and gamete ultrastructure, and small subunit rRNA gene sequences. J Phycol. 2004, 40: 209-222. 10.1111/j.1529-8817.2004.03204.x.

  26. 26.

    Yamada T, Shimaji M: Splitting of the ribosomal RNA operon on chloroplast DNA from Chlorella ellipsoidea. Mol Gen Genet. 1987, 208 (3): 377-383. 10.1007/BF00328127.

  27. 27.

    Manhart JR, Kelly K, Dudock BS, Palmer JD: Unusual characteristics of Codium fragile chloroplast DNA revealed by physical and gene mapping. Mol Gen Genet. 1989, 216 (2–3): 417-421. 10.1007/BF00334385.

  28. 28.

    Boudreau E, Turmel M: Gene rearrangements in Chlamydomonas chloroplast DNAs are accounted for by inversions and by the expansion/contraction of the inverted repeat. Plant Mol Biol. 1995, 27 (2): 351-364. 10.1007/BF00020189.

  29. 29.

    Boudreau E, Turmel M: Extensive gene rearrangements in the chloroplast DNAs of Chlamydomonas species featuring multiple dispersed repeats. Mol Biol Evol. 1996, 13 (1): 233-243.

  30. 30.

    Cosner ME, Raubeson LA, Jansen RK: Chloroplast DNA rearrangements in Campanulaceae: phylogenetic utility of highly rearranged genomes. BMC Evol Biol. 2004, 4 (1): 27-10.1186/1471-2148-4-27.

  31. 31.

    Milligan BG, Hampton JN, Palmer JD: Dispersed repeats and structural reorganization in subclover chloroplast DNA. Mol Biol Evol. 1989, 6 (4): 355-368.

  32. 32.

    Keller MD, Selvin RC, Claus W, Guillard RRL: Media for the culture of oceanic ultraphytoplankton. J Phycol. 1987, 23: 633-638.

  33. 33.

    Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215 (3): 403-410. 10.1006/jmbi.1990.9999.

  34. 34.

    O'Brien EA, Badidi E, Barbasiewicz A, deSousa C, Lang BF, Burger G: GOBASE – a database of mitochondrial and chloroplast information. Nucleic Acids Res. 2003, 31 (1): 176-178. 10.1093/nar/gkg090.

  35. 35.

    Schwartz S, Zhang Z, Frazer KA, Smit A, Riemer C, Bouck J, Gibbs R, Hardison R, Miller W: PipMaker – a web server for aligning two genomic DNA sequences. Genome Res. 2000, 10 (4): 577-586. 10.1101/gr.10.4.577.

  36. 36.

    Kurtz S, Choudhuri JV, Ohlebusch E, Schleiermacher C, Stoye J, Giegerich R: REPuter: the manifold applications of repeat analysis on a genomic scale. Nucleic Acids Res. 2001, 29 (22): 4633-4642. 10.1093/nar/29.22.4633.

  37. 37.

    Volfovsky N, Haas BJ, Salzberg SL: A clustering method for repeat analysis in DNA sequences. Genome Biol. 2001, 2 (8): Research0027-10.1186/gb-2001-2-8-research0027.

  38. 38.

    Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 1999, 41: 95-98.

  39. 39.

    Rice P, Longden I, Bleasby A: EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 2000, 16 (6): 276-277. 10.1016/S0168-9525(00)02024-2.

  40. 40.

    The GNU Image Manipulation Program. []

  41. 41.

    The Vmatch large scale analysis software. []

  42. 42.

    Choudhuri JV, Schleiermacher C, Kurtz S, Giegerich R: GenAlyzer: interactive visualization of sequence similarities between entire genomes. Bioinformatics. 2004, 20 (12): 1964-1965. 10.1093/bioinformatics/bth161.

  43. 43.

    Tesler G: GRIMM: genome rearrangements web server. Bioinformatics. 2002, 18 (3): 492-493. 10.1093/bioinformatics/18.3.492.

  44. 44.

    Michel F, Westhof E: Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis. J Mol Biol. 1990, 216 (3): 585-610. 10.1016/0022-2836(90)90386-Z.

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We are grateful to Charles O'Kelly for his valuable suggestions of candidate taxa for this study, to Patrick Charlebois for his help with the analysis of conserved gene clusters, and to Philippe Beauchamp for his technical assistance in determining the Oltmannsiellopsis cpDNA sequence. We also thank Christian Otis for critical reading of the manuscript. This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (to MT and CL).

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Correspondence to Monique Turmel.

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Authors' contributions

JFP participated in the conception of this study, carried out the genome sequencing, performed all sequence analyses, annotated the genome, generated the figures, and drafted the manuscript. CL and MT conceived the study, contributed to the interpretation of the data, and helped to prepare the manuscript. All authors read and approved the final manuscript.

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Pombert, J., Lemieux, C. & Turmel, M. The complete chloroplast DNA sequence of the green alga Oltmannsiellopsis viridis reveals a distinctive quadripartite architecture in the chloroplast genome of early diverging ulvophytes . BMC Biol 4, 3 (2006).

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  • Inverted Repeat
  • Chloroplast Genome
  • rRNA Operon
  • Inverted Repeat Sequence
  • Intron Content